FastQCFastQC Report
Wed 25 May 2016
SRR1780633_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780633_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160551
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTCG207.8553805E-443.97818426
TTAGCAA207.879534E-443.9507944
GCCCAGT357.198327E-637.75441719
TTTAACG357.21377E-637.74263434
GAGGCAC357.307026E-637.672117
TTAACGG505.8524165E-835.2264635
GCGTGTA250.002338093235.2264634
GTAACTG250.002338093235.2264639
GTAACGT250.002338093235.2264624
GAGTAAC250.00234526435.20448722
AGTAACG250.00234526435.20448723
GTCATAA250.002348855835.1935161
ACGTCGG250.00235245235.1825527
TCTAATA250.002359657535.1606332
CCCAGTG401.7971603E-533.0351120
CAGTGAC401.8087152E-533.0042122
ACATGTT401.8164536E-532.9836429
GTTTAAC401.8203333E-532.97336633
CGCGGTA401.8242199E-532.96309743
GTTTGCA353.197271E-431.44238511
Produced by FastQC (version 0.11.5)