##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780642_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 52436 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.843809596460446 34.0 34.0 34.0 31.0 34.0 2 33.33812647799222 34.0 34.0 34.0 33.0 34.0 3 33.50680829964147 34.0 34.0 34.0 34.0 34.0 4 33.48842398352277 34.0 34.0 34.0 34.0 34.0 5 33.50957357540621 34.0 34.0 34.0 34.0 34.0 6 37.239377526889925 38.0 37.0 38.0 36.0 38.0 7 36.84577389579678 38.0 37.0 38.0 35.0 38.0 8 36.90954687619193 38.0 37.0 38.0 35.0 38.0 9 36.70874971393699 38.0 37.0 38.0 34.0 38.0 10-11 36.44303531924632 38.0 37.0 38.0 34.0 38.0 12-13 36.3172057365169 38.0 37.0 38.0 34.0 38.0 14-15 36.5888034937829 38.0 37.0 38.0 34.0 38.0 16-17 36.714671218247005 38.0 37.0 38.0 34.5 38.0 18-19 36.72560836066825 38.0 37.0 38.0 34.0 38.0 20-21 36.46381302921657 38.0 37.0 38.0 34.0 38.0 22-23 36.54046838050195 38.0 37.0 38.0 34.0 38.0 24-25 35.90214737966283 38.0 36.5 38.0 32.5 38.0 26-27 36.12208215729652 38.0 37.0 38.0 33.5 38.0 28-29 35.99422152719506 38.0 37.0 38.0 33.0 38.0 30-31 35.72116484857732 38.0 36.5 38.0 31.5 38.0 32-33 36.210303989625444 38.0 37.0 38.0 34.0 38.0 34-35 36.23180639255473 38.0 37.0 38.0 33.5 38.0 36-37 36.347270958883215 38.0 37.0 38.0 34.0 38.0 38-39 35.95714776108018 38.0 36.5 38.0 32.5 38.0 40-41 35.508791669845145 38.0 36.0 38.0 31.0 38.0 42-43 35.96226828896178 38.0 36.5 38.0 32.5 38.0 44-45 36.14229727668014 38.0 37.0 38.0 33.5 38.0 46-47 36.42462239682661 38.0 37.0 38.0 34.0 38.0 48-49 36.212525745670916 38.0 37.0 38.0 34.0 38.0 50-51 36.353135250591194 38.0 37.0 38.0 34.0 38.0 52-53 36.29090701045084 38.0 37.0 38.0 34.0 38.0 54-55 36.48410443206957 38.0 37.0 38.0 34.0 38.0 56-57 36.526251048897706 38.0 37.0 38.0 34.0 38.0 58-59 36.57338469753604 38.0 37.0 38.0 34.0 38.0 60-61 36.25406209474407 38.0 37.0 38.0 33.5 38.0 62-63 36.51130902433442 38.0 37.0 38.0 34.0 38.0 64-65 36.50389999237166 38.0 37.0 38.0 34.0 38.0 66-67 35.90308185216264 38.0 37.0 38.0 32.0 38.0 68-69 35.28140018308032 38.0 36.0 38.0 29.0 38.0 70-71 35.20381989472881 38.0 36.0 38.0 29.0 38.0 72-73 34.814478602486844 38.0 36.0 38.0 26.0 38.0 74-75 35.38642344953848 38.0 36.5 38.0 30.5 38.0 76 34.82992600503471 38.0 36.0 38.0 27.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.33304275926207794 1101 2 0.3390748319890875 1101 3 0.07771593694864265 1101 4 0.3265862034945215 1101 5 0.2897066480493109 1101 6 -0.661728542824271 1101 7 0.22107151076343712 1101 8 0.5784858127140922 1101 9 0.9145139460807243 1101 10-11 0.5419750628352134 1101 12-13 0.9602082521234507 1101 14-15 0.8006810221916325 1101 16-17 0.49100521820881227 1101 18-19 0.0023218253244934317 1101 20-21 -0.2107642852645455 1101 22-23 0.5886945750446202 1101 24-25 -1.367987064720701 1101 26-27 -0.09259877480874934 1101 28-29 1.2133010328545595 1101 30-31 1.302522777414005 1101 32-33 0.4569176575856062 1101 34-35 0.1197704267018267 1101 36-37 0.10299554713766668 1101 38-39 0.28236166413446284 1101 40-41 1.3871062175824775 1101 42-43 0.959894075914292 1101 44-45 0.49282458770662885 1101 46-47 0.48249510663922024 1101 48-49 -1.2274449083463566 1101 50-51 -0.709924754023838 1101 52-53 -0.9752225794635407 1101 54-55 -0.41086883809032315 1101 56-57 -1.1695095693963964 1101 58-59 -1.5235860354100765 1101 60-61 -1.7388596950031001 1101 62-63 -0.8759307094654218 1101 64-65 -1.4513614050031123 1101 66-67 -1.7908277695775823 1101 68-69 -1.5223562547935003 1101 70-71 -2.039393586719804 1101 72-73 -1.9599160266783784 1101 74-75 -1.669233627201713 1101 76 -1.9258309060474943 1102 1 -0.9670294602853424 1102 2 0.2891229783540368 1102 3 0.33289167118071106 1102 4 0.08669694013390483 1102 5 0.23253283967184046 1102 6 0.24510824099855455 1102 7 -0.04553898514411969 1102 8 0.5423760390020078 1102 9 0.4789097092006074 1102 10-11 0.4945508933600067 1102 12-13 0.7257354548196489 1102 14-15 -0.09399880448145126 1102 16-17 0.07177074541151285 1102 18-19 -0.34096175676506135 1102 20-21 0.3394283001952516 1102 22-23 0.587310367052325 1102 24-25 -4.333381864913282 1102 26-27 -0.9715708499170788 1102 28-29 0.12380897700477789 1102 30-31 1.3742006782324907 1102 32-33 -0.07865046952079524 1102 34-35 0.6414964738852618 1102 36-37 -2.282897567932153 1102 38-39 -0.21240241867729281 1102 40-41 0.9920051102160841 1102 42-43 0.588625419197875 1102 44-45 0.9675477461081599 1102 46-47 0.9735880291235759 1102 48-49 -2.978889299294842 1102 50-51 -0.6076739114624488 1102 52-53 0.33405763238047115 1102 54-55 0.4446318841051493 1102 56-57 0.7970287069637365 1102 58-59 0.046707657416114046 1102 60-61 -4.627521226057503 1102 62-63 -2.2786750522675376 1102 64-65 -0.26894686480089547 1102 66-67 0.9978096882943461 1102 68-69 -0.3428906794444444 1102 70-71 -0.8455442848420986 1102 72-73 -6.096651703134803 1102 74-75 -1.9378301606634452 1102 76 -0.8746753932887046 1103 1 0.43276467249901174 1103 2 0.3317889587966505 1103 3 0.021208706692803503 1103 4 0.18793214342777986 1103 5 0.13737071923952016 1103 6 0.47953953281533046 1103 7 0.948490865602146 1103 8 0.8463389829031911 1103 9 0.22914130981821046 1103 10-11 0.6720084332467806 1103 12-13 0.16999690618352048 1103 14-15 0.3654196206343485 1103 16-17 0.43188397238011333 1103 18-19 0.6730114804969034 1103 20-21 -0.4295629504480729 1103 22-23 0.33393929139612055 1103 24-25 -2.8697946286806513 1103 26-27 -0.3173763053982981 1103 28-29 -0.16692699829115298 1103 30-31 1.1630066483817458 1103 32-33 1.1803047543597955 1103 34-35 -0.011514334143562621 1103 36-37 -0.74038598790127 1103 38-39 -0.023339114508480918 1103 40-41 -0.7831106900927196 1103 42-43 -0.3737266137408852 1103 44-45 1.145494220632095 1103 46-47 1.0114161299985085 1103 48-49 -2.4605928505043124 1103 50-51 -0.16734967059781525 1103 52-53 0.27430467303924644 1103 54-55 -0.0506074365330349 1103 56-57 0.31486195451906696 1103 58-59 -0.017857448090829564 1103 60-61 -3.933131107683856 1103 62-63 -0.8830497306000069 1103 64-65 0.30678653715484216 1103 66-67 -0.2610168685764691 1103 68-69 0.20489624798736372 1103 70-71 0.44611809292424454 1103 72-73 -5.173208573953126 1103 74-75 -2.9632547617957172 1103 76 -4.071381517838372 1104 1 0.15690260353349572 1104 2 0.22144413121043982 1104 3 0.021208706692803503 1104 4 0.02931145377260691 1104 5 -0.12469824627771686 1104 6 0.17609125695326355 1104 7 0.5415943138780079 1104 8 0.5911665691100865 1104 9 0.8360378615423443 1104 10-11 -0.6590260495118443 1104 12-13 0.3113762165283447 1104 14-15 0.517143758565382 1104 16-17 0.3594701792766628 1104 18-19 0.6764597563589732 1104 20-21 -0.3157698469997996 1104 22-23 0.037387567258193144 1104 24-25 0.49917088856073377 1104 26-27 1.0860719704637773 1104 28-29 1.2089350706743645 1104 30-31 0.8733514759679579 1104 32-33 0.5596150991873827 1104 34-35 0.243658079649542 1104 36-37 1.0734071155470062 1104 38-39 1.58355743721566 1104 40-41 1.8306824133555537 1104 42-43 0.48489407591429057 1104 44-45 0.6144597378734744 1104 46-47 0.7872781989640174 1104 48-49 -0.043351471193965097 1104 50-51 0.5326503294021876 1104 52-53 0.6087874316599269 1104 54-55 0.6011167013979986 1104 56-57 0.32865505796733885 1104 58-59 -0.5316505515391086 1104 60-61 0.7323861336954494 1104 62-63 0.6652261314689554 1104 64-65 0.5654072268100165 1104 66-67 0.0631210624580163 1104 68-69 1.6600686617804712 1104 70-71 1.6426698170621705 1104 72-73 1.3750672881158366 1104 74-75 0.5953659278594543 1104 76 0.11482537871335552 1105 1 0.7515487015371249 1105 2 0.38024630906888035 1105 3 0.18786024934253476 1105 4 -0.1326667676429949 1105 5 -0.7987908052613832 1105 6 -0.3482735343353056 1105 7 0.4248066550758338 1105 8 -0.5435430497646863 1105 9 0.6655750666240152 1105 10-11 0.30714908660431206 1105 12-13 -0.5942272317475172 1105 14-15 0.11657660793017044 1105 16-17 -0.032657769906634826 1105 18-19 0.17666393058765095 1105 20-21 0.12826372831780475 1105 22-23 0.3786988195267895 1105 24-25 0.3819068232249734 1105 26-27 -0.04845616020773491 1105 28-29 0.8437127476253536 1105 30-31 0.9452434904870088 1105 32-33 0.814515280675586 1105 34-35 -0.808179488408534 1105 36-37 -0.0015248263767659864 1105 38-39 -0.03958230870085089 1105 40-41 0.174988220978058 1105 42-43 -0.628921713559393 1105 44-45 0.19358859449778976 1105 46-47 0.7769560574032255 1105 48-49 -0.29153658916129643 1105 50-51 0.10957864156190311 1105 52-53 0.26979015398297435 1105 54-55 0.6975776814342964 1105 56-57 0.03210333382941144 1105 58-59 0.47492839582931623 1105 60-61 0.5326810520257581 1105 62-63 -0.2194154293295938 1105 64-65 0.4318092231802524 1105 66-67 0.6374404816957622 1105 68-69 0.05945613909444347 1105 70-71 0.520029163704649 1105 72-73 0.744668014068651 1105 74-75 -0.611190333483556 1105 76 0.3780740175518318 1106 1 -1.6200417709945718 1106 2 0.11497546773195921 1106 3 0.19780070266562433 1106 4 0.11790949052065969 1106 5 0.034526520900733715 1106 6 0.41762158667891214 1106 7 -1.0485894263686575 1106 8 0.4887754289137618 1106 9 -0.8320718792077173 1106 10-11 0.46780506048060744 1106 12-13 1.2438202135079592 1106 14-15 -0.005181939899259191 1106 16-17 0.44479612944027025 1106 18-19 0.4822488326237604 1106 20-21 0.29794774675806224 1106 22-23 0.3705111514514954 1106 24-25 -0.6634014748925807 1106 26-27 1.1273506012213872 1106 28-29 0.7575381414017741 1106 30-31 0.5162913199145933 1106 32-33 0.2671660682284127 1106 34-35 1.0711184370620757 1106 36-37 1.1042905789248039 1106 38-39 0.5014280639460864 1106 40-41 0.9577148825224313 1106 42-43 0.9863539299288959 1106 44-45 1.1522900927690216 1106 46-47 1.1507315088054497 1106 48-49 1.2583097419950562 1106 50-51 0.2792146384885186 1106 52-53 0.49431474099796446 1106 54-55 -0.14423945264177007 1106 56-57 0.18538800536225608 1106 58-59 0.4501012732784133 1106 60-61 0.7494261538313722 1106 62-63 0.9798498415117365 1106 64-65 -0.6843788290671569 1106 66-67 0.1769141659062896 1106 68-69 1.7083948636430364 1106 70-71 0.5505228248648422 1106 72-73 0.6154901423821357 1106 74-75 0.15602537915571446 1106 76 0.12096683353338733 1107 1 0.04200699011571629 1107 2 -0.3234772856321584 1107 3 0.22496193212090532 1107 4 -0.06616121805474506 1107 5 0.12221707157355866 1107 6 0.05519048285051298 1107 7 0.6791031105001082 1107 8 -0.2659293727149219 1107 9 0.04612465488037287 1107 10-11 0.19492656601712355 1107 12-13 -0.28852995283595106 1107 14-15 0.11843392980629375 1107 16-17 0.3685013779630353 1107 18-19 0.3883762470931984 1107 20-21 0.6288231626178344 1107 22-23 0.13239108590703097 1107 24-25 0.06851172834492303 1107 26-27 -0.24667492139641922 1107 28-29 0.6933591851477345 1107 30-31 0.6419182130075995 1107 32-33 0.849692509461839 1107 34-35 -0.4193726498836483 1107 36-37 1.055157056902857 1107 38-39 0.297420913640714 1107 40-41 0.47710512036939434 1107 42-43 0.9080233276149698 1107 44-45 0.9245230735525709 1107 46-47 0.7876300638479137 1107 48-49 1.0538805250528043 1107 50-51 0.7949304138497624 1107 52-53 0.771513211627088 1107 54-55 0.5868544447712125 1107 56-57 0.8960489120607065 1107 58-59 0.6724310811139489 1107 60-61 0.9401506187998407 1107 62-63 0.6837576820202074 1107 64-65 0.9977553384684654 1107 66-67 -0.1632218885154728 1107 68-69 0.9991491215505874 1107 70-71 0.789139439393864 1107 72-73 1.0027238679891681 1107 74-75 -1.28441357014664 1107 76 0.5453672506345342 1108 1 -0.21745518401568376 1108 2 -0.06182363088166909 1108 3 -0.20091580152288913 1108 4 0.304016383187097 1108 5 0.2148527835764824 1108 6 -0.3443438275179247 1108 7 0.09479959043436281 1108 8 -0.9120965644238197 1108 9 -0.6890257811329619 1108 10-11 0.26420931267283976 1108 12-13 0.6108902179169107 1108 14-15 0.5659981904098643 1108 16-17 -0.4415018179462038 1108 18-19 0.22547618774060396 1108 20-21 1.0715479035209086 1108 22-23 -0.2013303221192686 1108 24-25 0.6091454314906031 1108 26-27 -0.895992366495264 1108 28-29 -1.1473019115056857 1108 30-31 -0.05981214356456377 1108 32-33 0.36688193556784654 1108 34-35 0.7810336871478043 1108 36-37 0.906524449497482 1108 38-39 1.1565498000946235 1108 40-41 0.7068453386043458 1108 42-43 -0.7204750516024916 1108 44-45 0.2329739706899545 1108 46-47 0.6638114088691367 1108 48-49 0.6115895146889301 1108 50-51 0.6239949255604884 1108 52-53 -0.09026371390822163 1108 54-55 -0.376689362013245 1108 56-57 0.2502241391985365 1108 58-59 -0.04842907502904126 1108 60-61 0.3973710908812933 1108 62-63 0.846087043294915 1108 64-65 0.2440001451159617 1108 66-67 0.8346322867116598 1108 68-69 1.2418784280382766 1108 70-71 -0.6656384449142223 1108 72-73 0.25447020410751264 1108 74-75 -0.774803014514994 1108 76 1.3737469246517904 1109 1 -1.1474021068564078 1109 2 0.30370692138398425 1109 3 0.3924956122596299 1109 4 0.34213543393939005 1109 5 -0.03197793976431029 1109 6 -0.3212267507095774 1109 7 -0.3874568468161499 1109 8 0.41487628288065537 1109 9 0.8199008320178933 1109 10-11 0.9038768127824994 1109 12-13 0.5385342061609819 1109 14-15 0.7136053317003856 1109 16-17 0.5641129559275555 1109 18-19 0.6085309756512913 1109 20-21 0.9190059023804054 1109 22-23 0.15740108990863177 1109 24-25 0.2787291486463701 1109 26-27 0.41512313115794086 1109 28-29 -0.6581147377547651 1109 30-31 0.8891504392314218 1109 32-33 0.6123308981506455 1109 34-35 0.1301805047115252 1109 36-37 1.0196095045485833 1109 38-39 0.8989281832152116 1109 40-41 0.45876445076822137 1109 42-43 0.7384888471561197 1109 44-45 0.8518273285879232 1109 46-47 0.5533363463609149 1109 48-49 0.7486250895452713 1109 50-51 0.13866790884775781 1109 52-53 -0.35407035513294716 1109 54-55 0.013625153054526606 1109 56-57 0.15628634036535516 1109 58-59 -0.43687930970904176 1109 60-61 0.5341215405018502 1109 62-63 0.37370032574436607 1109 64-65 0.7727319957980683 1109 66-67 0.3926004404160963 1109 68-69 -0.7753606823709873 1109 70-71 0.1238733329512911 1109 72-73 0.21861022253098383 1109 74-75 0.18810877212360566 1109 76 0.3838359714562003 1110 1 -0.4664964112448331 1110 2 -0.32313714957773243 1110 3 -0.11721493862739152 1110 4 -0.0736442112520237 1110 5 0.34500618721981624 1110 6 -0.4029727824556062 1110 7 0.26277657988786274 1110 8 0.7446148449721548 1110 9 0.8702743147443144 1110 10-11 -1.0506516652753888 1110 12-13 0.22541562539534254 1110 14-15 0.6119713447722788 1110 16-17 0.11969185415351546 1110 18-19 -0.36910674610408023 1110 20-21 0.5475306456110332 1110 22-23 -0.5869966691950168 1110 24-25 -0.9757059587299111 1110 26-27 0.8184611330253517 1110 28-29 0.10696462732460787 1110 30-31 -0.144136208761104 1110 32-33 0.5767333257883678 1110 34-35 0.6537566018170367 1110 36-37 1.1891706623450347 1110 38-39 0.8382372401713241 1110 40-41 -1.7072486211271993 1110 42-43 -0.27582020979999555 1110 44-45 0.6826863388586943 1110 46-47 0.49171169650097113 1110 48-49 1.1210573958011025 1110 50-51 -0.607620606558406 1110 52-53 -0.013010597896716547 1110 54-55 -0.37573058924239433 1110 56-57 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36-37 0.8646035627779369 2116 38-39 -3.1406588637247808 2116 40-41 -0.8542183180969047 2116 42-43 -0.7105604695402548 2116 44-45 -0.1981578169854572 2116 46-47 -1.258829429040155 2116 48-49 0.08047298021669036 2116 50-51 -0.6064301303679258 2116 52-53 0.07670801682189676 2116 54-55 0.04526508175327848 2116 56-57 0.691194242920318 2116 58-59 -1.5089232788118352 2116 60-61 -1.2431363323442568 2116 62-63 0.35684055153164707 2116 64-65 -0.83503686932373 2116 66-67 0.10494446893659415 2116 68-69 0.4044521518536115 2116 70-71 1.6278840281384532 2116 72-73 1.2085834845630714 2116 74-75 1.0338455516839105 2116 76 0.26722872249600726 2117 1 0.9930969151368174 2117 2 0.4945375115275823 2117 3 0.38028245449044107 2117 4 0.15440584088562304 2117 5 0.27540243330949465 2117 6 0.5271106377738022 2117 7 0.7762280918795241 2117 8 0.42308199825682635 2117 9 -1.4525098354120942 2117 10-11 -1.0139467643369073 2117 12-13 -1.1393914653241595 2117 14-15 -0.18766369172406883 2117 16-17 -0.7778316077860126 2117 18-19 -0.8900139410988643 2117 20-21 0.0424732942033188 2117 22-23 0.045693633203313766 2117 24-25 -1.2838394310969647 2117 26-27 -1.5186348002384662 2117 28-29 -0.7242136834157478 2117 30-31 0.045488400206565416 2117 32-33 -2.024804734691294 2117 34-35 0.6112644224635417 2117 36-37 -0.9322057714401524 2117 38-39 -0.2832434689006291 2117 40-41 -0.25396394959435753 2117 42-43 0.2491276525566235 2117 44-45 -0.7783668415375544 2117 46-47 -0.7361298050631575 2117 48-49 -1.1650479222385144 2117 50-51 -0.4069167483727938 2117 52-53 0.49066510596146884 2117 54-55 -0.6186920073862865 2117 56-57 0.24013253090970466 2117 58-59 -1.0024534712471365 2117 60-61 -0.8637125323000134 2117 62-63 -0.4763545380503018 2117 64-65 -0.6186853984102214 2117 66-67 -0.6734507975973614 2117 68-69 0.3434313599934029 2117 70-71 -0.5115209707555977 2117 72-73 0.8600156898273852 2117 74-75 0.6560253791557145 2117 76 0.5589230379129475 2118 1 0.02897642577730153 2118 2 0.3809875989132081 2118 3 -1.073351759552942 2118 4 -0.14990175652364712 2118 5 0.17850719325603848 2118 6 0.22650407871139322 2118 7 0.7189134008134133 2118 8 0.3270092111693401 2118 9 -1.5887800112114192 2118 10-11 -0.4850581039071784 2118 12-13 -1.2183469500573736 2118 14-15 0.5676051475891768 2118 16-17 -2.705609956711683 2118 18-19 -0.7195091605910022 2118 20-21 -0.18395828799057767 2118 22-23 0.6750757654126289 2118 24-25 0.2999479648113308 2118 26-27 -2.187725989711069 2118 28-29 -0.46758265637760843 2118 30-31 -0.6158665910548748 2118 32-33 -0.22532904845709822 2118 34-35 -0.26022730160348573 2118 36-37 -4.095436983530213 2118 38-39 -0.5002939469514089 2118 40-41 -0.6079528464793142 2118 42-43 0.7151757660551397 2118 44-45 -2.7261667798535782 2118 46-47 0.13356280314080493 2118 48-49 0.17714391491579562 2118 50-51 -1.3376507876449253 2118 52-53 -1.747769149204565 2118 54-55 -0.2534971888399795 2118 56-57 0.20815967185757955 2118 58-59 -0.8168618191447408 2118 60-61 -1.1729805491603003 2118 62-63 -1.4339725086476136 2118 64-65 0.047218785819239883 2118 66-67 0.32832261661051376 2118 68-69 -1.667517545116084 2118 70-71 -0.16223547676978 2118 72-73 1.0993752045801415 2118 74-75 -0.022652527701488623 2118 76 0.24556845787799375 2119 1 0.6256088519130643 2119 2 -0.5934379014574347 2119 3 -0.13450817171009533 2119 4 -0.08943368493623183 2119 5 -0.1756705045095046 2119 6 -0.134927669673651 2119 7 -0.28008056296928174 2119 8 0.39536672467140477 2119 9 -0.008297113827111957 2119 10-11 0.22303254525093053 2119 12-13 2.27655470531829E-4 2119 14-15 0.3717457808625042 2119 16-17 0.09281215490538841 2119 18-19 -0.0672270468559546 2119 20-21 -1.2340483017573831 2119 22-23 -0.2242147143077915 2119 24-25 0.9791812593151974 2119 26-27 0.1836866969351263 2119 28-29 0.1656112438659605 2119 30-31 0.8818036408629482 2119 32-33 0.6960942280440037 2119 34-35 0.6556363010651509 2119 36-37 -0.24541580382037864 2119 38-39 -0.7670259177234087 2119 40-41 -1.1027373429317109 2119 42-43 0.3743677601248194 2119 44-45 0.0544908501368937 2119 46-47 0.24735079424532813 2119 48-49 0.2366588995605028 2119 50-51 0.6842966866746778 2119 52-53 0.3116134622536606 2119 54-55 -0.03738472458073261 2119 56-57 -1.0054906511329946 2119 58-59 -0.10307912296767796 2119 60-61 0.4523239091686193 2119 62-63 0.28669359322680066 2119 64-65 -0.23783363396260881 2119 66-67 0.6393201809438835 2119 68-69 0.4490238082673699 2119 70-71 -0.7873016633630243 2119 72-73 0.5623371870009848 2119 74-75 -0.18308882972414864 2119 76 0.8761943183037104 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 4.0 23 8.0 24 17.0 25 39.0 26 70.0 27 159.0 28 265.0 29 438.0 30 674.0 31 1165.0 32 1746.0 33 2825.0 34 4355.0 35 6850.0 36 11936.0 37 21885.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.46487146235411 24.160881836905943 12.239682660767413 29.13456403997254 2 16.788084522084066 20.087344572431153 39.73987336944084 23.384697536043937 3 17.367838889312686 25.326111831566102 31.487909070104507 25.818140209016704 4 13.025402395300938 20.018689449996184 37.930048058585704 29.025860096117174 5 12.581051186207947 36.928827523075746 37.216797619955756 13.273323670760545 6 27.116866275078188 39.921046609199784 18.06392554733389 14.89816156838813 7 25.423373255015637 30.439011366236933 23.522007780913874 20.61560759783355 8 21.74269585780761 37.81371576779312 20.947440689602566 19.496147684796703 9 22.600884888244718 16.275078190556105 23.48386604622778 37.640170874971396 10-11 23.79662827065375 26.86417728278282 27.583148981615686 21.756045464947746 12-13 23.571592036005796 23.29983217636738 27.3867190479823 25.74185673964452 14-15 21.547219467541385 25.541612632542527 23.173964451903274 29.737203448012817 16-17 22.691471508124188 28.70642306812114 25.188801586696165 23.41330383705851 18-19 22.342474635746434 27.44774582348005 26.63151270119765 23.578266839575864 20-21 20.857807613090245 26.816500114425203 26.422686703791285 25.903005568693267 22-23 21.967732092455563 28.59295140743001 26.044129987031813 23.395186513082614 24-25 22.29193683728736 29.19273018536883 26.38359142573804 22.131741551605767 26-27 21.221107635975283 29.168891601190023 27.87207262186284 21.737928140971853 28-29 21.075215500800976 26.518041040506525 29.509306583263406 22.897436875429094 30-31 21.116217865588528 26.457014265008773 30.859523991151118 21.56724387825158 32-33 21.710275383324433 27.252269433213822 27.10447021130521 23.932984972156536 34-35 21.123846212525745 26.701121366999768 29.316690823098636 22.858341597375848 36-37 21.12575329926005 29.313830192997177 26.812685940956595 22.747730566786178 38-39 21.01895644213899 26.94999618582653 28.47185139980166 23.559195972232814 40-41 20.32573041421924 25.82481501258677 30.472385384087268 23.37706918910672 42-43 22.36822030665955 25.100122053550994 29.941261728583417 22.59039591120604 44-45 19.75455793729499 26.26153787474254 29.96414676939507 24.019757418567398 46-47 21.72934625066748 23.916774734914945 30.254023953009384 24.099855061408192 48-49 20.104508353039897 24.905599206651917 30.322679075444352 24.667213364863834 50-51 18.790525593103975 24.98283621939126 31.388740559920663 24.837897627584102 52-53 18.14402319017469 25.326111831566102 33.017392631016854 23.51247234724235 54-55 17.136127851094667 25.681783507513924 31.46025631245709 25.72183232893432 56-57 18.853459455336026 25.365207109619348 29.823975894423675 25.957357540620947 58-59 17.42314440460752 26.07368983141353 31.36299488900755 25.140170874971396 60-61 19.085170493554045 27.51258677244641 28.521435654893583 24.880807079105956 62-63 17.45461133572355 29.663780608742087 27.150240292928522 25.731367762605846 64-65 17.930429475932566 30.09287512396064 25.895377221756043 26.081318178350756 66-67 17.700625524448853 30.424708215729652 25.031466931116025 26.84319932870547 68-69 17.982874361125944 29.766763292394536 25.045770081623314 27.204592264856203 70-71 18.244145243725683 28.802730948203525 25.167823632618813 27.785300175451976 72-73 18.458692501334962 28.216301777404833 25.423373255015637 27.901632466244564 74-75 18.6999389732245 27.86158364482417 25.863910290640018 27.574567091311312 76 18.76764055229232 27.50781905561065 26.838431611869705 26.886108780227325 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 1.0 14 2.0 15 10.0 16 12.5 17 7.0 18 7.0 19 11.0 20 18.5 21 22.0 22 29.5 23 57.0 24 68.0 25 59.0 26 112.0 27 181.5 28 239.0 29 280.0 30 344.5 31 496.0 32 721.5 33 860.0 34 957.0 35 1240.5 36 1443.0 37 1459.0 38 1771.0 39 2487.0 40 3298.0 41 3898.5 42 4092.0 43 4007.5 44 4008.5 45 4127.0 46 4160.0 47 4024.5 48 3832.5 49 3402.0 50 3028.0 51 2808.5 52 2317.5 53 1819.5 54 1593.0 55 1506.0 56 1179.0 57 796.5 58 654.0 59 510.5 60 303.0 61 189.0 62 139.0 63 101.5 64 42.0 65 22.0 66 16.5 67 9.0 68 7.0 69 4.0 70 3.0 71 3.0 72 1.5 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 52436.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.728125715157525 #Duplication Level Percentage of deduplicated Percentage of total 1 59.10417883059471 27.02723319856587 2 17.745433313871047 16.229308108932795 3 9.150054216365001 12.552444885193378 4 4.987905580115106 9.123502936913571 5 2.898490282759196 6.6271264017087494 6 1.843356410042539 5.057594019376001 7 1.16773709233464 3.737889999237165 8 0.8049044957878054 2.94454191776642 9 0.5713570773208775 2.351437943397666 >10 1.7015597631161898 10.57479594171943 >50 0.008340979230961715 0.24220001525669388 >100 0.012511468846442572 1.3349607140132733 >500 0.0 0.0 >1k 0.0041704896154808576 2.1969639179189873 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT 1152 2.1969639179189873 No Hit ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT 475 0.9058661987947211 RNA PCR Primer, Index 48 (95% over 23bp) CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC 122 0.23266458158517048 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG 103 0.19642993363338163 No Hit ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 64 0.12205355099549928 No Hit CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT 63 0.1201464642611946 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.005721260202914028 0.0 2 0.0 0.0 0.0 0.005721260202914028 0.0 3 0.0 0.0 0.0 0.005721260202914028 0.0 4 0.0 0.0 0.0 0.009535433671523381 0.0 5 0.0 0.0 0.0 0.009535433671523381 0.0 6 0.0 0.0 0.0 0.01525669387443741 0.0 7 0.0 0.0 0.0 0.01525669387443741 0.0 8 0.0 0.0 0.0 0.017163780608742085 0.0 9 0.0 0.0 0.0 0.038141734686093526 0.0 10 0.0 0.0 0.0 0.04958425509192158 0.0 11 0.0 0.0 0.0 0.06484094896635899 0.0 12 0.0 0.0 0.0 0.06674803570066366 0.0 13 0.0 0.0 0.0 0.07246929590357769 0.0 14 0.0 0.0 0.0 0.07246929590357769 0.0 15 0.0 0.0 0.0 0.07437638263788238 0.0 16 0.0 0.0 0.0 0.07628346937218705 0.0 17 0.0 0.0 0.0 0.0800976428407964 0.0 18 0.0 0.0 0.0 0.08391181630940575 0.0 19 0.0 0.0 0.0 0.09916851018384316 0.0 20 0.0 0.0 0.0 0.10870394385536654 0.0 21 0.0 0.0 0.0 0.11442520405828058 0.0 22 0.0 0.0 0.0 0.12205355099549928 0.0 23 0.0 0.0 0.0 0.1277748111984133 0.0 24 0.0 0.0 0.0 0.12968189793271798 0.0 25 0.0 0.0 0.0 0.14112441833854603 0.0 26 0.0 0.0 0.0 0.14112441833854603 0.0 27 0.0 0.0 0.0 0.17163780608742085 0.0 28 0.0 0.0 0.0 0.20596536730490503 0.0 29 0.0 0.0 0.0 0.25936379586543595 0.0 30 0.0 0.0 0.0 0.28987718361431075 0.0 31 0.0 0.0 0.0 0.3509039591120604 0.0 32 0.0 0.0 0.0 0.4176519948127241 0.0 33 0.0 0.0 0.0 0.47677168357616906 0.0 34 0.0 0.0 0.0 0.5377984590739187 0.0 35 0.0 0.0 0.0 0.6407811427263712 0.0 36 0.0 0.0 0.0 0.8867953314516744 0.0 37 0.0 0.0 0.0 1.3406819742161873 0.0 38 0.0 0.0 0.0 1.9623922495995119 0.0 39 0.0 0.0 0.0 2.6222442596689297 0.0 40 0.0 0.0 0.0 3.602486841101533 0.0 41 0.0 0.0 0.0 4.645663284766191 0.0 42 0.0 0.0 0.0 5.877641315127012 0.0 43 0.0 0.0 0.0 7.0695705240674345 0.0 44 0.0 0.0 0.0 8.314898161568388 0.0 45 0.0 0.0 0.0 9.544969105194903 0.0 46 0.0 0.0 0.0 10.673964451903272 0.0 47 0.0 0.0 0.0 11.78198184453429 0.0 48 0.0 0.0 0.0 12.710733084140667 0.0 49 0.0 0.0 0.0 13.729117400259364 0.0 50 0.0 0.0 0.0 14.562514303150508 0.0 51 0.0 0.0 0.0 15.714394690670531 0.0 52 0.0 0.0 0.0 16.81859790983294 0.0 53 0.0 0.0 0.0 17.91707986879243 0.0 54 0.0 0.0 0.0 19.055610649172323 0.0 55 0.0 0.0 0.0 20.150278434663207 0.0 56 0.0 0.0 0.0 21.050423373255015 0.0 57 0.0 0.0 0.0 21.8609352353345 0.0 58 0.0 0.0 0.0 22.684796704554124 0.0 59 0.0 0.0 0.0 23.440003051338774 0.0 60 0.0 0.0 0.0 24.117018842016936 0.0 61 0.0 0.0 0.0 24.84171180105271 0.0 62 0.0 0.0 0.0 25.453886642764513 0.0 63 0.0 0.0 0.0 26.294911892592875 0.0 64 0.0 0.0 0.0 27.137844229155544 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCAAG 15 0.002213041 70.0 21 TAACACG 15 0.002213041 70.0 4 CATACCA 15 0.002213041 70.0 19 AACACGA 15 0.002213041 70.0 5 GTACACT 20 0.0069152513 52.5 41 TGGTTCG 20 0.0069152513 52.5 17 GTTACGA 20 0.0069152513 52.5 54 TCCAGTA 20 0.0069152513 52.5 37 TGATTCG 20 0.0069152513 52.5 43 TCGTCCA 20 0.0069152513 52.5 21 TGCCTTC 20 0.0069152513 52.5 10 TTCGGGA 20 0.0069152513 52.5 46 ACCAAGG 20 0.0069152513 52.5 22 TGAATAA 20 0.0069152513 52.5 12 GATTCGG 20 0.0069152513 52.5 44 CGTCCAA 20 0.0069152513 52.5 22 ACATACC 20 0.0069152513 52.5 18 ATGTTAC 20 0.0069152513 52.5 52 GGTTAAG 20 0.0069152513 52.5 8 GCACTTT 20 0.0069152513 52.5 31 >>END_MODULE