FastQCFastQC Report
Wed 25 May 2016
SRR1780639_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780639_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421686
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGTA207.8289985E-444.02483736
TATCGGA254.451262E-543.97780226
TTTCGAC404.132562E-738.4805828
ATTTCGA404.132562E-738.4805827
TATCGCG357.358205E-637.6550255
ATCGCGT357.358205E-637.6550256
GACGACG301.2962914E-436.68300612
TTATCGG301.3017398E-436.6568725
TCGGAGC301.3117776E-436.6090553
TACTTAG250.002336364735.24514
GAAGTCG250.002355513235.18641730
TAGTCCC250.002369266535.1446954
AACGAAT451.0190852E-634.29043619
TCGACTC451.0382137E-634.20901530
TTCGACT451.0382137E-634.20901529
CGTGCAC401.8161623E-533.00293710
TCGCGTG401.8369317E-532.948157
ATCGGAA353.2238575E-431.41271427
CGGTGGT502.3769026E-630.76620143
CGCAATC801.2914825E-1030.2634812
Produced by FastQC (version 0.11.5)