##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780636_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 54100 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.119944547134935 34.0 27.0 34.0 12.0 34.0 2 30.689038817005546 34.0 32.0 34.0 21.0 34.0 3 30.975711645101665 34.0 32.0 34.0 21.0 34.0 4 31.397670979667282 34.0 34.0 34.0 23.0 34.0 5 31.34040665434381 34.0 34.0 34.0 23.0 34.0 6 34.18345656192236 38.0 35.0 38.0 23.0 38.0 7 33.33072088724584 37.0 34.0 38.0 21.0 38.0 8 32.10475046210721 37.0 31.0 38.0 11.0 38.0 9 32.8962292051756 37.0 33.0 38.0 12.0 38.0 10-11 33.31220887245841 37.0 34.0 38.0 21.0 38.0 12-13 33.6524584103512 37.0 34.0 38.0 21.0 38.0 14-15 33.269343807763406 37.0 34.0 38.0 21.0 38.0 16-17 33.16587800369686 37.0 34.0 38.0 21.0 38.0 18-19 33.102107208872454 37.0 33.5 38.0 16.0 38.0 20-21 33.16308687615526 37.0 34.0 38.0 21.0 38.0 22-23 33.160720887245844 37.0 34.0 38.0 21.0 38.0 24-25 33.64029574861368 37.0 34.0 38.0 21.0 38.0 26-27 33.74666358595194 37.5 34.0 38.0 21.0 38.0 28-29 33.863133086876154 37.5 34.0 38.0 21.5 38.0 30-31 33.70049907578559 37.0 34.0 38.0 21.0 38.0 32-33 33.47547134935305 37.0 34.0 38.0 21.0 38.0 34-35 33.29830868761553 37.0 34.0 38.0 21.0 38.0 36-37 33.27312384473198 37.0 34.0 38.0 21.0 38.0 38-39 33.82781885397412 37.0 34.0 38.0 21.5 38.0 40-41 33.293049907578556 37.0 34.0 38.0 21.0 38.0 42-43 33.655341959334564 37.0 34.0 38.0 21.0 38.0 44-45 32.67482439926063 37.0 31.5 38.0 16.0 38.0 46-47 33.353798521256934 37.0 34.0 38.0 21.0 38.0 48-49 33.10929759704251 37.0 33.5 38.0 21.0 38.0 50-51 33.07052680221811 37.0 33.0 38.0 21.0 38.0 52-53 33.035378927911275 37.0 33.0 38.0 21.0 38.0 54-55 32.74087800369686 37.0 32.0 38.0 16.0 38.0 56-57 32.82396487985213 37.0 32.0 38.0 16.0 38.0 58-59 32.64770794824399 37.0 31.5 38.0 16.0 38.0 60-61 32.70544362292051 37.0 31.5 38.0 15.5 38.0 62-63 32.78343807763401 37.0 32.0 38.0 19.5 38.0 64-65 32.74301293900185 37.0 31.5 38.0 15.0 38.0 66-67 32.73289279112754 37.0 31.5 38.0 18.5 38.0 68-69 32.272347504621074 37.0 31.0 38.0 15.0 38.0 70-71 32.153345656192236 37.0 31.0 38.0 11.0 38.0 72-73 32.27203327171904 37.0 31.0 38.0 11.0 38.0 74-75 31.845776340110906 37.0 30.0 38.0 11.0 38.0 76 30.944768946395563 36.0 27.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.6210659308487223 1101 2 -0.18451984503598595 1101 3 -0.6036022931966194 1101 4 -3.0023541720552984 1101 5 -0.5054190516108008 1101 6 -1.2234664595106963 1101 7 -1.7206474151838904 1101 8 -0.9109346884925564 1101 9 -0.09391400185263876 1101 10-11 -2.2812289660707457 1101 12-13 -1.7142210422865816 1101 14-15 -1.3288286661872704 1101 16-17 -1.6699930534378318 1101 18-19 -0.29085867333989057 1101 20-21 -1.37455394351867 1101 22-23 -0.6408370760917848 1101 24-25 -1.7713682389257528 1101 26-27 -1.228062406920877 1101 28-29 -0.6139700080285877 1101 30-31 -1.2083520301674753 1101 32-33 0.3962047582160366 1101 34-35 -0.38828090645244373 1101 36-37 -0.047117181454190415 1101 38-39 -1.1054801945077202 1101 40-41 -0.0444537135230334 1101 42-43 -0.15130759477595745 1101 44-45 -0.4810533978506655 1101 46-47 -0.33927174136892546 1101 48-49 -0.8259911799539239 1101 50-51 -1.2943332816153799 1101 52-53 -0.3015373336868308 1101 54-55 -1.2458877621045161 1101 56-57 -0.1488814144209769 1101 58-59 -0.25859734242223453 1101 60-61 0.5936635405244886 1101 62-63 -1.5589034731054774 1101 64-65 6.378390860248828E-4 1101 66-67 -1.4775547181732591 1101 68-69 -0.9494711851804851 1101 70-71 0.4970639311100413 1101 72-73 -0.9085170467252155 1101 74-75 -1.347675998188116 1101 76 -2.215627052107852 1102 1 -0.7510459520027943 1102 2 -1.0598871032331871 1102 3 -0.7804150796580132 1102 4 -0.19990970149471465 1102 5 -0.42838297216550814 1102 6 0.3250116225200941 1102 7 1.9400060106005697 1102 8 1.5377666865221329 1102 9 1.4938753976067574 1102 10-11 -0.6962307289100877 1102 12-13 -4.455759413423024 1102 14-15 -1.7944826795848492 1102 16-17 -2.0995088602306424 1102 18-19 -1.4387608945174648 1102 20-21 -0.19101298688451607 1102 22-23 -0.6630194712028441 1102 24-25 0.3521480379575479 1102 26-27 -5.417508875366053 1102 28-29 -1.158893030710459 1102 30-31 0.020258852427424756 1102 32-33 -7.041684535472999 1102 34-35 -1.1532650408983258 1102 36-37 -0.014886602067662125 1102 38-39 0.39813362615275594 1102 40-41 0.11855016472352986 1102 42-43 -0.3795659095015438 1102 44-45 -5.747741610331566 1102 46-47 -0.658933276214384 1102 48-49 0.5088601539278557 1102 50-51 -6.301884110149871 1102 52-53 -2.5290317513622327 1102 54-55 -0.10224573601449549 1102 56-57 -2.1187956229061093 1102 58-59 0.1744441430637238 1102 60-61 0.10268340184779845 1102 62-63 -4.48213181956217 1102 64-65 -1.0127597399479384 1102 66-67 -0.19267987976686385 1102 68-69 -5.8063286302386565 1102 70-71 -0.13617734283519667 1102 72-73 -0.436164243810655 1102 74-75 -6.144538144151213 1102 76 -2.439625171745881 1103 1 0.3810676734184888 1103 2 -0.2567575895114729 1103 3 0.049483812975591235 1103 4 0.7854538035606495 1103 5 1.0383960360193711 1103 6 -1.462277737133256 1103 7 0.4607089475288362 1103 8 0.9761874857517938 1103 9 1.1064415988585594 1103 10-11 0.8933455830783501 1103 12-13 -1.5881859902164805 1103 14-15 -1.0745741576782564 1103 16-17 -0.8456090046697398 1103 18-19 -0.10231409625072985 1103 20-21 -0.4656639257386317 1103 22-23 0.09858380274997103 1103 24-25 0.3073719185545656 1103 26-27 -0.8702772913506465 1103 28-29 0.5564897328908884 1103 30-31 0.16929592512843072 1103 32-33 -5.053528541250564 1103 34-35 -1.575580881052396 1103 36-37 -0.5227585327368942 1103 38-39 0.4360488885504452 1103 40-41 0.5998693751231485 1103 42-43 0.3464572007536333 1103 44-45 -5.145213926171721 1103 46-47 -0.06726259735063422 1103 48-49 0.16420440043724227 1103 50-51 0.33761998229115164 1103 52-53 0.33757874454532555 1103 54-55 1.1637388571487222 1103 56-57 1.0293507420433414 1103 58-59 0.8453396654517817 1103 60-61 1.7054999641973367 1103 62-63 0.21948108366363783 1103 64-65 1.3865662109427674 1103 66-67 0.5365931101223964 1103 68-69 -2.2286685435751963 1103 70-71 0.42340378378974464 1103 72-73 1.0087803878696562 1103 74-75 -1.6258573545508277 1103 76 -0.29990442066258893 1104 1 -1.573307194808372 1104 2 0.3581696717155971 1104 3 0.12374061246958235 1104 4 0.5211906789559748 1104 5 -0.8719982292136024 1104 6 -0.5643650236607698 1104 7 0.6983222724286833 1104 8 2.239649429669143 1104 9 0.053479338685676225 1104 10-11 0.15073542073384516 1104 12-13 -0.36549992654369845 1104 14-15 0.03780197557074416 1104 16-17 -0.32819386657148186 1104 18-19 0.3120439050142849 1104 20-21 0.7089723803967232 1104 22-23 0.366367905638441 1104 24-25 0.13743727803169037 1104 26-27 0.3213946462415933 1104 28-29 -0.6419922405436438 1104 30-31 -1.5328335288036712 1104 32-33 -1.4442306285370918 1104 34-35 -0.17792117200749402 1104 36-37 0.18004559966875178 1104 38-39 -0.7272443857485271 1104 40-41 0.8490154851800753 1104 42-43 0.2312769350989896 1104 44-45 -3.0428103841097354 1104 46-47 0.3150692601235363 1104 48-49 0.06814705276698874 1104 50-51 0.6470233135161081 1104 52-53 -0.8431718352564843 1104 54-55 -0.4326769134605968 1104 56-57 1.0162155955116035 1104 58-59 0.9446860706805396 1104 60-61 0.13514364962850323 1104 62-63 0.414927003967243 1104 64-65 -0.18766950484892675 1104 66-67 -0.07932508511268566 1104 68-69 -2.9707516133622534 1104 70-71 -0.5498199142916391 1104 72-73 0.2503785325038521 1104 74-75 -0.7671603273528511 1104 76 -0.7491559492310067 1105 1 -0.3030049072266756 1105 2 0.31380692661755916 1105 3 -0.24991134831472905 1105 4 0.5515828358187136 1105 5 0.9023073263419548 1105 6 1.1473996822215824 1105 7 2.289539592690126 1105 8 2.165098776074373 1105 9 2.051804502084366 1105 10-11 1.9748980024331857 1105 12-13 -0.3549198611842215 1105 14-15 1.1161516487733536 1105 16-17 1.7158829308141321 1105 18-19 1.3555488069750723 1105 20-21 0.22465865490652703 1105 22-23 1.203724931782233 1105 24-25 1.7073719185545642 1105 26-27 1.204319482842898 1105 28-29 0.3997357006328244 1105 30-31 1.4571991509348905 1105 32-33 1.9195561361687936 1105 34-35 1.8628263770121194 1105 36-37 1.7064753382308453 1105 38-39 1.487359372421416 1105 40-41 1.987066955768313 1105 42-43 1.6514975233342852 1105 44-45 2.802165106086342 1105 46-47 1.906225305875168 1105 48-49 1.4438414972114302 1105 50-51 0.8526401435814748 1105 52-53 0.43062309938403587 1105 54-55 0.3582953087616261 1105 56-57 0.9310644517207578 1105 58-59 1.1203396654517803 1105 60-61 1.1783830287134691 1105 62-63 1.65849318043783 1105 64-65 1.2062234690072842 1105 66-67 1.2387100456062612 1105 68-69 2.778186295134475 1105 70-71 2.646891687015547 1105 72-73 1.7640223233535224 1105 74-75 0.2400700648040086 1105 76 -0.20716248517871705 1106 1 0.39424159626982913 1106 2 -0.120371395060765 1106 3 -0.005287222440603756 1106 4 -0.330584996349117 1106 5 0.3968440396286681 1106 6 -0.187565352743448 1106 7 1.1399329493334776 1106 8 0.2409291956547932 1106 9 1.4937188377487018 1106 10-11 0.2764495758597647 1106 12-13 0.5896168521024947 1106 14-15 0.3006796208013327 1106 16-17 0.8361189448001483 1106 18-19 0.634613492289752 1106 20-21 0.43864466889254317 1106 22-23 0.4295334982157968 1106 24-25 0.6605187717014189 1106 26-27 0.8391971052205278 1106 28-29 -0.9379609776888529 1106 30-31 -0.41150714277140565 1106 32-33 0.44881749980515195 1106 34-35 0.6905143140750525 1106 36-37 1.2616763871819003 1106 38-39 1.0101522045892395 1106 40-41 0.3496325152088673 1106 42-43 0.5720831876699464 1106 44-45 1.5051808403520752 1106 46-47 1.338196459721324 1106 48-49 0.8902138748338047 1106 50-51 1.1735098988262322 1106 52-53 0.37653656092250287 1106 54-55 0.642692161908478 1106 56-57 1.0604132279445295 1106 58-59 0.2628221829343005 1106 60-61 1.1349608259162665 1106 62-63 1.390245802815457 1106 64-65 1.2526614060702173 1106 66-67 1.201537842809067 1106 68-69 1.126350630798811 1106 70-71 1.2386530506519193 1106 72-73 1.2660328128640188 1106 74-75 -0.4945016135176701 1106 76 -0.030064583080815055 1107 1 -0.03330554013806619 1107 2 -0.39362978224320244 1107 3 0.14099055041944553 1107 4 0.0015828358187164326 1107 5 0.03798770608878854 1107 6 0.13220980880386435 1107 7 0.6630364281331609 1107 8 1.0048930798718416 1107 9 -0.8419454979156313 1107 10-11 -0.32862256718706107 1107 12-13 0.2592415312208445 1107 14-15 -0.9340065790747403 1107 16-17 0.04681647511793585 1107 18-19 0.09486842722824207 1107 20-21 0.4657978954128552 1107 22-23 -0.5989253846734641 1107 24-25 -0.6673116257492353 1107 26-27 -0.17242102348621557 1107 28-29 -0.8988798056962892 1107 30-31 -0.4975476845081559 1107 32-33 0.5472064526244864 1107 34-35 -0.23072583817775438 1107 36-37 -0.5056290921489008 1107 38-39 0.38684513191508785 1107 40-41 -0.09904855056080919 1107 42-43 0.2621779030811098 1107 44-45 -0.5170595774579638 1107 46-47 -0.2207129852640719 1107 48-49 -0.3608262243075586 1107 50-51 0.8169993207966613 1107 52-53 0.7915803778650456 1107 54-55 0.1597984733185811 1107 56-57 0.6955818567840524 1107 58-59 0.033314348996086096 1107 60-61 -0.6527483004004608 1107 62-63 -1.0074482752583727 1107 64-65 -0.4913635563091674 1107 66-67 0.6923097291505727 1107 68-69 0.8729647761471355 1107 70-71 -0.2515655914654644 1107 72-73 0.16576282968264167 1107 74-75 0.25344031796856825 1107 76 -0.23595995353315047 1108 1 -0.030823534677654862 1108 2 0.16209124034304878 1108 3 -0.48410252478531746 1108 4 -0.4592014779067739 1108 5 -0.21840345797177108 1108 6 -0.6689401870594622 1108 7 0.24080593255940386 1108 8 -0.19825906706288166 1108 9 -1.835409550203213 1108 10-11 -0.636846278612559 1108 12-13 -0.4733430637986018 1108 14-15 0.0933575311263013 1108 16-17 -1.26610236330351 1108 18-19 -0.03436132374388734 1108 20-21 0.05864558301110634 1108 22-23 -0.839514447302733 1108 24-25 -1.5161574932101374 1108 26-27 -0.8158602557191941 1108 28-29 -0.23349550851749967 1108 30-31 -0.04263745037230393 1108 32-33 0.444658260351801 1108 34-35 0.0018173900840139368 1108 36-37 0.2356011552243089 1108 38-39 0.3446510390880846 1108 40-41 -0.08889301155195284 1108 42-43 0.7410808566676153 1108 44-45 -0.5754901226718303 1108 46-47 -0.46597649151044607 1108 48-49 -0.647539221742818 1108 50-51 0.25813442462722236 1108 52-53 -0.2908842535571381 1108 54-55 -0.02744815529066358 1108 56-57 0.10445088962925553 1108 58-59 -1.7187126221299174 1108 60-61 0.42926129668732216 1108 62-63 0.4574106640979636 1108 64-65 -0.30204858981624483 1108 66-67 -1.2982793334786997 1108 68-69 0.46388890951355677 1108 70-71 1.2018140726364663 1108 72-73 0.11312363054306473 1108 74-75 0.37989849617656546 1108 76 0.6233930703768387 1109 1 -0.9233574713292398 1109 2 -1.3627956374850072 1109 3 0.17012070296732063 1109 4 -0.5176479334120536 1109 5 -0.5728529300683007 1109 6 -0.02439518957328346 1109 7 -0.6013257919252624 1109 8 0.8341692888948842 1109 9 1.1516442456741132 1109 10-11 1.0299460793562716 1109 12-13 -0.23584934836370763 1109 14-15 -0.18096373584202752 1109 16-17 -0.6682516845704782 1109 18-19 0.1480167556930212 1109 20-21 1.0688894241373035 1109 22-23 0.3086127522950477 1109 24-25 0.09903858522122988 1109 26-27 -0.16779590177248593 1109 28-29 -0.4263059660338442 1109 30-31 -0.29728802855229475 1109 32-33 0.8191554951431499 1109 34-35 -0.1792409306801872 1109 36-37 -0.4026592771537665 1109 38-39 -0.4341951147580758 1109 40-41 0.43762785320420505 1109 42-43 0.5039013694881191 1109 44-45 -0.027161816990577847 1109 46-47 -0.5944958479709825 1109 48-49 -0.9233059386860063 1109 50-51 0.11698309229942083 1109 52-53 0.5720493814353205 1109 54-55 -0.10468546046914895 1109 56-57 0.02777919531050088 1109 58-59 0.20687812699024732 1109 60-61 0.4595529005083421 1109 62-63 -0.5334138708442211 1109 64-65 -0.29337588996707353 1109 66-67 0.2665145327857559 1109 68-69 0.6464555259037041 1109 70-71 -0.5672910052921409 1109 72-73 -1.4542469074157083 1109 74-75 0.8568969878809298 1109 76 -0.8680599210761528 1110 1 0.6487068762128168 1110 2 -0.14901154472002176 1110 3 -0.06746708079880648 1110 4 0.3168694600225379 1110 5 0.6198630588260308 1110 6 0.7072722936865503 1110 7 1.4966653888684718 1110 8 2.3818583302144987 1110 9 -1.249588810017542 1110 10-11 -0.8001617109426107 1110 12-13 -0.06316030704409314 1110 14-15 -0.05988338307377461 1110 16-17 0.1788208289033122 1110 18-19 0.9238210999686984 1110 20-21 -0.14222032598518553 1110 22-23 0.06055216108158845 1110 24-25 0.6481362497647609 1110 26-27 -1.9615244662016842 1110 28-29 0.272207834390791 1110 30-31 0.2070399152661011 1110 32-33 1.6647989705637016 1110 34-35 1.2024959629993859 1110 36-37 -0.13122370635513647 1110 38-39 0.5028251367526266 1110 40-41 -0.23847046461385446 1110 42-43 0.6816328736527524 1110 44-45 1.401488354493985 1110 46-47 0.5569617708433299 1110 48-49 1.6049481851095209 1110 50-51 0.3386473091865696 1110 52-53 0.6092656153076064 1110 54-55 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2.248856282556929 2116 46-47 0.7321444235222287 2116 48-49 1.1155326736820186 2116 50-51 0.5947357318167619 2116 52-53 1.598821628795804 2116 54-55 -0.4714841030030854 2116 56-57 -0.5687517247498306 2116 58-59 -0.1517191580776327 2116 60-61 -1.8681243242277112 2116 62-63 0.933309356908417 2116 64-65 1.4332455278308132 2116 66-67 0.20212916325332486 2116 68-69 0.7759804127815357 2116 70-71 -2.0183656659256215 2116 72-73 0.8610811468829382 2116 74-75 -0.871326994019519 2116 76 0.23695516188010401 2117 1 -0.2264424072266742 2117 2 -0.11119307338244155 2117 3 1.0797761516852695 2117 4 0.45470783581871643 2117 5 -1.1523801736580452 2117 6 -1.1604128177784148 2117 7 -2.7870229073098756 2117 8 -1.1864637239256268 2117 9 0.6189920020843687 2117 10-11 -0.17588324756680862 2117 12-13 0.47398638881578137 2117 14-15 -0.7729108512266407 2117 16-17 -0.15052331918586503 2117 18-19 -0.1639824430249277 2117 20-21 -0.4425288450934701 2117 22-23 0.3201311817822301 2117 24-25 0.5222156685545656 2117 26-27 0.9004132328429009 2117 28-29 0.3262982006328272 2117 30-31 0.28844915093488765 2117 32-33 0.8508061361687922 2117 34-35 0.058138877012119394 2117 36-37 -1.5021184117691533 2117 38-39 -0.032953127578586816 2117 40-41 -2.4519955442316927 2117 42-43 -2.0024087266657205 2117 44-45 0.20841510608634195 2117 46-47 -0.04221219412482924 2117 48-49 -0.8506897527885684 2117 50-51 0.46514014358147193 2117 52-53 1.4853105993840359 2117 54-55 -0.8198296912383753 2117 56-57 -1.2001855482792436 2117 58-59 0.17502716545178032 2117 60-61 -0.2950544712865337 2117 62-63 0.13896193043783 2117 64-65 -0.24299528099271583 2117 66-67 -2.783164954393733 2117 68-69 -1.0804074548655223 2117 70-71 0.9492354370155525 2117 72-73 0.9483973233535252 2117 74-75 -0.42164868519598997 2117 76 -1.1352874851787185 2118 1 0.5854015813229445 2118 2 0.5829863159305368 2118 3 -0.2991002796124391 2118 4 -0.10910418708204617 2118 5 0.37058022710531446 2118 6 0.9237355600841823 2118 7 0.9359613484153186 2118 8 -4.3389565674370765 2118 9 -1.6053042765415846 2118 10-11 -1.4610991349713913 2118 12-13 0.30762784873944327 2118 14-15 -1.0263101832877055 2118 16-17 -0.7351189775828146 2118 18-19 -0.5926859258493593 2118 20-21 -2.613509284024765 2118 22-23 -0.5338466331032663 2118 24-25 -0.3071318982393336 2118 26-27 1.0946820782627427 2118 28-29 0.17547520444961862 2118 30-31 -0.6368848185307598 2118 32-33 -0.5293226806250999 2118 34-35 0.5015429800655582 2118 36-37 -0.4704807686393835 2118 38-39 -0.2914812764335508 2118 40-41 -1.6907240747660417 2118 42-43 -1.134237209490152 2118 44-45 0.39686930455962255 2118 46-47 -0.8767183964149012 2118 48-49 -1.0255764417198634 2118 50-51 0.26559338785627773 2118 52-53 -2.1766049540510792 2118 54-55 -0.18373713398646885 2118 56-57 1.1887696043925118 2118 58-59 0.3636602761388019 2118 60-61 -1.967585482736915 2118 62-63 0.26786818043783 2118 64-65 -0.5469969508400396 2118 66-67 -2.0155122826380065 2118 68-69 0.8326710279589022 2118 70-71 -1.2089527786333036 2118 72-73 -2.4944700430586906 2118 74-75 0.18000327091088053 2118 76 -0.21460523327032277 2119 1 0.4261781636394666 2119 2 1.1225667691372436 2119 3 0.6089961320002288 2119 4 0.36772456810217946 2119 5 0.20324236571203258 2119 6 0.705667398757015 2119 7 -1.5631671002232608 2119 8 0.6008763351294917 2119 9 0.23679465956468704 2119 10-11 0.0022356008583912512 2119 12-13 0.6046765955086997 2119 14-15 0.10424219995445583 2119 16-17 -0.13455998257168744 2119 18-19 -0.719992531607609 2119 20-21 -0.8115618175344181 2119 22-23 0.9812101680027041 2119 24-25 -0.1256989475871677 2119 26-27 0.3939848371736119 2119 28-29 1.1813055825225902 2119 30-31 0.3428290721947249 2119 32-33 0.22297641175934046 2119 34-35 0.6904592510278675 2119 36-37 -0.6022352916904126 2119 38-39 0.24097649840566504 2119 40-41 0.28231794002027755 2119 42-43 -1.2654808231224166 2119 44-45 -1.1739667836774395 2119 46-47 -0.2250985130224663 2119 48-49 -0.8653612586940795 2119 50-51 0.4300761672035165 2119 52-53 -1.8448444203010048 2119 54-55 -0.4348641400572788 2119 56-57 1.0834020501459563 2119 58-59 1.0878593504911507 2119 60-61 0.018860390918192138 2119 62-63 -0.4014231581448442 2119 64-65 0.5754409886923213 2119 66-67 0.38858701411020746 2119 68-69 -6.522875426853147E-4 2119 70-71 -1.0580541791261808 2119 72-73 -1.9282020860952898 2119 74-75 0.5302767577173952 2119 76 0.8394910581283668 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 11.0 13 60.0 14 191.0 15 402.0 16 528.0 17 631.0 18 660.0 19 626.0 20 583.0 21 562.0 22 535.0 23 595.0 24 598.0 25 740.0 26 849.0 27 1017.0 28 1194.0 29 1451.0 30 1780.0 31 2167.0 32 2791.0 33 3407.0 34 4801.0 35 6930.0 36 10784.0 37 10206.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.1103746183064 22.10099054144634 11.167796231473897 32.62083860877337 2 16.517560073937155 20.236598890942698 39.090573012939004 24.155268022181144 3 17.46765249537893 25.482439926062845 29.868761552680223 27.181146025878007 4 12.425138632162662 18.578558225508317 40.380776340110906 28.615526802218117 5 11.587800369685768 36.15526802218115 38.09981515711645 14.157116451016636 6 25.64510166358595 40.5452865064695 18.299445471349355 15.510166358595196 7 24.1275415896488 33.13678373382625 23.273567467652494 19.46210720887246 8 20.90757855822551 35.42329020332717 19.41589648798521 24.25323475046211 9 23.166358595194083 16.01109057301294 22.21996303142329 38.60258780036968 10-11 23.564695009242147 26.233826247689464 27.289279112754162 22.912199630314234 12-13 22.815157116451015 23.78743068391867 26.588724584103513 26.8086876155268 14-15 21.271719038817004 25.363216266173755 23.922365988909426 29.44269870609981 16-17 22.109981515711645 27.504621072088725 25.811460258780038 24.573937153419593 18-19 21.140480591497226 26.89001848428835 26.64695009242144 25.322550831792977 20-21 20.644177449168208 27.070240295748615 26.75785582255083 25.52772643253235 22-23 22.34011090573013 26.887245841035117 26.29482439926063 24.477818853974124 24-25 21.551756007393717 28.23844731977819 26.111829944547132 24.097966728280962 26-27 20.179297597042513 28.317005545286506 27.548059149722736 23.955637707948245 28-29 21.470425138632162 27.15619223659889 28.31977818853974 23.053604436229204 30-31 21.69870609981516 26.719038817005547 27.951940850277264 23.630314232902034 32-33 20.230129390018483 28.271719038817007 27.510166358595196 23.987985212569317 34-35 21.869685767097966 27.759704251386317 27.040665434380777 23.329944547134936 36-37 21.859519408502774 27.150646950092423 27.143253234750464 23.846580406654343 38-39 21.551756007393717 27.24029574861368 27.72365988909427 23.484288354898336 40-41 21.691312384473196 26.44547134935305 27.795748613678374 24.067467652495377 42-43 21.288354898336415 26.32347504621072 27.976894639556377 24.411275415896487 44-45 20.422365988909426 26.55730129390019 28.09704251386322 24.923290203327173 46-47 21.015711645101664 26.408502772643256 28.728280961182996 23.847504621072087 48-49 19.952865064695008 26.747689463955638 29.02125693160813 24.27818853974122 50-51 20.015711645101664 26.43992606284658 28.73567467652495 24.808687615526804 52-53 19.902033271719038 26.720887245841034 28.77541589648798 24.60166358595194 54-55 18.971349353049906 27.13031423290203 28.482439926062845 25.41589648798521 56-57 19.937153419593344 26.707948243992607 28.22550831792976 25.129390018484287 58-59 19.573937153419593 26.997227356746766 27.89279112754159 25.536044362292053 60-61 19.523105360443623 26.50739371534196 28.138632162661736 25.83086876155268 62-63 19.629390018484287 26.318853974121996 28.26894639556377 25.782809611829943 64-65 20.27264325323475 27.110905730129392 27.170055452865068 25.446395563770796 66-67 20.54898336414048 27.15249537892791 26.322550831792974 25.975970425138634 68-69 19.70332717190388 27.095194085027725 26.670055452865064 26.53142329020333 70-71 21.05822550831793 26.486136783733826 26.149722735674676 26.30591497227357 72-73 20.628465804066543 26.106284658040664 26.498151571164506 26.767097966728283 74-75 20.819778188539743 26.279112754158966 26.498151571164506 26.402957486136785 76 21.545286506469502 24.972273567467653 26.826247689463955 26.65619223659889 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.0 9 0.5 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 1.0 16 1.5 17 1.0 18 1.0 19 9.0 20 15.5 21 14.0 22 15.5 23 27.0 24 43.5 25 50.0 26 83.5 27 132.0 28 204.5 29 262.0 30 342.0 31 444.0 32 646.0 33 826.0 34 942.5 35 1239.5 36 1521.5 37 1623.0 38 1847.0 39 2386.0 40 2966.0 41 3327.5 42 3424.0 43 3537.0 44 3800.5 45 4010.0 46 4069.0 47 4134.0 48 3987.5 49 3532.5 50 3289.0 51 3110.0 52 2641.0 53 2167.0 54 1983.0 55 1842.0 56 1498.5 57 1122.5 58 949.0 59 805.5 60 549.5 61 375.5 62 314.0 63 247.0 64 159.0 65 110.0 66 71.5 67 61.0 68 48.5 69 32.5 70 31.5 71 34.0 72 23.0 73 13.0 74 12.5 75 11.0 76 7.5 77 4.0 78 4.0 79 4.0 80 3.0 81 2.0 82 2.0 83 2.0 84 2.0 85 1.5 86 1.0 87 1.0 88 1.0 89 1.5 90 2.0 91 2.0 92 2.0 93 1.0 94 0.0 95 0.5 96 1.0 97 0.5 98 0.5 99 2.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7245841035120147 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 54100.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.645101663585955 #Duplication Level Percentage of deduplicated Percentage of total 1 77.49025487256371 47.76894639556377 2 10.194902548725636 12.56931608133087 3 4.494752623688155 8.312384473197781 4 2.575712143928036 6.351201478743068 5 1.5142428785607196 4.66728280961183 6 1.103448275862069 4.081330868761553 7 0.6986506746626686 3.014787430683919 8 0.5007496251874063 2.4695009242144175 9 0.3448275862068966 1.9131238447319776 >10 1.0824587706146926 8.852125693160813 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.005545286506469501 0.0 2 0.0 0.0 0.0 0.018484288354898338 0.0 3 0.0 0.0 0.0 0.027726432532347505 0.0 4 0.0 0.0 0.0 0.033271719038817 0.0 5 0.0 0.0 0.0 0.033271719038817 0.0 6 0.0 0.0 0.0 0.033271719038817 0.0 7 0.0 0.0 0.0 0.03512014787430684 0.0 8 0.0 0.0 0.0 0.03512014787430684 0.0 9 0.0 0.0 0.0 0.06839186691312385 0.0 10 0.0 0.0 0.0 0.08872458410351201 0.0 11 0.0 0.0 0.0 0.09057301293900184 0.0 12 0.0 0.0 0.0 0.09796672828096119 0.0 13 0.0 0.0 0.0 0.10166358595194085 0.0 14 0.0 0.0 0.0 0.10905730129390019 0.0 15 0.0 0.0 0.0 0.12199630314232902 0.0 16 0.0 0.0 0.0 0.12939001848428835 0.0 17 0.0 0.0 0.0 0.12939001848428835 0.0 18 0.0 0.0 0.0 0.1367837338262477 0.0 19 0.0 0.0 0.0 0.14417744916820702 0.0 20 0.0 0.0 0.0 0.1478743068391867 0.0 21 0.0 0.0 0.0 0.1534195933456562 0.0 22 0.0 0.0 0.0 0.16635859519408502 0.0 23 0.0 0.0 0.0 0.17190388170055454 0.0 24 0.0 0.0 0.0 0.18114602587800369 0.0 25 0.0 0.0 0.0 0.18853974121996303 0.0 26 0.0 0.0 0.0 0.19963031423290203 0.0 27 0.0 0.0 0.0 0.22181146025878004 0.0 28 0.0 0.0 0.0 0.24584103512014788 0.0 29 0.0 0.0 0.0 0.27171903881700554 0.0 30 0.0 0.0 0.0 0.29390018484288355 0.0 31 0.0 0.0 0.0 0.3364140480591497 0.0 32 0.0 0.0 0.0 0.37153419593345655 0.0 33 0.0 0.0 0.0 0.40850277264325324 0.0 34 0.0 0.0 0.0 0.4510166358595194 0.0 35 0.0 0.0 0.0 0.5526802218114603 0.0 36 0.0 0.0 0.0 0.6709796672828097 0.0 37 0.0 0.0 0.0 0.8817005545286506 0.0 38 0.0 0.0 0.0 1.2070240295748613 0.0 39 0.0 0.0 0.0 1.567467652495379 0.0 40 0.0 0.0 0.0 1.9482439926062847 0.0 41 0.0 0.0 0.0 2.5415896487985212 0.0 42 0.0 0.0 0.0 3.133086876155268 0.0 43 0.0 0.0 0.0 3.7356746765249538 0.0 44 0.0 0.0 0.0 4.367837338262477 0.0 45 0.0 0.0 0.0 5.0258780036968576 0.0 46 0.0 0.0 0.0 5.656192236598891 0.0 47 0.0 0.0 0.0 6.3012939001848425 0.0 48 0.0 0.0 0.0 6.88539741219963 0.0 49 0.0 0.0 0.0 7.491682070240295 0.0 50 0.0 0.0 0.0 8.040665434380776 0.0 51 0.0 0.0 0.0 8.689463955637708 0.0 52 0.0 0.0 0.0 9.308687615526802 0.0 53 0.0 0.0 0.0 9.89463955637708 0.0 54 0.0 0.0 0.0 10.5637707948244 0.0 55 0.0 0.0 0.0 11.22920517560074 0.0 56 0.0 0.0 0.0 11.789279112754159 0.0 57 0.0 0.0 0.0 12.353049907578558 0.0 58 0.0 0.0 0.0 12.87985212569316 0.0 59 0.0 0.0 0.0 13.34380776340111 0.0 60 0.0 0.0 0.0 13.807763401109057 0.0 61 0.0 0.0 0.0 14.295748613678374 0.0 62 0.0 0.0 0.0 14.833641404805915 0.0 63 0.0 0.0 0.0 15.312384473197781 0.0 64 0.0 0.0 0.0 15.842883548983364 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAATAGC 15 0.0022143582 69.99261 50 ACTCGCC 15 0.0022143582 69.99261 45 TTCAGTG 20 0.006919351 52.494457 13 CTGAGTC 20 0.006919351 52.494457 69 TTAGAGT 20 0.006919351 52.494457 4 TATCTGA 20 0.006919351 52.494457 55 TGTTCCC 20 0.006919351 52.494457 2 CTCGCCA 20 0.006919351 52.494457 46 TGTAGGG 20 0.006919351 52.494457 4 TAATGGC 20 0.006919351 52.494457 26 CCCAACT 30 5.832627E-4 46.661736 23 TAGTTGT 35 0.0012458405 39.995773 23 TCACTTT 35 0.0012458405 39.995773 27 GTTATGT 40 0.0023144523 35.256985 1 AATAGCC 40 0.0024004248 34.996304 51 CGGAGGC 40 0.0024004248 34.996304 27 ATAGCCC 45 0.004274825 31.107824 52 TGTAAGG 45 0.004274825 31.107824 2 CAATACT 45 0.004274825 31.107824 4 ACGAAAG 45 0.004274825 31.107824 37 >>END_MODULE