##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780638_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 114773 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.86036785655163 34.0 31.0 34.0 12.0 34.0 2 31.348522736183597 34.0 32.0 34.0 23.0 34.0 3 31.640481646380245 34.0 33.0 34.0 23.0 34.0 4 31.892579265158183 34.0 34.0 34.0 23.0 34.0 5 31.650876077126153 34.0 34.0 34.0 23.0 34.0 6 34.717712353950844 38.0 36.0 38.0 26.0 38.0 7 33.84479799255922 37.0 34.0 38.0 23.0 38.0 8 32.75543899697664 37.0 32.0 38.0 12.0 38.0 9 33.58342118791005 37.0 34.0 38.0 21.0 38.0 10-11 34.0560149164002 37.0 34.0 38.0 23.5 38.0 12-13 34.50196910423183 38.0 35.0 38.0 25.0 38.0 14-15 34.06623509013444 37.0 34.0 38.0 23.5 38.0 16-17 33.940922516619764 37.0 34.0 38.0 23.0 38.0 18-19 33.911460012372245 37.0 34.0 38.0 22.5 38.0 20-21 34.017578176051856 37.0 34.0 38.0 23.0 38.0 22-23 34.00149425387504 37.0 34.0 38.0 23.0 38.0 24-25 34.466111367656154 38.0 35.0 38.0 25.0 38.0 26-27 34.548068796668204 38.0 35.5 38.0 25.0 38.0 28-29 34.70406802993735 38.0 36.0 38.0 26.0 38.0 30-31 34.53228111141122 38.0 35.0 38.0 25.0 38.0 32-33 34.25480731530935 38.0 34.0 38.0 24.0 38.0 34-35 34.081269113815964 37.0 34.0 38.0 23.5 38.0 36-37 34.07299190576181 37.0 34.0 38.0 23.5 38.0 38-39 34.67789462678505 38.0 35.5 38.0 26.0 38.0 40-41 34.122694361914384 37.5 34.0 38.0 23.5 38.0 42-43 34.50028752406925 38.0 35.0 38.0 25.5 38.0 44-45 33.49049428001359 37.0 34.0 38.0 22.0 38.0 46-47 34.272202521498954 37.0 34.0 38.0 24.0 38.0 48-49 33.97206660102986 37.0 34.0 38.0 23.5 38.0 50-51 33.93483659048731 37.0 34.0 38.0 23.0 38.0 52-53 33.9463898303608 37.0 34.0 38.0 23.5 38.0 54-55 33.61834664947331 37.0 34.0 38.0 22.5 38.0 56-57 33.78097636203637 37.0 34.0 38.0 23.0 38.0 58-59 33.65048835527519 37.0 33.5 38.0 22.5 38.0 60-61 33.64820123199707 37.0 33.5 38.0 22.5 38.0 62-63 33.73576973678478 37.0 34.0 38.0 23.0 38.0 64-65 33.74612495970307 37.0 34.0 38.0 23.0 38.0 66-67 33.676125918116625 37.0 34.0 38.0 23.0 38.0 68-69 33.238440225488574 37.0 32.5 38.0 21.5 38.0 70-71 33.078393873123474 37.0 32.5 38.0 20.5 38.0 72-73 33.160220609376765 37.0 32.0 38.0 21.5 38.0 74-75 32.76228729753513 37.0 31.5 38.0 20.0 38.0 76 31.830465353349656 36.0 30.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.962027330061961 1101 2 -0.8386771810421116 1101 3 -0.5186385597163152 1101 4 -3.3027330617882704 1101 5 -1.5267718401678287 1101 6 -2.171380445636089 1101 7 -1.7809719747029717 1101 8 -0.4842214913177969 1101 9 -0.7942687486536428 1101 10-11 -0.976742226888156 1101 12-13 -1.6560460377976511 1101 14-15 -0.6580828146154474 1101 16-17 -1.596642266139213 1101 18-19 -0.3766794145846788 1101 20-21 -0.9897737098837638 1101 22-23 -0.50839905078778 1101 24-25 -1.292631237682123 1101 26-27 -1.7381778717157914 1101 28-29 -0.2915145467677931 1101 30-31 -1.4591831676904903 1101 32-33 -0.32144103320826645 1101 34-35 -1.3998957782807153 1101 36-37 0.01677502733650016 1101 38-39 -0.7383635393829806 1101 40-41 0.48510989662332804 1101 42-43 0.08115575969569022 1101 44-45 0.06095050896613685 1101 46-47 0.27990154348351126 1101 48-49 -1.0173852791201838 1101 50-51 -0.7659733330764311 1101 52-53 0.05149900443544908 1101 54-55 -1.3803524886899439 1101 56-57 -0.4571952613246495 1101 58-59 -0.20328748241132644 1101 60-61 -0.6178723096762582 1101 62-63 -1.8942906424015789 1101 64-65 -0.8611355500725963 1101 66-67 -1.4030340292785581 1101 68-69 -0.011293174739222422 1101 70-71 0.49911404228654277 1101 72-73 -0.1883645328819128 1101 74-75 -0.9454903100260736 1101 76 -1.8402942090610495 1102 1 0.13027489895901923 1102 2 -0.3841044100630917 1102 3 0.3043509507731983 1102 4 0.28760238401592275 1102 5 0.6528063940979045 1102 6 0.5799853760422309 1102 7 1.5355708574648617 1102 8 1.9175486135773099 1102 9 1.4179253072904103 1102 10-11 -1.1389198929720763 1102 12-13 -4.167027244091358 1102 14-15 -0.8667803670629937 1102 16-17 -2.3258954348455063 1102 18-19 -0.0885183568923722 1102 20-21 0.23038308644490968 1102 22-23 -0.1100025254381265 1102 24-25 0.8799676478073835 1102 26-27 -5.772514628708798 1102 28-29 0.31283422945598005 1102 30-31 0.6382408926241965 1102 32-33 -6.445198135480993 1102 34-35 -0.18561201516882875 1102 36-37 0.2813210282106269 1102 38-39 0.6652504247778595 1102 40-41 0.5514615137561947 1102 42-43 0.28363882350687675 1102 44-45 -5.554598005019876 1102 46-47 -0.013299396201809088 1102 48-49 0.5310194411595361 1102 50-51 -6.653101829579928 1102 52-53 -1.116456087347764 1102 54-55 1.0724583723240428 1102 56-57 -1.0385436004854895 1102 58-59 1.0880149651411273 1102 60-61 0.6418933153237418 1102 62-63 -4.746467762156826 1102 64-65 -0.33466592594672306 1102 66-67 0.628202312504655 1102 68-69 -7.796804538375586 1102 70-71 0.020734999454369074 1102 72-73 0.5171643132719339 1102 74-75 -6.41988115355754 1102 76 -1.6877046111589529 1103 1 -0.07096830228418227 1103 2 0.25849681587146733 1103 3 0.1549725513947955 1103 4 0.04550189191543197 1103 5 0.8537258450173546 1103 6 -1.108976896253374 1103 7 1.0429091981365346 1103 8 1.4330238790525769 1103 9 0.7848207575191992 1103 10-11 0.9175961218772741 1103 12-13 -0.9283261303902464 1103 14-15 -0.4902587405413712 1103 16-17 -0.8253990176824217 1103 18-19 -0.2712106645846788 1103 20-21 -0.2634764490812955 1103 22-23 -0.17701884245445143 1103 24-25 0.6133052978116993 1103 26-27 -0.41366976986394377 1103 28-29 0.6261166260717133 1103 30-31 0.04243238014901607 1103 32-33 -5.872246395862586 1103 34-35 -1.1416251378486137 1103 36-37 -0.16245433223139827 1103 38-39 0.6563364991972662 1103 40-41 0.8818595108208598 1103 42-43 0.656556029757418 1103 44-45 -4.028266312021515 1103 46-47 0.06681801724894143 1103 48-49 0.4496286097687019 1103 50-51 0.16593253112110062 1103 52-53 0.08798145042310779 1103 54-55 0.4259505591495625 1103 56-57 1.1925925473173251 1103 58-59 0.833363752156572 1103 60-61 1.340967389397818 1103 62-63 0.9319294579070672 1103 64-65 1.2588007925199989 1103 66-67 -0.11944510180941847 1103 68-69 -3.8097113846157633 1103 70-71 0.406159759879138 1103 72-73 0.971936393044011 1103 74-75 -1.327892894902618 1103 76 -0.3751128819005558 1104 1 -1.0169703791273825 1104 2 0.47965177145010074 1104 3 0.2782851162961464 1104 4 0.12606610098431403 1104 5 -0.05809485418652116 1104 6 0.06516063691842788 1104 7 1.1815961717144745 1104 8 1.7487905803332922 1104 9 0.1781806282933971 1104 10-11 -0.07719734760466679 1104 12-13 -0.21774288359203808 1104 14-15 0.583642263266178 1104 16-17 -0.06801468631054775 1104 18-19 0.7798017964745085 1104 20-21 1.21481765499162 1104 22-23 0.6812366092589528 1104 24-25 0.9247740874736436 1104 26-27 1.0171886586580428 1104 28-29 0.017140048247782147 1104 30-31 -0.724643886148435 1104 32-33 -0.7973646605447087 1104 34-35 0.588269866189691 1104 36-37 0.7138581172897673 1104 38-39 0.08465656381016373 1104 40-41 1.3891573070594632 1104 42-43 1.35962514248385 1104 44-45 -3.458057473899906 1104 46-47 0.35746068483864946 1104 48-49 1.445474923372025 1104 50-51 1.6463611684811994 1104 52-53 -0.014937668072342092 1104 54-55 -0.2803780435964853 1104 56-57 1.2647916935040158 1104 58-59 1.0867241998316644 1104 60-61 0.22522664865707043 1104 62-63 0.9928357692027774 1104 64-65 0.5762549288993668 1104 66-67 0.4737243916715954 1104 68-69 -3.567903377231435 1104 70-71 -0.1922739542087868 1104 72-73 1.0798889717909859 1104 74-75 -0.35555529250270723 1104 76 1.3608107364218185 1105 1 -0.6161879405270767 1105 2 -0.027176454297926256 1105 3 -0.3224176294837555 1105 4 -0.21343436992780696 1105 5 0.023191822622870006 1105 6 -0.241692945636089 1105 7 0.6365425601807502 1105 8 0.5597465319380177 1105 9 0.684655669951006 1105 10-11 0.7233849533444001 1105 12-13 -1.5784660959371877 1105 14-15 -0.5454374657782424 1105 16-17 0.6393224867677674 1105 18-19 0.5383823586711358 1105 20-21 -0.5921083755814394 1105 22-23 0.027438521886644196 1105 24-25 0.09767581173647955 1105 26-27 -0.07075417985532795 1105 28-29 -1.1363546630468662 1105 30-31 -0.44678309501607316 1105 32-33 0.24573738248940913 1105 34-35 0.4626133060216091 1105 36-37 0.5493422511737052 1105 38-39 0.1778337716635292 1105 40-41 0.14363097220471843 1105 42-43 0.2430834486491733 1105 44-45 1.6614228926870638 1105 46-47 0.9801649882509551 1105 48-49 0.1377746046007431 1105 50-51 -0.1406099609834115 1105 52-53 -1.3575217077738557 1105 54-55 -1.3859393346201756 1105 56-57 -0.7909525287665105 1105 58-59 -0.39878166845783625 1105 60-61 0.05032354788188087 1105 62-63 -0.0336892615876252 1105 64-65 0.08045238597391347 1105 66-67 0.37217490967493205 1105 68-69 0.9418318252607776 1105 70-71 1.1943811207749135 1105 72-73 0.45734767642041163 1105 74-75 -1.4949543361888615 1105 76 -1.4716532206889568 1106 1 0.3979644462538303 1106 2 0.14425867422104588 1106 3 -0.762059612347894 1106 4 -0.4509649696830067 1106 5 0.22775118614796241 1106 6 0.29120179120601364 1106 7 1.1705082884549256 1106 8 0.8883114034190456 1106 9 1.6744812513463572 1106 10-11 0.08966402311184396 1106 12-13 0.5145954095707737 1106 14-15 -0.10709597251018721 1106 16-17 0.503995069387102 1106 18-19 -0.3485132961636239 1106 20-21 -0.45410347962060627 1106 22-23 0.27089782421222 1106 24-25 0.30039918994945936 1106 26-27 0.14730732565262628 1106 28-29 -0.8976000730835807 1106 30-31 -0.5750343190062779 1106 32-33 0.9185877496864734 1106 34-35 0.26509188619296964 1106 36-37 -0.03040836082139009 1106 38-39 -0.13772620385666556 1106 40-41 0.09222338346543779 1106 42-43 0.8004197399588548 1106 44-45 1.2801116931766572 1106 46-47 0.6253990763782511 1106 48-49 0.23179235245876129 1106 50-51 0.5736648248183087 1106 52-53 -0.013159712669811086 1106 54-55 0.01736543894163134 1106 56-57 0.8252882913069257 1106 58-59 -0.08651116662184677 1106 60-61 0.5645906837447967 1106 62-63 1.0443401141773663 1106 64-65 1.1366029367695134 1106 66-67 0.9492480430898667 1106 68-69 0.7411739305239351 1106 70-71 0.2938303251812826 1106 72-73 0.7927209934338819 1106 74-75 -1.7276654744997586 1106 76 -0.584948814324207 1107 1 0.41733579128306886 1107 2 0.5656768028760162 1107 3 0.46052810695034907 1107 4 0.33741222329944875 1107 5 0.5471956305924017 1107 6 1.068248574831749 1107 7 0.7794264171098959 1107 8 1.514407894647114 1107 9 -0.3660889240922387 1107 10-11 0.14850905235160639 1107 12-13 0.48955885986316616 1107 14-15 -0.06287082631135377 1107 16-17 0.9780447769894423 1107 18-19 1.6344910898012799 1107 20-21 0.8382941811981084 1107 22-23 -0.16386094771760185 1107 24-25 0.19257755252255748 1107 26-27 0.6979286706818755 1107 28-29 0.03661045323220691 1107 30-31 0.5245636342100894 1107 32-33 1.6285658198619117 1107 34-35 1.2406035820994035 1107 36-37 0.4800083058452671 1107 38-39 1.0351539131023983 1107 40-41 1.0776035004245017 1107 42-43 1.1942428393740556 1107 44-45 0.83219502651 1107 46-47 0.9431987839805842 1107 48-49 1.1236052179558342 1107 50-51 1.7527583920697651 1107 52-53 1.5535435037044536 1107 54-55 1.3729940939123964 1107 56-57 1.5213774725934783 1107 58-59 1.6974800614483172 1107 60-61 0.24277050830619373 1107 62-63 -0.5338762600916382 1107 64-65 0.6143076151320841 1107 66-67 1.8500155869495103 1107 68-69 1.6194634042081475 1107 70-71 0.30260225500584426 1107 72-73 0.985337952498206 1107 74-75 0.8015742915821136 1107 76 -0.10724413596163274 1108 1 -0.7407213887039354 1108 2 0.2770153343899864 1108 3 0.1487997118886213 1108 4 -0.025485762405555334 1108 5 -0.29133588337770533 1108 6 0.5546033506602086 1108 7 0.9935264820871552 1108 8 1.5780856074476333 1108 9 -0.04851257581413648 1108 10-11 0.3157442700254265 1108 12-13 0.690501030103583 1108 14-15 0.9835066915573876 1108 16-17 -0.7358928448429154 1108 18-19 -0.030469923843931213 1108 20-21 0.5883754027705521 1108 22-23 -0.1924509412198816 1108 24-25 -0.29255889971916105 1108 26-27 -0.3735463130738168 1108 28-29 0.4316721816272704 1108 30-31 0.7800867011366748 1108 32-33 0.7357782954954359 1108 34-35 -0.04903254525601852 1108 36-37 0.05976788999081606 1108 38-39 1.0436821782096146 1108 40-41 0.6966743256356693 1108 42-43 1.07939553593026 1108 44-45 0.5072275151389718 1108 46-47 0.5421266592242517 1108 48-49 0.16876441223784155 1108 50-51 0.244636234824803 1108 52-53 0.6095863886947086 1108 54-55 0.5061974727298093 1108 56-57 0.8114197078111474 1108 58-59 -1.155833778707617 1108 60-61 1.2005352906323878 1108 62-63 0.6047689640799021 1108 64-65 0.9270106690632076 1108 66-67 -0.04845744748843117 1108 68-69 1.10541207217436 1108 70-71 2.2101721055581507 1108 72-73 0.905578368352657 1108 74-75 1.1396996976899771 1108 76 0.7143932909389505 1109 1 -0.3960833581684682 1109 2 -0.8962076913971408 1109 3 0.3234472391002541 1109 4 -0.14488820232081423 1109 5 0.17297852965465665 1109 6 0.6636324981508963 1109 7 -1.411981590087585 1109 8 0.6588073548360498 1109 9 0.2880167543049339 1109 10-11 0.9502409461887638 1109 12-13 -0.1397276206378848 1109 14-15 -0.09835648325449853 1109 16-17 -1.0647335198374392 1109 18-19 -0.09147693677402913 1109 20-21 0.7927822257671266 1109 22-23 0.8875768182950594 1109 24-25 0.996697072229118 1109 26-27 0.6560760114203035 1109 28-29 0.4005157786759952 1109 30-31 0.30203863334501335 1109 32-33 1.2524887929751642 1109 34-35 0.36710449908614606 1109 36-37 0.5918374348808797 1109 38-39 0.7521250885755961 1109 40-41 0.6369913685168171 1109 42-43 0.686543611027048 1109 44-45 0.13986138174720253 1109 46-47 -0.028495738321218766 1109 48-49 -0.08127211343970941 1109 50-51 1.0712123178111455 1109 52-53 1.356244289198763 1109 54-55 0.676681072700589 1109 56-57 0.5994338067226863 1109 58-59 0.37862819806204584 1109 60-61 0.8682246762704864 1109 62-63 -0.4277480203601556 1109 64-65 0.5160602709333162 1109 66-67 0.5918924685025075 1109 68-69 1.5831040146098871 1109 70-71 0.5790997116652434 1109 72-73 -0.3985161601008471 1109 74-75 1.7542716167490795 1109 76 -0.37732268539241076 1110 1 0.07433084301496251 1110 2 0.7087627228040425 1110 3 0.46309220951445695 1110 4 0.7564135728271175 1110 5 0.49546373675525146 1110 6 0.7752301312869889 1110 7 1.5910953329893331 1110 8 3.312653508682203 1110 9 -0.5210956717305635 1110 10-11 0.011779407727225077 1110 12-13 0.7700356929715753 1110 14-15 0.7341287238460907 1110 16-17 0.7385800896300196 1110 18-19 1.1437893354153204 1110 20-21 0.08213133819315743 1110 22-23 0.8450324395968352 1110 24-25 0.7748627527024965 1110 26-27 -0.7924446986388745 1110 28-29 0.6343027609245127 1110 30-31 0.6129262073095063 1110 32-33 1.7789976687148155 1110 34-35 1.452429942873131 1110 36-37 0.8162161331057334 1110 38-39 1.2291665086939432 1110 40-41 1.0220209543156358 1110 42-43 1.219443019311072 1110 44-45 2.257849547427675 1110 46-47 1.221385918483513 1110 48-49 1.6284320285721279 1110 50-51 1.131863205385109 1110 52-53 0.36186559097391324 1110 54-55 0.19029054015620517 1110 56-57 1.1560018540599657 1110 58-59 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48-49 0.7618073365381761 2116 50-51 0.541800243436029 2116 52-53 1.0487257059479091 2116 54-55 0.1745226781250011 2116 56-57 0.07997666928033453 2116 58-59 -0.08284709095225651 2116 60-61 -0.9533024142136242 2116 62-63 1.0810276961215521 2116 64-65 1.2146957444113937 2116 66-67 0.15875005657553487 2116 68-69 0.6406752416308876 2116 70-71 -2.1859879928558072 2116 72-73 0.7928965881145302 2116 74-75 -1.7796414999726942 2116 76 -1.849663648562828 2117 1 -0.5212199476577801 2117 2 -0.25874196762747914 2117 3 0.6806210221652194 2117 4 0.2764671777587644 2117 5 -1.3828530683908262 2117 6 -1.8740971268207574 2117 7 -3.0997328632047143 2117 8 -2.5061618223282487 2117 9 -0.1467295500473682 2117 10-11 -0.6801530500588839 2117 12-13 0.5444929430385841 2117 14-15 -0.536458250155512 2117 16-17 -0.21511597040750985 2117 18-19 -0.23418975866133707 2117 20-21 -0.3381581284377688 2117 22-23 -0.03300025941147311 2117 24-25 0.10205653453041919 2117 26-27 1.1569522458800279 2117 28-29 0.36849198633325386 2117 30-31 0.37801331532345017 2117 32-33 0.9319003757638527 2117 34-35 -0.4448040426535371 2117 36-37 -1.6102325401722126 2117 38-39 -0.13558288825057474 2117 40-41 -1.755566823759949 2117 42-43 -1.619458079590025 2117 44-45 0.2050326082692706 2117 46-47 -0.16725519649907028 2117 48-49 -0.46016620246513185 2117 50-51 0.8181403428817546 2117 52-53 0.9017208576584466 2117 54-55 -1.0781897600314068 2117 56-57 -1.0965953048786545 2117 58-59 -0.8116280747458191 2117 60-61 0.7455518519091129 2117 62-63 -0.8841166985862472 2117 64-65 -0.7610566084349628 2117 66-67 -2.3499049460904047 2117 68-69 -0.21104273919915784 2117 70-71 0.6859539268684287 2117 72-73 0.46108015352923815 2117 74-75 0.014678189538386732 2117 76 -0.6845614128938031 2118 1 0.678651180212011 2118 2 0.08121793882090245 2118 3 0.4000258238453256 2118 4 0.5404283251980324 2118 5 -0.2732401620856386 2118 6 0.5425536297063758 2118 7 0.6486085047490846 2118 8 -4.4202232036465645 2118 9 -1.6071968308454245 2118 10-11 -0.8461236481210292 2118 12-13 0.7584445443941306 2118 14-15 -1.2766615817387361 2118 16-17 -0.52533982607072 2118 18-19 -1.5075120344476929 2118 20-21 -2.963205858856366 2118 22-23 -0.9726809429110688 2118 24-25 -0.07572734213417931 2118 26-27 0.3252318029417438 2118 28-29 -0.1671566700554692 2118 30-31 -0.8878272173480255 2118 32-33 0.009814317819127893 2118 34-35 0.6523034939795522 2118 36-37 -0.567155837389528 2118 38-39 -0.12254055136928343 2118 40-41 -1.6478631341985874 2118 42-43 -0.8520901735234929 2118 44-45 -0.3419818540475603 2118 46-47 -0.9537510678178549 2118 48-49 -1.2372054845996345 2118 50-51 -1.223379154994241 2118 52-53 -1.7780975280987974 2118 54-55 -0.13444602464884525 2118 56-57 0.8143158688123364 2118 58-59 0.29628443539689897 2118 60-61 -1.8632436709776279 2118 62-63 0.4143833730093789 2118 64-65 -0.3766000192506809 2118 66-67 -2.11597816455253 2118 68-69 1.149023606082693 2118 70-71 -1.3713643395627741 2118 72-73 -2.1474826835668424 2118 74-75 0.10173250675475032 2118 76 -0.9739628734446129 2119 1 0.49796924840519097 2119 2 0.4963764229724852 2119 3 0.461744651962519 2119 4 0.23048431959859172 2119 5 0.7397085430438501 2119 6 0.48823406166317795 2119 7 -1.8257940549949438 2119 8 0.2798067933537354 2119 9 -1.2986026902594787 2119 10-11 -0.113346925793266 2119 12-13 0.2837920096476054 2119 14-15 -0.9548437635205573 2119 16-17 -0.9154008199713317 2119 18-19 -1.6881814675043856 2119 20-21 -1.729266752766975 2119 22-23 0.26337045194944864 2119 24-25 -0.5557043444339484 2119 26-27 0.20345648047398868 2119 28-29 0.405223591918336 2119 30-31 -0.11459204086567354 2119 32-33 -0.03179402134695408 2119 34-35 0.18904981934701937 2119 36-37 -0.30075747722554524 2119 38-39 -0.6976330421202093 2119 40-41 -1.3485180687051397 2119 42-43 -2.032909591581685 2119 44-45 -2.423823669865982 2119 46-47 -0.5891250304215987 2119 48-49 -1.6564135637917161 2119 50-51 0.23938706838342227 2119 52-53 -2.1514976880827987 2119 54-55 -2.258446124638848 2119 56-57 0.48492380801841506 2119 58-59 1.176958867953637 2119 60-61 0.6254699576595044 2119 62-63 -0.3009825903942769 2119 64-65 -0.362076471605441 2119 66-67 0.4150687307579375 2119 68-69 -0.9824382477319205 2119 70-71 -1.4300459713995863 2119 72-73 -2.6355588759476056 2119 74-75 -0.5425905609384785 2119 76 0.8786268675812892 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 5.0 13 80.0 14 236.0 15 456.0 16 608.0 17 741.0 18 818.0 19 793.0 20 825.0 21 793.0 22 826.0 23 923.0 24 1156.0 25 1388.0 26 1629.0 27 1919.0 28 2361.0 29 2901.0 30 3541.0 31 4414.0 32 5582.0 33 7329.0 34 9798.0 35 14606.0 36 23882.0 37 27163.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.309596979541666 21.63315479848977 11.359206251646325 30.698041970322244 2 18.317025781324876 19.527240727348765 36.81527885478292 25.340454636543434 3 17.652235281817152 25.052930567293703 28.772446481315296 28.522387669573856 4 13.165117231404599 17.538096939175592 37.893929756998595 31.402856072421216 5 12.716405426363341 36.37092347503333 35.074451308234515 15.838219790368816 6 28.315893110749045 38.67111602903122 17.112909830709313 15.900081029510424 7 27.47597431451648 29.038188424106714 23.42972650362019 20.056110757756613 8 23.46370662089516 33.69259320571911 20.371515948872993 22.472184224512734 9 23.152657855070444 16.35924825525167 22.276145086387913 38.21194880328997 10-11 24.626872173769094 25.476375105643314 27.54044940883309 22.35630331175451 12-13 24.024378555932145 24.006952854765494 26.52975874116735 25.438909848135015 14-15 21.647948559330157 25.220217298493548 24.40164498619013 28.730189155986164 16-17 21.80739372500501 27.979577078232687 26.73494637240466 23.478082824357646 18-19 21.279394979655493 27.238549136120866 27.389281451212394 24.09277443301125 20-21 21.06288064265986 27.75173603547873 27.23201449818337 23.95336882367804 22-23 22.474362437158565 27.02639122441689 27.672449095170464 22.826797243254077 24-25 21.67539403866763 27.873280301116115 27.091301961262666 23.360023698953587 26-27 20.52486211913952 28.5476549362655 27.802270568862014 23.125212375732968 28-29 21.69281973983428 27.41542000296237 28.769396983611127 22.12236327359222 30-31 21.522047868401103 27.560488965174734 27.91030991609525 23.00715325032891 32-33 20.795831772280938 28.14947766460753 27.705557927387105 23.34913263572443 34-35 21.960739895271537 27.518667282374775 28.12377475538672 22.39681806696697 36-37 22.31491727148371 27.44199419724151 27.238549136120866 23.004539395153913 38-39 21.32905822798045 27.612766068674688 28.6160508133446 22.44212489000026 40-41 21.72897806975508 26.266195011021754 28.75589206520697 23.248934854016188 42-43 21.065930140364024 26.644768368867243 28.574229130544644 23.71507236022409 44-45 20.364545668406333 26.61253082170894 28.74151586174449 24.28140764814024 46-47 21.071157850714016 25.764334817422217 29.262544326627342 23.901963005236425 48-49 20.240823190123113 26.27752171678008 29.830622184660154 23.651032908436655 50-51 20.114486856664897 26.237006961567616 29.525236771714603 24.123269410052885 52-53 19.502844745715457 26.240492101800942 29.759176809876887 24.497486342606713 54-55 18.71215355527868 26.956252777221124 29.575771305097888 24.75582236240231 56-57 19.138647591332454 26.842985719637895 29.31177193242313 24.70659475660652 58-59 19.377379697315572 26.687461336725537 28.777674191665287 25.157484774293604 60-61 19.613497948123687 26.45831336638408 28.676169482369545 25.252019203122682 62-63 19.414409312294705 26.173403152309344 29.085673459785838 25.326514075610117 64-65 20.266526099343924 26.427382746813276 27.915537626445243 25.390553527397557 66-67 19.934566492119227 26.141601247680203 27.633676910074666 26.2901553501259 68-69 19.937180347294227 25.893720648584594 27.605360145678866 26.563738858442314 70-71 20.661653873297727 25.931621548622065 26.918787519712822 26.487937058367383 72-73 20.92870274367665 25.3796624641684 26.769362132208798 26.922272659946156 74-75 21.13171216226813 25.085603756981172 26.884807402437854 26.897876678312844 76 21.485889538480304 24.469169578210902 27.137044426825124 26.907896456483666 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 0.5 11 1.0 12 1.0 13 0.5 14 0.0 15 2.0 16 4.0 17 4.0 18 6.5 19 9.5 20 13.0 21 16.0 22 23.0 23 44.5 24 64.0 25 69.0 26 150.5 27 273.5 28 399.5 29 484.0 30 618.5 31 892.5 32 1360.5 33 1689.0 34 1910.5 35 2461.0 36 3091.5 37 3393.0 38 3952.0 39 5123.5 40 6418.5 41 7427.5 42 7754.0 43 7813.5 44 8368.0 45 8857.0 46 8851.0 47 9048.0 48 8741.0 49 7829.5 50 7422.0 51 6955.0 52 5867.5 53 4598.5 54 3950.0 55 3671.5 56 2937.0 57 2066.0 58 1651.0 59 1319.5 60 848.5 61 560.5 62 412.0 63 335.5 64 208.0 65 133.5 66 93.0 67 76.0 68 71.0 69 51.0 70 29.5 71 23.0 72 22.5 73 15.0 74 9.5 75 11.0 76 10.0 77 8.0 78 5.5 79 4.0 80 6.0 81 6.5 82 5.5 83 6.0 84 5.0 85 3.5 86 3.0 87 3.0 88 2.0 89 2.0 90 2.5 91 2.0 92 2.0 93 2.0 94 2.0 95 1.0 96 0.0 97 0.5 98 0.5 99 3.5 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7693447065076281 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 114773.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.62578306744618 #Duplication Level Percentage of deduplicated Percentage of total 1 75.82211141738505 37.62731652914884 2 8.581912670962305 8.517682730258858 3 4.520954404199659 6.730677075618831 4 2.735396878346823 5.429848483528356 5 1.8101374721280965 4.491474475704217 6 1.1394560809031375 3.3927840171468904 7 0.9673964569763155 3.360546469988586 8 0.7637340449813017 3.0320720029972206 9 0.5986972628474112 2.673973844022549 >10 3.040890496339344 24.176417798611173 >50 0.019312814930561653 0.5672065729744801 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.007841565524992811 0.0 2 0.0 0.0 0.0 0.008712850583325346 0.0 3 0.0 0.0 0.0 0.008712850583325346 0.0 4 0.0 0.0 0.0 0.010455420699990416 0.0 5 0.0 0.0 0.0 0.010455420699990416 0.0 6 0.0 0.0 0.0 0.010455420699990416 0.0 7 0.0 0.0 0.0 0.01132670575832295 0.0 8 0.0 0.0 0.0 0.01132670575832295 0.0 9 0.0 0.0 0.0 0.01655441610831816 0.0 10 0.0 0.0 0.0 0.020039556341648296 0.0 11 0.0 0.0 0.0 0.020910841399980833 0.0 12 0.0 0.0 0.0 0.0226534115166459 0.0 13 0.0 0.0 0.0 0.02613855174997604 0.0 14 0.0 0.0 0.0 0.027009836808308575 0.0 15 0.0 0.0 0.0 0.028752406924973644 0.0 16 0.0 0.0 0.0 0.029623691983306177 0.0 17 0.0 0.0 0.0 0.03310883221663632 0.0 18 0.0 0.0 0.0 0.03485140233330138 0.0 19 0.0 0.0 0.0 0.036593972449966455 0.0 20 0.0 0.0 0.0 0.03920782762496406 0.0 21 0.0 0.0 0.0 0.04356425291662673 0.0 22 0.0 0.0 0.0 0.04792067820828941 0.0 23 0.0 0.0 0.0 0.04879196326662194 0.0 24 0.0 0.0 0.0 0.051405818441619545 0.0 25 0.0 0.0 0.0 0.05489095867494968 0.0 26 0.0 0.0 0.0 0.05924738396661235 0.0 27 0.0 0.0 0.0 0.07580180007493051 0.0 28 0.0 0.0 0.0 0.10629677711656922 0.0 29 0.0 0.0 0.0 0.13504918404154287 0.0 30 0.0 0.0 0.0 0.16205902084985144 0.0 31 0.0 0.0 0.0 0.19516785306648776 0.0 32 0.0 0.0 0.0 0.23176182551645422 0.0 33 0.0 0.0 0.0 0.2805537887830762 0.0 34 0.0 0.0 0.0 0.32063290146637274 0.0 35 0.0 0.0 0.0 0.41124654753295636 0.0 36 0.0 0.0 0.0 0.541068021224504 0.0 37 0.0 0.0 0.0 0.8381762261158984 0.0 38 0.0 0.0 0.0 1.2407099230655294 0.0 39 0.0 0.0 0.0 1.733857266081744 0.0 40 0.0 0.0 0.0 2.2958361287062288 0.0 41 0.0 0.0 0.0 3.0233591524138954 0.0 42 0.0 0.0 0.0 3.804901849738179 0.0 43 0.0 0.0 0.0 4.63349394021242 0.0 44 0.0 0.0 0.0 5.415036637536703 0.0 45 0.0 0.0 0.0 6.326400808552534 0.0 46 0.0 0.0 0.0 7.230794699101705 0.0 47 0.0 0.0 0.0 8.114277748250895 0.0 48 0.0 0.0 0.0 8.943741123783468 0.0 49 0.0 0.0 0.0 9.811541041882673 0.0 50 0.0 0.0 0.0 10.619222290956932 0.0 51 0.0 0.0 0.0 11.44345795613951 0.0 52 0.0 0.0 0.0 12.297317313305394 0.0 53 0.0 0.0 0.0 13.20693891420456 0.0 54 0.0 0.0 0.0 14.006778597753827 0.0 55 0.0 0.0 0.0 14.756955032978139 0.0 56 0.0 0.0 0.0 15.491448337152466 0.0 57 0.0 0.0 0.0 16.209387225218475 0.0 58 0.0 0.0 0.0 16.97786064666777 0.0 59 0.0 0.0 0.0 17.701027245083775 0.0 60 0.0 0.0 0.0 18.452074965366418 0.0 61 0.0 0.0 0.0 19.190053409774077 0.0 62 0.0 0.0 0.0 19.90973486795675 0.0 63 0.0 0.0 0.0 20.67298057905605 0.0 64 0.0 0.0 0.0 21.359553205022088 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTGGCC 15 0.0022205578 69.9939 35 ATCCGGC 15 0.0022205578 69.9939 9 GTTACCT 15 0.0022205578 69.9939 15 AGCCTTA 15 0.0022205578 69.9939 65 GCCCAGC 20 0.0069385716 52.495422 39 GTTCTGC 20 0.0069385716 52.495422 24 GGTCGTT 20 0.0069385716 52.495422 11 GAAGATA 20 0.0069385716 52.495422 14 ATTAACG 20 0.0069385716 52.495422 39 TGAAAGT 20 0.0069385716 52.495422 29 GGCCCAG 20 0.0069385716 52.495422 38 CGTTACC 20 0.0069385716 52.495422 14 ACCTAGA 20 0.0069385716 52.495422 18 GCCTGGC 20 0.0069385716 52.495422 30 GTCGTTA 20 0.0069385716 52.495422 12 TACCTAG 20 0.0069385716 52.495422 17 TGCCTGG 20 0.0069385716 52.495422 29 CTCGCTT 20 0.0069385716 52.495422 16 CATCGTT 20 0.0069385716 52.495422 32 GAGTTAC 20 0.0069385716 52.495422 61 >>END_MODULE