FastQCFastQC Report
Wed 25 May 2016
SRR1780637_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780637_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences984861
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT99451.0097871679353736No Hit
ATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTT35180.35720776840589685TruSeq Adapter, Index 10 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC16100.163474845688884No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC11820.12001693640016206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT4300.035.8160244
GCCGTCT4750.032.42292443
GGACACT300.005744308229.3335386
TTTATAA300.005747159529.3305629
TCTTCAG1700.027.17389716
TATGCCG16100.026.92080743
TCTGGTA1006.002665E-1126.40286312
ATGCCGT16500.026.00150144
TATACCG1800.025.6668456
CTTCAGC1850.024.9706117
CGAGAAT1151.2732926E-1124.87226314
ACTTTGC1600.024.74891538
CGTGCGA1251.8189894E-1224.64267310
GTATACG450.001397559924.44585444
CCCAACG551.5937997E-423.99894721
CGTTGTT551.5943487E-423.99772834
CTCGTAT17950.023.89988539
CGTATGC18150.023.88016541
TCTCGTA17850.023.7860738
TCGTATG18100.023.70302640
Produced by FastQC (version 0.11.5)