FastQCFastQC Report
Wed 25 May 2016
SRR1780645_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780645_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTTCTGCTTG22.5974025974025974Illumina PCR Primer Index 3 (96% over 28bp)
GACCTGCCCCGCGGCCAGGTCACGGGCCGGGACGCTCGCGCTATGGCCGT11.2987012987012987No Hit
CTTATACACATCCCGAGCCCACGAGACACCGGCTAATCTCGTTTTCCGTC11.2987012987012987No Hit
CAATGAACAAGATATTCGAAAAATAGGAGGACTACTCAAAACCATACCTC11.2987012987012987No Hit
GTTCAACCTGTTCCTGCTCCGGCCTCCACTATAGTAGCTGTCTCTTATAC11.2987012987012987No Hit
CGCAAGCAACCGCATCCATAATCCTTCTAATAGCTATCCTCTTCAACAAT11.2987012987012987No Hit
TCTCACCAGACCTCCTAGGCGACCCAGACAATTATACCCTAGCCAACCCC11.2987012987012987No Hit
GCCCTCGGCTTACTTCTCTTCATTCTCTCCTTAATGACATTAACACTATT11.2987012987012987No Hit
GCCCTGGGCCACGTCCGTCAGCGAGGCGCCGAGGAAGCCGCCGAACAGGG11.2987012987012987No Hit
ATCTCCGACCACGAGACCCAACACGATCTCGTATGCCGTCTTCTGCTTGA11.2987012987012987TruSeq Adapter, Index 1 (96% over 31bp)
AGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAA11.2987012987012987No Hit
GTCCAGGCGTAGGCCCGCTGGTAGACCGGCACCCGGTCTGTCTCTTATAC11.2987012987012987No Hit
CATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATAC11.2987012987012987No Hit
GGTCTACGGAGGCTCCAGGGTGGGAGTAGTTCCCTGCTAAGGGAGGGTAG11.2987012987012987No Hit
GCTCCTGGATGAGGACCTCCTGCTGGGCCAGCTTGAGCGCGGCCACGGCG11.2987012987012987No Hit
CAATCACACTACTCCCCATATCTAACAACGTAAAAATAAAATGACAGTTT11.2987012987012987No Hit
CATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCTTCTGCT11.2987012987012987TruSeq Adapter, Index 7 (96% over 26bp)
GGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGTCTGTTAGTAGTATGG11.2987012987012987No Hit
TCTTACAGCTGCGCTCCACTACCACAGTACCAAATACTGTATTAGTCCAT11.2987012987012987No Hit
GAGTAGTAAGTTACAATATGGGAGATTATTCCGAAGCCTGGTAGGATAAG11.2987012987012987No Hit
ATGGGGAGGCAGTCTGAGCTCTCTGCCTGTCCCTCTTCCGGCCTCAGCCT11.2987012987012987No Hit
CCTCACTACCCTCCACCAGAGGATTGGGGCTAATACAGGAGGAAAAGAGG11.2987012987012987No Hit
GCAACGGACCCTGGGAGTCGGCCATCATGCTGGCCCCGGAGGCCGTTATC11.2987012987012987No Hit
ATCCAAGCCTCACCCCACTACTAGGCCTCCTCCTAGCAGCAGCAGGCAAA11.2987012987012987No Hit
TGACAGGCAAGTGCCACCACGCCTGGCTAATTTCTGTCTCTTATACACAT11.2987012987012987No Hit
CCTCAAGCGCAAGTTGCGGGTGAGCGAACGGATGGCGTGCCGTCTGGTCG11.2987012987012987No Hit
GCACCACGTCCGCCCACGCGGCCAGGGCGCGGCTGCGCTCCTGTCTCTTA11.2987012987012987No Hit
GCACAGGGCCACGAGCTCCTCGAGCAGCTCCCGCGGATCTGTCTCTTATA11.2987012987012987No Hit
GCGATGGTCTCCACCGTGCCCACGGTGACCCGCTGGTCCGGCATCCAGCC11.2987012987012987No Hit
CCACCGCACCCCGAGGAGACCGACGCGTGCTGCGCATCGCCGTGCCCAAC11.2987012987012987No Hit
TCATTATAGCTACTCTCATAACCCTCAACACCCACTCCCTCTTAGCCAAT11.2987012987012987No Hit
TACGTGGAGTACGCGCTGCAGGCGCAGCTCAACGCCTGTCTCTTATACAC11.2987012987012987No Hit
CTTATACACATCTCCGAGCCACGAGACCGGTTCCCATCTCGTATGCCGTC11.2987012987012987No Hit
GATTTAGCCTATAATTTAACTTTGACAAAGTTATGAAATGGTTTTTCTAA11.2987012987012987No Hit
CTTATACACATCTCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC11.2987012987012987No Hit
CAATGTGGCAGTCTTGAGAAATAGCGCCTTTAAGAAGTGATTGGGTCCTA11.2987012987012987No Hit
CCCACTAGGATACCAACAAACCTACCCATCTTTAACAGTACATAGTACAT11.2987012987012987No Hit
TCTTATACACATCTCCGAGCCCAGAGACCGGTTCCCATCTCGTATGCCGT11.2987012987012987No Hit
GGACAGGGCCTCGATAAGACCAAGGCAGAGGCAAGAGCCAAGGCATGGCA11.2987012987012987No Hit
ATCTCCGAGCCACGAGACTAACAACAATCTCGTATGCCGTCTTCTGCTTG11.2987012987012987RNA PCR Primer, Index 36 (100% over 29bp)
CCGCGCTGCTGCTCATCCGCTCCGGCCAGGCCGACGTCGTGATCTGCGGC11.2987012987012987No Hit
ATTAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCAA11.2987012987012987No Hit
CACCGCGGACGTGCGCTCCGAGCCCGGCAACCTGTTCTTCGAGTGGTACC11.2987012987012987No Hit
GCGCAGCATGAACGGGCGCACGCGGCGGCGCAGGGCCGCCATCCGCCTGT11.2987012987012987No Hit
GTATGTTGGGAGAACCACTGCTCTCTTCAAAGCTGTCAGACAGGGACATT11.2987012987012987No Hit
ATCTCCGAGTCCACGAGACGATCCAAAATCTCGTATGCCGTCTTCTGCTT11.2987012987012987Illumina PCR Primer Index 6 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGATTTATGCATCTCGTATGCCGTCT11.2987012987012987TruSeq Adapter, Index 3 (95% over 22bp)
ATTCTAAGCTCAGCTGGAGACTTGTTGCAATCTTTTGGAGGAGAAGCTGT11.2987012987012987No Hit
CTTATACACATCTCCGAGCCCCGAGACTAACAACAATCTCGTATGCCGTC11.2987012987012987No Hit
CCTGACCCCTCTCCTTCATAAATTATTCAGCTTCCTACACTATTAAAGTT11.2987012987012987No Hit
GCTCGAGGTAGGTGAACCCGGCGGCGTGGGCGTCCACGAACTGTCTCTTA11.2987012987012987No Hit
CCTCGGCGCCGTCCTCGACCTTCCACGAGGCCAGGGTGCCGGTGACCGGC11.2987012987012987No Hit
GCCAAACACCGTCGCTCCGAGGTGAAGCGCACGCGCTGTCTCTTATACAC11.2987012987012987No Hit
GTGTAGTGTGTGTGTGGTGTGTGATGTGTGTGTGGTGTGTATGTGTGGTG11.2987012987012987No Hit
GATCTCCTCGACCTTCGCCGATGACTCGCCCGAGCCCACGAGACCCAACA11.2987012987012987No Hit
GCCCTGGGCCGGGCGGACGCTGCTCGACCTCGGTGCTGTCTCTTATACAC11.2987012987012987No Hit
GGACAGACAGGCCAGGACGGTGGGCAGCGCGAGCCCGGCGCTGACGGCTG11.2987012987012987No Hit
TCTTATACACATCTCCGACCACGAGACCGGTTCCCATCTCGTATGCCGTC11.2987012987012987No Hit
CTTTGTAGCCTTCATCAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAG11.2987012987012987No Hit
CTCGGGGAGGACTTCTGCCTCACCACGCGCTGTCTCTTATACACATCTCC11.2987012987012987No Hit
ACGCCCAGCGGGTGCTGCAGGCGCGCGCGGTCCGCCTCCCCGTCCACGTG11.2987012987012987No Hit
CGCCCGAACCATCCCGAACCCCGTCTTTGACGGGGTCGGTGATGGGGCTG11.2987012987012987No Hit
GTGGTGGTACCATTTTGCATTCTCATCAGCAATGAATGAGAGTTGTTGCA11.2987012987012987No Hit
CTAATACACCAGTCTTGTAAACCGAAGATGAAAACCTTTTTCCAAGGACA11.2987012987012987No Hit
TCCGCGAAGGGCTCGTGGACGGGCTGCGCGTGGACCACCCGGACGGCCTG11.2987012987012987No Hit
CTTATACACATCTCCGAGCCCACGAACACCGGCTAATCTCGTATGCCGTC11.2987012987012987No Hit
CATCGGGCGAGGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAA11.2987012987012987No Hit
TCTCAACTTTTAAAGGATAACAGCTATCCATTGGTCTTAGGCCCCAAAAA11.2987012987012987No Hit
ACATAAAACCCTCATTCACACGAGAAAACACCCTCATGTTCATACACCTA11.2987012987012987No Hit
GGCACCAACGTCACCGGGTTCGCCCGCTTCCGCGTCGGCAACTGACCGCC11.2987012987012987No Hit
GTCCAGGTCCAGGTCGAGCGCACGGACGCGGACCCCCGATTGGGCCAGCT11.2987012987012987No Hit
CGGCGACGTCGAGGCCGAGCTCGGCCATCGCGCCCACCGCCACGATCAGC11.2987012987012987No Hit
ATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCTT11.2987012987012987TruSeq Adapter, Index 7 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC11.2987012987012987No Hit
ATGGTTGCATTCTTTTGAGTTTCTGATGAGCCTCTCCAAAGGAGGCAATC11.2987012987012987No Hit
GTCCAGCTCGTCCGCGGTCTCGCCCAGCAGGTAGATCGCCTGGCCTGTCT11.2987012987012987No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC50.044.016
ATGGGAT50.044.031
GTATGCC50.044.041
ATCTCGT50.044.036
ATCTCGA50.044.010
CACATCT50.044.07
GCCCACG50.044.017
TATACAC50.044.03
TGCCGTC50.044.044
CCACGAG50.044.019
TATGCCG50.044.042
CATCTCG50.044.09
GGATCTC50.044.034
GAGCCCA50.044.015
CACGAGA50.044.020
CCCACGA50.044.018
ACATCTC50.044.08
TTATACA50.044.02
TACACAT50.044.05
ACACATC50.044.06