FastQCFastQC Report
Wed 25 May 2016
SRR1780638_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780638_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114773
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGCC150.002220557869.993935
ATCCGGC150.002220557869.99399
GTTACCT150.002220557869.993915
AGCCTTA150.002220557869.993965
GCCCAGC200.006938571652.49542239
GTTCTGC200.006938571652.49542224
GGTCGTT200.006938571652.49542211
GAAGATA200.006938571652.49542214
ATTAACG200.006938571652.49542239
TGAAAGT200.006938571652.49542229
GGCCCAG200.006938571652.49542238
CGTTACC200.006938571652.49542214
ACCTAGA200.006938571652.49542218
GCCTGGC200.006938571652.49542230
GTCGTTA200.006938571652.49542212
TACCTAG200.006938571652.49542217
TGCCTGG200.006938571652.49542229
CTCGCTT200.006938571652.49542216
CATCGTT200.006938571652.49542232
GAGTTAC200.006938571652.49542261
Produced by FastQC (version 0.11.5)