FastQCFastQC Report
Wed 25 May 2016
SRR1780644_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780644_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99366
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAATGC150.002219922869.9919522
CTGTCAG150.002219922869.9919514
GCGACTG150.002219922869.9919567
AGAACTT150.002219922869.9919512
ATCGTCA150.002219922869.9919531
GCACATC150.002219922869.9919515
CAGATGA150.002219922869.9919567
TCGTCAC150.002219922869.9919532
CCAAGGG150.002219922869.9919531
CATCGTT150.002219922869.9919532
TACTAGC150.002219922869.9919570
TATCGTC150.002219922869.9919530
CGTCACA150.002219922869.9919533
GTTACTA252.3817364E-455.9935668
AGGTAAC200.00693660652.493966
TCGTTGT200.00693660652.4939634
ATTAGAG200.00693660652.493963
CGTATAC200.00693660652.4939624
TTTAACG200.00693660652.4939634
TTAGAGA200.00693660652.4939646
Produced by FastQC (version 0.11.5)