##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780646_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 70700 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.05940594059406 34.0 34.0 34.0 32.0 34.0 2 33.35528995756719 34.0 34.0 34.0 33.0 34.0 3 33.52987270155587 34.0 34.0 34.0 34.0 34.0 4 33.533422913719946 34.0 34.0 34.0 34.0 34.0 5 33.53388967468175 34.0 34.0 34.0 34.0 34.0 6 37.275954738330974 38.0 38.0 38.0 36.0 38.0 7 36.88188118811881 38.0 37.0 38.0 35.0 38.0 8 36.92142857142857 38.0 37.0 38.0 35.0 38.0 9 36.73772277227723 38.0 37.0 38.0 34.0 38.0 10-11 36.43711456859972 38.0 37.0 38.0 34.0 38.0 12-13 36.310473833097596 38.0 37.0 38.0 34.0 38.0 14-15 36.53869165487977 38.0 37.0 38.0 34.0 38.0 16-17 36.662920792079206 38.0 37.0 38.0 34.0 38.0 18-19 36.6750919377652 38.0 37.0 38.0 34.0 38.0 20-21 36.40462517680339 38.0 37.0 38.0 34.0 38.0 22-23 36.490134370579916 38.0 37.0 38.0 34.0 38.0 24-25 35.86748231966054 38.0 36.5 38.0 32.5 38.0 26-27 36.04689533239038 38.0 37.0 38.0 33.0 38.0 28-29 35.93991513437058 38.0 37.0 38.0 32.5 38.0 30-31 35.64437057991513 38.0 36.5 38.0 31.5 38.0 32-33 36.16330975954738 38.0 37.0 38.0 34.0 38.0 34-35 36.168387553041015 38.0 37.0 38.0 33.0 38.0 36-37 36.29736916548798 38.0 37.0 38.0 34.0 38.0 38-39 35.85666902404526 38.0 36.5 38.0 32.5 38.0 40-41 35.48189533239038 38.0 36.0 38.0 31.0 38.0 42-43 35.91757425742574 38.0 36.5 38.0 32.5 38.0 44-45 36.094342291371994 38.0 37.0 38.0 33.5 38.0 46-47 36.35811881188119 38.0 37.0 38.0 34.0 38.0 48-49 36.16406647807638 38.0 37.0 38.0 34.0 38.0 50-51 36.30707920792079 38.0 37.0 38.0 34.0 38.0 52-53 36.19422206506365 38.0 37.0 38.0 34.0 38.0 54-55 36.3719306930693 38.0 37.0 38.0 34.0 38.0 56-57 36.44477369165488 38.0 37.0 38.0 34.0 38.0 58-59 36.49714285714286 38.0 37.0 38.0 34.0 38.0 60-61 36.172050919377654 38.0 37.0 38.0 33.0 38.0 62-63 36.450968882602545 38.0 37.0 38.0 34.0 38.0 64-65 36.465516265912306 38.0 37.0 38.0 34.0 38.0 66-67 35.82714285714286 38.0 37.0 38.0 31.5 38.0 68-69 35.26088401697312 38.0 36.0 38.0 29.0 38.0 70-71 35.195388967468176 38.0 36.0 38.0 29.0 38.0 72-73 34.813917963224895 38.0 36.0 38.0 26.0 38.0 74-75 35.383479490806224 38.0 36.5 38.0 30.5 38.0 76 34.847340876944834 38.0 36.0 38.0 27.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.592960935463239 1101 2 0.49576445299392446 1101 3 0.20297988655558186 1101 4 0.3901679678900294 1101 5 0.3636194575860543 1101 6 -1.350995500752994 1101 7 0.2705117277834148 1101 8 0.5737489601471282 1101 9 0.19631587437782372 1101 10-11 -0.12796026611171385 1101 12-13 0.5154708944094466 1101 14-15 0.3510827522404938 1101 16-17 -0.3962877958741515 1101 18-19 -0.585147130063298 1101 20-21 -0.151239219198672 1101 22-23 0.4640041549995999 1101 24-25 -1.793945961129971 1101 26-27 -0.6294969762570304 1101 28-29 0.5593311162335652 1101 30-31 0.8762549546879157 1101 32-33 -0.021189517570704197 1101 34-35 0.43543843883759337 1101 36-37 -0.06740203807594014 1101 38-39 -0.19726172807653342 1101 40-41 1.298286991451448 1101 42-43 0.7627391087432827 1101 44-45 0.38139512731535774 1101 46-47 0.7264184959351354 1101 48-49 -1.5521597213320604 1101 50-51 -1.4939752781217948 1101 52-53 -1.772505962429399 1101 54-55 -0.6598868264369813 1101 56-57 -1.0280436364450267 1101 58-59 -1.1761112312968436 1101 60-61 -1.6280418364527804 1101 62-63 -0.7343032307973232 1101 64-65 -1.4780669146614045 1101 66-67 -1.9558750606210538 1101 68-69 -1.5786445569333196 1101 70-71 -1.8119619648064003 1101 72-73 -1.988204397148479 1101 74-75 -1.6960032538719716 1101 76 -1.22957034071608 1102 1 -1.378079727993157 1102 2 -0.06095036776584095 1102 3 0.3128648517776256 1102 4 0.3095767426358833 1102 5 0.09602983746834326 1102 6 0.22906067902228955 1102 7 -0.4853737724841096 1102 8 0.19935088631085307 1102 9 0.12696060417985677 1102 10-11 0.6993203652419524 1102 12-13 0.671904816292809 1102 14-15 -0.12139584594035568 1102 16-17 0.3903628194281481 1102 18-19 -0.10303771326287148 1102 20-21 0.6894844298114862 1102 22-23 0.5283099334907675 1102 24-25 -4.874771268781117 1102 26-27 -1.4190903416930922 1102 28-29 0.014721699433138724 1102 30-31 1.448118250568065 1102 32-33 0.20593728820777812 1102 34-35 0.5404745544074174 1102 36-37 -2.083386521757056 1102 38-39 -0.1643229907838588 1102 40-41 0.713381962023945 1102 42-43 0.587812677496629 1102 44-45 0.814815405539008 1102 46-47 0.989226147085482 1102 48-49 -3.1619443655268213 1102 50-51 -0.7755764017173021 1102 52-53 0.18900420343469904 1102 54-55 0.10626485895627269 1102 56-57 0.5458929606657321 1102 58-59 -0.3810670900448372 1102 60-61 -5.171012676474177 1102 62-63 -2.819208260224819 1102 64-65 -0.5548793812210633 1102 66-67 0.6205361218294598 1102 68-69 -0.8021598057294668 1102 70-71 -1.2932688133885293 1102 72-73 -5.961151160123329 1102 74-75 -3.075517700099901 1102 76 -1.66750506516766 1103 1 0.5586834313581477 1103 2 0.48427020012036337 1103 3 -0.2947623137728286 1103 4 0.18963430614947185 1103 5 0.34288875151051457 1103 6 0.33065474555242247 1103 7 0.7315133698195382 1103 8 0.5380346744328435 1103 9 0.1287871387456363 1103 10-11 0.5944330015401817 1103 12-13 -0.3936013551800457 1103 14-15 -0.33662250227511237 1103 16-17 0.14106852596492558 1103 18-19 0.6271886663242228 1103 20-21 -0.026752355487673185 1103 22-23 0.6416519382508241 1103 24-25 -2.761207865891876 1103 26-27 -0.381549521413028 1103 28-29 0.15149039373766726 1103 30-31 0.8578846755417757 1103 32-33 1.0834902853849542 1103 34-35 0.3255246457341414 1103 36-37 -0.4169504781416222 1103 38-39 -0.22055811559705774 1103 40-41 -0.4228747491068461 1103 42-43 -0.47484299306294986 1103 44-45 1.0268179516174882 1103 46-47 0.7571040460172398 1103 48-49 -2.3361293436637567 1103 50-51 -0.3237166574321435 1103 52-53 0.5379414924146033 1103 54-55 -0.25563805796407024 1103 56-57 0.3170918315352722 1103 58-59 -0.4873797042032493 1103 60-61 -3.67740554745442 1103 62-63 -0.7978295033753113 1103 64-65 0.36747906235009253 1103 66-67 -0.1708791657113622 1103 68-69 0.30928647754944194 1103 70-71 0.5188056870819437 1103 72-73 -4.924883379086079 1103 74-75 -1.8422470962364983 1103 76 -2.9578133620625486 1104 1 -0.07593787406057118 1104 2 0.08207397680344997 1104 3 0.16726560084129716 1104 4 -0.4141475083004451 1104 5 -0.2182257805091865 1104 6 0.1073378325803418 1104 7 0.4803331563548454 1104 8 0.3959215791947486 1104 9 0.7305420648540135 1104 10-11 -0.45757336134980875 1104 12-13 0.3249947039332568 1104 14-15 0.6367970379547714 1104 16-17 0.15505148984012607 1104 18-19 0.7995254889843224 1104 20-21 0.0719750665156127 1104 22-23 0.045105345475789704 1104 24-25 0.7246552293462258 1104 26-27 1.0438661189810645 1104 28-29 1.1303876638526162 1104 30-31 1.0559424546879157 1104 32-33 0.6190634586197632 1104 34-35 -0.13450203735288824 1104 36-37 1.0058866524002497 1104 38-39 1.4150894623996635 1104 40-41 1.9437483009752512 1104 42-43 0.7370694658861368 1104 44-45 0.7816927463629781 1104 46-47 0.8413738530779966 1104 48-49 0.3098194453345968 1104 50-51 0.7924830552115338 1104 52-53 0.49237498995154994 1104 54-55 0.45990484022968303 1104 56-57 0.3901111254597325 1104 58-59 -0.4488046836778068 1104 60-61 0.5523897111662706 1104 62-63 0.4814705787264941 1104 64-65 0.880192013910019 1104 66-67 0.7011189869979972 1104 68-69 1.7271590144952498 1104 70-71 1.5176511304316946 1104 72-73 1.2740723885658127 1104 74-75 0.460618769937561 1104 76 -0.21543343595417497 1105 1 0.6312094031671549 1105 2 0.3892109404998152 1105 3 0.28566494077529114 1105 4 -0.12356376242586009 1105 5 -0.24205003793493063 1105 6 0.25420251904898805 1105 7 0.7543636844076502 1105 8 -0.24202190265344115 1105 9 0.7815940450520316 1105 10-11 0.36694190267660076 1105 12-13 -0.8099062861657558 1105 14-15 0.018191427393723814 1105 16-17 0.20955850304145685 1105 18-19 0.7102618751229315 1105 20-21 0.20770113912287513 1105 22-23 0.2933004774889909 1105 24-25 0.7490467309963833 1105 26-27 0.34197874274344287 1105 28-29 0.8177587447107015 1105 30-31 1.323243403532807 1105 32-33 0.8345389124151481 1105 34-35 -0.7538295951086624 1105 36-37 0.3495593009151037 1105 38-39 -0.05154626367294668 1105 40-41 0.10182895279043436 1105 42-43 -0.017288001110557616 1105 44-45 0.4853849905873986 1105 46-47 0.646473894332118 1105 48-49 -0.3320689622561588 1105 50-51 0.6539207614821692 1105 52-53 0.38346409886244004 1105 54-55 0.7602864408897503 1105 56-57 0.5211961089580868 1105 58-59 0.11676359189975472 1105 60-61 0.8323247359187462 1105 62-63 -0.24264245757713354 1105 64-65 0.502690982556885 1105 66-67 0.22355091769107105 1105 68-69 -0.2275130152077196 1105 70-71 0.5920632591445667 1105 72-73 0.5621551030872638 1105 74-75 -0.49046930762019514 1105 76 -0.0624321983304128 1106 1 -0.4323449969655968 1106 2 -0.10146885373659131 1106 3 0.11256355242416305 1106 4 -0.058322322080705646 1106 5 -0.3262914229672873 1106 6 -0.10867706499880114 1106 7 -1.4093898417829607 1106 8 0.020077771001076883 1106 9 -0.2801073764867681 1106 10-11 0.3409310613690053 1106 12-13 1.2195477765589544 1106 14-15 -0.11238825068581804 1106 16-17 0.39268533527774707 1106 18-19 0.500031773900524 1106 20-21 0.32034564379679864 1106 22-23 0.307529693706698 1106 24-25 -0.42760850435955433 1106 26-27 0.6249589960760389 1106 28-29 0.34461310612449125 1106 30-31 0.4392205040361503 1106 32-33 -0.2540306987359102 1106 34-35 0.46681313955586745 1106 36-37 0.7899735937410384 1106 38-39 -0.6291086288480159 1106 40-41 0.7853014899882922 1106 42-43 0.5767022638377242 1106 44-45 1.1113598785789947 1106 46-47 0.5485287646236117 1106 48-49 1.1565227740124442 1106 50-51 0.49520069953183565 1106 52-53 0.4211459396722219 1106 54-55 -0.04780582085039242 1106 56-57 0.15786019436104226 1106 58-59 0.7410376114804791 1106 60-61 0.6838928536243714 1106 62-63 0.6493593124322672 1106 64-65 -0.9844349646747048 1106 66-67 0.2073166126963173 1106 68-69 1.1701583161712321 1106 70-71 -0.0976596237582541 1106 72-73 -0.4323551021232106 1106 74-75 -0.3566676592989424 1106 76 -1.003579385674847 1107 1 0.5113783024100158 1107 2 -0.41179857221616345 1107 3 -0.041067732492038544 1107 4 0.17623239366033516 1107 5 0.19170069007904544 1107 6 0.2543966561097477 1107 7 0.5569262936097417 1107 8 -0.1797524404130968 1107 9 -0.4882079351459865 1107 10-11 0.5059683053168627 1107 12-13 -0.3416719627334146 1107 14-15 -0.1953108051824799 1107 16-17 0.3085441368989521 1107 18-19 0.5179691164353031 1107 20-21 0.4837397723979677 1107 22-23 -0.03792161530852667 1107 24-25 0.0889321901305351 1107 26-27 0.03681955035361284 1107 28-29 0.49665604620555825 1107 30-31 0.5858137782173358 1107 32-33 0.7074519390119249 1107 34-35 -0.010114782450926896 1107 36-37 0.826505524949269 1107 38-39 0.1047340702427988 1107 40-41 0.15315393823015455 1107 42-43 0.6799309854939821 1107 44-45 0.3178447071472874 1107 46-47 0.6240025295485765 1107 48-49 0.7456343963149905 1107 50-51 1.0293090356036956 1107 52-53 0.6051200879907626 1107 54-55 0.6967308206218448 1107 56-57 0.7988121058518942 1107 58-59 0.47812546338101924 1107 60-61 1.1070772111662706 1107 62-63 0.7896813630402164 1107 64-65 0.5595731413610068 1107 66-67 -0.1888932679039641 1107 68-69 0.8873918576325011 1107 70-71 0.21587416964738537 1107 72-73 1.0144216532716896 1107 74-75 -0.7837133379055814 1107 76 1.1947994071830834 1108 1 -0.49156287406056975 1108 2 0.08097748557537443 1108 3 -0.04633089038677696 1108 4 0.3293283688925328 1108 5 0.12157685106976146 1108 6 -0.268100763910887 1108 7 -0.12635544013638622 1108 8 -0.48527359624385014 1108 9 -0.048734250935460466 1108 10-11 0.305529708825631 1108 12-13 0.905257861827991 1108 14-15 0.7251742309372347 1108 16-17 -0.11495947507214765 1108 18-19 0.23850575214222403 1108 20-21 0.8820627858138579 1108 22-23 0.06127859108982392 1108 24-25 0.7594688258374447 1108 26-27 -0.365837828387356 1108 28-29 -0.6241901870245812 1108 30-31 0.3317374108282749 1108 32-33 0.6637728884443277 1108 34-35 0.2919234012436078 1108 36-37 0.8796530997686745 1108 38-39 1.0943109536277262 1108 40-41 0.6764511518524543 1108 42-43 -0.14042505165771502 1108 44-45 0.6936445007489382 1108 46-47 0.701763107463961 1108 48-49 0.569989401474956 1108 50-51 0.5882615639834654 1108 52-53 -0.17604606268002243 1108 54-55 -0.2092289317001459 1108 56-57 0.15644884475798193 1108 58-59 0.3019902724625467 1108 60-61 0.8254982637978543 1108 62-63 0.8780714559194678 1108 64-65 0.48098697005037394 1108 66-67 0.6674018817348397 1108 68-69 1.2901524355478813 1108 70-71 0.06551297253695765 1108 72-73 0.00386953768861531 1108 74-75 -0.6460972388343791 1108 76 0.8172968272037195 1109 1 -1.3083116425334786 1109 2 0.1542209390366196 1109 3 0.2027746320399828 1109 4 0.12559489728247542 1109 5 0.2148099337765288 1109 6 -0.270330476122453 1109 7 0.0073754387850542 1109 8 0.3656208813293915 1109 9 0.7100679269229815 1109 10-11 0.4614280754317974 1109 12-13 0.7467927334899116 1109 14-15 1.0500770051140549 1109 16-17 0.8381128609402921 1109 18-19 0.6616714249449132 1109 20-21 0.7515727676650386 1109 22-23 0.15889331756117286 1109 24-25 -0.0272177181086235 1109 26-27 -0.007165284959818052 1109 28-29 -0.47905147818351423 1109 30-31 0.5401506853939964 1109 32-33 0.6204360981928403 1109 34-35 0.2516330201183834 1109 36-37 1.0559559257992674 1109 38-39 1.1070280912994974 1109 40-41 1.2446129356222215 1109 42-43 1.3281126719616765 1109 44-45 1.1007095772332534 1109 46-47 0.9393700558694533 1109 48-49 0.7500775528879728 1109 50-51 0.30436216030184937 1109 52-53 -0.14925062581199455 1109 54-55 -0.19406170328426953 1109 56-57 0.31216572316088786 1109 58-59 0.495378916486402 1109 60-61 0.8004269648608471 1109 62-63 0.5543872453931584 1109 64-65 0.744326352990484 1109 66-67 0.11483512000292251 1109 68-69 0.2600253938055985 1109 70-71 0.6887564259982 1109 72-73 0.6095993795346075 1109 74-75 0.6281962535171957 1109 76 1.1702654556714407 1110 1 -0.9910603614977589 1110 2 -0.7107442811529978 1110 3 -0.2898114510850007 1110 4 -0.19762217329207488 1110 5 0.26936844059633813 1110 6 -0.050116103768068854 1110 7 0.6232875114637224 1110 8 0.7325788237508633 1110 9 0.7341538738992384 1110 10-11 -0.384525832036573 1110 12-13 0.6120550054407943 1110 14-15 0.6668431016063678 1110 16-17 0.2709172771098096 1110 18-19 -0.29179046578954626 1110 20-21 0.8045965070516274 1110 22-23 -0.2551720833349336 1110 24-25 -0.6039932212400458 1110 26-27 0.6541833300363393 1110 28-29 0.3894041002010198 1110 30-31 -0.03905074045446355 1110 32-33 0.5726726504120592 1110 34-35 0.65910139648296 1110 36-37 0.9591294287821626 1110 38-39 0.6516106098033418 1110 40-41 -1.2773441403982773 1110 42-43 0.13924963757793307 1110 44-45 0.7002961801988192 1110 46-47 0.4611786060093124 1110 48-49 1.099955019036443 1110 50-51 -0.19843507711676978 1110 52-53 0.3504151909565749 1110 54-55 -0.40706019327115683 1110 56-57 0.31918357102087924 1110 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2116 38-39 -3.7290840723988765 2116 40-41 -0.6163192356547782 2116 42-43 -0.9916107081065348 2116 44-45 -0.5680096345894086 2116 46-47 -0.752748170731536 2116 48-49 -0.6178648495371917 2116 50-51 -0.9658159374624518 2116 52-53 0.17918817676473253 2116 54-55 0.12926152521136913 2116 56-57 0.05780801190662288 2116 58-59 -2.241358484044099 2116 60-61 -1.2061096020205468 2116 62-63 0.612079553085465 2116 64-65 -1.8134606875551782 2116 66-67 0.10204618480019434 2116 68-69 -0.5236995019882684 2116 70-71 0.9378090974646582 2116 72-73 -0.16379963524371988 2116 74-75 -0.10740435277306659 2116 76 -0.47316008430582457 2117 1 0.6266691701383209 2117 2 0.45419644457508923 2117 3 0.34291016806044894 2117 4 -0.05264313445145774 2117 5 0.16350764490518088 2117 6 0.34859013460611976 2117 7 0.5190647401485791 2117 8 0.49620357735312837 2117 9 -0.5061637362509614 2117 10-11 -0.31700591199437156 2117 12-13 -0.7640936938568004 2117 14-15 0.28654381511868365 2117 16-17 -0.7021941363108795 2117 18-19 -0.8801188480322466 2117 20-21 -0.0797284325635843 2117 22-23 0.4285365149048843 2117 24-25 -1.5011447246132619 2117 26-27 -1.393374903118378 2117 28-29 -0.9954899834770359 2117 30-31 -0.19706456649071669 2117 32-33 -1.46258651835997 2117 34-35 0.7031475943229921 2117 36-37 -0.5033070630693643 2117 38-39 0.07274945319155535 2117 40-41 0.14775094830491042 2117 42-43 0.37529172647546005 2117 44-45 -0.32471839544182046 2117 46-47 -0.2049975787820415 2117 48-49 -0.2447241780539855 2117 50-51 -0.3948730681770485 2117 52-53 0.4246954319404992 2117 54-55 -0.3950387601386396 2117 56-57 0.13222668715402364 2117 58-59 -0.900016124745946 2117 60-61 -0.17607196010445136 2117 62-63 0.156597190144538 2117 64-65 -0.7132846205282846 2117 66-67 -0.31260684173128084 2117 68-69 0.9002425780311611 2117 70-71 -0.22950011266222958 2117 72-73 0.9723487467610212 2117 74-75 0.44118103973497824 2117 76 0.4790589673607428 2118 1 0.35817257567487815 2118 2 0.5222194794489496 2118 3 -0.12307831450262086 2118 4 -0.009964307242249504 2118 5 0.39999511896171214 2118 6 0.48299920824171494 2118 7 0.9781836854553703 2118 8 0.5539076903058557 2118 9 -1.2567264536645055 2118 10-11 -0.8650634407148914 2118 12-13 -0.6915396876011357 2118 14-15 0.7449319585896959 2118 16-17 -2.7785629281492916 2118 18-19 -0.6139169713331327 2118 20-21 -0.034506414965875365 2118 22-23 0.7970068005022384 2118 24-25 0.6938185891345796 2118 26-27 -2.3543647011247586 2118 28-29 -0.5458276139251694 2118 30-31 -0.6630968971639319 2118 32-33 0.0383342919530989 2118 34-35 -0.08671764581849573 2118 36-37 -2.812772408446314 2118 38-39 -0.15724850056330553 2118 40-41 -0.6114352307707733 2118 42-43 0.39799043149460545 2118 44-45 -1.4158932324730102 2118 46-47 0.20492378694042657 2118 48-49 0.4547847231123825 2118 50-51 -0.5392795109260291 2118 52-53 -0.956037708461146 2118 54-55 -0.7792651333152847 2118 56-57 0.19649340059201137 2118 58-59 -0.4423149350005531 2118 60-61 -0.7910709369818747 2118 62-63 -1.3080466170407021 2118 64-65 0.25043837634387955 2118 66-67 0.2918101245641367 2118 68-69 -1.2386974669862312 2118 70-71 0.054109463765023236 2118 72-73 1.1854067139626352 2118 74-75 0.10393060856189607 2118 76 0.9688423576966159 2119 1 0.2643693293292628 2119 2 -0.9691265881683009 2119 3 -0.0834406138479693 2119 4 -0.06412985293321327 2119 5 -0.12094470706286131 2119 6 -0.5593288340863296 2119 7 -0.44357221087679477 2119 8 -0.07697743210469099 2119 9 0.024503929260795587 2119 10-11 -0.14728028225376022 2119 12-13 -0.12768891188595433 2119 14-15 -0.11638092814691703 2119 16-17 -0.6430593858660814 2119 18-19 0.007417438136862131 2119 20-21 -1.0869232385691419 2119 22-23 -0.15519479576715156 2119 24-25 0.7182110203066685 2119 26-27 0.5335377291505594 2119 28-29 0.5059614632876404 2119 30-31 0.9938397004506356 2119 32-33 0.5694077382807876 2119 34-35 0.5245728214041776 2119 36-37 0.25654872867143297 2119 38-39 -1.0360934472048626 2119 40-41 -1.0683897639964997 2119 42-43 0.3843593670160885 2119 44-45 0.18723653167370458 2119 46-47 0.06202180505539445 2119 48-49 -0.06593090777273858 2119 50-51 0.7132987331776377 2119 52-53 -0.17570410609364728 2119 54-55 0.16320639390200142 2119 56-57 -0.8159270101334855 2119 58-59 -0.450879189327523 2119 60-61 0.009619584047626972 2119 62-63 -0.5105845060192706 2119 64-65 -0.07527232224816771 2119 66-67 0.19379200959686216 2119 68-69 0.6495636755121978 2119 70-71 -0.02781144018977244 2119 72-73 0.9278064987352934 2119 74-75 -0.39853553797204455 2119 76 0.06784693127746522 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 2.0 21 2.0 22 7.0 23 12.0 24 35.0 25 52.0 26 104.0 27 213.0 28 372.0 29 601.0 30 1032.0 31 1610.0 32 2552.0 33 3791.0 34 5916.0 35 9178.0 36 16177.0 37 29044.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.381895332390386 23.727015558698728 12.33946251768034 28.55162659123055 2 17.216407355021214 19.660537482319658 39.48939179632249 23.633663366336634 3 16.797736916548796 25.021216407355023 31.77652050919378 26.404526166902404 4 12.954738330975953 20.81046676096181 38.76803394625177 27.466760961810465 5 12.015558698727014 37.68316831683168 37.21074964639321 13.09052333804809 6 26.963224893917964 40.34794908062235 17.718528995756717 14.97029702970297 7 25.5007072135785 29.954738330975957 23.855728429985856 20.68882602545969 8 22.0 37.27015558698727 20.83309759547383 19.896746817538897 9 23.016973125884014 15.042432814710041 23.056577086280054 38.88401697312588 10-11 24.301980198019802 26.699434229137196 27.925742574257427 21.07284299858557 12-13 23.77086280056577 22.582036775106083 27.05940594059406 26.587694483734087 14-15 21.342998585572843 24.931400282885434 23.685289957567186 30.040311173974537 16-17 22.953323903818955 28.473833097595474 25.091937765205092 23.48090523338048 18-19 23.03182461103253 27.32107496463932 25.61951909476662 24.027581329561528 20-21 21.577793493635077 26.64568599717115 25.547383309759546 26.229137199434227 22-23 22.32885431400283 27.862093352192364 25.93493635077794 23.874115983026876 24-25 23.02192362093352 28.253889674681755 25.973833097595474 22.75035360678925 26-27 21.991513437057993 28.56789250353607 26.887553041018386 22.553041018387553 28-29 21.62942008486563 26.364214992927863 28.693776520509196 23.31258840169731 30-31 21.1994342291372 27.75035360678925 27.357142857142858 23.693069306930692 32-33 20.78005657708628 27.694483734087694 29.362800565770865 22.16265912305516 34-35 22.444837340876944 28.3005657708628 27.874115983026876 21.38048090523338 36-37 22.2970297029703 28.71145685997171 25.899575671852897 23.091937765205092 38-39 21.036067892503535 27.143564356435647 28.016973125884014 23.803394625176804 40-41 20.974540311173975 25.718528995756717 29.444837340876944 23.862093352192364 42-43 22.897454031117398 25.296322489391798 28.992927864214995 22.813295615275813 44-45 19.997878359264497 26.901697312588404 29.18104667609618 23.91937765205092 46-47 22.19236209335219 25.28076379066478 28.691654879773694 23.835219236209333 48-49 21.46746817538897 25.17114568599717 28.444837340876944 24.91654879773692 50-51 19.666195190947665 25.939179632248937 29.414427157001416 24.980198019801982 52-53 19.577086280056577 25.76732673267327 31.491513437057993 23.164073550212162 54-55 18.814002828854314 26.074257425742577 30.12022630834512 24.991513437057993 56-57 20.256011315417254 25.70721357850071 28.579207920792076 25.45756718528996 58-59 19.18953323903819 26.52970297029703 29.97878359264498 24.301980198019802 60-61 20.56789250353607 27.78712871287129 27.501414427157002 24.143564356435643 62-63 18.56223479490806 29.466053748231968 27.062234794908065 24.90947666195191 64-65 19.002121640735503 30.403111739745402 25.543847241867045 25.050919377652054 66-67 18.754596888260256 30.702263083451204 25.41371994342291 25.12942008486563 68-69 18.847949080622346 30.690240452616692 25.635785007072137 24.826025459688825 70-71 19.78854314002829 29.994342291371996 24.914427157001416 25.302687411598303 72-73 19.248231966053748 28.493635077793495 25.478783592644977 26.77934936350778 74-75 19.086987270155586 29.0997171145686 25.820367751060818 25.992927864214995 76 19.486562942008486 28.7029702970297 26.11598302687412 25.69448373408769 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 1.5 15 2.0 16 3.0 17 4.0 18 5.5 19 7.0 20 13.5 21 20.0 22 28.0 23 37.5 24 57.0 25 75.0 26 122.5 27 221.5 28 315.5 29 358.0 30 430.0 31 599.5 32 878.0 33 1059.0 34 1233.0 35 1614.5 36 1975.5 37 2129.0 38 2593.0 39 3735.5 40 4784.5 41 5075.5 42 4996.0 43 5059.5 44 5141.0 45 5175.0 46 5191.0 47 5199.0 48 4941.5 49 4381.5 50 4087.0 51 3712.0 52 3002.0 53 2417.0 54 2167.0 55 2117.5 56 1727.5 57 1205.0 58 1023.0 59 857.5 60 581.5 61 402.0 62 333.0 63 305.0 64 251.0 65 176.0 66 113.0 67 99.0 68 93.0 69 65.5 70 28.5 71 13.0 72 9.0 73 3.0 74 0.5 75 0.0 76 0.0 77 0.5 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 70700.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.322489391796324 #Duplication Level Percentage of deduplicated Percentage of total 1 57.29681848379715 21.957567185289957 2 15.04023030929357 11.527581329561528 3 9.016756477448881 10.366336633663366 4 5.4624640141728795 8.373408769448373 5 3.5321473388942204 6.7680339462517685 6 2.3400014763416253 5.38048090523338 7 1.5280135823429541 4.099009900990099 8 1.262272089761571 3.8698727015558694 9 0.9116409537166901 3.144271570014144 >10 3.5801284417214143 20.816124469589816 >50 0.011072562190890973 0.25035360678925034 >100 0.011072562190890973 0.7157001414427158 >500 0.0036908540636303245 0.7553041018387553 >1k 0.0036908540636303245 1.9759547383309761 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT 1397 1.9759547383309761 No Hit ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT 534 0.7553041018387553 TruSeq Adapter, Index 12 (95% over 23bp) CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC 218 0.30834512022630833 No Hit ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 171 0.24186704384724184 No Hit TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG 117 0.16548797736916548 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.002828854314002829 0.0 2 0.0 0.0 0.0 0.002828854314002829 0.0 3 0.0 0.0 0.0 0.004243281471004243 0.0 4 0.0 0.0 0.0 0.02545968882602546 0.0 5 0.0 0.0 0.0 0.028288543140028287 0.0 6 0.0 0.0 0.0 0.0297029702970297 0.0 7 0.0 0.0 0.0 0.033946251768033946 0.0 8 0.0 0.0 0.0 0.04384724186704385 0.0 9 0.0 0.0 0.0 0.10608203677510608 0.0 10 0.0 0.0 0.0 0.12871287128712872 0.0 11 0.0 0.0 0.0 0.14992927864214992 0.0 12 0.0 0.0 0.0 0.16831683168316833 0.0 13 0.0 0.0 0.0 0.17114568599717114 0.0 14 0.0 0.0 0.0 0.18246110325318246 0.0 15 0.0 0.0 0.0 0.18953323903818953 0.0 16 0.0 0.0 0.0 0.19519094766619519 0.0 17 0.0 0.0 0.0 0.20226308345120225 0.0 18 0.0 0.0 0.0 0.22206506364922207 0.0 19 0.0 0.0 0.0 0.24611032531824611 0.0 20 0.0 0.0 0.0 0.26308345120226306 0.0 21 0.0 0.0 0.0 0.26874115983026875 0.0 22 0.0 0.0 0.0 0.28005657708628007 0.0 23 0.0 0.0 0.0 0.3055162659123055 0.0 24 0.0 0.0 0.0 0.31541725601131543 0.0 25 0.0 0.0 0.0 0.32107496463932106 0.0 26 0.0 0.0 0.0 0.33097595473833097 0.0 27 0.0 0.0 0.0 0.347949080622348 0.0 28 0.0 0.0 0.0 0.3734087694483734 0.0 29 0.0 0.0 0.0 0.413012729844413 0.0 30 0.0 0.0 0.0 0.4512022630834512 0.0 31 0.0 0.0 0.0 0.5021216407355021 0.0 32 0.0 0.0 0.0 0.5346534653465347 0.0 33 0.0 0.0 0.0 0.5869872701555869 0.0 34 0.0 0.0 0.0 0.6633663366336634 0.0 35 0.0 0.0 0.0 0.7694483734087695 0.0 36 0.0 0.0 0.0 0.9335219236209336 0.0 37 0.0 0.0 0.0 1.2404526166902405 0.0 38 0.0 0.0 0.0 1.7114568599717115 0.0 39 0.0 0.0 0.0 2.2093352192362095 0.0 40 0.0 0.0 0.0 2.835926449787836 0.0 41 0.0 0.0 0.0 3.4257425742574257 0.0 42 0.0 0.0 0.0 4.124469589816124 0.0 43 0.0 0.0 0.0 4.773691654879774 0.0 44 0.0 0.0 0.0 5.463932107496464 0.0 45 0.0 0.0 0.0 6.202263083451203 0.0 46 0.0 0.0 0.0 6.807637906647807 0.0 47 0.0 0.0 0.0 7.557284299858558 0.0 48 0.0 0.0 0.0 8.185289957567186 0.0 49 0.0 0.0 0.0 8.88967468175389 0.0 50 0.0 0.0 0.0 9.492220650636492 0.0 51 0.0 0.0 0.0 10.134370579915135 0.0 52 0.0 0.0 0.0 10.783592644978784 0.0 53 0.0 0.0 0.0 11.396039603960396 0.0 54 0.0 0.0 0.0 11.946251768033946 0.0 55 0.0 0.0 0.0 12.596888260254596 0.0 56 0.0 0.0 0.0 13.216407355021216 0.0 57 0.0 0.0 0.0 13.78925035360679 0.0 58 0.0 0.0 0.0 14.34087694483734 0.0 59 0.0 0.0 0.0 14.919377652050919 0.0 60 0.0 0.0 0.0 15.407355021216407 0.0 61 0.0 0.0 0.0 15.94059405940594 0.0 62 0.0 0.0 0.0 16.44130127298444 0.0 63 0.0 0.0 0.0 16.912305516265913 0.0 64 0.0 0.0 0.0 17.468175388967467 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCAGCTG 15 0.0022162544 70.00001 21 GCGAGAC 20 7.8773446E-5 70.0 11 ATAGCAT 20 7.8773446E-5 70.0 3 CGCTGGA 20 7.8773446E-5 70.0 17 GAGACGC 20 7.8773446E-5 70.0 13 GATAGCA 20 7.8773446E-5 70.0 2 AGCCGGA 25 2.3756683E-4 55.999996 23 GGAGCAC 25 2.3756683E-4 55.999996 27 GCAGTAT 25 2.3756683E-4 55.999996 42 ATGTCGC 25 2.3756683E-4 55.999996 37 TTGCGAG 25 2.3756683E-4 55.999996 9 ACGCTGG 25 2.3756683E-4 55.999996 16 GAGCCGG 25 2.3756683E-4 55.999996 22 GAGCACC 25 2.3756683E-4 55.999996 28 GACGCTG 25 2.3756683E-4 55.999996 15 TATGTCG 25 2.3756683E-4 55.999996 36 CGATAGC 25 2.3756683E-4 55.999996 1 AAATAAC 20 0.0069252164 52.5 49 GGTTTAC 20 0.0069252164 52.5 50 GCCCTCC 20 0.0069252164 52.5 23 >>END_MODULE