FastQCFastQC Report
Wed 25 May 2016
SRR1780641_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780641_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655576
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAGT250.002337833235.24339313
TACGCAG250.002345741335.21914336
CTCCGGT401.838758E-532.947427
CCAACGT353.2117765E-431.4360522
CGCTAGA300.00568519529.39422618
ACGCTAG300.00568732329.39197717
CGTACTG300.00572787229.34928537
TTACGCA300.00572787229.34928535
CGACTTC300.00572787229.34928534
TCCGCGA300.005745013229.33134811
CACGTAG454.083474E-529.3156726
TATCGGC703.511377E-828.34660315
TTGTACG555.018359E-627.955399
GGCACTA406.953298E-427.55075821
ATAAGTC407.0063607E-427.51495440
GCACTAG407.0188945E-427.50654422
GACTTTA655.967395E-727.0544233
GTATCGG756.8730515E-826.45075614
ATCACGT508.28334E-526.42445824
GACATCG508.322207E-526.40628222
Produced by FastQC (version 0.11.5)