FastQCFastQC Report
Wed 25 May 2016
SRR1780638_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780638_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114773
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT6400.5576224373328222No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT3290.28665278419140394RNA PCR Primer, Index 47 (95% over 21bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1490.12982147369154765No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGCT150.002219790170.015
CCTTGGG150.002219790170.014
GTCATCA252.3819463E-456.0000049
AACGTAA252.3819463E-456.00000446
AACAACG252.3819463E-456.00000443
CACTGAC252.3819463E-456.00000454
CTGACAT252.3819463E-456.00000456
GACTTTC252.3819463E-456.00000463
GACATGA252.3819463E-456.00000458
CGTAAAA252.3819463E-456.00000448
TAATCTA252.3819463E-456.0000045
GTGCAAC200.006936180452.523
GACAGTT200.006936180452.562
GCCTTTC200.006936180452.57
GCGACAG200.006936180452.514
CATGCAA200.006936180452.537
GCTGGGC200.006936180452.537
GGCGTTC200.006936180452.540
TGGCGTG200.006936180452.523
GATTTCC200.006936180452.537
Produced by FastQC (version 0.11.5)