##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780636_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 54100 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.78761552680222 34.0 34.0 34.0 31.0 34.0 2 33.306358595194084 34.0 34.0 34.0 32.0 34.0 3 33.49646950092421 34.0 34.0 34.0 34.0 34.0 4 33.46426987060998 34.0 34.0 34.0 34.0 34.0 5 33.481959334565616 34.0 34.0 34.0 34.0 34.0 6 37.19820702402958 38.0 37.0 38.0 36.0 38.0 7 36.80260628465804 38.0 37.0 38.0 35.0 38.0 8 36.86426987060998 38.0 37.0 38.0 35.0 38.0 9 36.686876155268024 38.0 37.0 38.0 34.0 38.0 10-11 36.4107763401109 38.0 37.0 38.0 34.0 38.0 12-13 36.2639741219963 38.0 37.0 38.0 34.0 38.0 14-15 36.53520332717191 38.0 37.0 38.0 34.0 38.0 16-17 36.69535120147874 38.0 37.0 38.0 34.0 38.0 18-19 36.68463955637708 38.0 37.0 38.0 34.0 38.0 20-21 36.41586876155268 38.0 37.0 38.0 34.0 38.0 22-23 36.49678373382625 38.0 37.0 38.0 34.0 38.0 24-25 35.852560073937155 38.0 36.5 38.0 32.5 38.0 26-27 36.04297597042513 38.0 37.0 38.0 33.0 38.0 28-29 35.92814232902033 38.0 37.0 38.0 32.5 38.0 30-31 35.64766173752311 38.0 36.5 38.0 31.5 38.0 32-33 36.12228280961183 38.0 37.0 38.0 34.0 38.0 34-35 36.18286506469501 38.0 37.0 38.0 33.0 38.0 36-37 36.3080314232902 38.0 37.0 38.0 34.0 38.0 38-39 35.911136783733824 38.0 36.5 38.0 32.5 38.0 40-41 35.505526802218114 38.0 36.0 38.0 31.0 38.0 42-43 35.90564695009242 38.0 36.5 38.0 32.5 38.0 44-45 36.08967652495379 38.0 37.0 38.0 33.5 38.0 46-47 36.389436229205174 38.0 37.0 38.0 34.0 38.0 48-49 36.191719038817006 38.0 37.0 38.0 34.0 38.0 50-51 36.328308687615525 38.0 37.0 38.0 34.0 38.0 52-53 36.22829020332717 38.0 37.0 38.0 34.0 38.0 54-55 36.40644177449168 38.0 37.0 38.0 34.0 38.0 56-57 36.436487985212565 38.0 37.0 38.0 34.0 38.0 58-59 36.4914787430684 38.0 37.0 38.0 34.0 38.0 60-61 36.22753234750462 38.0 37.0 38.0 33.5 38.0 62-63 36.471173752310534 38.0 37.0 38.0 34.0 38.0 64-65 36.45776340110906 38.0 37.0 38.0 34.0 38.0 66-67 35.86268022181146 38.0 37.0 38.0 31.5 38.0 68-69 35.29072088724584 38.0 36.0 38.0 29.0 38.0 70-71 35.237661737523105 38.0 36.0 38.0 29.0 38.0 72-73 34.963604436229204 38.0 36.0 38.0 26.0 38.0 74-75 35.565859519408505 38.0 36.5 38.0 31.0 38.0 76 35.05604436229205 38.0 36.0 38.0 27.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8263792279641393 1101 2 0.4952839070579955 1101 3 0.2875073479441923 1101 4 0.46084296122153035 1101 5 0.31043151649718936 1101 6 -1.092501030804236 1101 7 0.33110817292356387 1101 8 0.4860788817594752 1101 9 0.3931090457598785 1101 10-11 0.5745688732011658 1101 12-13 0.7777161389093266 1101 14-15 0.8621579770483692 1101 16-17 0.3546864076943521 1101 18-19 -0.2739907129963228 1101 20-21 0.012029003705549712 1101 22-23 0.591211861040037 1101 24-25 -1.6328398213411859 1101 26-27 -0.1083403782901371 1101 28-29 0.7532784642127766 1101 30-31 1.0290004852315846 1101 32-33 0.54383212905271 1101 34-35 0.4560558812722846 1101 36-37 0.031281809849240005 1101 38-39 0.6849935776776519 1101 40-41 1.156142768444731 1101 42-43 0.8876990966456617 1101 44-45 -0.0016758082632719606 1101 46-47 0.7620736899330822 1101 48-49 -0.5050522986678772 1101 50-51 -0.8293736691330764 1101 52-53 -1.2202268641279943 1101 54-55 -0.48951609977054034 1101 56-57 -1.168743504306157 1101 58-59 -0.6270102061938445 1101 60-61 -1.464950550208222 1101 62-63 -0.8542423623980753 1101 64-65 -0.7705062914205669 1101 66-67 -1.2939279034431976 1101 68-69 -0.6748519378123561 1101 70-71 -1.0085249531787284 1101 72-73 -1.7222272582996254 1101 74-75 -1.1715287889260395 1101 76 -1.3021146647254795 1102 1 -0.6373062814964356 1102 2 -0.02387228050810819 1102 3 0.09794335687590916 1102 4 0.054097162655303066 1102 5 0.25478455445699666 1102 6 0.17621444692522203 1102 7 -0.012116647854440998 1102 8 0.49201377422627246 1102 9 0.3819443965649114 1102 10-11 0.727524567172253 1102 12-13 0.47051200211299005 1102 14-15 -0.225602041755252 1102 16-17 -0.002259179331154826 1102 18-19 -0.3102758228799729 1102 20-21 0.7584739443566235 1102 22-23 0.518259692747641 1102 24-25 -4.560857214689406 1102 26-27 -1.2974048982102175 1102 28-29 -0.16210524715165775 1102 30-31 1.4386549687196535 1102 32-33 -0.3145237294500518 1102 34-35 0.7887918079381677 1102 36-37 -2.8473467011391236 1102 38-39 -0.695779721182376 1102 40-41 0.7755751341751349 1102 42-43 0.48791651349841203 1102 44-45 0.6622976316239075 1102 46-47 1.2125672849501257 1102 48-49 -2.50043953571101 1102 50-51 -0.5468199025330023 1102 52-53 0.30586315820130494 1102 54-55 0.2948298868318844 1102 56-57 0.5260208628345211 1102 58-59 0.5580291638848962 1102 60-61 -6.384623837315992 1102 62-63 -2.8150191869367944 1102 64-65 -0.12739311713451684 1102 66-67 0.9434677952287984 1102 68-69 -1.4268792030609205 1102 70-71 -1.57933233359946 1102 72-73 -5.938968355960931 1102 74-75 -2.819342868136289 1102 76 -1.0600768224408341 1103 1 0.48744095008758137 1103 2 0.4290405745713315 1103 3 -0.10350103407257194 1103 4 0.41558007069574643 1103 5 0.2663878284098118 1103 6 0.24963765347312972 1103 7 0.4667952442977068 1103 8 0.5195053486124124 1103 9 0.19321064596307735 1103 10-11 0.37124146654635126 1103 12-13 -0.6426801018831512 1103 14-15 -0.0026121524918920613 1103 16-17 0.0880874744964828 1103 18-19 0.5263394876640817 1103 20-21 -0.7583291370107332 1103 22-23 0.2486159758482671 1103 24-25 -2.9839193235001957 1103 26-27 -0.2298796213686174 1103 28-29 0.01685479136543222 1103 30-31 0.5101282474871027 1103 32-33 0.8433241280367128 1103 34-35 0.3131297954201102 1103 36-37 -1.0490077507298778 1103 38-39 -0.10271279773509434 1103 40-41 -0.5598846636101271 1103 42-43 -0.4883232553990453 1103 44-45 1.181483958056262 1103 46-47 0.8553934765726581 1103 48-49 -2.5665204216041246 1103 50-51 -0.22002645043863822 1103 52-53 0.5796715356688082 1103 54-55 0.03570615067395977 1103 56-57 0.5402481136770874 1103 58-59 -0.1903579328892988 1103 60-61 -3.3839497882066993 1103 62-63 -0.9702189943513346 1103 64-65 0.16954958869118997 1103 66-67 -0.018159551906492766 1103 68-69 0.6665247449410074 1103 70-71 0.6627475893663544 1103 72-73 -3.981409376663869 1103 74-75 -1.8117116692918032 1103 76 -3.342551545599239 1104 1 0.007069876927133123 1104 2 0.22942008121270163 1104 3 0.45703027522533546 1104 4 -0.12188566828801584 1104 5 -0.1749783954991031 1104 6 -0.12312220315769196 1104 7 0.326630791419781 1104 8 0.48484374203429326 1104 9 0.851654668733481 1104 10-11 -0.6290747889881203 1104 12-13 0.646250954410327 1104 14-15 0.4674279065424827 1104 16-17 -0.07164265411410753 1104 18-19 0.17706687539337196 1104 20-21 0.09010012716805704 1104 22-23 -0.08868109351711695 1104 24-25 0.29086456855124965 1104 26-27 0.7878306885618045 1104 28-29 0.828724915759274 1104 30-31 0.9097065734411416 1104 32-33 0.1890125109167471 1104 34-35 0.1159550115280652 1104 36-37 0.825733973916968 1104 38-39 1.1428701666334504 1104 40-41 1.4676295678889204 1104 42-43 0.3855118700489868 1104 44-45 0.8309884910522527 1104 46-47 0.7238100905300655 1104 48-49 -0.06567707351219809 1104 50-51 0.25551645489554176 1104 52-53 -0.23106006880094299 1104 54-55 -0.263408333829517 1104 56-57 -0.07438397571781508 1104 58-59 -0.8962192021281794 1104 60-61 0.41446893412094 1104 62-63 0.38913162761788556 1104 64-65 0.5633931476201397 1104 66-67 -0.1374511395092739 1104 68-69 1.4207941946563736 1104 70-71 1.301752438617882 1104 72-73 0.704359124284899 1104 74-75 -0.06880633511512002 1104 76 -0.6071941768452831 1105 1 0.8170780468617735 1105 2 0.09375831650681477 1105 3 0.15335380463710635 1105 4 -0.13704089704619093 1105 5 -0.4628460425579277 1105 6 0.053266685731195196 1105 7 0.6956258894589951 1105 8 -0.9101317481617812 1105 9 0.5159929040275983 1105 10-11 0.5829350149334473 1105 12-13 -0.1959059083347725 1105 14-15 0.12057333137907733 1105 16-17 0.004317313206158246 1105 18-19 0.4613193263737614 1105 20-21 0.24620715331184329 1105 22-23 0.28621678229988134 1105 24-25 0.14511293456432384 1105 26-27 0.44340666895396197 1105 28-29 0.8806652752363959 1105 30-31 1.0923863120032422 1105 32-33 0.8231628377141291 1105 34-35 -0.6248742368379538 1105 36-37 0.5301253137862503 1105 38-39 -0.0085595719286502 1105 40-41 0.19777662671244656 1105 42-43 0.7476646478267597 1105 44-45 0.7443871838627132 1105 46-47 0.8121475088307193 1105 48-49 -0.2060365506363837 1105 50-51 0.6040662914968493 1105 52-53 0.1912642776042901 1105 54-55 0.49921421519008646 1105 56-57 0.27522795238676423 1105 58-59 0.3767791638848976 1105 60-61 1.077642953728784 1105 62-63 -0.3449165749965033 1105 64-65 0.4561423306266761 1105 66-67 0.4545218997064069 1105 68-69 -0.06321315828480323 1105 70-71 0.8726266216244198 1105 72-73 1.2354253007554874 1105 74-75 0.27790526619207157 1105 76 0.908859744723344 1106 1 0.1309329419666696 1106 2 0.33637195287045074 1106 3 0.45990974869305035 1106 4 0.06317763442234536 1106 5 0.349085775623891 1106 6 0.4021303220948269 1106 7 -1.2232115231284197 1106 8 0.23405531477527575 1106 9 -0.5099249281402365 1106 10-11 0.22585459535302732 1106 12-13 0.9988493364204771 1106 14-15 -0.07606128400553303 1106 16-17 0.20954021530406663 1106 18-19 0.28065936133879177 1106 20-21 0.16375523023492633 1106 22-23 0.35603758649568107 1106 24-25 -0.4017402122888214 1106 26-27 0.8068900605623526 1106 28-29 0.46997908642521224 1106 30-31 -0.20446683484991013 1106 32-33 0.19186913142042528 1106 34-35 0.7011965673578473 1106 36-37 0.7972363277722607 1106 38-39 0.3619386798196089 1106 40-41 1.2880738295096492 1106 42-43 0.7472057317428451 1106 44-45 0.6552263447018731 1106 46-47 0.8113826486908593 1106 48-49 1.1836487640489395 1106 50-51 -0.4119957714402176 1106 52-53 0.3331567601217671 1106 54-55 -0.010357463131590805 1106 56-57 0.35680574958956157 1106 58-59 0.603483709339443 1106 60-61 0.5807242474350787 1106 62-63 0.6898080753531559 1106 64-65 -0.5259162358068892 1106 66-67 0.6076468997064097 1106 68-69 1.5715551983585598 1106 70-71 0.59705843980624 1106 72-73 0.5468107902659725 1106 74-75 -0.028585118423315237 1106 76 0.41309925521285606 1107 1 0.6282331101529124 1107 2 0.22532477220301672 1107 3 0.2310436780548244 1107 4 0.24056986244747947 1107 5 0.2144482612395393 1107 6 0.27842491357929333 1107 7 0.6260847502184888 1107 8 0.050865087281252386 1107 9 0.06907834706557026 1107 10-11 0.40492868581952024 1107 12-13 0.17181561065257256 1107 14-15 0.010842318720854394 1107 16-17 0.7536448448517277 1107 18-19 0.7456943263737585 1107 20-21 0.6026549381219724 1107 22-23 -0.11271517972543421 1107 24-25 0.8590369851972355 1107 26-27 -0.030920862691608875 1107 28-29 0.9562586296667774 1107 30-31 0.3885888436488045 1107 32-33 0.5880362554356466 1107 34-35 0.4571273454405329 1107 36-37 0.7800857568242208 1107 38-39 -0.016391850409654296 1107 40-41 -0.607761347971099 1107 42-43 0.3278466098520809 1107 44-45 0.8867922471538492 1107 46-47 0.6145604835142606 1107 48-49 0.5968115506294396 1107 50-51 0.5306881269398858 1107 52-53 0.5531709231739086 1107 54-55 0.5318882658229995 1107 56-57 0.6893497878297978 1107 58-59 0.5358772651507167 1107 60-61 0.9673977005642271 1107 62-63 0.7008508300667984 1107 64-65 0.6354540394874348 1107 66-67 -0.2626062648505467 1107 68-69 0.7876966518417774 1107 70-71 -0.6008702138186166 1107 72-73 0.6965408070846024 1107 74-75 -0.8908051768459089 1107 76 0.3076730358625852 1108 1 -0.27397587470684925 1108 2 -0.09084135669579751 1108 3 -0.11813312346747296 1108 4 0.381382305568188 1108 5 0.1844987286838986 1108 6 -0.28652089596815244 1108 7 0.07172883063546465 1108 8 -0.9399928592728983 1108 9 -0.8999793181946245 1108 10-11 0.2532781521883507 1108 12-13 1.0547476864364782 1108 14-15 0.6929181026209079 1108 16-17 -0.055302784833052954 1108 18-19 0.22608648323650726 1108 20-21 0.7796426108281906 1108 22-23 -0.036393511817763624 1108 24-25 0.8823678365251126 1108 26-27 -0.4245876120917913 1108 28-29 -0.7006868489466029 1108 30-31 0.19075232507513107 1108 32-33 0.5713654520932181 1108 34-35 0.648634750090153 1108 36-37 1.1590673072503037 1108 38-39 1.3454845457184135 1108 40-41 1.3990021169085267 1108 42-43 -0.27135087504905187 1108 44-45 0.6316420858235006 1108 46-47 0.7042022473928142 1108 48-49 0.9670026650498897 1108 50-51 0.3829674352876964 1108 52-53 0.3833190161663822 1108 54-55 -0.1915129089929195 1108 56-57 0.3896683118638862 1108 58-59 0.5220814514665975 1108 60-61 1.1726388687614602 1108 62-63 0.9250793400361772 1108 64-65 0.6777722325874649 1108 66-67 0.7220259846737349 1108 68-69 1.407722299231537 1108 70-71 0.2756086477682089 1108 72-73 0.24357481055940866 1108 74-75 -0.28449260962492673 1108 76 1.3274463460305341 1109 1 -1.8859668249330959 1109 2 -0.1653762988777956 1109 3 0.1953409841242859 1109 4 0.2817091029538119 1109 5 0.04372447026258186 1109 6 -0.20218203221752162 1109 7 -0.028572828489721758 1109 8 0.06855414927411374 1109 9 0.6555762373609326 1109 10-11 0.5556914251898561 1109 12-13 0.6290940916652303 1109 14-15 0.8758617929175472 1109 16-17 0.6600064157702619 1109 18-19 0.5854379161173497 1109 20-21 0.8272167686964593 1109 22-23 0.37876485922296155 1109 24-25 -0.5177075782561857 1109 26-27 0.18771756638985693 1109 28-29 -1.0399276734815501 1109 30-31 0.4135401581570832 1109 32-33 0.36931668386797867 1109 34-35 -0.5741530829917991 1109 36-37 0.8665836471195831 1109 38-39 0.8024981203790489 1109 40-41 0.1974561138919384 1109 42-43 0.7754691350062544 1109 44-45 0.5040025684781 1109 46-47 0.5978045601127704 1109 48-49 0.5612711416713125 1109 50-51 -0.17918691363135508 1109 52-53 -0.37371969675469074 1109 54-55 -0.8865229642970931 1109 56-57 -0.5269355091516985 1109 58-59 -0.381073400217673 1109 60-61 0.8735564152672453 1109 62-63 0.571509707054787 1109 64-65 0.3225686126779621 1109 66-67 0.017903309962818525 1109 68-69 -0.929318927515574 1109 70-71 -0.32048235273455816 1109 72-73 -0.8112894428342585 1109 74-75 -0.551021015859213 1109 76 -0.12303128091768656 1110 1 -1.0571655837114733 1110 2 -0.3955330847670666 1110 3 0.24196845431863068 1110 4 0.2578237526353391 1110 5 0.44173198291977656 1110 6 0.24347369210062197 1110 7 0.46326123977746647 1110 8 0.7698443664878951 1110 9 0.6853002288683641 1110 10-11 -0.032908934111141264 1110 12-13 0.8146036458053558 1110 14-15 0.8323169619523298 1110 16-17 0.7915187463271778 1110 18-19 -0.12373242521859851 1110 20-21 0.8639022170061139 1110 22-23 -0.4354193641969317 1110 24-25 -0.8854603138433177 1110 26-27 0.9057275288265672 1110 28-29 0.372166071414739 1110 30-31 -0.07672196825154032 1110 32-33 0.12427748739566624 1110 34-35 0.4256631835442093 1110 36-37 1.1112757914932487 1110 38-39 0.530572593676446 1110 40-41 -0.35739853252322007 1110 42-43 0.18876735483313212 1110 44-45 0.8797375023340521 1110 46-47 0.5950893878116119 1110 48-49 0.7893456149687097 1110 50-51 -0.2726255874840433 1110 52-53 0.1691106151839037 1110 54-55 -0.1600612625169191 1110 56-57 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-0.7500580603673583 2116 36-37 0.9045738431980155 2116 38-39 -3.8273095719286445 2116 40-41 -1.2529586674052027 2116 42-43 -0.4102397639379447 2116 44-45 0.24071071327448124 2116 46-47 -0.5674480794045778 2116 48-49 0.3946987434812712 2116 50-51 -1.1064116496796217 2116 52-53 -0.3699489576898287 2116 54-55 0.1532583328371473 2116 56-57 -0.20510292996618062 2116 58-59 -1.3390296596445168 2116 60-61 -0.7975408698006277 2116 62-63 0.4990172485329154 2116 64-65 -1.4006591399615544 2116 66-67 -0.9217648649994743 2116 68-69 -0.5865587465200974 2116 70-71 1.396523680447956 2116 72-73 0.369800300755486 2116 74-75 0.05823614854501358 2116 76 -0.3768020199825415 2117 1 0.6264530468617764 2117 2 0.3796958165068176 2117 3 0.09397880463710351 2117 4 0.016084102953811907 2117 5 -0.04722104255792914 2117 6 0.2735791857311938 2117 7 0.6893758894589936 2117 8 0.36643075183821594 2117 9 -1.123069595972403 2117 10-11 -0.16315873506655265 2117 12-13 -0.8279371583347697 2117 14-15 0.16041708137908017 2117 16-17 -1.2902139367938403 2117 18-19 -1.1878994236262415 2117 20-21 0.17276965331184613 2117 22-23 0.39168553229988134 2117 24-25 -1.6181683154356747 2117 26-27 -2.143312081046041 2117 28-29 -1.6302722247636012 2117 30-31 -0.08495743799676347 2117 32-33 -1.793243412285868 2117 34-35 0.6274695131620476 2117 36-37 -0.8026871862137526 2117 38-39 0.4383154280713555 2117 40-41 0.1126203767124494 2117 42-43 -0.05546035217323464 2117 44-45 -0.9431128161372868 2117 46-47 -0.08628999116928071 2117 48-49 -0.21306780063638087 2117 50-51 -0.09905870850315068 2117 52-53 0.28423302760428726 2117 54-55 -0.4414107848099107 2117 56-57 0.2275717023867614 2117 58-59 -0.9849395861151038 2117 60-61 -0.19735704627121464 2117 62-63 -0.4253853249964976 2117 64-65 -0.8969826693733225 2117 66-67 -0.6228218502935903 2117 68-69 0.09381809171519961 2117 70-71 -0.46331087837558016 2117 72-73 0.611987800755486 2117 74-75 1.0396240161920716 2117 76 0.48073474472334254 2118 1 0.060255527777805185 2118 2 0.3183289271938392 2118 3 -0.7818561190270188 2118 4 -0.048443568801914694 2118 5 0.11869021698405646 2118 6 -0.043393619612317025 2118 7 0.609223217703267 2118 8 0.34669077092218714 2118 9 -1.9525662562777484 2118 10-11 -0.8278474278146462 2118 12-13 -0.8604097251286618 2118 14-15 0.15764394206610177 2118 16-17 -2.8757637936640705 2118 18-19 -0.8535423148476156 2118 20-21 -0.3139312054667798 2118 22-23 0.9220707899334641 2118 24-25 1.1038915605185267 2118 26-27 -1.706855735626199 2118 28-29 -0.13128606064145742 2118 30-31 -0.8133388788364613 2118 32-33 -0.46824937602632843 2118 34-35 -0.09918244294482292 2118 36-37 -2.9648412892671914 2118 38-39 -0.12263399940956532 2118 40-41 -0.4185401671806801 2118 42-43 0.41901483866646316 2118 44-45 -2.1392006023968264 2118 46-47 -0.2406912316272951 2118 48-49 -0.21118922239210747 2118 50-51 -0.6887533649916975 2118 52-53 -1.2943988903346408 2118 54-55 -1.2786006703060977 2118 56-57 0.06723654017302039 2118 58-59 -1.1213601490921974 2118 60-61 -1.3885545653551858 2118 62-63 -1.765885086446879 2118 64-65 -0.2610093869305743 2118 66-67 0.3671888844392299 2118 68-69 -1.9072255628649586 2118 70-71 -0.9005126913526809 2118 72-73 1.0992850335799176 2118 74-75 0.5163356097035248 2118 76 0.8797089813645655 2119 1 0.7003949759956356 2119 2 -0.4417239669577526 2119 3 -0.06682336071722972 2119 4 0.10651225256010832 2119 5 0.05058899681215223 2119 6 -0.13974512529242844 2119 7 -0.34605718140714714 2119 8 0.17111825183821594 2119 9 -0.5832689069960253 2119 10-11 -0.7836988433342711 2119 12-13 -0.5214964595158733 2119 14-15 -0.22051918830595696 2119 16-17 -0.14137658443163303 2119 18-19 -0.5574552799254562 2119 20-21 -1.4210418624361836 2119 22-23 -0.13319758777885937 2119 24-25 0.634876714091881 2119 26-27 0.42315568470198883 2119 28-29 0.10367216500017662 2119 30-31 0.930575288381192 2119 32-33 0.5241470896826357 2119 34-35 0.089914148988818 2119 36-37 0.149392046069714 2119 38-39 -1.019730831771163 2119 40-41 -0.3399202236812542 2119 42-43 0.13179358483463943 2119 44-45 0.4589541129965653 2119 46-47 -0.6946192234527473 2119 48-49 -0.5719814325261439 2119 50-51 0.422594834803931 2119 52-53 -0.06668355704137952 2119 54-55 -0.24935861945558457 2119 56-57 -1.3813419295030016 2119 58-59 -0.5325220172174667 2119 60-61 0.23583685136657806 2119 62-63 0.19300173209011717 2119 64-65 0.21768268495738852 2119 66-67 0.40292249025759475 2119 68-69 1.203100818093155 2119 70-71 0.05446717280552349 2119 72-73 0.8132058125665083 2119 74-75 -0.006174458217380163 2119 76 -0.30998868047350925 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 1.0 21 1.0 22 2.0 23 7.0 24 14.0 25 47.0 26 88.0 27 157.0 28 253.0 29 443.0 30 720.0 31 1146.0 32 1925.0 33 2914.0 34 4575.0 35 7228.0 36 12935.0 37 21643.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.48428835489833 23.491682070240298 12.380776340110906 28.64325323475046 2 17.173752310536045 19.621072088724585 39.53234750462107 23.6728280961183 3 16.190388170055453 26.502772643253238 31.7449168207024 25.56192236598891 4 12.600739371534194 20.295748613678374 39.79297597042514 27.310536044362294 5 11.669131238447319 38.06284658040666 37.77079482439926 12.497227356746766 6 26.833641404805913 41.52125693160813 17.55822550831793 14.08687615526802 7 24.855822550831792 30.65064695009242 24.809611829944547 19.683918669131238 8 22.062846580406653 37.438077634011094 20.835489833641404 19.663585951940853 9 23.216266173752313 14.512014787430683 23.184842883548985 39.08687615526802 10-11 24.472273567467653 26.677449168207023 28.111829944547136 20.73844731977819 12-13 23.309611829944547 23.64510166358595 27.164510166358596 25.880776340110906 14-15 20.920517560073936 25.257855822550834 24.082255083179298 29.739371534195936 16-17 22.351201478743068 28.157116451016634 25.914972273567464 23.57670979667283 18-19 22.559149722735675 27.45194085027726 26.657116451016638 23.331792975970426 20-21 20.880776340110906 27.35120147874307 26.22273567467652 25.545286506469502 22-23 21.73105360443623 28.11460258780037 26.906654343807762 23.247689463955638 24-25 22.3724584103512 28.67467652495379 26.409426987060996 22.54343807763401 26-27 21.87800369685767 28.62569316081331 27.08410351201479 22.412199630314234 28-29 22.258780036968577 26.92329020332717 28.127541589648796 22.690388170055453 30-31 21.215341959334566 27.113678373382626 28.82162661737523 22.849353049907577 32-33 20.917744916820702 29.979667282809615 26.65157116451017 22.45101663585952 34-35 21.361367837338264 28.721811460258778 27.36783733826248 22.548983364140483 36-37 22.79112754158965 27.299445471349355 27.037892791127543 22.871534195933457 38-39 21.54898336414048 27.060998151571162 27.944547134935306 23.44547134935305 40-41 20.777264325323475 26.168207024029577 29.49260628465804 23.56192236598891 42-43 22.298521256931608 26.193160813308687 28.763401109057302 22.7449168207024 44-45 20.465804066543438 27.055452865064694 28.83456561922366 23.644177449168208 46-47 21.75138632162662 26.150646950092423 28.675600739371532 23.422365988909426 48-49 20.93992606284658 25.752310536044366 28.8909426987061 24.41682070240296 50-51 20.17097966728281 26.03974121996303 29.25323475046211 24.53604436229205 52-53 19.40573012939002 26.397412199630317 31.144177449168208 23.05268022181146 54-55 19.157116451016638 26.292051756007396 29.99815157116451 24.55268022181146 56-57 20.33271719038817 26.03327171903882 28.77079482439926 24.86321626617375 58-59 19.164510166358596 26.730129390018487 29.648798521256932 24.45656192236599 60-61 20.16543438077634 27.65619223659889 27.926062846580407 24.252310536044362 62-63 18.311460258780038 29.36506469500924 27.521256931608136 24.802218114602585 64-65 19.24214417744917 30.012014787430687 25.98521256931608 24.760628465804064 66-67 19.19408502772643 29.717190388170057 25.9362292051756 25.152495378927913 68-69 19.182070240295747 29.765249537892792 25.768022181146026 25.284658040665438 70-71 19.54343807763401 29.040665434380774 25.68484288354898 25.731053604436227 72-73 19.695933456561924 28.28650646950092 25.81053604436229 26.20702402957486 74-75 19.85489833641405 28.553604436229207 25.655268022181144 25.9362292051756 76 19.985212569316083 28.465804066543438 25.75785582255083 25.79112754158965 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 1.0 16 1.5 17 1.0 18 3.5 19 7.0 20 12.0 21 16.0 22 18.0 23 30.0 24 49.5 25 59.0 26 95.0 27 154.5 28 249.5 29 321.0 30 352.0 31 446.5 32 676.5 33 843.0 34 965.0 35 1294.5 36 1615.0 37 1728.0 38 1978.0 39 2598.5 40 3429.5 41 4002.5 42 4115.0 43 3999.5 44 4013.0 45 4226.0 46 4310.0 47 4276.0 48 4067.5 49 3539.5 50 3186.0 51 2901.5 52 2327.5 53 1882.0 54 1726.0 55 1587.0 56 1223.5 57 821.5 58 644.0 59 530.5 60 321.0 61 189.0 62 153.0 63 116.0 64 54.5 65 22.0 66 12.5 67 11.0 68 6.5 69 2.0 70 1.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.5 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 54100.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.53604436229205 #Duplication Level Percentage of deduplicated Percentage of total 1 60.20337215365896 25.608133086876155 2 15.717886320180774 13.371534195933457 3 8.204415087780289 10.469500924214417 4 4.810533634625413 8.184842883548983 5 3.0071267164957414 6.395563770794824 6 2.0206848600730054 5.157116451016636 7 1.3601599165652702 4.0499075785582255 8 0.9951329741004693 3.386321626617375 9 0.7995828263514687 3.0609981515711646 >10 2.8550321571354074 17.735674676524955 >50 0.013036676516600034 0.3937153419593346 >100 0.008691117677733357 0.8207024029574862 >500 0.004345558838866679 1.365988909426987 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT 739 1.365988909426987 No Hit ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT 342 0.6321626617375231 Illumina PCR Primer Index 9 (95% over 22bp) CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC 102 0.18853974121996303 No Hit ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 93 0.1719038817005545 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG 67 0.12384473197781884 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.011090573012939002 0.0 2 0.0 0.0 0.0 0.024029574861367836 0.0 3 0.0 0.0 0.0 0.033271719038817 0.0 4 0.0 0.0 0.0 0.038817005545286505 0.0 5 0.0 0.0 0.0 0.038817005545286505 0.0 6 0.0 0.0 0.0 0.038817005545286505 0.0 7 0.0 0.0 0.0 0.04251386321626617 0.0 8 0.0 0.0 0.0 0.04251386321626617 0.0 9 0.0 0.0 0.0 0.07208872458410351 0.0 10 0.0 0.0 0.0 0.09242144177449169 0.0 11 0.0 0.0 0.0 0.09611829944547134 0.0 12 0.0 0.0 0.0 0.10536044362292052 0.0 13 0.0 0.0 0.0 0.10905730129390019 0.0 14 0.0 0.0 0.0 0.11460258780036968 0.0 15 0.0 0.0 0.0 0.12754158964879853 0.0 16 0.0 0.0 0.0 0.13493530499075784 0.0 17 0.0 0.0 0.0 0.13493530499075784 0.0 18 0.0 0.0 0.0 0.14417744916820702 0.0 19 0.0 0.0 0.0 0.1534195933456562 0.0 20 0.0 0.0 0.0 0.15711645101663585 0.0 21 0.0 0.0 0.0 0.16635859519408502 0.0 22 0.0 0.0 0.0 0.17744916820702403 0.0 23 0.0 0.0 0.0 0.18484288354898337 0.0 24 0.0 0.0 0.0 0.19408502772643252 0.0 25 0.0 0.0 0.0 0.2033271719038817 0.0 26 0.0 0.0 0.0 0.2088724584103512 0.0 27 0.0 0.0 0.0 0.22735674676524953 0.0 28 0.0 0.0 0.0 0.24953789279112754 0.0 29 0.0 0.0 0.0 0.2754158964879852 0.0 30 0.0 0.0 0.0 0.29944547134935307 0.0 31 0.0 0.0 0.0 0.34750462107208874 0.0 32 0.0 0.0 0.0 0.38817005545286504 0.0 33 0.0 0.0 0.0 0.4288354898336414 0.0 34 0.0 0.0 0.0 0.4805914972273567 0.0 35 0.0 0.0 0.0 0.5767097966728281 0.0 36 0.0 0.0 0.0 0.6931608133086876 0.0 37 0.0 0.0 0.0 0.9242144177449169 0.0 38 0.0 0.0 0.0 1.266173752310536 0.0 39 0.0 0.0 0.0 1.6395563770794825 0.0 40 0.0 0.0 0.0 2.0591497227356745 0.0 41 0.0 0.0 0.0 2.6709796672828094 0.0 42 0.0 0.0 0.0 3.279112754158965 0.0 43 0.0 0.0 0.0 3.911275415896488 0.0 44 0.0 0.0 0.0 4.6044362292051755 0.0 45 0.0 0.0 0.0 5.303142329020333 0.0 46 0.0 0.0 0.0 5.975970425138632 0.0 47 0.0 0.0 0.0 6.691312384473198 0.0 48 0.0 0.0 0.0 7.303142329020333 0.0 49 0.0 0.0 0.0 7.944547134935305 0.0 50 0.0 0.0 0.0 8.55637707948244 0.0 51 0.0 0.0 0.0 9.247689463955638 0.0 52 0.0 0.0 0.0 9.931608133086876 0.0 53 0.0 0.0 0.0 10.578558225508319 0.0 54 0.0 0.0 0.0 11.303142329020332 0.0 55 0.0 0.0 0.0 12.031423290203326 0.0 56 0.0 0.0 0.0 12.611829944547136 0.0 57 0.0 0.0 0.0 13.22550831792976 0.0 58 0.0 0.0 0.0 13.783733826247689 0.0 59 0.0 0.0 0.0 14.280961182994455 0.0 60 0.0 0.0 0.0 14.757855822550832 0.0 61 0.0 0.0 0.0 15.290203327171904 0.0 62 0.0 0.0 0.0 15.878003696857672 0.0 63 0.0 0.0 0.0 16.401109057301294 0.0 64 0.0 0.0 0.0 16.959334565619223 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAAATCG 20 7.860631E-5 70.00001 48 CTGTCGC 20 7.860631E-5 70.00001 55 TTATCAT 20 7.860631E-5 70.00001 10 GCTGTCG 20 7.860631E-5 70.00001 54 GACTATC 20 7.860631E-5 70.00001 38 GTTAGCA 15 0.0022134308 70.0 51 TAGCCAC 15 0.0022134308 70.0 28 ATCGTAT 15 0.0022134308 70.0 39 TTATAGT 15 0.0022134308 70.0 18 ATATCGT 15 0.0022134308 70.0 37 TATCGTA 15 0.0022134308 70.0 38 ACAATTC 25 2.3706579E-4 55.999996 21 CACAATT 25 2.3706579E-4 55.999996 20 TGTCGCC 25 2.3706579E-4 55.999996 56 GGGTGTC 20 0.0069164606 52.500004 15 GTTCGGG 20 0.0069164606 52.500004 37 CTTTGGG 20 0.0069164606 52.500004 21 TCCAAGC 20 0.0069164606 52.500004 67 ACGACAA 20 0.0069164606 52.500004 50 AAATCGC 30 5.8295915E-4 46.666664 49 >>END_MODULE