FastQCFastQC Report
Wed 25 May 2016
SRR1780646_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780646_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70700
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT13971.9759547383309761No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT5340.7553041018387553TruSeq Adapter, Index 12 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC2180.30834512022630833No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1710.24186704384724184No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG1170.16548797736916548No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGCTG150.002216254470.0000121
GCGAGAC207.8773446E-570.011
ATAGCAT207.8773446E-570.03
CGCTGGA207.8773446E-570.017
GAGACGC207.8773446E-570.013
GATAGCA207.8773446E-570.02
AGCCGGA252.3756683E-455.99999623
GGAGCAC252.3756683E-455.99999627
GCAGTAT252.3756683E-455.99999642
ATGTCGC252.3756683E-455.99999637
TTGCGAG252.3756683E-455.9999969
ACGCTGG252.3756683E-455.99999616
GAGCCGG252.3756683E-455.99999622
GAGCACC252.3756683E-455.99999628
GACGCTG252.3756683E-455.99999615
TATGTCG252.3756683E-455.99999636
CGATAGC252.3756683E-455.9999961
AAATAAC200.006925216452.549
GGTTTAC200.006925216452.550
GCCCTCC200.006925216452.523
Produced by FastQC (version 0.11.5)