FastQCFastQC Report
Wed 25 May 2016
SRR1780635_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780635_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486348
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT117422.414320609933628No Hit
ATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTT39140.8047735366445427RNA PCR Primer, Index 14 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC21750.44721063929531946No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG13140.27017691036048264No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC10600.21795093225427062No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTCG207.8570575E-443.99424426
CCGTCTT4750.040.76513744
GCCGTCT5050.038.34344543
TCCGCTC301.2994191E-436.6694110
AAACGAC250.002338714735.2388731
AATCTAT250.002350551535.20263311
AACGACA250.002350551535.2026332
ATGATTC1600.034.3775740
ATTGGCG1800.034.21774321
TGGCGTG1750.032.68143523
TGCCGTC6000.032.272442
ATCGTAC551.3724457E-731.99581125
CATCGTA551.3724457E-731.99581124
GACATCG551.3724457E-731.99581122
ACATCGT551.3724457E-731.99581123
GAGGCGC2000.031.90238613
CGCCATT2000.031.89582417
TGCAACA353.2136458E-431.4309224
CGTTGAG353.2175248E-431.42445830
TCAATGC353.2175248E-431.42445826
Produced by FastQC (version 0.11.5)