##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780634_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 48288 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.650430748840293 34.0 31.0 34.0 12.0 34.0 2 30.930728131212724 34.0 32.0 34.0 21.0 34.0 3 31.245878893306827 34.0 33.0 34.0 23.0 34.0 4 31.430852385685885 34.0 34.0 34.0 23.0 34.0 5 31.490867296222664 34.0 34.0 34.0 23.0 34.0 6 34.26575960901259 38.0 36.0 38.0 23.0 38.0 7 33.697895957587804 38.0 34.0 38.0 21.0 38.0 8 32.749855036447975 37.0 32.0 38.0 11.0 38.0 9 33.314570907886015 37.0 34.0 38.0 21.0 38.0 10-11 33.68728255467197 37.0 34.0 38.0 21.5 38.0 12-13 34.039109095427435 38.0 34.5 38.0 22.0 38.0 14-15 33.62154158383035 37.0 34.0 38.0 21.0 38.0 16-17 33.482915009940356 37.0 34.0 38.0 21.0 38.0 18-19 33.43777957256461 37.0 34.0 38.0 21.0 38.0 20-21 33.56544068919814 37.0 34.0 38.0 21.0 38.0 22-23 33.494201457919154 37.0 34.0 38.0 21.0 38.0 24-25 33.890117627567925 38.0 34.0 38.0 21.5 38.0 26-27 33.948434393638166 38.0 34.0 38.0 21.5 38.0 28-29 34.09703238899934 38.0 34.5 38.0 22.5 38.0 30-31 33.91872722001325 38.0 34.0 38.0 21.5 38.0 32-33 33.69657057654076 37.5 34.0 38.0 21.5 38.0 34-35 33.5625 37.0 34.0 38.0 21.0 38.0 36-37 33.581552352551356 37.0 34.0 38.0 21.0 38.0 38-39 34.02785371106693 38.0 34.0 38.0 22.0 38.0 40-41 33.53833250497018 37.5 34.0 38.0 21.0 38.0 42-43 33.87064073889994 37.5 34.0 38.0 21.5 38.0 44-45 33.0027543074884 37.0 33.0 38.0 16.0 38.0 46-47 33.67592362491716 37.0 34.0 38.0 21.0 38.0 48-49 33.42376988071571 37.0 34.0 38.0 21.0 38.0 50-51 33.423417826375086 37.0 34.0 38.0 21.0 38.0 52-53 33.47163891650099 37.0 34.0 38.0 21.0 38.0 54-55 33.14377277998675 37.0 33.5 38.0 21.0 38.0 56-57 33.232573310139166 37.0 33.5 38.0 20.5 38.0 58-59 33.1388336646786 37.0 33.0 38.0 20.5 38.0 60-61 33.1520874751491 37.0 33.0 38.0 20.5 38.0 62-63 33.217062210072896 37.0 33.0 38.0 20.5 38.0 64-65 33.224662442014576 37.0 33.0 38.0 20.0 38.0 66-67 33.13203073227302 37.0 33.0 38.0 20.0 38.0 68-69 32.71965084493041 37.0 31.5 38.0 15.0 38.0 70-71 32.5469785453943 37.0 31.5 38.0 11.0 38.0 72-73 32.649312458581846 37.0 31.5 38.0 15.0 38.0 74-75 32.23318422796554 37.0 31.0 38.0 11.0 38.0 76 31.401259111994698 36.0 28.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -3.4578581244085207 1101 2 -1.7595577739913857 1101 3 -0.6721004029082849 1101 4 -3.2115865183287546 1101 5 -1.684254658048392 1101 6 -3.300429743897613 1101 7 -1.969157816721932 1101 8 -1.8393573375418875 1101 9 -0.5228331000003337 1101 10-11 -1.8593211792556872 1101 12-13 -1.092158040183378 1101 14-15 -0.34419433661442156 1101 16-17 -1.5599169880065311 1101 18-19 -0.10190855225142315 1101 20-21 -1.5109724176533348 1101 22-23 -0.8577260756123479 1101 24-25 -1.8715783784982527 1101 26-27 -1.7142131280146842 1101 28-29 -1.249191426694523 1101 30-31 -1.7296632573421036 1101 32-33 -0.18219611989690065 1101 34-35 -1.5282611451374564 1101 36-37 -0.42354345561093254 1101 38-39 -1.0458572386595222 1101 40-41 0.38758796608262003 1101 42-43 -1.6492036938645143 1101 44-45 -1.2487327420169194 1101 46-47 -0.025594129967359436 1101 48-49 -1.3808989160633303 1101 50-51 -1.4721722673428168 1101 52-53 -0.5832055771098794 1101 54-55 -1.159919638632708 1101 56-57 -1.212926673722393 1101 58-59 -0.3468648330960562 1101 60-61 -0.043443813251677454 1101 62-63 -1.740624609194434 1101 64-65 -1.9508556951010334 1101 66-67 -1.6549159257599797 1101 68-69 0.47367638893778974 1101 70-71 0.09900550507415318 1101 72-73 -0.9691622617614399 1101 74-75 -1.5456739282752068 1101 76 -3.1378469412362797 1102 1 1.2436618473129357 1102 2 0.331216351141169 1102 3 0.7290307388379169 1102 4 0.9657906467472479 1102 5 1.0854166038816189 1102 6 2.1245967672370654 1102 7 2.094893084656647 1102 8 3.0908653559893935 1102 9 1.881550074266194 1102 10-11 0.9491270356612418 1102 12-13 -3.2751909139903788 1102 14-15 0.43236982033149474 1102 16-17 -0.6839537501132824 1102 18-19 0.5008132575753734 1102 20-21 1.2622336622335553 1102 22-23 0.024493790064575194 1102 24-25 1.6126209852345141 1102 26-27 -4.812693156293225 1102 28-29 0.3534243385935696 1102 30-31 1.238311291968607 1102 32-33 -5.57547996577884 1102 34-35 0.6500350726073307 1102 36-37 1.5427343810804715 1102 38-39 1.696136398668152 1102 40-41 1.414311189836603 1102 42-43 1.4055506362874866 1102 44-45 -5.268527722575417 1102 46-47 0.3010583974274823 1102 48-49 1.3954178036256053 1102 50-51 -6.019644872503655 1102 52-53 -0.46468313101585323 1102 54-55 1.0029647728200999 1102 56-57 -0.968175878388351 1102 58-59 1.5422125794172032 1102 60-61 1.7479312309335455 1102 62-63 -2.501847656207513 1102 64-65 0.2773875003744024 1102 66-67 0.9946068738158331 1102 68-69 -6.898009719227645 1102 70-71 0.7450641830522358 1102 72-73 0.8951007286945512 1102 74-75 -5.344860920145127 1102 76 -0.7547433710701448 1103 1 -0.6572826768381859 1103 2 0.2390355763922436 1103 3 0.5688203132042489 1103 4 1.030293277067667 1103 5 1.02444099412552 1103 6 -0.5082941940254884 1103 7 1.471507144914895 1103 8 2.8304636343251204 1103 9 1.1430876161121972 1103 10-11 1.4896877721509654 1103 12-13 -0.005553180848338002 1103 14-15 0.2425383999584625 1103 16-17 1.4374295593080504 1103 18-19 1.150233391482594 1103 20-21 0.8586887076663601 1103 22-23 0.9527278885820323 1103 24-25 1.3082809565401092 1103 26-27 0.30106820190859196 1103 28-29 1.641025964609824 1103 30-31 0.9838277912512439 1103 32-33 -3.9311411326846297 1103 34-35 0.2976020262180441 1103 36-37 1.0175371070489092 1103 38-39 1.663765523488813 1103 40-41 2.3425751783588424 1103 42-43 1.5823500145753826 1103 44-45 -3.637639391633286 1103 46-47 0.812864949316527 1103 48-49 1.7571765315069996 1103 50-51 1.250142310662298 1103 52-53 1.2170182080563592 1103 54-55 2.0135458345130743 1103 56-57 1.8993815104208238 1103 58-59 1.6903474431187817 1103 60-61 2.20482984403732 1103 62-63 1.18057104297948 1103 64-65 2.0179422588631653 1103 66-67 1.4675265294829885 1103 68-69 -2.8292328182233426 1103 70-71 1.381422384869552 1103 72-73 2.1828518047347174 1103 74-75 -0.3037621635693277 1103 76 1.3258998618327702 1104 1 -2.15852702072959 1104 2 -0.17159790777064998 1104 3 -0.3470167908681496 1104 4 0.2328950870224169 1104 5 -0.08325131356678739 1104 6 -0.3271855967404207 1104 7 1.3963940227429532 1104 8 1.11281657550159 1104 9 0.056096665885952746 1104 10-11 -0.33808372106170737 1104 12-13 -1.1197500134275344 1104 14-15 0.35028726873675 1104 16-17 0.13974856383293854 1104 18-19 0.4755161969124586 1104 20-21 1.094378752915226 1104 22-23 -0.026287948522039528 1104 24-25 0.11721760812381632 1104 26-27 0.2889641295104042 1104 28-29 -0.42628172769786943 1104 30-31 -1.123977638613006 1104 32-33 -0.9731660195624556 1104 34-35 -0.04849525884983308 1104 36-37 -0.5170217164805067 1104 38-39 0.11133339679198428 1104 40-41 0.502303685146174 1104 42-43 0.9436056706839793 1104 44-45 -3.6338497988731007 1104 46-47 0.21688078642060304 1104 48-49 0.7722783414617496 1104 50-51 0.7565902744631998 1104 52-53 0.06696164697039109 1104 54-55 -0.5094179663919007 1104 56-57 0.37319372761540137 1104 58-59 0.4957773073721725 1104 60-61 0.36240902955768206 1104 62-63 0.16422488913332245 1104 64-65 -0.12142425697393833 1104 66-67 0.22284327156443595 1104 68-69 -3.91695906256723 1104 70-71 -0.9053423210128031 1104 72-73 0.10157352419172838 1104 74-75 -1.291827774429052 1104 76 0.45044737314498917 1105 1 -0.973044897219296 1105 2 -0.8075675719093276 1105 3 -0.8603429013773578 1105 4 -0.2326520419049949 1105 5 -0.2713336537618041 1105 6 -0.23747812111745503 1105 7 0.6106952145089295 1105 8 0.4099996740931431 1105 9 0.18047369730523855 1105 10-11 0.2827180124160833 1105 12-13 -1.513682841163174 1105 14-15 -0.3772316911765756 1105 16-17 0.09375723122187907 1105 18-19 0.7996223724270877 1105 20-21 -0.7056754182656988 1105 22-23 0.23985506339561624 1105 24-25 -0.2562385132196283 1105 26-27 -0.5540477881494041 1105 28-29 -0.6881993875028485 1105 30-31 -0.2414207588730264 1105 32-33 -0.632104264416192 1105 34-35 0.0017755970548236633 1105 36-37 0.19002053704062405 1105 38-39 -0.013585346436620682 1105 40-41 -0.10295091940420775 1105 42-43 0.4488061040534248 1105 44-45 1.1229757699243024 1105 46-47 0.8267941125311111 1105 48-49 -0.013691322676926632 1105 50-51 -0.6184368111272605 1105 52-53 -0.5787046585550755 1105 54-55 -0.2964168829682876 1105 56-57 0.05011680453847589 1105 58-59 0.023404609647364794 1105 60-61 0.4690179136313617 1105 62-63 0.4068034373456726 1105 64-65 0.5117285057562881 1105 66-67 0.7795604979999666 1105 68-69 1.589108109697122 1105 70-71 0.9995872564519885 1105 72-73 0.989655864386755 1105 74-75 -0.3393359001061924 1105 76 0.3175112951384911 1106 1 0.6292409490055917 1106 2 0.5623239081183762 1106 3 0.23709418012806793 1106 4 -0.28564211348199464 1106 5 0.327719682650109 1106 6 0.4638610615193528 1106 7 1.3148051198425748 1106 8 0.7128165755015914 1106 9 1.719148368281921 1106 10-11 0.8400373382068906 1106 12-13 -0.11325568808074138 1106 14-15 -0.17398763668569472 1106 16-17 0.6016653355857713 1106 18-19 0.237685175474887 1106 20-21 0.14551368355834882 1106 22-23 0.8273059985145323 1106 24-25 0.8783147077455098 1106 26-27 0.507312174907625 1106 28-29 -0.7790560026032907 1106 30-31 -0.3962348895587837 1106 32-33 0.8960647496683123 1106 34-35 1.0286169731804407 1106 36-37 0.8674045130276937 1106 38-39 0.3940572303354841 1106 40-41 0.2164903181852651 1106 42-43 0.960062165009326 1106 44-45 1.09855877615842 1106 46-47 1.1083688065971486 1106 48-49 0.8275116327606185 1106 50-51 1.2169938053585412 1106 52-53 0.1376426053562696 1106 54-55 0.7788544169624458 1106 56-57 1.5542151651942149 1106 58-59 0.7571266138034503 1106 60-61 1.1977179828994196 1106 62-63 1.1303661249466899 1106 64-65 0.7520309763173572 1106 66-67 1.1501446587018833 1106 68-69 1.546912311959879 1106 70-71 -0.3561619931439495 1106 72-73 0.4848648230568031 1106 74-75 -1.1497722889309436 1106 76 -0.2660721728827511 1107 1 -1.0361189939737372 1107 2 -0.47984762906385114 1107 3 -0.31268011305321153 1107 4 0.4376888439900881 1107 5 -0.30164596239621844 1107 6 -0.707676120709209 1107 7 -0.8937954978813494 1107 8 0.14759918419724016 1107 9 -1.6387751289858379 1107 10-11 -0.6912052372267041 1107 12-13 -0.23418702569063043 1107 14-15 -1.4543392641506543 1107 16-17 -0.2903517706152243 1107 18-19 -0.2490100015267842 1107 20-21 -0.1319869104069653 1107 22-23 -1.7446825973514741 1107 24-25 -1.689694320527238 1107 26-27 -0.92580733091323 1107 28-29 -2.3535392527814807 1107 30-31 -0.9376342718348525 1107 32-33 -0.04958742424473428 1107 34-35 0.3833330577610994 1107 36-37 -1.3604999773500666 1107 38-39 -0.4646978183696646 1107 40-41 -0.30661493246809357 1107 42-43 -0.29920369386451284 1107 44-45 -0.690761727524162 1107 46-47 -0.9770434053296739 1107 48-49 -0.9251018146140524 1107 50-51 -0.11057806444426888 1107 52-53 0.9356350026002644 1107 54-55 -0.8838326821109703 1107 56-57 -0.09843392009920393 1107 58-59 -0.401937296864169 1107 60-61 -1.4992409147009553 1107 62-63 -1.8601898265857386 1107 64-65 -0.3392614922024819 1107 66-67 0.13856233510958305 1107 68-69 -0.36690332120714686 1107 70-71 -0.4017191326070062 1107 72-73 -0.6430753052397016 1107 74-75 -1.315963783347673 1107 76 -1.9450933180478742 1108 1 -0.6890748901263812 1108 2 0.03537497546122381 1108 3 0.16975133309421864 1108 4 -0.6399881448536213 1108 5 0.20789423153559028 1108 6 -0.07859677548972854 1108 7 0.2944017703909907 1108 8 -0.48502515111711375 1108 9 -1.0824202129186915 1108 10-11 -0.5009226806632583 1108 12-13 0.4003662012930036 1108 14-15 -0.07763193436230864 1108 16-17 -1.5942304068366795 1108 18-19 -0.8220211578191439 1108 20-21 -0.2065620329176454 1108 22-23 -1.0301064321966464 1108 24-25 -1.909926830171699 1108 26-27 -1.6123363685526897 1108 28-29 0.06278855453788168 1108 30-31 0.30042745269855686 1108 32-33 0.12987769930860082 1108 34-35 0.3299496775087718 1108 36-37 -0.13176991791934967 1108 38-39 1.0965575251815807 1108 40-41 0.05205465360217687 1108 42-43 0.470971470351941 1108 44-45 -0.19696546019020644 1108 46-47 0.5358071837642697 1108 48-49 -0.14736748034234637 1108 50-51 0.340652255647484 1108 52-53 -0.5383510259681827 1108 54-55 -0.05844951038748292 1108 56-57 0.6562319124521423 1108 58-59 -0.5585945797335299 1108 60-61 0.9494870594414664 1108 62-63 0.10188399261976855 1108 64-65 0.34210645691648267 1108 66-67 0.08014819685497088 1108 68-69 0.8502794543115755 1108 70-71 1.785305161001581 1108 72-73 1.407909993477844 1108 74-75 1.487419596904651 1108 76 2.102190593952958 1109 1 -0.5006922628948303 1109 2 -0.9157574519301939 1109 3 -0.0769313207826805 1109 4 -0.9297829756556446 1109 5 -0.44592937624485174 1109 6 0.4232417536869306 1109 7 -1.3195603932114608 1109 8 0.7831869458719609 1109 9 0.5612248710141614 1109 10-11 0.5358479028699179 1109 12-13 -0.6720292157067291 1109 14-15 0.24872031716979848 1109 16-17 -0.6257785016941284 1109 18-19 0.057994829391091685 1109 20-21 0.45021920875568355 1109 22-23 0.48510806287396235 1109 24-25 0.09798683889305693 1109 26-27 0.20221199275826507 1109 28-29 -0.09878885020498984 1109 30-31 -0.32853604317141105 1109 32-33 0.1479166015201656 1109 34-35 -0.073993834348407 1109 36-37 -0.022577272036059526 1109 38-39 -0.2724272869686999 1109 40-41 0.4114204942629769 1109 42-43 0.44773672481503013 1109 44-45 -0.023450938474248062 1109 46-47 -0.5945152249754031 1109 48-49 0.039229908413318526 1109 50-51 0.24376976164268882 1109 52-53 0.35898443899318266 1109 54-55 0.3518355948616616 1109 56-57 -0.04062393620226601 1109 58-59 -0.6402625786677092 1109 60-61 -0.4704969533482952 1109 62-63 -0.8134104385020038 1109 64-65 -0.48467212022180206 1109 66-67 -0.5921139933928288 1109 68-69 0.7881691425609745 1109 70-71 -0.3460830617535464 1109 72-73 -0.7153780427598342 1109 74-75 0.6024742198729385 1109 76 -1.3355925128948947 1110 1 0.3862175476791023 1110 2 0.4414379113720379 1110 3 -0.35394573978183175 1110 4 0.18257212753915297 1110 5 0.11222725366750552 1110 6 0.6503833991491845 1110 7 1.2961591431187571 1110 8 1.5563280258832677 1110 9 -0.38177767351510283 1110 10-11 -1.039639798571983 1110 12-13 -0.09999076504232107 1110 14-15 0.22095667566569688 1110 16-17 0.26441092437315916 1110 18-19 0.12152324330118347 1110 20-21 -0.7727380996978113 1110 22-23 0.24170970268171743 1110 24-25 0.30867386179381384 1110 26-27 -0.8533435627972921 1110 28-29 0.05419390354111897 1110 30-31 -0.5340774624532898 1110 32-33 1.3197288718057152 1110 34-35 0.2645699202458829 1110 36-37 -0.265494998923252 1110 38-39 0.28643615662756616 1110 40-41 0.3957857755748222 1110 42-43 0.152090697107937 1110 44-45 0.7064614166289545 1110 46-47 0.17178389857561882 1110 48-49 1.137985916329626 1110 50-51 0.595169252736838 1110 52-53 -0.7270489226127026 1110 54-55 -0.3082435682709459 1110 56-57 0.3001168045384759 1110 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52-53 0.5884622774874089 2116 54-55 0.1231167120444141 2116 56-57 0.3288053291286417 2116 58-59 0.20794628593459663 2116 60-61 -0.8309705416907391 2116 62-63 1.0238087478975118 2116 64-65 1.0798998287763766 2116 66-67 -0.13674058719975335 2116 68-69 0.7272401808123448 2116 70-71 -3.2455062554390324 2116 72-73 0.8086353148600836 2116 74-75 -1.1333788463079983 2116 76 -1.2496787302598023 2117 1 0.31683257902025375 2117 2 0.24991553813303824 2117 3 0.5257787063175918 2117 4 0.43733536219439983 2117 5 -1.1015752660370808 2117 6 -1.216866647397083 2117 7 -1.9864077283514838 2117 8 -2.4050696033601966 2117 9 1.1823630823962716 2117 10-11 -0.08745833019241189 2117 12-13 0.9768416063348297 2117 14-15 -0.811532618692894 2117 16-17 -1.167693587511657 2117 18-19 0.00487829822578334 2117 20-21 0.676867808575011 2117 22-23 0.3212417575442501 2117 24-25 0.7670925299499629 2117 26-27 0.8864938355766867 2117 28-29 -2.3419799180146583E-4 2117 30-31 -0.042176512909442465 2117 32-33 0.69687775779839 2117 34-35 0.28418141407074415 2117 36-37 -1.0710867571309137 2117 38-39 0.6839412767169364 2117 40-41 -1.8783717369926691 2117 42-43 -0.8830672498913827 2117 44-45 0.47943975709937803 2117 46-47 -0.365608269239182 2117 48-49 -0.5151513020654548 2117 50-51 0.5819811437565079 2117 52-53 1.2507640234556945 2117 54-55 0.08833062647864409 2117 56-57 0.48710867445717554 2117 58-59 -0.2586004287128745 2117 60-61 0.06093936101484587 2117 62-63 -0.3473761114920677 2117 64-65 -0.474645019950799 2117 66-67 -1.704580118054082 2117 68-69 -0.4020747598780545 2117 70-71 0.562137353783946 2117 72-73 0.08615763926365361 2117 74-75 -0.38144628599878416 2117 76 -0.21002792391567127 2118 1 -0.024261286463854503 2118 2 -0.3714813576541012 2118 3 -0.36076970462106317 2118 4 -0.24577624164891176 2118 5 -0.36949839981387456 2118 6 -0.008071377626201581 2118 7 -0.2040721777232477 2118 8 -3.4159713032862875 2118 9 -1.4039266441373535 2118 10-11 -0.06389957187755613 2118 12-13 0.5861357674582521 2118 14-15 -0.5162628478133655 2118 16-17 -0.571148872064505 2118 18-19 -1.166500120103862 2118 20-21 -2.8123228787864036 2118 22-23 -0.8807168529509468 2118 24-25 0.6828353237415357 2118 26-27 0.1428685584148326 2118 28-29 -0.5370043384204735 2118 30-31 -0.16144850108386777 2118 32-33 0.4869738405774058 2118 34-35 0.8342553239007557 2118 36-37 -0.415506564965348 2118 38-39 0.31494645040503855 2118 40-41 -1.8379061050636203 2118 42-43 -0.45697707989877046 2118 44-45 0.22343458341128297 2118 46-47 -0.8953569758171582 2118 48-49 -0.46396874478533334 2118 50-51 -0.6622908444179174 2118 52-53 -1.103725998717259 2118 54-55 -0.2995112064851426 2118 56-57 0.6902683196899915 2118 58-59 0.7923973539922216 2118 60-61 -1.4859009937523382 2118 62-63 -0.09405016914173103 2118 64-65 -0.5041165646662478 2118 66-67 -2.8560028822817216 2118 68-69 0.18513883953066568 2118 70-71 -0.6288271694976508 2118 72-73 -1.5068763592581504 2118 74-75 0.7634169808156983 2118 76 -0.19081136811374932 2119 1 0.6410279697344912 2119 2 0.9460117552935579 2119 3 0.38991900061309437 2119 4 0.5414138409957161 2119 5 1.0864244651998973 2119 6 1.3789809639715926 2119 7 -0.21371405100148877 2119 8 1.353312443270184 2119 9 0.8019962208764184 2119 10-11 0.4192271498855291 2119 12-13 1.4811082192488882 2119 14-15 -0.5424336467114372 2119 16-17 0.5118401086517537 2119 18-19 0.6974805961495818 2119 20-21 -1.2913173691445294 2119 22-23 1.2384291525586946 2119 24-25 0.6897223760831324 2119 26-27 0.7871523049713005 2119 28-29 0.3672656340313125 2119 30-31 0.4174082482274315 2119 32-33 0.9084614438831835 2119 34-35 1.2770927894654918 2119 36-37 0.5445485314533798 2119 38-39 0.6820263402121967 2119 40-41 0.5277963742752263 2119 42-43 -0.38121950414119965 2119 44-45 -0.1191962705832097 2119 46-47 0.7265438506291204 2119 48-49 0.12211940948463962 2119 50-51 0.986469486160594 2119 52-53 -0.324456026265473 2119 54-55 -0.5109437133721926 2119 56-57 0.8290424243731849 2119 58-59 1.450799557848974 2119 60-61 0.9772532762201109 2119 62-63 0.5126991421530391 2119 64-65 0.416337980788299 2119 66-67 1.312385547470349 2119 68-69 1.0983619800265387 2119 70-71 0.16077338146653375 2119 72-73 -1.8660361388724738 2119 74-75 -0.20352516794462616 2119 76 1.288527474861457 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 20.0 13 98.0 14 239.0 15 449.0 16 488.0 17 596.0 18 588.0 19 553.0 20 533.0 21 432.0 22 451.0 23 443.0 24 442.0 25 514.0 26 626.0 27 681.0 28 827.0 29 1011.0 30 1308.0 31 1646.0 32 2137.0 33 2801.0 34 3913.0 35 5681.0 36 9652.0 37 12158.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.583630561291535 22.90428216840832 11.939177773606158 31.572909496693992 2 17.85122597746852 20.338386348575217 36.15598078197482 25.654406891981445 3 16.66459575878065 26.74163353214049 28.94715043074884 27.64662027833002 4 13.953777335984096 19.443754141815774 36.58465871438039 30.017809807819752 5 12.406809145129225 36.2139662027833 36.29473161033797 15.084493041749504 6 27.770874751491053 37.48136182902584 17.91128230616302 16.83648111332008 7 25.548790589794567 30.947647448641487 22.964297548045064 20.539264413518886 8 22.26433068257124 35.19093770709078 20.866467859509612 21.678263750828364 9 23.125828363154408 17.5240225314778 23.21901921802518 36.13112988734261 10-11 23.896206096752817 26.307778330019882 27.01706428098078 22.77895129224652 12-13 23.406436381709742 24.462599403578526 26.050985752153743 26.079978462557985 14-15 21.617171968190853 25.89049039098741 23.41472001325381 29.077617627567925 16-17 21.541583830351225 27.907554671968192 26.01681577203446 24.534045725646124 18-19 21.573682902584494 27.271785950960904 26.39165009940358 24.762881047051028 20-21 20.674908880053014 27.615556660039765 26.668116302186878 25.041418157720347 22-23 22.199097084161696 27.427104042412193 26.542826375082836 23.83097249834327 24-25 21.38937210072896 28.23165175612989 26.50555003313453 23.873426110006626 26-27 20.432612657388997 28.501905235255137 27.238651424784628 23.82683068257124 28-29 21.509484758117956 27.337019549370446 28.098078197481772 23.055417495029822 30-31 21.004183233929755 27.136141484426773 28.284459907223326 23.575215374420146 32-33 20.47195990722333 28.32691351888668 27.082297879390328 24.11882869449967 34-35 21.72796554009278 27.477841285619615 27.24900596421471 23.545187210072896 36-37 21.7787027833002 27.55239396951624 26.843108018555334 23.82579522862823 38-39 21.26822398939695 27.485089463220675 27.659045725646124 23.58764082173625 40-41 21.288933068257123 26.616343605036448 27.79572564612326 24.298997680583167 42-43 20.856113320079523 26.410288270377734 28.364189860834987 24.369408548707753 44-45 20.083664678595095 26.462060967528167 28.333126242544733 25.12114811133201 46-47 20.055707422133864 26.086191186216034 29.399643803843606 24.458457587806496 48-49 19.84240390987409 26.426855533465872 29.440026507620942 24.2907140490391 50-51 19.777170311464545 26.37508283631544 28.817718687872762 25.03002816434725 52-53 18.693050033134526 27.07090788601723 29.07244035785288 25.16360172299536 54-55 18.328570245195493 27.637301192842944 28.634443339960235 25.399685222001327 56-57 18.827659045725646 26.80686713055003 28.638585155732272 25.72688866799205 58-59 19.027501656726308 26.437210072895954 28.22026176275679 26.315026507620942 60-61 18.84526176275679 26.12036116633532 28.905732273028494 26.128644797879392 62-63 18.776921802518224 26.4061464546057 28.378686216037114 26.438245526838966 64-65 20.234840954274354 26.19180748840292 26.949759774685223 26.623591782637508 66-67 19.794773028495694 26.62669814446653 26.57078363154407 27.007745195493705 68-69 19.760603048376407 26.571819085487075 26.32848740888005 27.33909045725646 70-71 20.58171802518224 25.982645791915175 26.6349817760106 26.800654406891983 72-73 20.88717693836978 25.47734426772697 26.27878561961564 27.356693174287606 74-75 21.05388502319417 25.609882372432075 26.224942014579188 27.111290589794567 76 21.040424121935057 24.519549370444 26.221835652750165 28.218190854870773 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.0 11 1.0 12 1.0 13 0.5 14 0.0 15 3.0 16 6.5 17 7.0 18 6.0 19 4.5 20 10.0 21 16.0 22 23.0 23 32.0 24 40.5 25 47.0 26 72.5 27 111.0 28 177.0 29 230.0 30 272.5 31 389.0 32 568.5 33 674.0 34 798.0 35 1065.0 36 1302.5 37 1397.0 38 1668.0 39 2194.0 40 2543.5 41 2864.5 42 3091.0 43 3207.5 44 3431.5 45 3601.5 46 3664.0 47 3660.5 48 3519.5 49 3260.0 50 3138.0 51 2908.0 52 2441.5 53 1931.5 54 1658.0 55 1628.0 56 1375.5 57 991.5 58 830.0 59 683.0 60 468.5 61 324.0 62 247.0 63 203.5 64 132.0 65 91.0 66 66.5 67 55.0 68 44.5 69 32.0 70 25.5 71 21.0 72 21.5 73 18.0 74 12.0 75 10.0 76 10.0 77 10.0 78 7.0 79 4.0 80 4.5 81 3.5 82 1.0 83 0.0 84 1.5 85 2.5 86 1.0 87 0.0 88 0.5 89 1.0 90 2.5 91 3.0 92 2.0 93 1.0 94 1.0 95 1.0 96 0.0 97 0.5 98 1.0 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7144632206759444 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 48288.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.47208416169649 #Duplication Level Percentage of deduplicated Percentage of total 1 73.88701965645475 43.20328031809145 2 11.096157251638038 12.976308813783962 3 5.815477244554631 10.201292246520875 4 3.1485744643173366 7.364148442677269 5 1.9762705861519392 5.777833001988072 6 1.186470692403046 4.1625248508946315 7 0.8429254471400743 3.4501325381047057 8 0.6410483442535859 2.998674618952949 9 0.40375420577297677 2.124751491053678 >10 1.0023021073136178 7.741053677932405 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.00621272365805169 0.0 2 0.0 0.0 0.0 0.00828363154406892 0.0 3 0.0 0.0 0.0 0.00828363154406892 0.0 4 0.0 0.0 0.0 0.01035453943008615 0.0 5 0.0 0.0 0.0 0.018638170974155068 0.0 6 0.0 0.0 0.0 0.018638170974155068 0.0 7 0.0 0.0 0.0 0.018638170974155068 0.0 8 0.0 0.0 0.0 0.02277998674618953 0.0 9 0.0 0.0 0.0 0.03934724983432737 0.0 10 0.0 0.0 0.0 0.04348906560636183 0.0 11 0.0 0.0 0.0 0.05798542080848244 0.0 12 0.0 0.0 0.0 0.05798542080848244 0.0 13 0.0 0.0 0.0 0.05798542080848244 0.0 14 0.0 0.0 0.0 0.0621272365805169 0.0 15 0.0 0.0 0.0 0.07455268389662027 0.0 16 0.0 0.0 0.0 0.08076540755467197 0.0 17 0.0 0.0 0.0 0.08490722332670643 0.0 18 0.0 0.0 0.0 0.0973326706428098 0.0 19 0.0 0.0 0.0 0.10975811795891319 0.0 20 0.0 0.0 0.0 0.11389993373094764 0.0 21 0.0 0.0 0.0 0.11597084161696487 0.0 22 0.0 0.0 0.0 0.1242544731610338 0.0 23 0.0 0.0 0.0 0.13253810470510272 0.0 24 0.0 0.0 0.0 0.14082173624917163 0.0 25 0.0 0.0 0.0 0.14082173624917163 0.0 26 0.0 0.0 0.0 0.14910536779324055 0.0 27 0.0 0.0 0.0 0.1656726308813784 0.0 28 0.0 0.0 0.0 0.17809807819748177 0.0 29 0.0 0.0 0.0 0.20501988071570576 0.0 30 0.0 0.0 0.0 0.2547216699801193 0.0 31 0.0 0.0 0.0 0.2982107355864811 0.0 32 0.0 0.0 0.0 0.33548707753479123 0.0 33 0.0 0.0 0.0 0.37069251159708416 0.0 34 0.0 0.0 0.0 0.44110337972166996 0.0 35 0.0 0.0 0.0 0.540506958250497 0.0 36 0.0 0.0 0.0 0.7144632206759444 0.0 37 0.0 0.0 0.0 1.0126739562624254 0.0 38 0.0 0.0 0.0 1.4351391650099403 0.0 39 0.0 0.0 0.0 1.9445825049701788 0.0 40 0.0 0.0 0.0 2.5472166998011927 0.0 41 0.0 0.0 0.0 3.2368290258449304 0.0 42 0.0 0.0 0.0 4.206013916500994 0.0 43 0.0 0.0 0.0 5.13585155732273 0.0 44 0.0 0.0 0.0 5.926938369781312 0.0 45 0.0 0.0 0.0 6.7159542743538765 0.0 46 0.0 0.0 0.0 7.61058648111332 0.0 47 0.0 0.0 0.0 8.420311464546057 0.0 48 0.0 0.0 0.0 9.155483764082174 0.0 49 0.0 0.0 0.0 9.805748840291583 0.0 50 0.0 0.0 0.0 10.507786613651424 0.0 51 0.0 0.0 0.0 11.158051689860836 0.0 52 0.0 0.0 0.0 11.96984758117959 0.0 53 0.0 0.0 0.0 12.868621603711066 0.0 54 0.0 0.0 0.0 13.738402915838304 0.0 55 0.0 0.0 0.0 14.49635520212061 0.0 56 0.0 0.0 0.0 15.188038436050364 0.0 57 0.0 0.0 0.0 15.807239893969516 0.0 58 0.0 0.0 0.0 16.36431411530815 0.0 59 0.0 0.0 0.0 17.016650099403577 0.0 60 0.0 0.0 0.0 17.5840788601723 0.0 61 0.0 0.0 0.0 18.147365805168985 0.0 62 0.0 0.0 0.0 18.756212723658052 0.0 63 0.0 0.0 0.0 19.39198144466534 0.0 64 0.0 0.0 0.0 20.052601060304838 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTACT 15 0.0021410317 70.576805 1 TCATTGA 15 0.002213013 69.991714 25 TACAGGA 15 0.002213013 69.991714 2 AATGTAT 15 0.002213013 69.991714 46 AAGTACC 20 0.0069151805 52.493782 54 ATCTCTG 20 0.0069151805 52.493782 58 TCGTTAC 20 0.0069151805 52.493782 3 ACGTTAG 20 0.0069151805 52.493782 70 CCCACGT 20 0.0069151805 52.493782 59 AGACGTT 20 0.0069151805 52.493782 68 ACTAGGT 20 0.0069151805 52.493782 32 TCTACTG 20 0.0069151805 52.493782 2 TTGAACT 20 0.0069151805 52.493782 28 ACATGAG 20 0.0069151805 52.493782 9 CACGTAA 20 0.0069151805 52.493782 61 CCACGTA 20 0.0069151805 52.493782 60 AGTACCC 20 0.0069151805 52.493782 55 TGACTTG 20 0.0069151805 52.493782 28 TGTCCCA 20 0.0069151805 52.493782 40 ATACTGG 30 5.8266753E-4 46.66114 6 >>END_MODULE