FastQCFastQC Report
Wed 25 May 2016
SRR1780632_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780632_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44688
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGTTA207.848694E-569.9944161
CTTGCCA150.002211588769.99444
ACGTGTG150.002211588769.994443
TACTCGC150.002211588769.99445
AGCTCAT150.002211588769.994410
GCATCAG150.002211588769.994410
TAGTAAA150.002211588769.994421
ATTACTC352.9128205E-759.99523
TCGCATC308.253392E-658.3286678
GTATTAC252.3021738E-456.310811
CACGGGC252.3670832E-455.99551842
TTACTCG252.3670832E-455.9955184
TAAAAGT252.3670832E-455.99551866
CACTGGT252.3670832E-455.99551859
ACGGGCA252.3670832E-455.99551843
TTAAAAG252.3670832E-455.99551865
AGCTTAC200.00675874852.7913861
TAATACG200.00691075952.4958044
GGGGACA200.00691075952.4958044
TCACCTG200.00691075952.49580426
Produced by FastQC (version 0.11.5)