FastQCFastQC Report
Wed 25 May 2016
SRR1780645_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780645_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGT11.2987012987012987No Hit
CTTTGGGTGCTAATGGTGGAGTTAAAGACTTTTTCTCTGATTTGTCCTTG11.2987012987012987No Hit
GACTCGGACAGGGCGCCCTTGTTGGGCACGGCGATGCGCAGCACGCGTCG11.2987012987012987No Hit
GTGTGTTGGTTAGTAGGCCTAGTATGAGGAGCGTTATGGAGTGGAAGTGA11.2987012987012987No Hit
CTCATGACGACCATCGCGTTCGCCGTGGCCGCGCTCAAGCTGGCCCAGCA11.2987012987012987No Hit
CTGCATGTGCCATTAAGATATATAGGATTTAGCCTATAATTTAACGTTGA11.2987012987012987No Hit
GTTGAGGGATAGGAGGAGAATGGGGGGTAGGGGGAGGAACATGAGGGGGG11.2987012987012987No Hit
GCCCTCGGGGCTGTCCGTGGGCGCGGACGGCGCGCTGTGGGTCGCGGACT11.2987012987012987No Hit
GGAGAGATTTGGTATATGATTGAGATGGGGGCTAGTTTTTGTCATGTGAG11.2987012987012987No Hit
TCATGACCCTTGGCCATAATATGCTTTATCTCCACACTTGCAGAGACCAA11.2987012987012987No Hit
AATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTAC11.2987012987012987No Hit
TATGAGGTGTGAGCGATATACTAGTATTCCTAGAAGTGAGATGGTAAATG11.2987012987012987No Hit
GTAAAAGGGTAGCTTACTGGTTGTCCTCCGATTCAGGTTAGAATGAGGAG11.2987012987012987No Hit
CGCGTGCGCTTCACCTCGGAGCGACGGTGTTTGGCCTGTCTCTTATACAC11.2987012987012987No Hit
CTACTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACCTGTCTCTTATAC11.2987012987012987No Hit
GTCGCGGGCATCACGGTGCTCTACACCCTGTTCGGCGGCTTCCTCGGCGC11.2987012987012987No Hit
GAGCGCAGCCGCGCCCTGGCCGCGTGGGCGGACGTGGTGCCTGTCTCTTA11.2987012987012987No Hit
GTTAGGACCCAATCACTTCTTAAAGGCGCTATTTCTCAAGACTGCCACAT11.2987012987012987No Hit
CGCGTGGTGAGGCAGAAGTCCTCCCCGAGCTGTCTCTTATACACATCTGA11.2987012987012987No Hit
GGTTTAGGTTATGTACGTAGTCTAGGCCATATGTGTTGGAGATTGAGACT11.2987012987012987No Hit
ACCGGGTGCCGGTCTACCAGCGGGCCTACGCCTGGACCTGTCTCTTATAC11.2987012987012987No Hit
CTTATACACATCTGACGCTGCCGACGCCAGTCAGTGTCTCTTATACACAT11.2987012987012987No Hit
CCATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCC11.2987012987012987No Hit
GGAGTGGACCTCGGGTCGCTCCCGATCCTACGCCCCCGCGGGGCGTGGAC11.2987012987012987No Hit
GTGCTGATCGTGGCGGGGGGCGGGGAGGCCGGGCTCGGCCCCGACGGCGC11.2987012987012987No Hit
GGGTGTGGCCTCCATCGCCTGGTTAGGGGTGGGGCAGAGTGGGACTGCCC11.2987012987012987No Hit
GTAAATAAGGGGTCGTAAGCCTCTGTTGTCAGATTCACAATCTGATGTTT11.2987012987012987No Hit
ATCCGCGGGAGCTGCTCGAGGAGCTCGTGGCCCTGTGCCTGTCTCTTATA11.2987012987012987No Hit
CAATTAAATTACCACACACCTAATAGGATGGCATAAAACCTAAACACCGA11.2987012987012987No Hit
GTGTCGTAGTCAATCTGTGCGCTCTGCGGGGAGAGGCCCCCTTATCAGGG11.2987012987012987No Hit
GTAATAAACTTCGCCTTAATTTTAATAATGAACACCCTCCTAGCCTTACT11.2987012987012987No Hit
AACAGCCTTTAACCTCTCCACCCTTATCACAACACAAGAACACCTCTGAT11.2987012987012987No Hit
GTACCACTCGAAGAACAGGTTGCCGGGCTCGGAGCGCACGTCCGCGGTGC11.2987012987012987No Hit
GGCTTAGCTTAATTAAAGTGGCTGATTTGCGTTCAGTTGATGCAGAGTGG11.2987012987012987No Hit
GGAGGAGCCCATTCCACGCCTGGGGGCTGCTGGCCATCCTTGCCATGCCT11.2987012987012987No Hit
ACGTGGAGCGGCTCAGCCCGACCAGACGGCACGCCATCCGTTCGCTCACC11.2987012987012987No Hit
CACCACACACATCACACACACCACACATACACACCACACACACATCACAC11.2987012987012987No Hit
CCTTAGTACTGATGGCTGGTTCCTCCTCTTGACTCCTGTACCTTGATTTG11.2987012987012987No Hit
CTTCAGGAACCGCCCTGTGGTGTCAGAACTTTGAGGCCGCCCGGCCTGGG11.2987012987012987No Hit
CCCCATCACCGACCCCGTCAAAGACGGGGTTCGGGATGGTTCGGGCGCTG11.2987012987012987No Hit
CCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAACATAA11.2987012987012987No Hit
ATAGATATAGGGGTGATAATTGGCAGGTCAGCGGTTTCAACTTACTCAGG11.2987012987012987No Hit
ATGTATGGGATGGCGGATAGTAAGTTTGTAATTACGGCGGCCCCTCAGAA11.2987012987012987No Hit
GGGGCACCGACGGGTGGCCCGGCAGCGAAAAAGCACGCGCATCGTTGCAG11.2987012987012987No Hit
GGCTGTGCAACAACGAATACAGAGATCTACAGGAAGGGGGTTTTGGAGGC11.2987012987012987No Hit
GTAGGGCTGAGACTGGGGTGGGGCCTTCTATGGCTGAGGGGAGTCAGGGG11.2987012987012987No Hit
ATGCAGAGGACCCGGAGCACGGCCGGGCCCGGGATTCTCCGGCGCAGTCT11.2987012987012987No Hit
TCTCCACACTCATCGCCCTTACCACGCTACTCCTACCCTGTCTCTTATAC11.2987012987012987No Hit
GTAATAGATAGGGCTCAGGCGTTTGTGTATGATATGTTTGCGGTTTCGAT11.2987012987012987No Hit
TCATAAGTATGTCAAATCGGGAACGTCCAGGGGATGGACAGGCGCGCCGC11.2987012987012987No Hit
ACCTTGCACGAGCTGGCCCAATCGGGGGTCCGCGTCCGTGCGCTCGACCT11.2987012987012987No Hit
ATTCTCCGATCCGTCCCTAACAAGCTAGCAGGCGCCCTTCCCCTATTACT11.2987012987012987No Hit
ATACACATCTGACGCAGCCGACGAACTTCTAGGTGTAGATCTCGGGGGTT11.2987012987012987No Hit
ATATGGATGCCCCCCACCCTACCACACATTCGAAGAACCCGTATACATAA11.2987012987012987No Hit
CCGCCAGGCCGTCCGGGTGGTCCACGCGCAGCCCGTCCACGAGCCCTTCG11.2987012987012987No Hit
CCCATGAAAGAACAGAGAATCGTTTAAATTAGAATCTTAGCTTTGGGTGC11.2987012987012987No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT11.2987012987012987No Hit
GTACTCGTAGGTTCAGTACCATTGGTGGCCAATTGATTTGATGGTAAGGG11.2987012987012987No Hit
CACCGAGGTCGAGCAGCGTCCGCCCGGCCCAGGGCCTGTCTCTTATACAC11.2987012987012987No Hit
GGTTATAGTAGTGTGCATGGTTATTACTTTTATTTGGAGTTGCACCAAAA11.2987012987012987No Hit
CCGTCAGCGCCGGGCTCGCGCTGCCCACCGTCCTGGCCTGTCTGTCCCTG11.2987012987012987No Hit
ACCCATGCGGCTGTGCCGTCGAACTTCGCCGTGGGCCGTTCCTTCATCGT11.2987012987012987No Hit
ATCATCGAGCGCGGCTGGATGCCGGACCAGCGGGTCACCGTGGGCACGGG11.2987012987012987No Hit
GCCAGGCGATCTACCTGCTGGGCGAGACCGCGGACGAGCTGGACCTGTCT11.2987012987012987No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG11.2987012987012987No Hit
CAGTGAGGTGGTTTGGCTGTGTTTACACCCAAATCTCATCTTGATTGTAG11.2987012987012987No Hit
GCTTGGGGTCCTAAAATGGTTGCAGCGCCAGGGCACCTGAACCAGCCAAA11.2987012987012987No Hit
ACACCATGGTGCTGCCCGCCGCGGGGCCCCTGCCTGTCTCTTATACACAT11.2987012987012987No Hit
TGAACCGGTACAATCTTTAGCAAAGAAGTGGTGGGAATGGATCAGCGTCA11.2987012987012987No Hit
TAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGGGTTAATGTCAT11.2987012987012987No Hit
GCACATACTTAGTCTTGCTCTGAAGAAGGCGCGGGCACAGATGGGCAGGA11.2987012987012987No Hit
CATCTGACGCTGCCGACGAATGACCATGAGTAGATCTCGCTGGTGGGGGG11.2987012987012987No Hit
TGCCGGGCTGCGCGCGCTGCTCAAGCTCGTGCCCCAGAGCGCCACCGTCC11.2987012987012987No Hit
GCGTTGAGCTGCGCCTGCAGCGCGTACTCCACGTACTGTCTCTTATACAC11.2987012987012987No Hit
GGCCGGCGCTGCCGGCGCCGGGAAGCCCGACGACGACGGCGCCGTGGCCT11.2987012987012987No Hit
GCGGATGGCGGCCCTGCGCCGCCCCGTGCGCCCGTTCATGCTGCGCCTGT11.2987012987012987No Hit
CTTCACCTCCAAAAGATTGCAAAAAGTCTCCAGCTGAGCTTAGAATCTGT11.2987012987012987No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAAT50.044.029
CGTTTAA50.044.021
GAGAATC50.044.015
AATCTTA50.044.033
TAAATTA50.044.025
AGCTTTG50.044.039
CATGAAA50.044.03
ATCTTAG50.044.034
ACAGAGA50.044.012
AAATTAG50.044.026
ATTAGAA50.044.028
TAGCTTT50.044.038
AATTAGA50.044.027
CCATGAA50.044.02
ATGAAAG50.044.04
CTTAGCT50.044.036
CCCATGA50.044.01
AGAATCT50.044.031
AGAATCG50.044.016
AGAACAG50.044.09
Produced by FastQC (version 0.11.5)