FastQCFastQC Report
Wed 25 May 2016
SRR1780643_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780643_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTTCTGCTTG21.8691588785046727Illumina PCR Primer Index 3 (96% over 28bp)
GCGCAGGTAGGCGACGGCGTGGGCCTGCACCTCGCCTGTCTCTTATACAC21.8691588785046727No Hit
GCGCAAGGCCGCGATCCTCGGCGCCATGGCCGAGTACCTCCTGTCTCTTA10.9345794392523363No Hit
CTTCCTGTCCGCGCTGGCCGGCGTCACCGACCCCGAGACCTGTCTCTTAT10.9345794392523363No Hit
GTCACCAGGCTGAGTAGGGATGCAAGCCCAGATACACCTGACCCCCGGCC10.9345794392523363No Hit
ATACTACAAGGACAGGCCCATTTGAGTATTTTGTTTTCAATTAGGGAGAT10.9345794392523363No Hit
GCTGACGCCGGGCGTGCTCTGGCTGCCGGCACCGATCCCGACGCCGACGG10.9345794392523363No Hit
GCCCGCACGTGATCCGGGCAACACGGAGGGCGGCGGGGGGGTGGCCTCGG10.9345794392523363No Hit
CACGTCCTCGGCCGCGCCCGCCGTGCGGGGGTCCATGTCCAGGCCGAGCT10.9345794392523363No Hit
GGGTATGGGGTTAGCAGCGGTGTGTGTGTGCTGGGTAGGATCTGTCTCTT10.9345794392523363No Hit
GCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCAC10.9345794392523363No Hit
CTCTACGTGAACGTGGACGGGCGATCGGGCTACTTCGCCCGCTCCTTGGC10.9345794392523363No Hit
GGACCACTGCATCCACGCCGTCGAGCCCCTGTCTCTTATACACATCTCCG10.9345794392523363No Hit
GGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACT10.9345794392523363No Hit
GTGCTCGGCTTCCGGTGTGAAGTCTCGCCACCCGGGCCCTGTCTCTTATA10.9345794392523363No Hit
AGCGTTGAGCCCGCTGGGGTTCGGCGCCACGAACAGCCGCGTGCCCGGCC10.9345794392523363No Hit
GCCCAGCGCGTGGTCGATCGGCACCGAGAACCAGTGGTCCACCGTGACGG10.9345794392523363No Hit
ACCAGCACGCGCCGCCGGTCGCCCTCGTCCCGGTGGCGGCGGACTGTCTC10.9345794392523363No Hit
CTACTCCAAAGACCACATCATCGAAACCGCAAACATATCATACACAAACG10.9345794392523363No Hit
CATAAATACTTGACCACCTGTAGTACATAAAAACCCAATCCACATCAAAA10.9345794392523363No Hit
CACCACCGTCGTGCGCGTCAGCGCGCGTCCCCGCTGCTGTCTCTTATACA10.9345794392523363No Hit
TCACGATCCTCGCCAAGAACACCACGGTGTTCTACTCCGGCCCTGTCTCT10.9345794392523363No Hit
GGCTCCGGCCGCAGACCGGGCTGGCGGTGACGAGCTGGTGCATGCGGCTG10.9345794392523363No Hit
CTCCCCGGCTGGATGAGAAGGGTCGGCTGATCCTGCCCGCTGTCTCTTAT10.9345794392523363No Hit
GCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGC10.9345794392523363No Hit
CCCTAGAAGTCCCACTCCTATACACATCTCCGAGCCCACGAGACTTATGA10.9345794392523363No Hit
CATGTGGTCAGGCCTCAGGACCCTGGGCCCCACCGGGAGATGTGCTCCTG10.9345794392523363No Hit
CCTGAAACATCGGCATTATCCTCCTGCTTGCAACTATAGCAACTGTCTCT10.9345794392523363No Hit
CGCTCGAGGAGATCGACCTCGCCGAGCCGCAGGAGGGCGAGGACTGTCTC10.9345794392523363No Hit
CAAATAGCCCTTCTAAACGCTAATCCAAGCCTCACCCCACTACTAGGCCT10.9345794392523363No Hit
GTCTGGGAAGGCACGATCCATCTCAGGCCTCTCTGAGCTCCTGCCCCAGG10.9345794392523363No Hit
GCGGCGTCGAAGTCCGCCGGGCCCCGGACCTCGTCCCCGCGGCGGCTGCC10.9345794392523363No Hit
CAGATCTCCACTCGCGTCCCCGCGCCGATCACGGTCACGTCCCTGTCTCT10.9345794392523363No Hit
GGCCGATGGTCGCCCCGGACCCCCAGGGCCGGCAGACGGCCGTCTCCCCT10.9345794392523363No Hit
GTTCTACTCGTCGCTGCAGTCCGCGACGGCGGCGCTCGAGAAGGCTGTCT10.9345794392523363No Hit
GCCGCGATCTGCGAGCCCATGGTGCCAGCTCCGAGGACGACGACGCCTGT10.9345794392523363No Hit
CTTATACACATCTCAGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT10.9345794392523363No Hit
CCTCTCTATACTAATCTCCCTACAAATCTCCTTAATTATAACATTCACAG10.9345794392523363No Hit
CTTATACACATCTCCGAGCCCACGAACCGGTTCCCATCTCGTATGCCGTC10.9345794392523363No Hit
ATAAAGCTCCTCCTGGAGACACTTTATCTCCAGATACTTTCTGGAGTCCT10.9345794392523363No Hit
TCTCCACTCCGAGCCCACGAGACTAACAACAATAGCGTATGCCGTCTTAT10.9345794392523363No Hit
CTTATACACATCTCCAAGCCCACGAGACTTTAATGCATCTCGTATGCCGT10.9345794392523363No Hit
AGCGGGGCCCGCGCGTCGTCATCCGGGGACGCCCAGACCGGGGCGCGGCA10.9345794392523363No Hit
CTTCAGTTCTGGGGCGAAGTTCTGCTCTTGATTACGACTTCAGGCTCTGG10.9345794392523363No Hit
GAGTAGGTAAAAACCAATTTCTTTCTTTGGTGGAGGTGAGGTAGGGAATG10.9345794392523363No Hit
GACCGGCGGCCTGAATGCCGAACAGCTTCTCGCCCTGGCCGCCTCCTGTC10.9345794392523363No Hit
CCCAGGGGCCGTGCACGCGGTAGCCGTAGCGCCTGTCTCTTATACACATC10.9345794392523363No Hit
CTCTCCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCTACTTCT10.9345794392523363No Hit
GCCGCACCGCGAGCGCCCGGGCGGCCTCGGGGGCGAGCCTGCCTCTTATA10.9345794392523363No Hit
TCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTA10.9345794392523363No Hit
GCCCCTCGCCGTCCCCGGCCCCGGCCGGGTGCACGTAGTAGGGCCTGTCT10.9345794392523363No Hit
ACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAAGCTATCGGGC10.9345794392523363No Hit
ACCCTAGCCAACCCCTTATACACATCTCCGAGCCCACGCGACTTATGAAA10.9345794392523363No Hit
GCTGAAGATGCCCCACGCCCGCGGTATCTGTCTCTTATACACATCTCCGA10.9345794392523363No Hit
GAGTAGAACATCGCCACCATCTCCATGGGCTGGAACACGCGCTGTCTCTT10.9345794392523363No Hit
CGCCTGACGGGTGGCGCGCCCGACGCGCTTTCGAACGTGGCAGAGGCTGT10.9345794392523363No Hit
GTCGATGCGCCTCGGCCTCGAGATCGGGGTAGTGGTTGTCCTGTCTCTTA10.9345794392523363No Hit
GGGCAGCCATCCCGCCGAGAACGACGACCCCGCCCGCCCCTGTATCTTAT10.9345794392523363No Hit
GCGAAGGTCAAGGACGCGCGGGTCACCCTCGTGCCGGCCCACGGGGTGCG10.9345794392523363No Hit
ATCCCCTATCTAGGCCTTCTCGCGAGCCAAAACCTGCCCCCTGTCTCTTA10.9345794392523363No Hit
TCCTTGAACAGCTCGCGCAGCGGCTCGCACAGCTCGAACTCGATGTCGTC10.9345794392523363No Hit
TAAGCACCCTAATCAACTGGCTTCAATCTACTTCTCCCGCCGCCGGGAAA10.9345794392523363No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCCTATACCTCGTATGCCGTC10.9345794392523363No Hit
CAGATAGGGGTCCCTTGACCACCATCCTCCGTGAAATCAATATCCCGCAC10.9345794392523363No Hit
TCCATACAATGTCTGGAATCTATAGATAACATAACCGGTTAGGTCAGAGG10.9345794392523363No Hit
CCTATAATCACTGCGCCCGCTCATAAGGGGATGGCCATGGCTAGGTTTAT10.9345794392523363No Hit
ATAATATTCATCCCTGTAGCATTGTTCGTTACATGGTCCATCATAGAATT10.9345794392523363No Hit
TGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCC10.9345794392523363No Hit
AGACGTAAATTATGGCTGAATCATCCGCTACCTTCACGCCAATGGTGCCT10.9345794392523363No Hit
GTTGGTGGGACTGTAAACTAGTTCAACCATTGTGGAAATCAGAGTGGTGA10.9345794392523363No Hit
CTTATACACATCTCCGAGGCCACGAGACCTCATGGGATCTCGTATGCCGT10.9345794392523363No Hit
CCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAGCCCCCATTCGTA10.9345794392523363No Hit
GCACAACACCGCCGAACAGCCCCTGGGGCTCACCGGCCCGGACCTGGGCC10.9345794392523363No Hit
CTCTTATACACATCTCCGAGCCCGCGAGACAAACGGTCATCTCGTATGCC10.9345794392523363No Hit
GCCCTGGTCCGTCCACGCGGAGAGGTCCCCGTGGGCGGGCGGGGTGATGC10.9345794392523363No Hit
ACACAGACACACCCTCACCACATACATACCACACACACACACCCTCACCA10.9345794392523363No Hit
GCACGGGGCCGTGAACCTCGGCCAGGGCTTCCCCGACGCCGACGGCCCCG10.9345794392523363No Hit
CCCGCACCCGATCTCCGGCGACGCCGCCCCCGTCACGCTCGGCCACGAGA10.9345794392523363No Hit
GAATGTGGTATTGTTTTGACTTACAGTAGTCACCCCTTATCCCTGGTTTC10.9345794392523363No Hit
GCAGAACCCAACCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG10.9345794392523363No Hit
GTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGCTGTCTCTTAT10.9345794392523363No Hit
GGGCCGGGGCGCCGCGCTGCGAGAGCTGCGAGTTCACAATCGAGGCGGAG10.9345794392523363No Hit
ACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCTTCT10.9345794392523363RNA PCR Primer, Index 19 (96% over 25bp)
GTCCTGTGGTGATTTGGAGGATCAGGCAGGCGCCAAGGAGCTGTCTCTTA10.9345794392523363No Hit
CACGGGGATGCCTGCCTTGGTCCTTCACCCTTAGTGGCAGATACCACTTT10.9345794392523363No Hit
CTTATACACATCTCCGAGCCTACGAGACCGGTTCCCATCTCGTATGCCGT10.9345794392523363No Hit
ACATAAGTGGAAATGTAAAACAGTACATCTGCTATGGAAAACCTGTCTCT10.9345794392523363No Hit
ATACACATCTCCGAGCCCACGAGATTTAATGCATATCGTATGCCGTCTTC10.9345794392523363No Hit
CTCCTGGGCCACTTTCAAGGGCGTGGCGGTCGTAGACCTGTCTCTTATAA10.9345794392523363No Hit
GCCGAGGGCGTCTTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAG10.9345794392523363No Hit
CTTATACACATCTCCGAGCCCACGAGACTTATGAAATCTCGTATGCCGTC10.9345794392523363No Hit
CCCACGAGACAAACGGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA10.9345794392523363Illumina PCR Primer Index 7 (96% over 28bp)
ACCCTACTAAACCCCATTAAACGCCTGGCAGCCGGAAGCCTATTCGCAGG10.9345794392523363No Hit
TCTTATACACATCTCCGACCCACGAGACTGGTCACAATCTCGTATGCCGT10.9345794392523363No Hit
TCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCTTCTGCTTG10.9345794392523363RNA PCR Primer, Index 36 (96% over 30bp)
CTTATACACATCTCCGAGCCCAGGAGACACCGGCTAATCTCGTATGCCGT10.9345794392523363No Hit
CTGCTGAACGTGGTGGCCGTACTCGTGCGCCACGATGTACTCCTCCTGTC10.9345794392523363No Hit
CCCCTACGGGCTACTACAACCCTTCGCTGACGCCATAAAACCTGTCTCTT10.9345794392523363No Hit
TCCATGCGCGCGGACCTGGGGATCCGCGAGCACGGCGCCGAGTCGCTGGA10.9345794392523363No Hit
CTTATACACATCTCCGACCCACGGACCGGTTCCCATCTCGTATGCCGTCT10.9345794392523363No Hit
TACGTGCACAACCGCGTCACCACCATCGACAAGGTGGCCAAGCTGTCTCT10.9345794392523363No Hit
ACCCTGGAGCCTCCGTAGACCTAACCATCTTCTCCTTACACCTAGCAGGT10.9345794392523363No Hit
TGTCACGCATGATGGACATGACCTGGATCTCGCTGCGGTCCCCGGGCACG10.9345794392523363No Hit
GACCACCCCCGCCCCCGACTGGCGCCCGCCCTGGCAGTCCCTGTCTCTTA10.9345794392523363No Hit
CTCCCAAAGTGCTGGGATTACAAGCATGAGCCATTACGCCAGGTCTTTAT10.9345794392523363No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)