##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780640_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 53255 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.83214721622383 34.0 34.0 34.0 31.0 34.0 2 33.30280724814571 34.0 34.0 34.0 32.0 34.0 3 33.51709698619848 34.0 34.0 34.0 34.0 34.0 4 33.451657121397055 34.0 34.0 34.0 34.0 34.0 5 33.516026664162986 34.0 34.0 34.0 34.0 34.0 6 37.24390198103465 38.0 37.0 38.0 36.0 38.0 7 36.83907614308516 38.0 37.0 38.0 35.0 38.0 8 36.88754107595531 38.0 37.0 38.0 35.0 38.0 9 36.751197070697586 38.0 37.0 38.0 34.0 38.0 10-11 36.444596751478734 38.0 37.0 38.0 34.0 38.0 12-13 36.34319782180077 38.0 37.0 38.0 34.0 38.0 14-15 36.587757018120364 38.0 37.0 38.0 34.0 38.0 16-17 36.71578255562858 38.0 37.0 38.0 34.5 38.0 18-19 36.71029950239414 38.0 37.0 38.0 34.0 38.0 20-21 36.467571120082624 38.0 37.0 38.0 34.0 38.0 22-23 36.53974274715989 38.0 37.0 38.0 34.0 38.0 24-25 35.880386818139144 38.0 36.5 38.0 32.5 38.0 26-27 36.05960942634494 38.0 37.0 38.0 33.0 38.0 28-29 35.95951553844709 38.0 37.0 38.0 32.5 38.0 30-31 35.71377335461459 38.0 36.5 38.0 31.5 38.0 32-33 36.168538165430476 38.0 37.0 38.0 34.0 38.0 34-35 36.21565111257159 38.0 37.0 38.0 33.5 38.0 36-37 36.325565674584546 38.0 37.0 38.0 34.0 38.0 38-39 35.97974838043376 38.0 36.5 38.0 32.5 38.0 40-41 35.529715519669516 38.0 36.0 38.0 31.0 38.0 42-43 35.95446436954276 38.0 36.5 38.0 32.5 38.0 44-45 36.11882452351892 38.0 37.0 38.0 34.0 38.0 46-47 36.400722936813445 38.0 37.0 38.0 34.0 38.0 48-49 36.221190498544736 38.0 37.0 38.0 34.0 38.0 50-51 36.35321566050136 38.0 37.0 38.0 34.0 38.0 52-53 36.264350765186364 38.0 37.0 38.0 34.0 38.0 54-55 36.41711576377805 38.0 37.0 38.0 34.0 38.0 56-57 36.507839639470475 38.0 37.0 38.0 34.0 38.0 58-59 36.52118110975495 38.0 37.0 38.0 34.0 38.0 60-61 36.241789503333024 38.0 37.0 38.0 33.5 38.0 62-63 36.48791662754671 38.0 37.0 38.0 34.0 38.0 64-65 36.50585860482584 38.0 37.0 38.0 34.0 38.0 66-67 35.89760585860483 38.0 37.0 38.0 32.0 38.0 68-69 35.31344474697212 38.0 36.0 38.0 29.0 38.0 70-71 35.23447563609051 38.0 36.0 38.0 29.0 38.0 72-73 34.82373486057647 38.0 36.0 38.0 26.0 38.0 74-75 35.41148248990705 38.0 36.5 38.0 31.0 38.0 76 34.87561731292836 38.0 36.0 38.0 27.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6651776395753615 1101 2 0.46935887007489185 1101 3 0.16262403262793157 1101 4 0.4626624453175694 1101 5 0.2598992822056161 1101 6 -0.9871373758670714 1101 7 0.18577520762379862 1101 8 0.7600651554786779 1101 9 0.6622625083341163 1101 10-11 -0.01368031563468719 1101 12-13 0.7024162371949103 1101 14-15 0.7055484592875487 1101 16-17 0.18479523210760362 1101 18-19 -0.7617588656742029 1101 20-21 -0.435627973672716 1101 22-23 0.5977922125802095 1101 24-25 -2.0816761113350566 1101 26-27 -0.6382064367643778 1101 28-29 0.1416236990983819 1101 30-31 0.5928444968280218 1101 32-33 0.09063371309661505 1101 34-35 -0.06133034502899193 1101 36-37 0.2721288701839555 1101 38-39 0.26612528988214024 1101 40-41 1.2452449934092868 1101 42-43 0.5628258163201281 1101 44-45 0.1538642902727574 1101 46-47 0.5326011291309101 1101 48-49 -1.8275458272429788 1101 50-51 -1.6592023967288796 1101 52-53 -2.515962863455286 1101 54-55 -1.105171968129703 1101 56-57 -0.8430467306347609 1101 58-59 -1.399132552181257 1101 60-61 -1.3216581077643639 1101 62-63 -0.5317146752035526 1101 64-65 -0.7279973863251996 1101 66-67 -2.144595654884938 1101 68-69 -1.7007753526942153 1101 70-71 -0.5937187313317835 1101 72-73 -1.512175800583762 1101 74-75 -0.09259797617502841 1101 76 -1.2376659545725275 1102 1 -1.3371633447800129 1102 2 0.13414132982137517 1102 3 0.27145125656306845 1102 4 0.3534926383060366 1102 5 0.1519285159614654 1102 6 0.005725476708015265 1102 7 -0.5589433032817581 1102 8 0.43723770315482113 1102 9 0.10145401227381967 1102 10-11 0.23463531757303002 1102 12-13 0.09824243338081828 1102 14-15 -0.38218135685954024 1102 16-17 -0.06914447327095274 1102 18-19 -0.6716024194026531 1102 20-21 0.06077490402513064 1102 22-23 0.29925961009077184 1102 24-25 -4.82944932133848 1102 26-27 -1.31968982148517 1102 28-29 -0.4170151041447383 1102 30-31 1.5733172614734485 1102 32-33 0.22055834481979986 1102 34-35 0.33352519910712886 1102 36-37 -3.0714534068515533 1102 38-39 -0.5733174416469211 1102 40-41 0.6333059731968831 1102 42-43 0.24590792206410583 1102 44-45 0.7484457679477643 1102 46-47 0.4453851875984185 1102 48-49 -3.866400457824227 1102 50-51 -0.9630450369024501 1102 52-53 -0.7304769664585891 1102 54-55 -0.14068641371608948 1102 56-57 0.6362840904226772 1102 58-59 -0.008873330986787664 1102 60-61 -6.231130529826721 1102 62-63 -2.9179770938400793 1102 64-65 -0.2678512175459389 1102 66-67 0.547507805204134 1102 68-69 -1.3577640474526973 1102 70-71 -2.212880544738198 1102 72-73 -6.182268298014392 1102 74-75 -2.5606235557113948 1102 76 -1.4245450227465568 1103 1 0.865199089596814 1103 2 0.39342579414181245 1103 3 0.10170597170986895 1103 4 0.3707491034042292 1103 5 0.3684363907427226 1103 6 0.3502714615417659 1103 7 0.8623088841574784 1103 8 0.6450930405065591 1103 9 0.321850667922277 1103 10-11 0.09356979161541545 1103 12-13 -0.28514275036407355 1103 14-15 -0.19948157074248485 1103 16-17 0.34953139684376566 1103 18-19 0.8378764839611463 1103 20-21 0.2941017560570103 1103 22-23 0.5266317664197615 1103 24-25 -2.943001722660668 1103 26-27 -0.56179073534868 1103 28-29 -0.2826577251830358 1103 30-31 0.6517248057083336 1103 32-33 1.016470263933165 1103 34-35 0.21853880984016172 1103 36-37 -0.5754151023600116 1103 38-39 0.12734365110050305 1103 40-41 0.3363003344646245 1103 42-43 -0.27560189710758465 1103 44-45 0.970252106660574 1103 46-47 0.9007371222669036 1103 48-49 -1.407232656929807 1103 50-51 -0.23438472191120496 1103 52-53 0.2275485050560846 1103 54-55 0.07045008249234996 1103 56-57 0.3899541273660958 1103 58-59 -0.10499913304784059 1103 60-61 -3.76535180145806 1103 62-63 -0.8896619081507922 1103 64-65 0.08158004825223486 1103 66-67 -0.13188651717580058 1103 68-69 0.4280856511667892 1103 70-71 0.8556592180461706 1103 72-73 -3.8963456847536513 1103 74-75 -1.4409463245233667 1103 76 -2.4078718747784436 1104 1 -0.26566136269221374 1104 2 0.46595750953067494 1104 3 0.017499316074641058 1104 4 -0.3173829061563538 1104 5 -0.14939776994857823 1104 6 0.19086715928032305 1104 7 0.18690899447187093 1104 8 0.36323975865327895 1104 9 0.8391332566334384 1104 10-11 -0.9739977759521494 1104 12-13 0.3821214526144132 1104 14-15 0.7117842869519464 1104 16-17 0.4552033953729051 1104 18-19 0.6543409075684252 1104 20-21 0.009950257619799174 1104 22-23 0.3364543440994865 1104 24-25 0.05267763016154703 1104 26-27 0.8226779169771135 1104 28-29 1.0078368510258215 1104 30-31 0.6948853131545505 1104 32-33 0.48689221649797076 1104 34-35 0.677331786490285 1104 36-37 1.2352807976216056 1104 38-39 1.1210005733288568 1104 40-41 1.716333428783436 1104 42-43 0.6370888548688782 1104 44-45 0.9095332245131189 1104 46-47 0.79734035815585 1104 48-49 -0.14330546443118664 1104 50-51 0.5250379660829196 1104 52-53 0.5611346422136521 1104 54-55 -0.011067659739673275 1104 56-57 0.14448161403644377 1104 58-59 -0.9932368605712725 1104 60-61 0.3092942731880157 1104 62-63 0.1451560730957624 1104 64-65 0.4023881012031367 1104 66-67 -0.11568409025909432 1104 68-69 1.7533062799588421 1104 70-71 1.672721177965272 1104 72-73 1.5864636551985427 1104 74-75 0.7804178968408451 1104 76 0.03104152842067265 1105 1 0.6928805446367576 1105 2 0.37906237411801413 1105 3 0.16561894128321342 1105 4 -0.05246184339162596 1105 5 0.06867436456560227 1105 6 0.02169760466602355 1105 7 0.5108725421550986 1105 8 -0.6080091182559713 1105 9 0.6020648443628716 1105 10-11 0.4851217209032015 1105 12-13 -0.2975088900887073 1105 14-15 0.221795838742473 1105 16-17 0.07675390236816071 1105 18-19 0.5691785407348959 1105 20-21 0.25619592569835703 1105 22-23 -0.1839538191658292 1105 24-25 0.4657183181348472 1105 26-27 0.4873551086084902 1105 28-29 0.9706144148815525 1105 30-31 1.3480107142583861 1105 32-33 0.7512356897363262 1105 34-35 -0.7491859904318048 1105 36-37 0.19882848084584026 1105 38-39 0.02518488856438239 1105 40-41 0.015460626832464186 1105 42-43 0.33558712210030706 1105 44-45 0.4336636313928537 1105 46-47 0.839055157283056 1105 48-49 -0.2108192624032057 1105 50-51 -0.011029291008597397 1105 52-53 0.15265049383732077 1105 54-55 0.7956965553803386 1105 56-57 0.5058601277060575 1105 58-59 -0.1504053883597507 1105 60-61 0.6264550997828024 1105 62-63 -0.30035798158271376 1105 64-65 0.27499752233008223 1105 66-67 -0.07024704751746924 1105 68-69 -0.7472713095675303 1105 70-71 0.920507084780823 1105 72-73 0.39483228566960804 1105 74-75 -0.22048057575573665 1105 76 0.291983641114804 1106 1 -1.1809231195688774 1106 2 -0.3339537593247002 1106 3 0.1395046399433184 1106 4 0.14209653779514042 1106 5 -0.2780579296646408 1106 6 0.3116894012205762 1106 7 -0.9481102306094655 1106 8 -0.002184671988544551 1106 9 -1.088255090009568 1106 10-11 0.36658193419278007 1106 12-13 0.9023817306386661 1106 14-15 -0.20991047791051898 1106 16-17 0.254153410161436 1106 18-19 0.21253374992275553 1106 20-21 -0.30519319106402065 1106 22-23 -0.060765413368720544 1106 24-25 -0.34772165998929694 1106 26-27 0.3343608510202998 1106 28-29 0.5182627605200523 1106 30-31 0.7346664429977992 1106 32-33 -0.3638176326590852 1106 34-35 0.4752466045914403 1106 36-37 0.6906933974441429 1106 38-39 -0.42573116284387424 1106 40-41 0.39897170148382344 1106 42-43 -0.14404098837276536 1106 44-45 0.6350582171188606 1106 46-47 0.4515107219535395 1106 48-49 0.4730062776569568 1106 50-51 -0.11589666864053783 1106 52-53 0.6142993863721813 1106 54-55 -0.3667316644719989 1106 56-57 0.09035561581106322 1106 58-59 0.0891352186952048 1106 60-61 0.22906652991679266 1106 62-63 -0.026021093423018726 1106 64-65 -0.7764790978503981 1106 66-67 -0.3288606652369026 1106 68-69 0.5336966969952215 1106 70-71 -0.5169416436851293 1106 72-73 0.1379277190849706 1106 74-75 -0.7967042563682583 1106 76 -0.2542291015704592 1107 1 0.5093288881852089 1107 2 -0.14134783298850095 1107 3 0.0851798914895241 1107 4 0.27137743874593667 1107 5 0.08241470026243292 1107 6 0.23493319333725537 1107 7 0.7257105023361632 1107 8 0.2686082754867627 1107 9 0.13481331209511183 1107 10-11 0.5291869374236526 1107 12-13 0.07924871116114218 1107 14-15 0.27118894053312204 1107 16-17 0.7488686321682678 1107 18-19 0.5500902901239968 1107 20-21 0.8223338097667678 1107 22-23 0.2661523379466999 1107 24-25 0.5902225844489877 1107 26-27 0.43087151288682435 1107 28-29 0.6261045402671215 1107 30-31 0.810314924489532 1107 32-33 1.0300483324475351 1107 34-35 0.2791559556483918 1107 36-37 1.032542377412753 1107 38-39 0.572514937036118 1107 40-41 0.9809896097271533 1107 42-43 0.9983480082117495 1107 44-45 0.4978386103617325 1107 46-47 0.8516538032791203 1107 48-49 0.8145783260276716 1107 50-51 0.7992699518708619 1107 52-53 0.3687302919385118 1107 54-55 0.6682078721291234 1107 56-57 0.5795091282268245 1107 58-59 0.9054372587579849 1107 60-61 1.083331276524376 1107 62-63 0.6768082244021443 1107 64-65 0.8017771561881943 1107 66-67 0.10737583434769249 1107 68-69 1.0983602251037823 1107 70-71 -0.0295189537562095 1107 72-73 0.8886431257128677 1107 74-75 -0.9432703045543605 1107 76 0.8043419313844069 1108 1 -0.32215548351935297 1108 2 0.5060980690657431 1108 3 0.10260090637963515 1108 4 0.19502868099506543 1108 5 -0.033120795582675555 1108 6 -0.2570312053635462 1108 7 0.08649001893464003 1108 8 -0.24697783831928177 1108 9 -0.1557759653334969 1108 10-11 0.49951215782268577 1108 12-13 0.8488737164338502 1108 14-15 0.30139099056054164 1108 16-17 -0.2272987445559096 1108 18-19 -0.013519614123701729 1108 20-21 1.0246369269895368 1108 22-23 0.2244347018054782 1108 24-25 0.808027452209565 1108 26-27 -0.15484156763791646 1108 28-29 -0.7204597656340894 1108 30-31 -0.06704197431673009 1108 32-33 0.5896087998200414 1108 34-35 0.6203421031997536 1108 36-37 1.113214752660575 1108 38-39 1.0630873418110838 1108 40-41 0.7959387808580374 1108 42-43 0.015811204735982187 1108 44-45 0.7800337426011552 1108 46-47 0.7921144267236713 1108 48-49 1.033880641799385 1108 50-51 0.5841225270596269 1108 52-53 0.13740621044359358 1108 54-55 0.013823091262715081 1108 56-57 0.06381756033548669 1108 58-59 0.11826469369280801 1108 60-61 0.917304364641808 1108 62-63 0.8033531267516594 1108 64-65 0.6810744101638093 1108 66-67 0.648608381696576 1108 68-69 1.1074081856217717 1108 70-71 -0.03721710198247763 1108 72-73 -0.27329216766040076 1108 74-75 -1.0212219644016614 1108 76 0.20430818607711387 1109 1 -1.0755296137901205 1109 2 0.11733958134674083 1109 3 0.11299574249331101 1109 4 -0.2523265038485931 1109 5 -0.003612873711603015 1109 6 -0.1793214224814932 1109 7 -0.15770480041317114 1109 8 -0.15665174225087952 1109 9 0.15472784890072688 1109 10-11 0.19055918607316613 1109 12-13 0.25993080420315096 1109 14-15 0.5026651962776967 1109 16-17 0.353722296604289 1109 18-19 0.5391252010326397 1109 20-21 0.8903213934158956 1109 22-23 0.13144702471603154 1109 24-25 -0.532550131829332 1109 26-27 -0.21159401964563074 1109 28-29 -1.2701705544680237 1109 30-31 0.37042135035424195 1109 32-33 0.225202730577017 1109 34-35 -0.3790920488666458 1109 36-37 0.815578739583195 1109 38-39 0.7907887417607853 1109 40-41 -0.18995693546352754 1109 42-43 0.701262625643011 1109 44-45 0.3895986684121979 1109 46-47 0.5051247377304691 1109 48-49 0.16572321032985116 1109 50-51 -0.053064160843803165 1109 52-53 -0.467841505207339 1109 54-55 -0.2597329486400426 1109 56-57 0.09150219442049234 1109 58-59 -0.8093696427980888 1109 60-61 0.4262321875942803 1109 62-63 -0.04271527797631336 1109 64-65 0.6822899353545182 1109 66-67 -0.03579517266673804 1109 68-69 -0.7495999458226095 1109 70-71 -0.24611977613788838 1109 72-73 -0.6952680248152348 1109 74-75 -0.6776893967094821 1109 76 0.1984401334322925 1110 1 -0.8847089817398341 1110 2 -0.8129540550951759 1110 3 -0.39066394923148096 1110 4 -0.1541176000339064 1110 5 0.0070648150854353275 1110 6 -0.3737586910598125 1110 7 0.5134396067167728 1110 8 0.4244642484491976 1110 9 0.6962761137762925 1110 10-11 -0.28692294602017654 1110 12-13 0.36511464989332865 1110 14-15 0.5893353073601091 1110 16-17 0.5164278851688238 1110 18-19 -0.36606725569688336 1110 20-21 0.5405625025177585 1110 22-23 -0.6363347715467782 1110 24-25 -0.8929006011309752 1110 26-27 0.6390044475893646 1110 28-29 0.443211000685686 1110 30-31 -0.022801761675381726 1110 32-33 0.19777657023946915 1110 34-35 0.24876035791885442 1110 36-37 1.0414032466011989 1110 38-39 0.9101162195873584 1110 40-41 -0.7020339181553439 1110 42-43 -0.10100638322636257 1110 44-45 0.6476284626083597 1110 46-47 0.5014219908089075 1110 48-49 0.9519326308069083 1110 50-51 -0.9273429862980365 1110 52-53 0.3060326013973267 1110 54-55 -0.6607275236852459 1110 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-2.122524998328906 2116 36-37 0.3199567732750168 2116 38-39 -3.722178803685104 2116 40-41 -1.073677312355123 2116 42-43 -1.505052212084216 2116 44-45 -0.5138466537539799 2116 46-47 -0.4826453203260712 2116 48-49 -0.20077055573086255 2116 50-51 -1.3770386540388202 2116 52-53 -0.4186362134970594 2116 54-55 -1.2396748921063008 2116 56-57 -0.224834533475196 2116 58-59 -2.425030630710914 2116 60-61 -1.6480992105212593 2116 62-63 0.24021514935784438 2116 64-65 -1.7087826828999795 2116 66-67 -1.239207183706995 2116 68-69 -1.2176926459272934 2116 70-71 0.943039831742567 2116 72-73 0.14571893625834065 2116 74-75 0.018513134936085862 2116 76 -1.2740068067028503 2117 1 0.32863593069322405 2117 2 0.6106962560931279 2117 3 0.3878857685279655 2117 4 0.15736018256849604 2117 5 -0.027672484089556804 2117 6 0.016746447165687073 2117 7 0.6850997601398419 2117 8 0.3470290190382883 2117 9 -0.5272005109161384 2117 10-11 -0.3215155064485984 2117 12-13 -0.41907987898205334 2117 14-15 0.21677777370835116 2117 16-17 -0.9608481316208639 2117 18-19 -1.0263654175148034 2117 20-21 0.08564862207196455 2117 22-23 0.553634833316444 2117 24-25 -1.0064481478092162 2117 26-27 -1.7098883009994381 2117 28-29 -0.6413887966800687 2117 30-31 0.2113855302626746 2117 32-33 -1.27030853614351 2117 34-35 0.43909180964848105 2117 36-37 -1.4392017613594348 2117 38-39 0.2583576438166233 2117 40-41 0.38278298733025906 2117 42-43 0.45247458362847226 2117 44-45 -0.27428498362119313 2117 46-47 -0.3183782697208315 2117 48-49 -0.06977818117746182 2117 50-51 -0.004271630095978196 2117 52-53 0.4740742861563305 2117 54-55 -0.04218649025060017 2117 56-57 0.3290235159035717 2117 58-59 -0.49504609490330864 2117 60-61 0.17302274329945533 2117 62-63 -0.06464274004651571 2117 64-65 -0.3963526717017629 2117 66-67 0.3125978608192099 2117 68-69 0.5532773322095323 2117 70-71 -0.0893283226860575 2117 72-73 0.981745172060613 2117 74-75 1.0717046242977872 2117 76 0.4477443797739795 2118 1 -0.07437076194486991 2118 2 0.13971433620500306 2118 3 -0.843859311883584 2118 4 0.24446436796456084 2118 5 0.2154101250403997 2118 6 0.43763347143899267 2118 7 0.9770785834342206 2118 8 0.7394973039249066 2118 9 -1.141225083885871 2118 10-11 -0.769772423839477 2118 12-13 -0.538473545948392 2118 14-15 0.2880993200074258 2118 16-17 -2.571576330601985 2118 18-19 -0.1436613919429348 2118 20-21 -0.4532336074755321 2118 22-23 0.6763748193318335 2118 24-25 0.917485525150525 2118 26-27 -1.7547389652687428 2118 28-29 0.01816069887481575 2118 30-31 -0.7316021832524555 2118 32-33 -0.11443000253049007 2118 34-35 0.2618867352303411 2118 36-37 -2.45545887853055 2118 38-39 -0.3381112404107256 2118 40-41 -0.8771560796960145 2118 42-43 0.4144913651687716 2118 44-45 -2.1780158796355806 2118 46-47 0.14231496771798646 2118 48-49 0.41166722887148666 2118 50-51 -0.7779705612716867 2118 52-53 -1.0244023895004446 2118 54-55 -0.9957375840474256 2118 56-57 0.24201922062837156 2118 58-59 -0.78757431386245 2118 60-61 -0.9319867462620195 2118 62-63 -0.8881868415348819 2118 64-65 0.038993447567037265 2118 66-67 0.5180468669125133 2118 68-69 -0.7279383305614218 2118 70-71 -0.4496319886534934 2118 72-73 1.1201179619916815 2118 74-75 0.41287933211918215 2118 76 0.5225620803033948 2119 1 0.8969611666344761 2119 2 -0.8671615648707416 2119 3 -0.0710967522739594 2119 4 -0.5053415541824933 2119 5 -0.431201830155338 2119 6 -0.540130751695088 2119 7 -1.5704427651296058 2119 8 0.3452420083720682 2119 9 -0.7029418411221968 2119 10-11 -0.2732103743773493 2119 12-13 -0.2942404307216009 2119 14-15 -0.30910595891018033 2119 16-17 -0.148819316073876 2119 18-19 0.22311802471449482 2119 20-21 -1.6482263988695678 2119 22-23 -0.4739800658849873 2119 24-25 0.6645743573563578 2119 26-27 0.22052997118463225 2119 28-29 -0.5522895617440113 2119 30-31 0.46854753394838866 2119 32-33 0.3368841817880224 2119 34-35 0.31927832888677443 2119 36-37 -0.33038956501163597 2119 38-39 -1.72762549127021 2119 40-41 -0.9380133492978757 2119 42-43 0.05492055447786015 2119 44-45 -0.33241867435666705 2119 46-47 -0.21883619120404774 2119 48-49 -0.5273645998963943 2119 50-51 0.4848108516150873 2119 52-53 -0.1993774366336325 2119 54-55 0.20710149768707709 2119 56-57 -1.3877911375838963 2119 58-59 0.14704917510283622 2119 60-61 0.41128152478156466 2119 62-63 -0.39766893092158995 2119 64-65 0.0015364219487565833 2119 66-67 0.3524984583509081 2119 68-69 1.2348264470808132 2119 70-71 -0.05166148848714158 2119 72-73 1.2291065391237694 2119 74-75 -0.221644345378877 2119 76 0.18209407542372702 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 1.0 21 0.0 22 3.0 23 12.0 24 21.0 25 42.0 26 89.0 27 146.0 28 260.0 29 446.0 30 743.0 31 1104.0 32 1758.0 33 2865.0 34 4520.0 35 6998.0 36 12172.0 37 22074.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.33564923481363 23.751760398084688 12.113416580602761 28.799173786498923 2 17.051920007511033 19.814101962257066 40.15585391043094 22.97812411980096 3 16.843488874284105 25.498075298094076 31.386724251244015 26.271711576377804 4 12.522767815228617 20.5708384189278 39.45732795042719 27.44906581541639 5 11.927518542859826 37.72791287203079 37.78236785278378 12.562200732325604 6 26.22852314336682 41.30691953807154 18.11660876912966 14.347948549431976 7 25.779738991643974 30.241291897474415 24.161111632710544 19.817857478171064 8 21.751948173880386 37.89127781428974 21.19425406065158 19.162519951178293 9 22.45610740775514 15.305605107501643 22.983757393671954 39.25453009107126 10-11 24.49159703314243 26.54304760116421 28.141019622570653 20.824335743122713 12-13 23.537695990986762 22.97061308797296 27.45000469439489 26.041686226645382 14-15 21.85991925640785 24.475636090507933 23.59778424561074 30.066660407473478 16-17 22.765937470660035 28.248990705098116 25.436109285513098 23.54896253872876 18-19 22.835414515069008 26.78527837761713 26.176884799549338 24.20242230776453 20-21 21.31067505398554 27.030325791005538 25.577880011266547 26.08111914374237 22-23 21.82987512909586 28.191719087409634 26.844427753262607 23.1339780302319 24-25 22.249554032485214 28.873345225800396 26.39564360153976 22.481457140174633 26-27 22.531217726035113 29.19725847338278 26.68294056896066 21.588583231621445 28-29 23.62407285700873 26.094263449441367 27.241573561167964 23.040090132381934 30-31 23.02694582668294 27.74387381466529 26.538353206271715 22.69082715238006 32-33 21.514411792319972 28.556004131067503 26.873533001596094 23.05605107501643 34-35 22.835414515069008 27.161768847995493 26.944887803962068 23.057928832973428 36-37 22.081494695333774 27.192751854285984 27.874377992676745 22.8513754577035 38-39 21.25622007323256 27.10449723030701 28.39357806778706 23.245704628673362 40-41 20.962350952962165 25.871749131536948 29.433855975964697 23.732043939536194 42-43 22.636372171627077 25.353487935405127 29.394423058867712 22.615716834100084 44-45 20.40184020279786 26.871655243639097 29.18974744155478 23.53675711200826 46-47 22.34625856727068 25.52060839357807 28.446155290583043 23.68697774856821 48-49 21.415829499577505 25.073701999812226 28.949394423058866 24.561074077551403 50-51 19.928645197634026 25.647357055675524 29.669514599568114 24.754483147122336 52-53 19.104309454511316 25.448314712233593 32.103088911839265 23.34428692141583 54-55 18.504365787250023 25.984414608956907 30.752042061778234 24.759177542014836 56-57 20.359590648765373 25.79851657121397 29.03764904703784 24.80424373298282 58-59 18.94094451225237 26.97493193127406 30.2732137827434 23.810909773730167 60-61 20.25725284010891 28.57947610553 27.361750070415923 23.80152098394517 62-63 18.235846399399115 30.237536381560414 26.922354708478075 24.60426251056239 64-65 19.092104027790818 30.417801145432357 25.375082151910615 25.11501267486621 66-67 19.00760491972585 30.30983006290489 25.271805464275655 25.410759553093605 68-69 19.014177072575343 30.192470190592434 25.274622101211154 25.51873063562107 70-71 19.62163177166463 29.843207210590556 24.963853159327762 25.571307858417054 72-73 19.642287109191624 28.915594779832883 24.42399774669045 27.018120364285043 74-75 20.58961599849779 28.928739085531873 23.978969110881607 26.502675805088728 76 20.476950521077832 28.414233405314054 24.26251056238851 26.846305511219605 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.5 15 1.5 16 0.0 17 0.0 18 3.0 19 5.5 20 5.0 21 5.0 22 11.5 23 23.0 24 31.5 25 35.0 26 87.0 27 158.5 28 230.5 29 283.0 30 321.0 31 428.0 32 635.5 33 774.0 34 921.0 35 1195.5 36 1391.5 37 1460.0 38 1732.5 39 2326.0 40 2949.0 41 3572.5 42 3894.0 43 4029.0 44 4320.5 45 4499.0 46 4521.0 47 4404.5 48 4057.0 49 3475.5 50 3125.0 51 2969.0 52 2482.5 53 1977.5 54 1803.0 55 1636.5 56 1237.5 57 836.0 58 667.0 59 526.0 60 312.0 61 194.0 62 149.0 63 117.0 64 63.0 65 28.5 66 15.5 67 15.0 68 11.5 69 10.0 70 7.5 71 3.0 72 3.5 73 3.5 74 2.5 75 2.0 76 1.5 77 1.0 78 1.0 79 1.0 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 53255.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.821612994085065 #Duplication Level Percentage of deduplicated Percentage of total 1 56.9576083368699 22.68143836259506 2 15.88626396944405 12.652333114261571 3 9.199792521337294 10.990517322317153 4 5.474607440939312 8.720307952304948 5 3.343235724053378 6.65665195756267 6 2.277549865610412 5.441742559384095 7 1.5372282736832177 4.2850436578725 8 1.2590182486914698 4.0108909961505965 9 0.9100768614136843 3.261665571307858 >10 3.1310416371952656 17.97202140644071 >50 0.004715424152402508 0.16524270021594217 >100 0.014146272457207527 1.2562200732325604 >500 0.0 0.0 >1k 0.004715424152402508 1.905924326354333 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT 1015 1.905924326354333 No Hit ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT 435 0.8168247112947141 TruSeq Adapter, Index 2 (95% over 21bp) CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC 128 0.24035301849591587 No Hit ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 106 0.19904234344193034 No Hit TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG 88 0.16524270021594217 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.005633273870998028 0.0 2 0.0 0.0 0.0 0.015022063655994742 0.0 3 0.0 0.0 0.0 0.02065533752699277 0.0 4 0.0 0.0 0.0 0.02065533752699277 0.0 5 0.0 0.0 0.0 0.02065533752699277 0.0 6 0.0 0.0 0.0 0.02065533752699277 0.0 7 0.0 0.0 0.0 0.02065533752699277 0.0 8 0.0 0.0 0.0 0.02065533752699277 0.0 9 0.0 0.0 0.0 0.02816636935499014 0.0 10 0.0 0.0 0.0 0.0525772227959816 0.0 11 0.0 0.0 0.0 0.06572152849497699 0.0 12 0.0 0.0 0.0 0.07135480236597502 0.0 13 0.0 0.0 0.0 0.07698807623697305 0.0 14 0.0 0.0 0.0 0.08449910806497042 0.0 15 0.0 0.0 0.0 0.09952117172096517 0.0 16 0.0 0.0 0.0 0.1182987512909586 0.0 17 0.0 0.0 0.0 0.12205426720495728 0.0 18 0.0 0.0 0.0 0.12580978311895596 0.0 19 0.0 0.0 0.0 0.12956529903295466 0.0 20 0.0 0.0 0.0 0.13519857290395268 0.0 21 0.0 0.0 0.0 0.14270960473195005 0.0 22 0.0 0.0 0.0 0.1483428786029481 0.0 23 0.0 0.0 0.0 0.16899821612994084 0.0 24 0.0 0.0 0.0 0.17275373204393954 0.0 25 0.0 0.0 0.0 0.17838700591493756 0.0 26 0.0 0.0 0.0 0.18214252182893625 0.0 27 0.0 0.0 0.0 0.21781992301192377 0.0 28 0.0 0.0 0.0 0.24035301849591587 0.0 29 0.0 0.0 0.0 0.27415266172190406 0.0 30 0.0 0.0 0.0 0.31358557881889026 0.0 31 0.0 0.0 0.0 0.34362970613087973 0.0 32 0.0 0.0 0.0 0.394329170969862 0.0 33 0.0 0.0 0.0 0.43188433010984884 0.0 34 0.0 0.0 0.0 0.47507276312083374 0.0 35 0.0 0.0 0.0 0.5670829030138015 0.0 36 0.0 0.0 0.0 0.7492254248427378 0.0 37 0.0 0.0 0.0 1.0703220354896255 0.0 38 0.0 0.0 0.0 1.5435170406534597 0.0 39 0.0 0.0 0.0 2.1218664914092575 0.0 40 0.0 0.0 0.0 2.700215942165055 0.0 41 0.0 0.0 0.0 3.417519481738804 0.0 42 0.0 0.0 0.0 4.018402027978594 0.0 43 0.0 0.0 0.0 4.792038306262323 0.0 44 0.0 0.0 0.0 5.576941132288048 0.0 45 0.0 0.0 0.0 6.350577410571777 0.0 46 0.0 0.0 0.0 7.127969204769506 0.0 47 0.0 0.0 0.0 7.982349075204207 0.0 48 0.0 0.0 0.0 8.885550652520891 0.0 49 0.0 0.0 0.0 9.587832128438643 0.0 50 0.0 0.0 0.0 10.288235846399399 0.0 51 0.0 0.0 0.0 10.988639564360154 0.0 52 0.0 0.0 0.0 11.826119613181861 0.0 53 0.0 0.0 0.0 12.54154539479861 0.0 54 0.0 0.0 0.0 13.185616374049385 0.0 55 0.0 0.0 0.0 13.852220448784152 0.0 56 0.0 0.0 0.0 14.488780396206929 0.0 57 0.0 0.0 0.0 15.099051732231715 0.0 58 0.0 0.0 0.0 15.696178762557507 0.0 59 0.0 0.0 0.0 16.257628391700308 0.0 60 0.0 0.0 0.0 16.920476950521078 0.0 61 0.0 0.0 0.0 17.562670171814855 0.0 62 0.0 0.0 0.0 18.14477513848465 0.0 63 0.0 0.0 0.0 18.753168716552437 0.0 64 0.0 0.0 0.0 19.378462116233216 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCGCCA 15 0.0022132387 70.00001 12 GTTTTGG 15 0.0022132387 70.00001 23 GACATTG 15 0.0022132387 70.00001 61 AAGATAC 15 0.0022132387 70.00001 26 ATACTGG 20 7.859497E-5 70.0 6 AGTAAAG 20 7.859497E-5 70.0 22 GGTATAC 25 2.370317E-4 56.000004 3 ATCCTGA 20 0.0069158664 52.5 1 CTATGGC 20 0.0069158664 52.5 3 GATACCT 20 0.0069158664 52.5 28 ACAAATG 20 0.0069158664 52.5 24 CAGTTCG 20 0.0069158664 52.5 48 TTGTAAT 20 0.0069158664 52.5 65 TAACGTT 20 0.0069158664 52.5 46 TGACGAT 20 0.0069158664 52.5 40 GTTCGAG 20 0.0069158664 52.5 50 GGGGTAT 20 0.0069158664 52.5 1 AGTTCGA 20 0.0069158664 52.5 49 GGCATGG 20 0.0069158664 52.5 8 ATAACGT 20 0.0069158664 52.5 45 >>END_MODULE