FastQCFastQC Report
Wed 25 May 2016
SRR1780641_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780641_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655576
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT158262.414060307271773No Hit
ATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTT55840.8517700464934653Illumina PCR Primer Index 3 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC21440.327040648223852No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG15400.23490792829511759No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC8910.13591101565646088No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTAA207.854069E-443.99992816
AAAACGG207.85702E-443.996579
CCGTCTT6750.037.8044644
GCTAGAT357.277884E-637.7199753
TTACGTA250.002350270335.2053073
CTGCGTA250.002352030235.1999415
GCCGTCT7500.034.02401443
TTCTGCG353.2163013E-431.42851613
ATGATTC2000.029.69768140
TGCCGTC8600.029.67210642
TATGGAA300.005738888429.3377574
ATTGGCG2100.029.33328221
TCTGCGT300.00574316929.3332814
CGTATTG300.00574316929.3332812
GACCGGA300.00574316929.3332811
GTCTCCG300.00574316929.3332813
TGTCTCG300.00574316929.3332810
GGGCGTC300.00574316929.3332822
TTGAAAG300.00574316929.3332819
TCGCGGA300.00574316929.3332819
Produced by FastQC (version 0.11.5)