FastQCFastQC Report
Wed 25 May 2016
SRR1780635_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780635_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486348
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACT207.844025E-444.00906425
GTCGTTT207.883894E-443.96378733
TTAAGTC250.00233600835.2471819
CCCGGAA250.002343107235.2253912
TATTGCG505.9335434E-835.211
CAGAACG401.8358505E-532.95254
AGAACGC401.8358505E-532.95255
TTGCGAT353.1860324E-431.47718616
GTACAAG651.7389539E-830.4678121
TGCGCCA602.902343E-729.37567914
TTGCGCC602.9180956E-729.35751713
AAGGCGT454.0560306E-529.34239823
CGGAACC300.00573589529.3393761
CTATAAG300.00573589529.3393761
CGAAAGA300.00574456229.33031330
ACCCGAT300.005747453329.32729527
GTACAGA454.0874915E-529.3091932
GTACAAT300.005764825729.3091931
GAACGCC454.104739E-529.2911116
TGTTCGT703.5814082E-828.28571510
Produced by FastQC (version 0.11.5)