FastQCFastQC Report
Wed 25 May 2016
SRR1780644_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780644_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99366
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT10111.017450637038826TruSeq Adapter, Index 5 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT5010.5041966064851157TruSeq Adapter, Index 5 (96% over 26bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1570.15800173097437756No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC1280.12881669786446068RNA PCR Primer, Index 5 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTTGT207.8930745E-570.0000123
CGCGAGA150.002218910570.022
TTGACAA150.002218910570.055
GCTCGCG150.002218910570.019
GCTCGCC150.002218910570.034
GGCTCGC150.002218910570.018
TTGGCGT308.324114E-658.33333222
ATTGGCG308.324114E-658.33333221
GCGTGAA308.324114E-658.33333225
CGCCATT308.324114E-658.33333217
GTCTCGA252.3803841E-455.99999660
TAGCCCG252.3803841E-455.9999965
ACGTCTC252.3803841E-455.99999658
AACGTTA200.006933450752.5000048
TCTGATG200.006933450752.50000441
CGGAAAA200.006933450752.50000415
GGTACTA200.006933450752.50000460
ACGTTAT200.006933450752.5000049
GTTTACC200.006933450752.50000467
CCGGAAA200.006933450752.50000414
Produced by FastQC (version 0.11.5)