FastQCFastQC Report
Wed 25 May 2016
SRR1780637_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780637_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences984861
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAC207.916066E-443.9319153
CGAGTTC250.002334721235.25471514
AGATAGC250.00236521235.16160244
GAGTTCC300.005693964729.38641415
ACGTGAG300.005729492329.34903735
ATAAGTC300.005729492329.34903735
CGTTCCA454.075105E-529.32665625
CGAGAAC300.005750892729.32665625
CACGTCC300.005788136329.2879473
AGGTACT804.3855835E-927.4574494
TAGGTAC804.3855835E-927.4574493
CAAGGAA1550.026.9260164
GTACAAG1600.026.1124151
GCATAAG1151.2732926E-1124.8575861
TCAATCC801.2885175E-724.79099721
CCGAGTT450.001386256324.4799513
GCCGAGT450.001387079124.47745712
TACGGGT450.001393675724.45753137
GGTACGG450.001406946624.4177844
CGTGCGA1450.024.29009610
Produced by FastQC (version 0.11.5)