##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780640_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 53255 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.74627734485025 34.0 31.0 34.0 12.0 34.0 2 30.85610740775514 34.0 32.0 34.0 21.0 34.0 3 31.064069101492816 34.0 32.0 34.0 23.0 34.0 4 31.532363158388883 34.0 34.0 34.0 23.0 34.0 5 31.420486339310862 34.0 34.0 34.0 23.0 34.0 6 33.85453009107126 38.0 34.0 38.0 22.0 38.0 7 33.279541827058495 37.0 34.0 38.0 21.0 38.0 8 32.12430757675336 37.0 31.0 38.0 11.0 38.0 9 32.937301661815795 37.0 33.0 38.0 21.0 38.0 10-11 33.42252370669421 37.0 34.0 38.0 21.0 38.0 12-13 33.79757769223547 37.0 34.0 38.0 21.5 38.0 14-15 33.436606891371696 37.0 34.0 38.0 21.0 38.0 16-17 33.30410290113605 37.0 34.0 38.0 21.0 38.0 18-19 33.28516571213971 37.0 34.0 38.0 16.5 38.0 20-21 33.38487465965637 37.0 34.0 38.0 21.0 38.0 22-23 33.35485869871374 37.0 34.0 38.0 21.0 38.0 24-25 33.80674115106563 37.5 34.0 38.0 21.0 38.0 26-27 33.88141019622571 38.0 34.0 38.0 21.5 38.0 28-29 34.00491033705755 38.0 34.0 38.0 22.0 38.0 30-31 33.84082245798517 38.0 34.0 38.0 21.0 38.0 32-33 33.609370012205424 37.0 34.0 38.0 21.0 38.0 34-35 33.49923011923763 37.0 34.0 38.0 21.0 38.0 36-37 33.47110130504178 37.0 34.0 38.0 21.0 38.0 38-39 34.01473101117266 37.5 34.0 38.0 22.5 38.0 40-41 33.44388320345507 37.0 34.0 38.0 21.0 38.0 42-43 33.807642474884986 37.0 34.0 38.0 21.5 38.0 44-45 32.87642474884987 37.0 32.5 38.0 16.0 38.0 46-47 33.56507370199981 37.0 34.0 38.0 21.0 38.0 48-49 33.319303351797956 37.0 34.0 38.0 21.0 38.0 50-51 33.259769035771285 37.0 34.0 38.0 21.0 38.0 52-53 33.26874471880575 37.0 34.0 38.0 21.0 38.0 54-55 32.92533095483992 37.0 32.5 38.0 21.0 38.0 56-57 33.05151628955028 37.0 33.0 38.0 20.0 38.0 58-59 32.914777955121586 37.0 32.0 38.0 16.0 38.0 60-61 32.97969204769505 37.0 32.5 38.0 20.0 38.0 62-63 33.05812599755892 37.0 33.0 38.0 20.0 38.0 64-65 33.04822082433574 37.0 32.5 38.0 20.0 38.0 66-67 33.00107971082527 37.0 32.5 38.0 19.5 38.0 68-69 32.57593653178105 37.0 31.0 38.0 15.0 38.0 70-71 32.439329640409355 37.0 31.0 38.0 15.0 38.0 72-73 32.43744249366257 37.0 31.0 38.0 15.0 38.0 74-75 32.02612900197165 37.0 30.5 38.0 11.0 38.0 76 31.130166181579195 36.0 27.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.2066830551097354 1101 2 -0.4314592303114857 1101 3 0.04305864094670753 1101 4 -1.2788930619989465 1101 5 -0.39304799688888536 1101 6 -3.0040792450378326 1101 7 -1.158425670398124 1101 8 -0.9898923395478256 1101 9 -1.9444879064343787 1101 10-11 -1.3546028911525738 1101 12-13 -1.255147101925381 1101 14-15 -0.4797165697479926 1101 16-17 -2.0806575807675642 1101 18-19 -0.08830569183040637 1101 20-21 -0.5299344960684849 1101 22-23 -0.2612160083415134 1101 24-25 -2.020590824248366 1101 26-27 -1.1450108205833445 1101 28-29 -0.11414761557442432 1101 30-31 -1.3910695836094007 1101 32-33 -0.06244763478640181 1101 34-35 -0.9168499560384049 1101 36-37 0.11485687012556411 1101 38-39 -0.9632281634351472 1101 40-41 0.4905907738094655 1101 42-43 -0.4864170294671908 1101 44-45 -0.12357379135595892 1101 46-47 -0.11311997758843972 1101 48-49 -1.3080810220999481 1101 50-51 -0.5522527514349562 1101 52-53 -0.34463250689280045 1101 54-55 -1.2159208171047808 1101 56-57 -1.3749958629320531 1101 58-59 -1.1548786234388793 1101 60-61 -0.040459295407636375 1101 62-63 -1.3542195466601221 1101 64-65 -0.20433805808096395 1101 66-67 -1.2287070157823408 1101 68-69 -0.2796059247202649 1101 70-71 0.3668893616733371 1101 72-73 -1.1411592587186448 1101 74-75 -1.7696698906240869 1101 76 -1.6370353259439057 1102 1 -0.470757509831671 1102 2 -0.18325779146256593 1102 3 0.32666033102321634 1102 4 0.3029271960803044 1102 5 0.6032406864501567 1102 6 1.104062812744516 1102 7 1.818278680406948 1102 8 1.8908316526869555 1102 9 0.7687700586221453 1102 10-11 0.46968053924596376 1102 12-13 -3.0330676226516537 1102 14-15 -1.5740982072951013 1102 16-17 -2.012740485872051 1102 18-19 -0.4435928906427904 1102 20-21 -0.3319785750909787 1102 22-23 -0.9535478571481733 1102 24-25 0.17733654813943645 1102 26-27 -5.506778549257547 1102 28-29 0.04846515135488971 1102 30-31 0.8359488016823633 1102 32-33 -6.129279883273327 1102 34-35 -0.6191167140605671 1102 36-37 0.5006625113268868 1102 38-39 0.20272478848903575 1102 40-41 -0.037329746916803686 1102 42-43 -0.1857718313399772 1102 44-45 -5.734884742594971 1102 46-47 -0.2725067538816859 1102 48-49 0.035643997884058365 1102 50-51 -6.286236992613443 1102 52-53 -2.355281130850777 1102 54-55 0.4543429718640368 1102 56-57 -1.6377570825278056 1102 58-59 0.9269701832300683 1102 60-61 1.1666892714531656 1102 62-63 -4.835919900662635 1102 64-65 -0.7428215569961196 1102 66-67 -0.4631765829857173 1102 68-69 -6.952553281120856 1102 70-71 0.2238323786883072 1102 72-73 0.7276885202010845 1102 74-75 -6.064662467990765 1102 76 -0.8192324254071934 1103 1 0.16257906415238566 1103 2 0.16313536428310726 1103 3 0.7027540506421168 1103 4 0.8766195935137127 1103 5 0.9794316255907347 1103 6 -0.17471416567275355 1103 7 0.9963362343637812 1103 8 2.122505772850289 1103 9 1.0140063020598333 1103 10-11 1.4588891723394894 1103 12-13 -0.09604156781984585 1103 14-15 0.47604276101133536 1103 16-17 0.037424787314797925 1103 18-19 0.3075008289761172 1103 20-21 0.2158291246951407 1103 22-23 0.6129259908004911 1103 24-25 1.6578197291621848 1103 26-27 0.17110887053634372 1103 28-29 0.9917618653350502 1103 30-31 0.42349498095516225 1103 32-33 -4.296789119127887 1103 34-35 -0.7079267471151951 1103 36-37 0.9931279983966874 1103 38-39 1.566909116702135 1103 40-41 1.4064530646717586 1103 42-43 1.193870400820245 1103 44-45 -3.777102319884488 1103 46-47 0.7398401253716713 1103 48-49 0.36271477369584915 1103 50-51 0.4828180336358301 1103 52-53 0.2488895866292964 1103 54-55 0.977772876588908 1103 56-57 1.606020868084677 1103 58-59 1.1119060183457634 1103 60-61 1.40650552655719 1103 62-63 0.9576101401695638 1103 64-65 1.6692676905247907 1103 66-67 1.1039291918538652 1103 68-69 -3.098406868521206 1103 70-71 1.3437769635609484 1103 72-73 1.9929033753439924 1103 74-75 -1.563374309328502 1103 76 0.37379693488835386 1104 1 -1.4232363430915953 1104 2 1.1637788649266092 1104 3 0.8786595479761843 1104 4 0.8390207702006016 1104 5 0.19311980356462755 1104 6 -0.19002028812172966 1104 7 1.841574329601876 1104 8 1.6416269348285937 1104 9 0.49088624322548924 1104 10-11 -0.1261448412659476 1104 12-13 -0.3112695509049743 1104 14-15 0.0440929540615258 1104 16-17 -0.8476643834886559 1104 18-19 0.9315355780108661 1104 20-21 1.4893398803487585 1104 22-23 0.09649374222538398 1104 24-25 1.008320740377485 1104 26-27 0.8481864583282146 1104 28-29 -0.4667553253249963 1104 30-31 -1.230071851183105 1104 32-33 -0.2670961608635025 1104 34-35 0.4136489101747429 1104 36-37 0.4935416773817991 1104 38-39 0.1382457594673525 1104 40-41 0.8749445153060691 1104 42-43 1.0039457823241946 1104 44-45 -2.9087211836462075 1104 46-47 0.8239548523435332 1104 48-49 1.1499688645213624 1104 50-51 1.5055703778167455 1104 52-53 0.24323597383282447 1104 54-55 0.0799975502421546 1104 56-57 1.2644599193808688 1104 58-59 1.5656429185112373 1104 60-61 0.6613547635492836 1104 62-63 1.3492951925689027 1104 64-65 0.32060525257663386 1104 66-67 -0.03426257133789079 1104 68-69 -3.7104449269878366 1104 70-71 -0.08945984467586499 1104 72-73 0.5538520791498343 1104 74-75 -0.1954068520753296 1104 76 0.44573111396539034 1105 1 -0.40180135400161987 1105 2 -0.11384916741840456 1105 3 -0.809442107780459 1105 4 -0.14352319078001585 1105 5 -0.26291921581670863 1105 6 0.10044306703164807 1105 7 1.0859588758732173 1105 8 0.6859087985174597 1105 9 0.27114551674621623 1105 10-11 0.16014703397470953 1105 12-13 -1.4103582429855734 1105 14-15 -0.7065809003859158 1105 16-17 0.7304385558002693 1105 18-19 -0.26522992064143125 1105 20-21 -0.1160381199079481 1105 22-23 0.534755839517139 1105 24-25 0.5744226528405818 1105 26-27 0.09353365381018364 1105 28-29 -0.7760224283926362 1105 30-31 0.33744194678892114 1105 32-33 1.0993523053154277 1105 34-35 0.4896340212002883 1105 36-37 0.39143668444123136 1105 38-39 0.15903898241690229 1105 40-41 0.1231154818058755 1105 42-43 0.45555661533664704 1105 44-45 1.2123800870507537 1105 46-47 0.5196293485571175 1105 48-49 -0.250429030485698 1105 50-51 -0.025298573537384073 1105 52-53 -0.7848092065034606 1105 54-55 -0.7756692447777382 1105 56-57 0.19598347219822188 1105 58-59 0.09497163112835949 1105 60-61 0.07259850632941323 1105 62-63 1.084309953190136 1105 64-65 0.2559794022364912 1105 66-67 0.5910743558858229 1105 68-69 1.3523198015450788 1105 70-71 1.331474566824589 1105 72-73 -0.22441771933559096 1105 74-75 -1.0445276324629589 1105 76 -1.7288292762284208 1106 1 0.5238207406114519 1106 2 -0.3807345926303256 1106 3 -1.9379627531181676 1106 4 -1.0238896113433213 1106 5 0.08900859386335469 1106 6 -0.4826851801655039 1106 7 0.217350727117406 1106 8 0.8116949620394749 1106 9 1.6517853854910882 1106 10-11 0.0023674332090521943 1106 12-13 0.5352255688820762 1106 14-15 0.12483829567644023 1106 16-17 0.9716198519446451 1106 18-19 -0.2190855400015579 1106 20-21 -0.011991086820721364 1106 22-23 0.4342636327903122 1106 24-25 0.5435913703686168 1106 26-27 0.47765998687007283 1106 28-29 -1.178674875753856 1106 30-31 -0.8246444628364529 1106 32-33 0.7296503638333363 1106 34-35 0.626973370393614 1106 36-37 0.25478095570181836 1106 38-39 0.3666545142736197 1106 40-41 -0.24802206262945248 1106 42-43 0.8809052617681488 1106 44-45 0.8933344212044219 1106 46-47 0.4619317822459337 1106 48-49 0.11462429190971335 1106 50-51 0.8447451781716708 1106 52-53 -1.0866959989562872 1106 54-55 0.2406009220256493 1106 56-57 0.9314223565296444 1106 58-59 -0.05843279873218421 1106 60-61 1.3313488481396405 1106 62-63 0.8545451186262838 1106 64-65 0.9190228804973657 1106 66-67 1.073915482492339 1106 68-69 1.6755355521603406 1106 70-71 0.9245153106036383 1106 72-73 1.091414930377283 1106 74-75 -1.2922716849650087 1106 76 -1.5700926068272736 1107 1 -0.6015369627024967 1107 2 -0.084325472349704 1107 3 0.11853129285248087 1107 4 -0.25877376162486954 1107 5 -0.01871779771792248 1107 6 0.09439411458202329 1107 7 0.026591011433843903 1107 8 0.9454529238229128 1107 9 -1.1637781703106285 1107 10-11 -0.26404076396624276 1107 12-13 0.31317258263634784 1107 14-15 -0.6292464453925248 1107 16-17 1.0945774814101696 1107 18-19 0.816430937428521 1107 20-21 0.5384104639962217 1107 22-23 -1.0097500291685222 1107 24-25 -0.13016012967754875 1107 26-27 0.28083085366597516 1107 28-29 -0.2750009165551148 1107 30-31 -0.16141636351031963 1107 32-33 1.3542695828862321 1107 34-35 0.5000694656580862 1107 36-37 -0.4912496913950122 1107 38-39 0.669594604737938 1107 40-41 0.7515856176068567 1107 42-43 0.3261651159175045 1107 44-45 0.8606795702859316 1107 46-47 0.3972682541373729 1107 48-49 0.053560874573797435 1107 50-51 0.48217248362721676 1107 52-53 0.7041744893664301 1107 54-55 0.46029998968927544 1107 56-57 -0.009360436787076765 1107 58-59 0.858441566632905 1107 60-61 -0.47989919026154126 1107 62-63 -0.4608568937433333 1107 64-65 -0.11875520498219316 1107 66-67 1.2588827122532535 1107 68-69 1.2947546050542798 1107 70-71 -0.48315107913142796 1107 72-73 0.3742082470947281 1107 74-75 -0.06541855102242522 1107 76 0.22622420292071865 1108 1 0.1781061806728772 1108 2 -0.4546380382585049 1108 3 0.16252673723599642 1108 4 -0.2056249026380712 1108 5 -0.14796232465089787 1108 6 0.6249547084994305 1108 7 0.3092501416485476 1108 8 1.3812313858805325 1108 9 -0.3257556453180115 1108 10-11 0.42246533127988783 1108 12-13 0.3537302238393636 1108 14-15 0.7971676938912395 1108 16-17 -0.9968512105373506 1108 18-19 0.05121391292098565 1108 20-21 -1.8573122037679468E-4 1108 22-23 -0.36226194548016366 1108 24-25 -0.6870209724665983 1108 26-27 -0.13744223023329738 1108 28-29 0.675245846004465 1108 30-31 0.19649480196822822 1108 32-33 0.6231142279277861 1108 34-35 0.38402779020357514 1108 36-37 0.6839728167249532 1108 38-39 0.7757868686262483 1108 40-41 0.3130442585146085 1108 42-43 0.48696150516963144 1108 44-45 -0.15760616810356964 1108 46-47 0.07696201547368986 1108 48-49 0.41782037598688504 1108 50-51 -0.10018715698524971 1108 52-53 0.27202364784282906 1108 54-55 0.5865074389658531 1108 56-57 1.312169069265991 1108 58-59 -0.6437096967070346 1108 60-61 1.1224756357392138 1108 62-63 0.4593776361423565 1108 64-65 0.9259573271813082 1108 66-67 -0.005722678559592964 1108 68-69 1.1029758055435863 1108 70-71 1.9434950591399698 1108 72-73 1.4804637834342032 1108 74-75 1.3222306665082613 1108 76 2.3888765840739623 1109 1 -0.6339484760460437 1109 2 -0.5453832809443959 1109 3 -0.06041734860523107 1109 4 -0.12799894907951526 1109 5 0.13688971659313154 1109 6 0.011994695086741558 1109 7 -1.9688536390539397 1109 8 -0.24526706327698022 1109 9 0.9189840646325322 1109 10-11 0.873446134364805 1109 12-13 -0.7265153960784829 1109 14-15 -0.8342976426834952 1109 16-17 -1.7468721678682293 1109 18-19 -0.7642148740686991 1109 20-21 -0.15480891841125555 1109 22-23 -0.23735625346909472 1109 24-25 0.6848039919059445 1109 26-27 0.24513384487777046 1109 28-29 -0.6821604747265297 1109 30-31 -0.10661107446734519 1109 32-33 0.7623966489186387 1109 34-35 -0.9782825459520126 1109 36-37 -0.5423663567179204 1109 38-39 -0.3784381292780239 1109 40-41 -0.5440686595841413 1109 42-43 -0.5201220726658988 1109 44-45 -1.0652052962786058 1109 46-47 -1.2705943597463616 1109 48-49 -0.911104932085884 1109 50-51 0.10657786942098113 1109 52-53 -0.030788825960506472 1109 54-55 0.06150534323276702 1109 56-57 0.030670200961857574 1109 58-59 -0.8121018502823603 1109 60-61 0.12088287859277358 1109 62-63 -1.6074343346850384 1109 64-65 -0.4539362095778472 1109 66-67 0.006725975979762211 1109 68-69 0.7287826627822511 1109 70-71 -0.043197035754822366 1109 72-73 -0.19440418538128768 1109 74-75 1.171358841543892 1109 76 -0.13723625019546404 1110 1 0.8556752215342556 1110 2 0.4562958717293313 1110 3 0.1779792758673402 1110 4 -0.06574113456130348 1110 5 -0.2558597882721081 1110 6 0.36100012004153115 1110 7 0.8687852139556185 1110 8 2.2470691116993393 1110 9 -0.35945389282893814 1110 10-11 -0.46968225623194115 1110 12-13 -0.2092287345784385 1110 14-15 0.9556575799118718 1110 16-17 0.472063507667805 1110 18-19 1.1050049657659642 1110 20-21 0.30906777150521947 1110 22-23 0.5794869395042994 1110 24-25 0.26682414173802727 1110 26-27 -1.665418983848653 1110 28-29 0.21011542297432584 1110 30-31 0.3005403937148614 1110 32-33 1.0356249275718739 1110 34-35 0.9374584339842613 1110 36-37 -0.14591410493112278 1110 38-39 0.9511709295353796 1110 40-41 0.6742642431972214 1110 42-43 0.5889797959296388 1110 44-45 1.111686979619094 1110 46-47 0.9123902264931942 1110 48-49 1.5547307692832746 1110 50-51 0.36611459550381653 1110 52-53 -0.19894089691547379 1110 54-55 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44-45 2.059592457599763 2116 46-47 -0.12502473949319892 2116 48-49 0.23034838475050634 2116 50-51 -0.13997204968057275 2116 52-53 0.8957601413945895 2116 54-55 -0.19211129329526244 2116 56-57 -0.8574520032829298 2116 58-59 -0.6588468774071288 2116 60-61 -1.9134751684235063 2116 62-63 0.7671254825796439 2116 64-65 0.2497137380760961 2116 66-67 -0.4164680851223608 2116 68-69 0.06667644787789584 2116 70-71 -2.4140212481846355 2116 72-73 1.1537446677642293 2116 74-75 -0.763613081935695 2116 76 -0.238121374398375 2117 1 -0.5310667092806227 2117 2 0.557902471816174 2117 3 0.49712821203755553 2117 4 -0.15663706740252437 2117 5 -1.524929118131709 2117 6 -2.2438631018429653 2117 7 -2.0976353968418913 2117 8 -2.0606454634924383 2117 9 0.338186865602097 2117 10-11 0.4766196687933899 2117 12-13 0.6240322293816121 2117 14-15 -0.853576752118812 2117 16-17 0.6029797039335492 2117 18-19 0.40063385561977327 2117 20-21 -0.5469895450384215 2117 22-23 0.5355587299225917 2117 24-25 1.0029654067546758 2117 26-27 1.2009871530742018 2117 28-29 0.6632924384613688 2117 30-31 0.24604625563409144 2117 32-33 1.2745162287732086 2117 34-35 0.17577078019934333 2117 36-37 -1.0393140180878788 2117 38-39 0.29318858766245626 2117 40-41 -0.9830667743161641 2117 42-43 -1.2311823114665117 2117 44-45 0.7517217169182757 2117 46-47 -0.22431551963504148 2117 48-49 -0.17569331524415333 2117 50-51 1.3758120915235068 2117 52-53 0.8140132209018631 2117 54-55 -0.4783321646225147 2117 56-57 -0.6277820838033819 2117 58-59 0.11825545288668593 2117 60-61 1.1741641426200644 2117 62-63 -0.49597908735582763 2117 64-65 -0.4638787537918745 2117 66-67 -1.1421653069002033 2117 68-69 0.12296077571878072 2117 70-71 1.1572473488398387 2117 72-73 1.0582666109199934 2117 74-75 0.43051585743400267 2117 76 0.19663573113140842 2118 1 -0.21551381746162335 2118 2 -0.1004733148185295 2118 3 -1.042678001127964 2118 4 0.15716774730130112 2118 5 -0.4679418041770589 2118 6 0.279511186439926 2118 7 1.112533417462373 2118 8 -3.899572643594329 2118 9 -0.861992936615465 2118 10-11 -0.6925192783647347 2118 12-13 0.6128944809049344 2118 14-15 -1.3336736455360523 2118 16-17 -0.4178473862672334 2118 18-19 -0.591100236878475 2118 20-21 -2.458561815540868 2118 22-23 -0.4539278348561524 2118 24-25 -0.26371624343012456 2118 26-27 0.6618302256943167 2118 28-29 -0.6519857555252457 2118 30-31 -0.6218990045796673 2118 32-33 -0.7708089135702139 2118 34-35 0.9450995185871207 2118 36-37 -0.12402084058312823 2118 38-39 -0.08859145429138948 2118 40-41 -1.2962972208250036 2118 42-43 -0.16185856758477968 2118 44-45 -0.03476314972841976 2118 46-47 -0.7482641761129187 2118 48-49 -0.8988478452611375 2118 50-51 -0.6962836032122866 2118 52-53 -1.3179166562332867 2118 54-55 0.1760979622379395 2118 56-57 0.9070296233187847 2118 58-59 1.1027225390918645 2118 60-61 -1.1232337197313527 2118 62-63 0.1439919445091178 2118 64-65 -0.2217201282799408 2118 66-67 -2.025543590787926 2118 68-69 1.5085116697353556 2118 70-71 -1.3401305354874005 2118 72-73 -1.473598337636595 2118 74-75 0.5566770800890879 2118 76 0.5177824697189592 2119 1 -0.006020637911884563 2119 2 0.34413132086961795 2119 3 0.31708688741589697 2119 4 0.9941978016431001 2119 5 1.2444348177592843 2119 6 1.7681992201540169 2119 7 -2.694608647525982 2119 8 0.5833485053465637 2119 9 -1.1109670965356173 2119 10-11 0.10849547155208938 2119 12-13 0.5070388748275292 2119 14-15 -0.0863907354772806 2119 16-17 0.11797384030480629 2119 18-19 -0.4137962555099506 2119 20-21 -1.222535420952866 2119 22-23 1.2646870037819795 2119 24-25 0.27590336397810233 2119 26-27 0.9477900793041698 2119 28-29 1.4922140892124816 2119 30-31 0.7773468643345964 2119 32-33 0.46433026797575394 2119 34-35 1.0858059619718432 2119 36-37 -0.6621710013904973 2119 38-39 -0.010128550886712162 2119 40-41 0.34529643560174605 2119 42-43 -0.143116192071858 2119 44-45 -1.0944836776201754 2119 46-47 0.3395421896406603 2119 48-49 -0.5093058689940904 2119 50-51 0.9413730453404483 2119 52-53 -1.1759348441006452 2119 54-55 -1.6476793472885092 2119 56-57 1.1501259968354702 2119 58-59 1.7708494105568704 2119 60-61 0.6562661139162032 2119 62-63 -0.6437146097772342 2119 64-65 0.7848507933126143 2119 66-67 0.051976648759591626 2119 68-69 -1.357158730619524 2119 70-71 -1.8365539579117112 2119 72-73 -1.7815167140367265 2119 74-75 -0.02466926570220096 2119 76 0.5947482011152125 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 17.0 13 86.0 14 221.0 15 401.0 16 514.0 17 583.0 18 630.0 19 605.0 20 530.0 21 528.0 22 518.0 23 582.0 24 577.0 25 649.0 26 792.0 27 918.0 28 1054.0 29 1294.0 30 1618.0 31 1981.0 32 2486.0 33 3350.0 34 4461.0 35 6662.0 36 10796.0 37 11399.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.543956563688305 26.633118295843662 11.350952534100154 28.471972606367885 2 16.389071448690267 20.362407285700872 37.010609332457044 26.237911933151814 3 17.083841892780022 25.986292366913904 29.570932306825647 27.358933433480427 4 12.415735611679654 19.528682752793163 39.11182048633931 28.943761149187868 5 12.5715895221106 36.8716552436391 36.03041967890339 14.526335555346916 6 25.319688292179137 38.486527086658526 17.934466247300723 18.25931837386161 7 24.31321002722749 30.397145807905364 24.645573185616374 20.644070979250774 8 21.393296404093512 34.61459017932588 19.66200356774012 24.330109848840483 9 22.702093700122052 16.325227678152284 22.34156417237818 38.63111444934748 10-11 23.876631302225142 25.876443526429444 27.032203548962542 23.214721622382875 12-13 22.719932400713546 23.778049009482675 26.420993333959252 27.08102525584452 14-15 20.890996150596187 25.457703502018592 23.817481926579664 29.833818420805557 16-17 21.815791944418365 27.930710731386725 25.39667636841611 24.8568209557788 18-19 21.261853347103557 26.65571307858417 26.401276875410762 25.681156698901514 20-21 20.506055769411322 27.56267017181485 26.45760961412074 25.47366444465308 22-23 22.23077645291522 27.043470096704535 26.328983194066286 24.39677025631396 24-25 21.45244577973899 28.08374800488217 26.237911933151814 24.22589428222702 26-27 20.51262792226082 28.298751290958595 26.900760491972587 24.287860294808 28-29 21.546333677588958 27.155196695145996 28.09125903671017 23.207210590554876 30-31 21.62801614871843 27.68472443901981 27.311989484555443 23.37526992770632 32-33 20.14083184677495 28.388883672894565 27.570181203642854 23.900103276687634 34-35 21.700309830062906 27.996432259881704 27.049103370575533 23.25415453947986 36-37 21.852408224579854 26.80875035207962 27.08853628767252 24.25030513566801 38-39 21.280630926673552 27.268801051544457 27.95042718993522 23.500140831846775 40-41 21.328513754577035 26.698901511595157 27.793634400525775 24.178950333302037 42-43 20.586799361562296 26.368416111163274 28.401089099615064 24.643695427659374 44-45 19.812224204300065 26.378743779926765 28.54285982536851 25.266172190404657 46-47 20.34644634306638 26.589052671110693 28.677119519293964 24.387381466528964 48-49 19.904234344193032 26.37592714299127 29.191625199511783 24.528213313303915 50-51 19.822551873063563 26.33837198385128 28.2921791381091 25.54689700497606 52-53 19.23387475354427 26.48765374143273 28.8517510092949 25.4267204957281 54-55 18.65646418176697 27.02750915407004 28.66491409257347 25.651112571589525 56-57 18.878039620692892 27.11764153600601 28.43676650079805 25.567552342503053 58-59 19.389728663975212 27.042531217726033 27.7081964134823 25.859543704816453 60-61 19.308046192845744 26.142146277344853 28.067787062247678 26.48202046756173 62-63 19.436672612900196 26.109285513097362 28.31752887052859 26.136513003473855 64-65 19.83194066284856 26.308327856539293 27.156135574124495 26.703595906487653 66-67 20.60463806215379 26.500798047131724 26.214439958689322 26.68012393202516 68-69 19.59252652333114 26.867899727725096 26.506431321002722 27.033142427941037 70-71 20.501361374518826 26.469815040841237 26.14684067223735 26.88198291240259 72-73 21.00460050699465 25.897098863956437 26.03229743686039 27.066003192188525 74-75 20.97549525866116 25.559102431696555 26.142146277344853 27.323256032297436 76 21.068444277532624 24.587362688949394 26.953337714768566 27.390855318749413 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 0.5 15 0.5 16 0.5 17 1.0 18 3.0 19 4.0 20 7.5 21 12.0 22 16.0 23 28.0 24 41.0 25 46.0 26 77.5 27 112.0 28 172.0 29 229.0 30 303.5 31 409.0 32 603.5 33 767.0 34 898.5 35 1177.5 36 1432.0 37 1539.0 38 1781.5 39 2244.0 40 2821.0 41 3255.0 42 3332.0 43 3477.5 44 3797.0 45 4010.0 46 4049.0 47 4045.5 48 3950.5 49 3611.0 50 3363.0 51 3155.5 52 2707.5 53 2252.0 54 2037.0 55 1844.0 56 1444.5 57 1095.5 58 953.0 59 809.5 60 518.0 61 323.0 62 276.0 63 229.5 64 155.5 65 111.5 66 71.0 67 47.0 68 47.5 69 47.0 70 41.0 71 36.0 72 28.5 73 19.5 74 15.5 75 13.0 76 11.0 77 7.5 78 7.0 79 8.0 80 6.0 81 3.0 82 3.5 83 5.0 84 4.5 85 3.5 86 1.5 87 0.0 88 1.0 89 1.0 90 0.0 91 0.5 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.5 99 3.5 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7435921509717397 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 53255.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.74753544268144 #Duplication Level Percentage of deduplicated Percentage of total 1 75.87099661190308 44.5723406252934 2 9.74876941763089 11.454323537695991 3 5.452918238189606 9.610365223922637 4 3.062072492488653 7.195568491221481 5 1.7963306271175605 5.276499859168153 6 1.153870740906476 4.067223734860576 7 0.7575273285175478 3.1152004506619098 8 0.5273924439046219 2.4786405032391325 9 0.3867544588633894 2.0448784151722843 >10 1.2433676404781693 10.184959158764435 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.005633273870998028 0.0 2 0.0 0.0 0.0 0.0131443056989954 0.0 3 0.0 0.0 0.0 0.016899821612994085 0.0 4 0.0 0.0 0.0 0.02065533752699277 0.0 5 0.0 0.0 0.0 0.02065533752699277 0.0 6 0.0 0.0 0.0 0.02065533752699277 0.0 7 0.0 0.0 0.0 0.02065533752699277 0.0 8 0.0 0.0 0.0 0.02065533752699277 0.0 9 0.0 0.0 0.0 0.024410853440991455 0.0 10 0.0 0.0 0.0 0.045066190967984227 0.0 11 0.0 0.0 0.0 0.058210496666979625 0.0 12 0.0 0.0 0.0 0.06384377053797766 0.0 13 0.0 0.0 0.0 0.06947704440897569 0.0 14 0.0 0.0 0.0 0.0751103182799737 0.0 15 0.0 0.0 0.0 0.09013238193596845 0.0 16 0.0 0.0 0.0 0.10703220354896253 0.0 17 0.0 0.0 0.0 0.11454323537695991 0.0 18 0.0 0.0 0.0 0.12017650924795793 0.0 19 0.0 0.0 0.0 0.13144305698995398 0.0 20 0.0 0.0 0.0 0.13519857290395268 0.0 21 0.0 0.0 0.0 0.14270960473195005 0.0 22 0.0 0.0 0.0 0.15209839451694676 0.0 23 0.0 0.0 0.0 0.16524270021594217 0.0 24 0.0 0.0 0.0 0.17275373204393954 0.0 25 0.0 0.0 0.0 0.1765092479579382 0.0 26 0.0 0.0 0.0 0.1802647638719369 0.0 27 0.0 0.0 0.0 0.21406440709792507 0.0 28 0.0 0.0 0.0 0.2328419866679185 0.0 29 0.0 0.0 0.0 0.268519387850906 0.0 30 0.0 0.0 0.0 0.3041967890338935 0.0 31 0.0 0.0 0.0 0.32672988451788565 0.0 32 0.0 0.0 0.0 0.3774293493568679 0.0 33 0.0 0.0 0.0 0.4168622664538541 0.0 34 0.0 0.0 0.0 0.4562951835508403 0.0 35 0.0 0.0 0.0 0.5445498075298094 0.0 36 0.0 0.0 0.0 0.7323256032297437 0.0 37 0.0 0.0 0.0 1.025255844521641 0.0 38 0.0 0.0 0.0 1.4477513848464933 0.0 39 0.0 0.0 0.0 1.994178950333302 0.0 40 0.0 0.0 0.0 2.521828936250117 0.0 41 0.0 0.0 0.0 3.214721622382875 0.0 42 0.0 0.0 0.0 3.785560041310675 0.0 43 0.0 0.0 0.0 4.512252370669421 0.0 44 0.0 0.0 0.0 5.240822457985166 0.0 45 0.0 0.0 0.0 5.952492723687917 0.0 46 0.0 0.0 0.0 6.707351422401652 0.0 47 0.0 0.0 0.0 7.4809877006853815 0.0 48 0.0 0.0 0.0 8.324101023378086 0.0 49 0.0 0.0 0.0 8.97380527649986 0.0 50 0.0 0.0 0.0 9.625387287578631 0.0 51 0.0 0.0 0.0 10.276969298657404 0.0 52 0.0 0.0 0.0 11.050605576941132 0.0 53 0.0 0.0 0.0 11.7172096516759 0.0 54 0.0 0.0 0.0 12.323725471786688 0.0 55 0.0 0.0 0.0 12.952774387381467 0.0 56 0.0 0.0 0.0 13.551779175664256 0.0 57 0.0 0.0 0.0 14.11135104685006 0.0 58 0.0 0.0 0.0 14.68594498169186 0.0 59 0.0 0.0 0.0 15.217350483522674 0.0 60 0.0 0.0 0.0 15.837010609332458 0.0 61 0.0 0.0 0.0 16.449159703314244 0.0 62 0.0 0.0 0.0 17.00309830062905 0.0 63 0.0 0.0 0.0 17.57205896159985 0.0 64 0.0 0.0 0.0 18.146652896441648 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATTTTG 15 0.002214299 69.99155 65 GTATCAT 15 0.002214299 69.99155 57 ACTGGTG 15 0.002214299 69.99155 8 GTCATGA 20 0.0069191693 52.49366 9 GATACTA 20 0.0069191693 52.49366 47 GGCAGAG 20 0.0069191693 52.49366 21 GAACTGC 20 0.0069191693 52.49366 19 TGTGGCA 20 0.0069191693 52.49366 25 TCTAGGG 20 0.0069191693 52.49366 3 GTAATCA 20 0.0069191693 52.49366 24 GGCTTAT 20 0.0069191693 52.49366 12 ATTTTGC 20 0.0069191693 52.49366 66 CTGTTCT 20 0.0069191693 52.49366 70 GGAAATC 20 0.0069191693 52.49366 27 TAAGGTG 20 0.0069191693 52.49366 49 CGGTAGA 20 0.0069191693 52.49366 14 GCTAATG 20 0.0069191693 52.49366 11 ATTTCCT 20 0.0069191693 52.49366 36 AACAATA 50 0.007153962 27.99662 10 ATGAATG 50 0.007153962 27.99662 15 >>END_MODULE