FastQCFastQC Report
Wed 25 May 2016
SRR1780640_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780640_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53255
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTTTG150.00221429969.9915565
GTATCAT150.00221429969.9915557
ACTGGTG150.00221429969.991558
GTCATGA200.006919169352.493669
GATACTA200.006919169352.4936647
GGCAGAG200.006919169352.4936621
GAACTGC200.006919169352.4936619
TGTGGCA200.006919169352.4936625
TCTAGGG200.006919169352.493663
GTAATCA200.006919169352.4936624
GGCTTAT200.006919169352.4936612
ATTTTGC200.006919169352.4936666
CTGTTCT200.006919169352.4936670
GGAAATC200.006919169352.4936627
TAAGGTG200.006919169352.4936649
CGGTAGA200.006919169352.4936614
GCTAATG200.006919169352.4936611
ATTTCCT200.006919169352.4936636
AACAATA500.00715396227.9966210
ATGAATG500.00715396227.9966215
Produced by FastQC (version 0.11.5)