FastQCFastQC Report
Wed 25 May 2016
SRR1780636_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780636_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54100
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT7391.365988909426987No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT3420.6321626617375231Illumina PCR Primer Index 9 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC1020.18853974121996303No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC930.1719038817005545No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG670.12384473197781884No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAATCG207.860631E-570.0000148
CTGTCGC207.860631E-570.0000155
TTATCAT207.860631E-570.0000110
GCTGTCG207.860631E-570.0000154
GACTATC207.860631E-570.0000138
GTTAGCA150.002213430870.051
TAGCCAC150.002213430870.028
ATCGTAT150.002213430870.039
TTATAGT150.002213430870.018
ATATCGT150.002213430870.037
TATCGTA150.002213430870.038
ACAATTC252.3706579E-455.99999621
CACAATT252.3706579E-455.99999620
TGTCGCC252.3706579E-455.99999656
GGGTGTC200.006916460652.50000415
GTTCGGG200.006916460652.50000437
CTTTGGG200.006916460652.50000421
TCCAAGC200.006916460652.50000467
ACGACAA200.006916460652.50000450
AAATCGC305.8295915E-446.66666449
Produced by FastQC (version 0.11.5)