FastQCFastQC Report
Wed 25 May 2016
SRR1780636_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780636_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54100
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATAGC150.002214358269.9926150
ACTCGCC150.002214358269.9926145
TTCAGTG200.00691935152.49445713
CTGAGTC200.00691935152.49445769
TTAGAGT200.00691935152.4944574
TATCTGA200.00691935152.49445755
TGTTCCC200.00691935152.4944572
CTCGCCA200.00691935152.49445746
TGTAGGG200.00691935152.4944574
TAATGGC200.00691935152.49445726
CCCAACT305.832627E-446.66173623
TAGTTGT350.001245840539.99577323
TCACTTT350.001245840539.99577327
GTTATGT400.002314452335.2569851
AATAGCC400.002400424834.99630451
CGGAGGC400.002400424834.99630427
ATAGCCC450.00427482531.10782452
TGTAAGG450.00427482531.1078242
CAATACT450.00427482531.1078244
ACGAAAG450.00427482531.10782437
Produced by FastQC (version 0.11.5)