FastQCFastQC Report
Wed 25 May 2016
SRR1780634_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780634_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48288
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT8381.7354208084824387No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT3810.7890159045725647RNA PCR Primer, Index 42 (95% over 22bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1150.23815440689198145No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC1130.234012591119947No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG730.1511762756792578No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT490.10147448641484426No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGATTC353.614332E-970.040
TCACCCG150.002211973570.030
GGTCGTT150.002211973570.011
CGTATAA150.002211973570.03
CCGATCT207.8520105E-570.034
TTCGTAT150.002211973570.01
CGTTACC150.002211973570.014
GTTACCT150.002211973570.015
AGCGGAT353.614332E-970.035
CGGATGA353.614332E-970.037
GTCGTTA150.002211973570.012
TACCTAG150.002211973570.017
CAGTGGC150.002211973570.06
GAGCCTG150.002211973570.010
GATGATT353.614332E-970.039
CAGCTCG150.002211973570.042
CGGCTCG150.002211973570.047
GCGGATG353.614332E-970.036
AGCTCGG150.002211973570.043
ACCCGAT207.8520105E-570.032
Produced by FastQC (version 0.11.5)