##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780644_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 99366 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.72518768995431 34.0 31.0 34.0 12.0 34.0 2 31.262564659944044 34.0 32.0 34.0 23.0 34.0 3 31.323289656421714 34.0 33.0 34.0 23.0 34.0 4 31.731648652456574 34.0 34.0 34.0 23.0 34.0 5 31.6909405631705 34.0 34.0 34.0 23.0 34.0 6 34.18901837650706 38.0 34.0 38.0 23.0 38.0 7 33.80968339270978 37.0 34.0 38.0 22.0 38.0 8 32.52105347905722 37.0 31.0 38.0 11.0 38.0 9 33.48623271541574 37.0 34.0 38.0 21.0 38.0 10-11 33.87261739427973 37.0 34.0 38.0 22.0 38.0 12-13 34.27315178229978 38.0 34.5 38.0 24.0 38.0 14-15 33.87265764949782 37.0 34.0 38.0 22.0 38.0 16-17 33.74178793551114 37.0 34.0 38.0 21.0 38.0 18-19 33.718505323752595 37.0 34.0 38.0 22.0 38.0 20-21 33.7847553489121 37.0 34.0 38.0 21.5 38.0 22-23 33.78425719058833 37.0 34.0 38.0 21.5 38.0 24-25 34.2034347764829 38.0 34.5 38.0 23.5 38.0 26-27 34.29243906366362 38.0 34.5 38.0 23.5 38.0 28-29 34.40115834390033 38.0 35.5 38.0 24.0 38.0 30-31 34.224427872713 38.0 35.0 38.0 23.0 38.0 32-33 33.989166314433504 38.0 34.0 38.0 22.0 38.0 34-35 33.83597005011775 37.0 34.0 38.0 22.0 38.0 36-37 33.84063965541533 37.0 34.0 38.0 22.0 38.0 38-39 34.3480768069561 38.0 35.0 38.0 24.0 38.0 40-41 33.84364873296701 37.5 34.0 38.0 22.0 38.0 42-43 34.17228730149146 38.0 34.5 38.0 23.5 38.0 44-45 33.23323370166858 37.0 34.0 38.0 21.0 38.0 46-47 33.93499285469879 37.0 34.0 38.0 23.0 38.0 48-49 33.641683271944125 37.0 34.0 38.0 21.5 38.0 50-51 33.62766439224684 37.0 34.0 38.0 22.0 38.0 52-53 33.63738099551155 37.0 34.0 38.0 21.5 38.0 54-55 33.33269428174627 37.0 34.0 38.0 21.0 38.0 56-57 33.40810740092184 37.0 33.5 38.0 21.5 38.0 58-59 33.31648652456575 37.0 33.0 38.0 21.5 38.0 60-61 33.3199937604412 37.0 33.5 38.0 21.0 38.0 62-63 33.42213634442365 37.0 33.5 38.0 21.5 38.0 64-65 33.41687297465934 37.0 33.5 38.0 21.0 38.0 66-67 33.38030614093352 37.0 33.5 38.0 21.0 38.0 68-69 32.95446631644627 37.0 32.0 38.0 16.0 38.0 70-71 32.799730290038845 37.0 32.0 38.0 19.5 38.0 72-73 32.89198015417749 37.0 32.0 38.0 19.5 38.0 74-75 32.52238693315621 37.0 31.0 38.0 15.0 38.0 76 31.790209930962302 36.0 30.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.2656348896585534 1101 2 -0.9859805782104551 1101 3 -0.505809155760474 1101 4 -2.5274676117139308 1101 5 -1.0755481015228128 1101 6 -2.183348653135326 1101 7 -1.8052146696091356 1101 8 -0.5288519278491712 1101 9 -0.29127644615521575 1101 10-11 -1.3660046309323874 1101 12-13 -2.0844578180313533 1101 14-15 -0.6940878629642384 1101 16-17 -2.7894064070946456 1101 18-19 0.017076048871999205 1101 20-21 -1.3503658753248828 1101 22-23 -0.7969856366053847 1101 24-25 -1.4621261262537857 1101 26-27 -1.2895861796593735 1101 28-29 -0.6647886299507064 1101 30-31 -1.8198810351489385 1101 32-33 -0.3549294068439224 1101 34-35 -0.5372783985395202 1101 36-37 -0.018417466308491726 1101 38-39 -1.332528089113147 1101 40-41 -0.031896162578028964 1101 42-43 -0.7414619831543732 1101 44-45 -0.3960827267797882 1101 46-47 -0.05791790717729128 1101 48-49 -1.383237263453843 1101 50-51 -1.9407522982523417 1101 52-53 -0.6944041934556395 1101 54-55 -1.8851916473844952 1101 56-57 -1.3912589285172885 1101 58-59 -1.2273153123156533 1101 60-61 -0.24355505102902697 1101 62-63 -2.188625061319115 1101 64-65 -1.8675765905392545 1101 66-67 -1.526493346064914 1101 68-69 -0.30148813830904686 1101 70-71 -0.34129128018651755 1101 72-73 -1.1061544576195566 1101 74-75 -1.8927066506054189 1101 76 -1.9369213144328157 1102 1 0.13738493933907847 1102 2 -0.6358077566714329 1102 3 -0.1069370436993502 1102 4 0.06571048542996749 1102 5 0.14995671928853938 1102 6 0.4424654272721753 1102 7 0.8726095981732911 1102 8 1.2391306080795204 1102 9 0.29706264789608383 1102 10-11 -0.9740908597844893 1102 12-13 -3.8408522149751363 1102 14-15 -1.3589687070610168 1102 16-17 -2.5555060978350497 1102 18-19 -1.0559546951702004 1102 20-21 -0.2255796282810394 1102 22-23 -0.843128987524068 1102 24-25 -0.16548250245898544 1102 26-27 -6.607896166925155 1102 28-29 -0.5684087863996794 1102 30-31 0.09101581767987454 1102 32-33 -6.692677269314366 1102 34-35 -0.9469200212428106 1102 36-37 0.04827345601276534 1102 38-39 0.2611684726478174 1102 40-41 0.09041600769666758 1102 42-43 0.3401643584865184 1102 44-45 -6.324634664200197 1102 46-47 -0.37485441800137664 1102 48-49 0.38924772835987653 1102 50-51 -6.072051188556145 1102 52-53 -2.0488611699882924 1102 54-55 0.45396607500953934 1102 56-57 -1.4452793032671494 1102 58-59 0.6356463031109385 1102 60-61 -0.08294926605540809 1102 62-63 -4.798130245965286 1102 64-65 -1.0188954917581015 1102 66-67 -0.1942302934907829 1102 68-69 -7.137716990410198 1102 70-71 -1.0861884968501556 1102 72-73 -0.02499200537287294 1102 74-75 -6.484784147421863 1102 76 -2.0895318292590837 1103 1 -0.03248657020126089 1103 2 -0.4233533811706778 1103 3 -0.4820965439812568 1103 4 -0.8497617837768345 1103 5 0.1291697523162263 1103 6 -1.2559974351273056 1103 7 0.2869839119511468 1103 8 1.0320176373682202 1103 9 0.056981339845400214 1103 10-11 -0.2753015782034325 1103 12-13 -1.0935080801343418 1103 14-15 -0.9153798765319152 1103 16-17 -0.6211979581276381 1103 18-19 -0.6374259554449893 1103 20-21 -0.4532644260495218 1103 22-23 -0.5578089483537667 1103 24-25 -0.07572464614278118 1103 26-27 -1.3364563615896827 1103 28-29 -0.22593571598216045 1103 30-31 -0.470343569839045 1103 32-33 -4.537368506443062 1103 34-35 -1.6229860766153763 1103 36-37 -0.3502398529874995 1103 38-39 0.06107351434044972 1103 40-41 -0.4108970876474132 1103 42-43 0.017187415550530716 1103 44-45 -4.980418218608342 1103 46-47 -0.6244612312599287 1103 48-49 -0.7098946794267036 1103 50-51 -0.11304647031524695 1103 52-53 -0.5782463149480819 1103 54-55 -0.2864713266937784 1103 56-57 0.40174137983918 1103 58-59 -0.05438900950961312 1103 60-61 0.15203545159199905 1103 62-63 -0.2242556502799573 1103 64-65 -0.2056740311806351 1103 66-67 -0.21311067569796194 1103 68-69 -3.3076552675104054 1103 70-71 -0.15259254444800519 1103 72-73 0.3547151075978334 1103 74-75 -2.3473319975064335 1103 76 0.512785746930124 1104 1 -2.4198502550941186 1104 2 -0.048953930244113764 1104 3 0.4448843147085917 1104 4 0.4863550468492619 1104 5 0.07056378876859881 1104 6 0.4498288441375493 1104 7 1.2915595239392488 1104 8 1.7542398595904416 1104 9 0.31088967508524235 1104 10-11 -0.14489663654248375 1104 12-13 -0.30354773543823654 1104 14-15 0.4524993273115925 1104 16-17 -0.19991754937296946 1104 18-19 1.0956252151490844 1104 20-21 1.3146567209038835 1104 22-23 0.5367918295002738 1104 24-25 0.7559118977449728 1104 26-27 0.9546478861034444 1104 28-29 0.28531266348859674 1104 30-31 -0.08391407239376036 1104 32-33 -0.1645133245624919 1104 34-35 0.49127076773756073 1104 36-37 0.04291960709028331 1104 38-39 -0.16515236837135916 1104 40-41 1.3290746960786635 1104 42-43 1.1107586807072423 1104 44-45 -2.7675569359117844 1104 46-47 0.6654419182863194 1104 48-49 1.3760505657498427 1104 50-51 1.5725327259022492 1104 52-53 0.7691379601987194 1104 54-55 0.39193941074235994 1104 56-57 1.0702573563510356 1104 58-59 1.3719055073820243 1104 60-61 0.5948974968905674 1104 62-63 1.2797480102096372 1104 64-65 0.5244601867710159 1104 66-67 0.6768649210380886 1104 68-69 -2.3449274401634987 1104 70-71 0.2270116651150218 1104 72-73 1.366363853117548 1104 74-75 -0.4364420409747467 1104 76 0.1860489208796352 1105 1 -1.0268485274439776 1105 2 0.16756962510515194 1105 3 -0.6673355867895658 1105 4 -0.42637283536110004 1105 5 0.10590325912779619 1105 6 0.07817777789376379 1105 7 0.9465838915419411 1105 8 0.514032025857432 1105 9 0.9111320618210144 1105 10-11 0.44056402718459964 1105 12-13 -1.405743398262814 1105 14-15 -0.6719108219883196 1105 16-17 0.640371509702355 1105 18-19 0.30931877642908745 1105 20-21 -0.46525483372337817 1105 22-23 -0.0013647420167046675 1105 24-25 -0.046220589813366075 1105 26-27 -0.0710219006477999 1105 28-29 -0.9458959180332798 1105 30-31 -0.21644030946861648 1105 32-33 0.48614034604941025 1105 34-35 0.2876981419672049 1105 36-37 0.790966331001485 1105 38-39 0.2925266497044987 1105 40-41 0.44498841671505573 1105 42-43 0.29527558331418646 1105 44-45 1.277439325143888 1105 46-47 1.0938024556628676 1105 48-49 0.2486363680757222 1105 50-51 0.027968377381064613 1105 52-53 -1.333425150602963 1105 54-55 -0.5531303399087335 1105 56-57 -0.4692852031497523 1105 58-59 -0.017524883788908596 1105 60-61 0.2386470134063785 1105 62-63 0.5970490081209832 1105 64-65 0.011747776054427561 1105 66-67 0.17923077029417556 1105 68-69 0.9668102664454068 1105 70-71 0.9986524170296178 1105 72-73 0.31334820112301287 1105 74-75 -1.3730475952410117 1105 76 -1.342895665386834 1106 1 -0.32393684104506804 1106 2 -0.08108499450624507 1106 3 -0.41310117904017574 1106 4 -0.5941913364657267 1106 5 0.5099705564710213 1106 6 -0.006765291272948559 1106 7 1.1081711571625164 1106 8 2.0477656688642867 1106 9 1.8135849026979187 1106 10-11 0.9927081386670622 1106 12-13 0.7466103093222927 1106 14-15 0.29245784056195134 1106 16-17 0.778464185168275 1106 18-19 0.1907836832437937 1106 20-21 0.0473917419294807 1106 22-23 0.660066742716765 1106 24-25 0.8530741476250228 1106 26-27 0.08297458720129214 1106 28-29 -0.7064182088620328 1106 30-31 -0.45421859078057736 1106 32-33 0.8691205760386538 1106 34-35 0.8738308106354182 1106 36-37 0.4717571314319997 1106 38-39 0.401261708901238 1106 40-41 -0.03273491642944748 1106 42-43 0.6634849528264581 1106 44-45 1.19789193943042 1106 46-47 0.6796377244557092 1106 48-49 0.608221141202101 1106 50-51 0.8137838195155425 1106 52-53 0.34861189692436767 1106 54-55 0.6311726114309764 1106 56-57 0.8445849605617965 1106 58-59 -0.10660322741322403 1106 60-61 1.191587023602949 1106 62-63 1.3220390947918048 1106 64-65 1.6018174525966629 1106 66-67 1.4463241821788415 1106 68-69 1.8955334296471307 1106 70-71 0.9766306643530172 1106 72-73 0.9373922729521666 1106 74-75 -1.6073420494414314 1106 76 0.3665021706750231 1107 1 -0.020620294482643686 1107 2 0.5313492727651159 1107 3 0.5536777085812048 1107 4 0.7370737924956217 1107 5 0.43347018085543354 1107 6 0.4402033517809443 1107 7 0.7919584752671867 1107 8 1.4613462592763469 1107 9 -0.7543736961168079 1107 10-11 0.43626146216947603 1107 12-13 0.6209808054131116 1107 14-15 -0.1116558457572907 1107 16-17 0.8163318015395546 1107 18-19 1.4980024584587284 1107 20-21 0.8085141369657975 1107 22-23 -0.21080496369095414 1107 24-25 -0.1664509073261442 1107 26-27 1.0290111471189363 1107 28-29 -0.3591963730756049 1107 30-31 0.47569432204878126 1107 32-33 1.217017128722219 1107 34-35 1.0773935940860753 1107 36-37 0.2838102184357041 1107 38-39 1.154850924560229 1107 40-41 0.9725515252388419 1107 42-43 0.8079926181668711 1107 44-45 0.49556729626522156 1107 46-47 0.5653114675346416 1107 48-49 0.9226392152679281 1107 50-51 1.2193721448264725 1107 52-53 1.2322127984017897 1107 54-55 1.0259237313219103 1107 56-57 1.017152501809953 1107 58-59 1.6317874684494384 1107 60-61 0.16610081008634836 1107 62-63 -0.320388773102799 1107 64-65 0.6468495885973269 1107 66-67 1.144600523961202 1107 68-69 2.002590829274297 1107 70-71 0.9193631982279769 1107 72-73 0.6700861323328198 1107 74-75 0.058276603350641665 1107 76 -0.03944090269521894 1108 1 -0.6155070124207036 1108 2 0.5994094473649945 1108 3 0.2559875194313399 1108 4 -0.028970169258688117 1108 5 0.6101616171472664 1108 6 0.9232370246140391 1108 7 1.7756676833320384 1108 8 1.5651058726623361 1108 9 0.1310061625404373 1108 10-11 0.4058623767402523 1108 12-13 0.950596529794737 1108 14-15 1.3675649503349945 1108 16-17 0.02325343945266667 1108 18-19 0.8450651793067863 1108 20-21 1.541833613166169 1108 22-23 0.46049518180893756 1108 24-25 -0.30824185515404423 1108 26-27 0.028348602949321844 1108 28-29 0.9197542089239761 1108 30-31 1.3024974814751005 1108 32-33 1.1486191865064583 1108 34-35 0.6616666142482046 1108 36-37 0.7862980068372849 1108 38-39 1.1000806065390307 1108 40-41 0.8127809473963907 1108 42-43 1.0227803442113483 1108 44-45 0.6646589611997484 1108 46-47 0.9525360570170847 1108 48-49 0.47182987209603056 1108 50-51 0.911789261849961 1108 52-53 0.8000651160072749 1108 54-55 1.3088300917459392 1108 56-57 1.5575741916873227 1108 58-59 -0.11828398239237714 1108 60-61 1.4726054349044801 1108 62-63 0.6252176496783335 1108 64-65 0.7643927189236592 1108 66-67 0.9015117690246015 1108 68-69 2.172137181384045 1108 70-71 2.2972796916804867 1108 72-73 1.0679504017154784 1108 74-75 1.7174755744585255 1108 76 1.2179380206055477 1109 1 -0.4252202215477716 1109 2 -0.16421050239426904 1109 3 0.6277820557512079 1109 4 0.15353192410534788 1109 5 0.6945896054244223 1109 6 0.7937362919366251 1109 7 -0.7909797941642935 1109 8 0.40746247722587015 1109 9 0.8598762693020063 1109 10-11 1.1130530822039262 1109 12-13 0.17446218815772596 1109 14-15 0.3908339999477235 1109 16-17 -0.14547170184940228 1109 18-19 0.6941532575986002 1109 20-21 1.062453248920825 1109 22-23 0.9076639069502477 1109 24-25 0.8323733322020459 1109 26-27 0.7225212008140858 1109 28-29 0.027490497389536017 1109 30-31 0.33489228993228437 1109 32-33 0.8470046794936863 1109 34-35 0.13606215538280964 1109 36-37 0.24073463024109287 1109 38-39 0.6303297168593147 1109 40-41 0.9342418538230248 1109 42-43 0.7421060193212554 1109 44-45 0.3551550884762804 1109 46-47 -0.7960116716829404 1109 48-49 -0.31134340611205147 1109 50-51 1.107329088100741 1109 52-53 0.676700556660407 1109 54-55 0.38823400633668115 1109 56-57 0.6396013530856806 1109 58-59 -0.0034564232548390805 1109 60-61 0.7126030798699361 1109 62-63 0.08430546629963942 1109 64-65 0.5193644585091732 1109 66-67 0.4373082940403066 1109 68-69 1.1770435033434339 1109 70-71 -0.2357365842248953 1109 72-73 0.0353200898042374 1109 74-75 1.6818949276090365 1109 76 -0.0662884813831468 1110 1 0.7432939792403275 1110 2 0.41267009645221364 1110 3 0.4619403028435549 1110 4 0.738116263015371 1110 5 0.307493710904307 1110 6 0.0561815817472322 1110 7 1.0836742192797502 1110 8 2.2216728097820138 1110 9 -1.4338884734748873 1110 10-11 -0.6347619189550429 1110 12-13 0.1853905910974163 1110 14-15 0.16495761429713696 1110 16-17 0.9372719204082713 1110 18-19 1.3229147961650227 1110 20-21 0.44041373487002033 1110 22-23 0.5696060675425443 1110 24-25 0.5629196841743394 1110 26-27 -0.38999430892807396 1110 28-29 0.5057427710154769 1110 30-31 0.32542964284539266 1110 32-33 1.4537662453299873 1110 34-35 0.6840775901328087 1110 36-37 0.961409286981521 1110 38-39 0.6843717942784977 1110 40-41 0.9364779836821739 1110 42-43 1.1541402157461746 1110 44-45 1.8671606515310515 1110 46-47 0.6616722977202585 1110 48-49 1.2531362164728606 1110 50-51 0.3426476684309847 1110 52-53 0.3104183952500108 1110 54-55 -0.10663925691306275 1110 56-57 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0.3597705290196487 2116 40-41 0.3569983436572244 2116 42-43 0.9022690583016342 2116 44-45 2.2370483820843816 2116 46-47 0.5773365586130694 2116 48-49 0.6673525579145476 2116 50-51 0.7424293667291195 2116 52-53 1.3068765615157147 2116 54-55 0.8577642002730741 2116 56-57 0.3130888975696706 2116 58-59 -0.3586938091137668 2116 60-61 -0.9081657689258975 2116 62-63 1.2968147769013143 2116 64-65 1.4034716062925625 2116 66-67 0.8486161921892119 2116 68-69 0.8834629907775664 2116 70-71 -2.0914429485383934 2116 72-73 1.1686685959699261 2116 74-75 -0.2151367146431653 2116 76 -0.4417747016926725 2117 1 -0.5121683796703067 2117 2 0.020045945207371574 2117 3 0.38332967250878625 2117 4 -0.4046373280140827 2117 5 -1.5877858050648648 2117 6 -2.0149585238756273 2117 7 -2.828380692775159 2117 8 -3.7150866097360264 2117 9 -0.5835208644865872 2117 10-11 -0.8836561333664932 2117 12-13 -0.03408011113189957 2117 14-15 -1.0753787812116826 2117 16-17 -0.8311640032000653 2117 18-19 -0.7899492994576889 2117 20-21 -0.5591202819053791 2117 22-23 -0.4367623698603751 2117 24-25 -0.2551175648973967 2117 26-27 0.39586790796862914 2117 28-29 0.16361492888763962 2117 30-31 -0.5620834860470438 2117 32-33 0.6159284074921487 2117 34-35 -1.4174697700352397 2117 36-37 -2.1225638487157497 2117 38-39 -0.3999193934609693 2117 40-41 -2.742429988329249 2117 42-43 -2.103859162942861 2117 44-45 -0.3962086290796023 2117 46-47 -1.241181294788504 2117 48-49 -1.328285106327769 2117 50-51 0.08497373834325828 2117 52-53 0.8260843074676458 2117 54-55 -1.7338822634664162 2117 56-57 -1.8403860059052306 2117 58-59 -1.2930866737391895 2117 60-61 0.5900608630216695 2117 62-63 -1.0264545056703014 2117 64-65 -1.381644074171959 2117 66-67 -2.098601789912486 2117 68-69 -1.067855437285047 2117 70-71 -0.1346772127028899 2117 72-73 0.12208962497235376 2117 74-75 -1.056236951092238 2117 76 -1.1804835101689228 2118 1 0.6396229465396246 2118 2 0.5714511710312031 2118 3 0.014109954451861029 2118 4 -0.003975869213089567 2118 5 0.01750885836933236 2118 6 0.7765771978926495 2118 7 0.8885837800032661 2118 8 -4.208292440151158 2118 9 -0.7502922870719644 2118 10-11 -0.49404642398934584 2118 12-13 0.40142017320559376 2118 14-15 -0.6165179270019863 2118 16-17 0.060189143967036784 2118 18-19 -0.636766216042048 2118 20-21 -2.232334193491379 2118 22-23 -0.3382394283141821 2118 24-25 -0.1562953207263149 2118 26-27 0.44549976574001704 2118 28-29 -0.20808151839020184 2118 30-31 -0.109837219847293 2118 32-33 -0.0035980957552794735 2118 34-35 1.1581041427479732 2118 36-37 -0.7338370820820046 2118 38-39 0.10201859103515432 2118 40-41 -0.7961024315365748 2118 42-43 -0.5647852052642506 2118 44-45 0.25061427355725385 2118 46-47 -0.2501828212318884 2118 48-49 -1.1214071320079313 2118 50-51 -0.4804388503251431 2118 52-53 -1.1698676245308022 2118 54-55 -0.0027009160059989767 2118 56-57 1.2181276572595934 2118 58-59 0.926189911816472 2118 60-61 -1.7434370867553497 2118 62-63 0.22898531953696732 2118 64-65 -0.13722606812604 2118 66-67 -2.1591357457952824 2118 68-69 1.0753234558938445 2118 70-71 -0.5662491500887796 2118 72-73 -1.2615639621696069 2118 74-75 0.20346793651962258 2118 76 -0.30223981797174204 2119 1 -0.007663875165800249 2119 2 0.3551988320843975 2119 3 -0.07828958221391602 2119 4 0.11359519021843667 2119 5 0.004811718567140133 2119 6 0.3626949917089206 2119 7 -2.3071303784213946 2119 8 0.4324007791306741 2119 9 -0.8706700826702942 2119 10-11 0.005084824339981253 2119 12-13 0.1279193267295966 2119 14-15 -0.8561151826377369 2119 16-17 -0.7428047079442166 2119 18-19 -1.4644415256740544 2119 20-21 -1.2128429701108274 2119 22-23 0.32822675719771865 2119 24-25 -0.03095004762642617 2119 26-27 0.4720746565891716 2119 28-29 0.6724094376821483 2119 30-31 -0.3201642268864049 2119 32-33 -0.1515977393443393 2119 34-35 0.39288985066920645 2119 36-37 -0.3700083375395451 2119 38-39 -0.1719500448019673 2119 40-41 -0.19379021555154452 2119 42-43 -0.683024535211679 2119 44-45 -0.590693754599215 2119 46-47 0.08695965404209716 2119 48-49 -0.6434155076650683 2119 50-51 1.0706170170899867 2119 52-53 -1.1857501871254712 2119 54-55 -1.125796345035674 2119 56-57 0.5784145680677568 2119 58-59 1.0435159387254913 2119 60-61 -0.2084642630896667 2119 62-63 -0.29199837466244105 2119 64-65 0.050847530733449275 2119 66-67 -0.12260362425914906 2119 68-69 -1.1650980000448499 2119 70-71 -1.1588491679093238 2119 72-73 -2.2286182257355023 2119 74-75 -0.15396768675400807 2119 76 0.8418393052573414 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 13.0 13 97.0 14 336.0 15 711.0 16 903.0 17 991.0 18 1013.0 19 937.0 20 876.0 21 739.0 22 738.0 23 821.0 24 927.0 25 1063.0 26 1311.0 27 1491.0 28 1874.0 29 2351.0 30 2821.0 31 3608.0 32 4644.0 33 6066.0 34 8410.0 35 12239.0 36 20508.0 37 23875.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.77467358689482 21.51589489903495 11.100072986781283 31.609358527288943 2 18.28794557494515 21.22657649497816 34.44840287422257 26.037075055854114 3 17.48284121329227 23.934746291488036 27.630175311474748 30.95223718374494 4 13.243966749189864 17.42245838616831 37.5349717207093 31.79860314393253 5 13.047722561036975 35.31489644345148 35.56347241511181 16.073908580399735 6 28.12028259163094 36.033452086226674 18.668357385826138 17.177907936316245 7 27.933095827546644 29.17295654449208 22.036712758891372 20.857234869069906 8 23.510053740716142 32.53426725439285 20.70627780125999 23.24940120363102 9 23.204114083288047 16.040698025481554 22.007527725781454 38.74766016544894 10-11 24.12696495783266 25.04981583237727 27.518970271521447 23.304248938268625 12-13 23.619749209991344 23.795362598876878 26.45723889459171 26.127649296540064 14-15 21.488738602741382 25.26417486866735 24.184831833826458 29.06225469476481 16-17 21.840971760964514 27.630678501700785 26.091419600265688 24.43693013706902 18-19 21.48018436889882 26.987098202604514 26.424028339673526 25.108689088823137 20-21 20.847674254775274 27.62514340921442 26.373709317070226 25.15347301894008 22-23 22.38139806372401 26.983575871022282 26.497494112674357 24.13753195257935 24-25 21.83392709780005 27.734335688263588 26.122617394279736 24.309119819656622 26-27 20.43757422055834 28.088078417164823 27.213030614093352 24.261316748183486 28-29 21.488738602741382 26.789344443773523 28.591771833423905 23.130145120061187 30-31 21.56321075619427 27.06056397560534 27.57633395728921 23.79989131091118 32-33 20.167864259404624 28.392508503914822 27.13704888996236 24.302578346718192 34-35 21.57780327274923 27.46060020530161 27.321719702916493 23.63987681903267 36-37 21.886258881307487 26.950868506330135 27.009741762776002 24.153130849586375 38-39 21.67491898637361 27.456574683493347 27.667914578427226 23.200591751705815 40-41 21.50383430952237 26.384779502042953 28.00454883964334 24.106837348791338 42-43 20.996618561681057 26.30376587565163 28.049835959986314 24.649779602680997 44-45 20.467262443894292 26.132178008574364 28.57214741460862 24.828412132922733 46-47 20.696717186965362 26.151802427389647 28.65316104099994 24.49831934464505 48-49 19.93438399452529 26.3626391320975 29.271078638568525 24.43189823480869 50-51 19.570074270877363 26.18652255298593 29.216230903930924 25.02717227220578 52-53 19.190668840448446 26.75110198659501 29.00841334057927 25.04981583237727 54-55 18.842964394259607 26.87438359197311 28.718575770384234 25.56407624338305 56-57 19.27923032023026 26.473340981824766 28.855946702091256 25.391481995853717 58-59 19.20475816677737 26.43761447577642 28.316023589557798 26.04160376788841 60-61 19.33709719622406 26.39081778475535 28.215385544351186 26.056699474669404 62-63 19.43069057826621 25.956564619688827 28.438802004709864 26.173942797335105 64-65 19.916772336614134 26.23583519513717 27.44047259626029 26.406919871988404 66-67 20.13767284584264 26.301249924521464 26.8301028520822 26.73097437755369 68-69 19.49207978584224 26.131171628122296 27.132520177928065 27.2442284081074 70-71 20.3319042730914 26.783809351287164 26.559889700702456 26.324396674918987 72-73 20.46374011231206 26.43006662238593 26.461767606626008 26.644425658676006 74-75 20.19302377070628 27.21655294567558 26.397862447919813 26.192560835698327 76 20.319827707666608 26.376225268200393 26.75764345953344 26.54630356459956 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.0 9 0.5 10 1.5 11 2.0 12 1.0 13 0.5 14 1.0 15 3.0 16 6.5 17 9.0 18 11.5 19 22.5 20 29.5 21 28.0 22 38.5 23 64.5 24 98.0 25 116.0 26 169.5 27 256.0 28 390.5 29 492.0 30 583.5 31 800.5 32 1134.5 33 1343.0 34 1574.0 35 2096.5 36 2642.0 37 2896.0 38 3242.0 39 4130.5 40 5114.0 41 5883.0 42 6211.0 43 6432.0 44 6959.5 45 7329.0 46 7392.0 47 7477.0 48 7237.0 49 6576.5 50 6241.0 51 5821.5 52 4935.0 53 4025.0 54 3582.0 55 3414.0 56 2812.5 57 2144.5 58 1910.0 59 1626.5 60 1186.0 61 894.0 62 759.0 63 648.0 64 452.5 65 340.5 66 261.0 67 209.0 68 164.5 69 96.0 70 59.0 71 46.0 72 36.0 73 26.5 74 22.0 75 17.0 76 13.5 77 12.5 78 12.5 79 10.0 80 9.0 81 4.5 82 2.0 83 3.0 84 3.0 85 2.5 86 2.5 87 3.0 88 4.0 89 3.0 90 1.0 91 0.5 92 0.0 93 0.0 94 2.0 95 3.5 96 3.0 97 3.5 98 3.0 99 5.5 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7225811645834591 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 99366.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.40257230843548 #Duplication Level Percentage of deduplicated Percentage of total 1 74.4949494949495 39.782219270172895 2 9.699608020503543 10.359680373568423 3 5.314337403889643 8.513978624479199 4 3.1151062867480777 6.654187549061047 5 1.9768581335745514 5.278465471086689 6 1.3474295190713101 4.317372139363565 7 0.8367254635911352 3.127830445021436 8 0.642620232172471 2.7454058732363182 9 0.46547565204281616 2.237183744942938 >10 2.1031207598371777 16.83875772396997 >50 0.003769033619779888 0.14491878509751827 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.018114848137189783 0.0 2 0.0 0.0 0.0 0.01912122858925588 0.0 3 0.0 0.0 0.0 0.02012760904132198 0.0 4 0.0 0.0 0.0 0.026165891753718577 0.0 5 0.0 0.0 0.0 0.027172272205784675 0.0 6 0.0 0.0 0.0 0.027172272205784675 0.0 7 0.0 0.0 0.0 0.027172272205784675 0.0 8 0.0 0.0 0.0 0.027172272205784675 0.0 9 0.0 0.0 0.0 0.03220417446611517 0.0 10 0.0 0.0 0.0 0.03924883763057786 0.0 11 0.0 0.0 0.0 0.04327435943884226 0.0 12 0.0 0.0 0.0 0.04729988124710666 0.0 13 0.0 0.0 0.0 0.04830626169917276 0.0 14 0.0 0.0 0.0 0.06138920757603204 0.0 15 0.0 0.0 0.0 0.06541472938429643 0.0 16 0.0 0.0 0.0 0.06843387074049474 0.0 17 0.0 0.0 0.0 0.07044663164462693 0.0 18 0.0 0.0 0.0 0.07648491435702352 0.0 19 0.0 0.0 0.0 0.07749129480908963 0.0 20 0.0 0.0 0.0 0.08252319706942013 0.0 21 0.0 0.0 0.0 0.09258700159008111 0.0 22 0.0 0.0 0.0 0.1076827083710726 0.0 23 0.0 0.0 0.0 0.1187528933437997 0.0 24 0.0 0.0 0.0 0.1187528933437997 0.0 25 0.0 0.0 0.0 0.12781031741239457 0.0 26 0.0 0.0 0.0 0.13586136102892338 0.0 27 0.0 0.0 0.0 0.18114848137189785 0.0 28 0.0 0.0 0.0 0.213352655838013 0.0 29 0.0 0.0 0.0 0.23649940623553328 0.0 30 0.0 0.0 0.0 0.27474186341404505 0.0 31 0.0 0.0 0.0 0.30392689652396193 0.0 32 0.0 0.0 0.0 0.34418211460660586 0.0 33 0.0 0.0 0.0 0.38745647404544814 0.0 34 0.0 0.0 0.0 0.4488456816214802 0.0 35 0.0 0.0 0.0 0.5504901072801562 0.0 36 0.0 0.0 0.0 0.7225811645834591 0.0 37 0.0 0.0 0.0 1.026508061107421 0.0 38 0.0 0.0 0.0 1.432079383290059 0.0 39 0.0 0.0 0.0 1.899039913048729 0.0 40 0.0 0.0 0.0 2.467644868466075 0.0 41 0.0 0.0 0.0 3.2184046857073847 0.0 42 0.0 0.0 0.0 4.03759837368919 0.0 43 0.0 0.0 0.0 4.938308878288348 0.0 44 0.0 0.0 0.0 5.783668458023872 0.0 45 0.0 0.0 0.0 6.5595877865668335 0.0 46 0.0 0.0 0.0 7.403940985850291 0.0 47 0.0 0.0 0.0 8.276472837791598 0.0 48 0.0 0.0 0.0 9.016162470060182 0.0 49 0.0 0.0 0.0 9.684399090232072 0.0 50 0.0 0.0 0.0 10.426101483404786 0.0 51 0.0 0.0 0.0 11.197995290139485 0.0 52 0.0 0.0 0.0 11.945735966024596 0.0 53 0.0 0.0 0.0 12.765936034458466 0.0 54 0.0 0.0 0.0 13.543868123905561 0.0 55 0.0 0.0 0.0 14.287583277982408 0.0 56 0.0 0.0 0.0 14.987017692168347 0.0 57 0.0 0.0 0.0 15.67739468228569 0.0 58 0.0 0.0 0.0 16.385886520540225 0.0 59 0.0 0.0 0.0 17.021918966245998 0.0 60 0.0 0.0 0.0 17.662983314212106 0.0 61 0.0 0.0 0.0 18.30404766217821 0.0 62 0.0 0.0 0.0 18.979328945514563 0.0 63 0.0 0.0 0.0 19.590201879918684 0.0 64 0.0 0.0 0.0 20.312783044502144 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAAATGC 15 0.0022199228 69.99195 22 CTGTCAG 15 0.0022199228 69.99195 14 GCGACTG 15 0.0022199228 69.99195 67 AGAACTT 15 0.0022199228 69.99195 12 ATCGTCA 15 0.0022199228 69.99195 31 GCACATC 15 0.0022199228 69.99195 15 CAGATGA 15 0.0022199228 69.99195 67 TCGTCAC 15 0.0022199228 69.99195 32 CCAAGGG 15 0.0022199228 69.99195 31 CATCGTT 15 0.0022199228 69.99195 32 TACTAGC 15 0.0022199228 69.99195 70 TATCGTC 15 0.0022199228 69.99195 30 CGTCACA 15 0.0022199228 69.99195 33 GTTACTA 25 2.3817364E-4 55.99356 68 AGGTAAC 20 0.006936606 52.49396 6 TCGTTGT 20 0.006936606 52.49396 34 ATTAGAG 20 0.006936606 52.49396 3 CGTATAC 20 0.006936606 52.49396 24 TTTAACG 20 0.006936606 52.49396 34 TTAGAGA 20 0.006936606 52.49396 46 >>END_MODULE