FastQCFastQC Report
Wed 25 May 2016
SRR1780633_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780633_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160551
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT20631.2849499535972995No Hit
ATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGTCTT7270.45281561622163674TruSeq Adapter, Index 3 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC3570.22235925033167028No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC3440.2142621347733742No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGG2540.1582051809082472No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCCT1990.1239481535462252No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCGT1630.1015253720001744No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATGT254.421622E-544.00685538
ATAGATT254.421622E-544.0068554
AGTTATA254.421622E-544.00685535
CCAAGCT207.8302214E-444.0068537
CATCCGG207.842249E-443.993158
TCAGTTA254.4297652E-543.9931533
CCGTCTT1200.036.68380744
ATGCCGG601.9463187E-1036.68380744
GATAGAT301.2949252E-436.6723753
CAGTTAT301.2973022E-436.66095734
CAGTCTC301.2973022E-436.66095720
GCCGTCT1100.036.01682743
GGCAGGT250.00234853435.194528
ATCCGGC250.00234853435.194529
GACCACC250.00234853435.1945217
GCTTCTT250.00234853435.1945218
CGCCATT756.002665E-1132.26164217
TGGGGTC551.3630233E-731.9950189
TTTGGGA353.1967615E-431.44326244
GCCCCAC353.208472E-431.42367711
Produced by FastQC (version 0.11.5)