FastQCFastQC Report
Wed 25 May 2016
SRR1780634_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780634_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48288
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACT150.002141031770.5768051
TCATTGA150.00221301369.99171425
TACAGGA150.00221301369.9917142
AATGTAT150.00221301369.99171446
AAGTACC200.006915180552.49378254
ATCTCTG200.006915180552.49378258
TCGTTAC200.006915180552.4937823
ACGTTAG200.006915180552.49378270
CCCACGT200.006915180552.49378259
AGACGTT200.006915180552.49378268
ACTAGGT200.006915180552.49378232
TCTACTG200.006915180552.4937822
TTGAACT200.006915180552.49378228
ACATGAG200.006915180552.4937829
CACGTAA200.006915180552.49378261
CCACGTA200.006915180552.49378260
AGTACCC200.006915180552.49378255
TGACTTG200.006915180552.49378228
TGTCCCA200.006915180552.49378240
ATACTGG305.8266753E-446.661146
Produced by FastQC (version 0.11.5)