FastQCFastQC Report
Wed 25 May 2016
SRR1780642_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780642_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52436
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT11522.1969639179189873No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT4750.9058661987947211RNA PCR Primer, Index 48 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC1220.23266458158517048No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG1030.19642993363338163No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC640.12205355099549928No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT630.1201464642611946No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCAAG150.00221304170.021
TAACACG150.00221304170.04
CATACCA150.00221304170.019
AACACGA150.00221304170.05
GTACACT200.006915251352.541
TGGTTCG200.006915251352.517
GTTACGA200.006915251352.554
TCCAGTA200.006915251352.537
TGATTCG200.006915251352.543
TCGTCCA200.006915251352.521
TGCCTTC200.006915251352.510
TTCGGGA200.006915251352.546
ACCAAGG200.006915251352.522
TGAATAA200.006915251352.512
GATTCGG200.006915251352.544
CGTCCAA200.006915251352.522
ACATACC200.006915251352.518
ATGTTAC200.006915251352.552
GGTTAAG200.006915251352.58
GCACTTT200.006915251352.531
Produced by FastQC (version 0.11.5)