FastQCFastQC Report
Wed 25 May 2016
SRR1780640_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780640_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53255
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT10151.905924326354333No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT4350.8168247112947141TruSeq Adapter, Index 2 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC1280.24035301849591587No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1060.19904234344193034No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG880.16524270021594217No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCCA150.002213238770.0000112
GTTTTGG150.002213238770.0000123
GACATTG150.002213238770.0000161
AAGATAC150.002213238770.0000126
ATACTGG207.859497E-570.06
AGTAAAG207.859497E-570.022
GGTATAC252.370317E-456.0000043
ATCCTGA200.006915866452.51
CTATGGC200.006915866452.53
GATACCT200.006915866452.528
ACAAATG200.006915866452.524
CAGTTCG200.006915866452.548
TTGTAAT200.006915866452.565
TAACGTT200.006915866452.546
TGACGAT200.006915866452.540
GTTCGAG200.006915866452.550
GGGGTAT200.006915866452.51
AGTTCGA200.006915866452.549
GGCATGG200.006915866452.58
ATAACGT200.006915866452.545
Produced by FastQC (version 0.11.5)