FastQCFastQC Report
Wed 25 May 2016
SRR1780642_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780642_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52436
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCAGA150.002155728670.4639741
GAAGCTC150.002213878369.99332441
CTACTCG150.002213878369.99332465
CAAGTCG150.002213878369.99332460
TCGATTG150.002213878369.99332469
GAGACCT150.002213878369.99332428
CGGGAGT150.002213878369.99332457
ACTCGAT150.002213878369.99332467
TACTCGA150.002213878369.99332466
CTCGATT150.002213878369.99332468
AGTACTA150.002213878369.99332461
AGTCGTA150.002213878369.99332462
AAGTCGT150.002213878369.99332461
CTGTAGT200.006917862752.494992
GGAGACC200.006917862752.4949927
GAGTACT200.006917862752.4949960
GAGACAA200.006917862752.4949956
ATATAGA200.006917862752.4949948
GGAGTAC200.006917862752.4949959
TCGTAAC200.006917862752.4949964
Produced by FastQC (version 0.11.5)