SRR1780639_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1780639_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	421686
Sequences flagged as poor quality	0
Sequence length	50
%GC	46

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT	12714	3.015039626641624	No Hit
ATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTT	4497	1.0664333176818772	RNA PCR Primer, Index 34 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC	2077	0.4925465867968109	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG	1160	0.2750862015812714	No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC	900	0.21342894950271055	No Hit
TATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCT	484	0.11477734617701323	RNA PCR Primer, Index 34 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTTATA	25	0.00234932	35.205315	2
ACTGGTC	25	0.00234932	35.205315	14
CGGAATA	25	0.002352056	35.196964	16
TGGAATC	25	0.002352056	35.196964	36
ACGGTCT	25	0.002352056	35.196964	43
CCGTCTT	585	0.0	34.971344	44
AGCAAGG	40	1.8168745E-5	33.00107	5
CGTTAAA	40	1.8183497E-5	32.997154	17
AACCGAT	55	1.3711724E-7	31.997238	33
CGCACCA	55	1.3711724E-7	31.997238	16
GCCGTCT	650	0.0	31.47421	43
CGTTCGG	35	3.2159625E-4	31.425861	24
TTCGGTA	35	3.2159625E-4	31.425861	26
GCGCTAT	50	2.3582252E-6	30.797344	19
TCCGTAT	65	1.738772E-8	30.466137	13
TGAATTG	65	1.7427737E-8	30.458914	43
ACACCTT	30	0.0057365904	29.337763	11
TTCGGAA	30	0.0057365904	29.337763	14
TTGCACT	30	0.0057365904	29.337763	13
GGCCGTT	45	4.0591523E-5	29.337759	14