##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780632_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 44688 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.85105621195847 34.0 31.0 34.0 12.0 34.0 2 31.217150017901897 34.0 32.0 34.0 23.0 34.0 3 31.35374149659864 34.0 33.0 34.0 23.0 34.0 4 31.592888471177943 34.0 34.0 34.0 23.0 34.0 5 31.635427855352667 34.0 34.0 34.0 23.0 34.0 6 34.294150554958826 38.0 35.0 38.0 23.0 38.0 7 33.81373075546008 38.0 34.0 38.0 21.0 38.0 8 32.785915682062296 37.0 32.0 38.0 11.0 38.0 9 33.46596401718582 37.0 34.0 38.0 21.0 38.0 10-11 33.78720238095238 37.0 34.0 38.0 21.5 38.0 12-13 34.1421858216971 38.0 34.5 38.0 22.5 38.0 14-15 33.77916890440386 37.5 34.0 38.0 21.0 38.0 16-17 33.59462271750806 37.0 34.0 38.0 21.0 38.0 18-19 33.63144468313641 37.0 34.0 38.0 21.5 38.0 20-21 33.703242481203006 37.0 34.0 38.0 21.0 38.0 22-23 33.64728786251342 37.0 34.0 38.0 21.0 38.0 24-25 34.06319369853205 38.0 34.5 38.0 22.5 38.0 26-27 34.105375044754744 38.0 34.5 38.0 22.5 38.0 28-29 34.229849176512715 38.0 35.0 38.0 23.0 38.0 30-31 34.040402345148586 38.0 34.0 38.0 22.5 38.0 32-33 33.827571160042964 38.0 34.0 38.0 21.5 38.0 34-35 33.68211824203365 37.0 34.0 38.0 21.0 38.0 36-37 33.65404582885786 37.0 34.0 38.0 21.0 38.0 38-39 34.14945846759757 38.0 34.5 38.0 23.0 38.0 40-41 33.71237244897959 37.5 34.0 38.0 21.0 38.0 42-43 34.01916621911923 38.0 34.0 38.0 22.5 38.0 44-45 33.15139411027569 37.0 34.0 38.0 16.0 38.0 46-47 33.83769692087361 37.0 34.0 38.0 22.0 38.0 48-49 33.59936672037236 37.0 34.0 38.0 21.0 38.0 50-51 33.545616272824915 37.0 34.0 38.0 21.5 38.0 52-53 33.595976548514145 37.0 34.0 38.0 21.0 38.0 54-55 33.28288354815611 37.0 34.0 38.0 21.0 38.0 56-57 33.37108395989975 37.0 33.5 38.0 21.0 38.0 58-59 33.24552452559971 37.0 33.0 38.0 21.0 38.0 60-61 33.270922842821335 37.0 33.5 38.0 21.0 38.0 62-63 33.3562365735768 37.0 33.5 38.0 21.0 38.0 64-65 33.334687164339414 37.0 33.5 38.0 21.0 38.0 66-67 33.30429869316147 37.0 33.5 38.0 20.5 38.0 68-69 32.87840136054422 37.0 31.5 38.0 15.5 38.0 70-71 32.73028553526674 37.0 31.5 38.0 19.0 38.0 72-73 32.79039115646259 37.0 32.0 38.0 19.0 38.0 74-75 32.41494360902256 37.0 31.0 38.0 15.0 38.0 76 31.700232724668815 36.0 29.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.146739480351343 1101 2 -1.0754202041828442 1101 3 -1.313542821746946 1101 4 -3.576884924927583 1101 5 -2.21411336874478 1101 6 -3.605835611054623 1101 7 -2.8946689251340665 1101 8 -1.3654018050891317 1101 9 -1.5998351663935857 1101 10-11 -1.8874086528707608 1101 12-13 -2.679296869396161 1101 14-15 -2.239442771181764 1101 16-17 -2.2923986440451003 1101 18-19 -1.437469281229646 1101 20-21 -2.297761794584545 1101 22-23 -1.3653435701275995 1101 24-25 -2.2095198082039715 1101 26-27 -2.557699023317184 1101 28-29 -1.5745686493979605 1101 30-31 -3.4058918990950673 1101 32-33 -1.4973521197776947 1101 34-35 -2.074145103094864 1101 36-37 -2.3932645047416052 1101 38-39 -1.7705723097931028 1101 40-41 -1.5466769077458693 1101 42-43 -2.3224283371964276 1101 44-45 -2.5181669488648453 1101 46-47 -1.7295743293671464 1101 48-49 -2.135557241047181 1101 50-51 -2.427822871353584 1101 52-53 -1.9087297471462321 1101 54-55 -2.3504335211310163 1101 56-57 -3.1875199622655543 1101 58-59 -2.4361439482084464 1101 60-61 -1.793882896495866 1101 62-63 -2.5990354588417084 1101 64-65 -2.6920233460970024 1101 66-67 -2.667691993454625 1101 68-69 -1.5825465084654304 1101 70-71 -1.8334451017256583 1101 72-73 -1.856624242915892 1101 74-75 -4.000182790246079 1101 76 -2.2838344470895215 1102 1 -0.138970056792445 1102 2 -1.026798650298133 1102 3 -0.7085302904186257 1102 4 -0.153326027684475 1102 5 -1.023887804835006 1102 6 0.1202295518526455 1102 7 1.6226243079486409 1102 8 1.1263275182191421 1102 9 -0.0033439383234110664 1102 10-11 -1.2931730638983296 1102 12-13 -5.245086343080374 1102 14-15 -0.8310467812068225 1102 16-17 -2.30781217787969 1102 18-19 -0.6923564992747657 1102 20-21 -1.0393658046096022 1102 22-23 -1.2951681315311063 1102 24-25 -1.3026275776275327 1102 26-27 -6.18777421128711 1102 28-29 -1.0107841882450828 1102 30-31 -0.05551595924544728 1102 32-33 -8.021913523286472 1102 34-35 -1.4805360805384709 1102 36-37 0.3469610591681729 1102 38-39 -0.4636800792166653 1102 40-41 -1.1884062310541381 1102 42-43 -0.6322027732866502 1102 44-45 -6.455259680694418 1102 46-47 -0.9664164346303039 1102 48-49 -1.0447051107213667 1102 50-51 -7.426068485388669 1102 52-53 -2.674895160680066 1102 54-55 -0.1981778820332778 1102 56-57 -1.761580112641493 1102 58-59 -0.1249910660029343 1102 60-61 -0.580975628325433 1102 62-63 -4.988759769618653 1102 64-65 -0.7648303636408613 1102 66-67 -0.7074163042315647 1102 68-69 -7.528160543553149 1102 70-71 -0.993595477665508 1102 72-73 -1.1945941677279208 1102 74-75 -6.122864494506732 1102 76 -3.463784321776238 1103 1 -0.5225815244860712 1103 2 -1.383666429502238 1103 3 -1.1107553653009532 1103 4 -0.5153285950785715 1103 5 -0.4875163884892615 1103 6 -2.0648367724948073 1103 7 -0.36667821665555067 1103 8 1.4891858836449003 1103 9 -0.1608107761496811 1103 10-11 0.04336951205374362 1103 12-13 -1.5492155685831577 1103 14-15 -0.7913591474883788 1103 16-17 -0.8453603165189705 1103 18-19 -1.8123821732157097 1103 20-21 -0.940735081460268 1103 22-23 -0.9588395050869494 1103 24-25 -0.09755697428991539 1103 26-27 -1.8150741684972118 1103 28-29 -0.7530820059600956 1103 30-31 -0.5978779618128343 1103 32-33 -5.355888705143549 1103 34-35 -1.819325126323669 1103 36-37 -0.8422772805836516 1103 38-39 -1.0431623214075003 1103 40-41 -0.21996378347176915 1103 42-43 -0.2584329829571672 1103 44-45 -4.738956728191212 1103 46-47 -1.1360783944077966 1103 48-49 -0.654140284020464 1103 50-51 -1.4774163672885408 1103 52-53 -1.2395079120707422 1103 54-55 -0.4869027429660946 1103 56-57 0.05324658825709605 1103 58-59 0.6243670854733168 1103 60-61 -0.8057295863913367 1103 62-63 -0.5003130430461269 1103 64-65 -1.1237306631701713 1103 66-67 -1.7210020979842433 1103 68-69 -3.871977402774373 1103 70-71 -0.24413035143530237 1103 72-73 0.3461632135301045 1103 74-75 -2.329218794891844 1103 76 -0.9751236456957919 1104 1 -1.3030693293641207 1104 2 -0.20480464088435468 1104 3 0.604691789170591 1104 4 0.43589091711655215 1104 5 -0.37369525027787986 1104 6 0.07337460961900888 1104 7 1.3650291004176154 1104 8 -0.06365964481038588 1104 9 -0.04698963793830302 1104 10-11 -0.5176060977023553 1104 12-13 -0.42319930842054987 1104 14-15 0.5460392264953526 1104 16-17 -0.42666112952709767 1104 18-19 0.6794877454834705 1104 20-21 0.5470697965885094 1104 22-23 -0.2677825945178398 1104 24-25 0.4878088793686146 1104 26-27 0.8434624168686469 1104 28-29 0.22252775013745918 1104 30-31 -0.5734877179104032 1104 32-33 -0.1892220384768848 1104 34-35 0.6603496704243028 1104 36-37 0.10081215031066648 1104 38-39 -0.2464143539278254 1104 40-41 1.3775971921379906 1104 42-43 0.5342499438721049 1104 44-45 -2.7226965655895867 1104 46-47 0.5753037194133412 1104 48-49 0.6222824802071756 1104 50-51 0.3681120879960105 1104 52-53 -0.060646123452848144 1104 54-55 -0.9665775397140592 1104 56-57 0.8377994337855483 1104 58-59 0.37639960579851817 1104 60-61 -0.037436903464509896 1104 62-63 0.38993085939290495 1104 64-65 -0.33511277699130915 1104 66-67 0.6692418044547779 1104 68-69 -2.9329530125304686 1104 70-71 -0.2603905140369278 1104 72-73 -0.109121339315422 1104 74-75 -1.1097065997698934 1104 76 -0.17024559691530428 1105 1 0.3400952535422128 1105 2 0.5778010843325632 1105 3 0.6365972342754631 1105 4 0.6247957473413273 1105 5 0.7136177236921952 1105 6 1.3678338567324886 1105 7 1.5764795342496853 1105 8 1.328155617880057 1105 9 1.691481360217054 1105 10-11 1.42155493256341 1105 12-13 -0.29274224754742306 1105 14-15 0.9191006461851856 1105 16-17 0.9157246052546171 1105 18-19 1.1939032677899633 1105 20-21 0.5994370849672777 1105 22-23 0.9427796791721192 1105 24-25 0.991600639975303 1105 26-27 1.469751957074969 1105 28-29 -0.03787397152681393 1105 30-31 1.0153966163110937 1105 32-33 1.0799588045920459 1105 34-35 1.2390201630115811 1105 36-37 1.3442705092639997 1105 38-39 0.9509963176578822 1105 40-41 1.0208300950552527 1105 42-43 1.0408769849324315 1105 44-45 1.7361747878298317 1105 46-47 0.8922744101286497 1105 48-49 0.8294287533505766 1105 50-51 0.8287597616996436 1105 52-53 -0.5308585986868479 1105 54-55 0.5263171791490961 1105 56-57 0.06346042989131462 1105 58-59 1.071419077001643 1105 60-61 0.8534560390783668 1105 62-63 1.050824485135884 1105 64-65 0.6359878583847944 1105 66-67 1.0275461017834644 1105 68-69 1.885520718425326 1105 70-71 2.1477873912715353 1105 72-73 0.7489779979804752 1105 74-75 -0.32259175383151373 1105 76 -1.1953190409270569 1106 1 -0.7968647936345512 1106 2 -0.0443425099472563 1106 3 -1.6646706412958174 1106 4 -0.591922518912547 1106 5 0.42348061621762767 1106 6 -0.2131037814806902 1106 7 0.5261330798784627 1106 8 0.2052748866401899 1106 9 0.444024482729219 1106 10-11 -0.2010678007404323 1106 12-13 0.15842242884945534 1106 14-15 -0.5371871320840143 1106 16-17 -0.7157069147217925 1106 18-19 -0.2537600080466902 1106 20-21 -0.6928745765394311 1106 22-23 -0.7469225174960172 1106 24-25 -0.48245213903103945 1106 26-27 -0.12198473760289374 1106 28-29 -0.7169245391222745 1106 30-31 -0.9809545557366732 1106 32-33 0.9035250732047544 1106 34-35 1.1159551475317002 1106 36-37 1.11889088372957 1106 38-39 -0.2575397283394665 1106 40-41 -0.04805535386115878 1106 42-43 0.2713059986431787 1106 44-45 1.097371898252952 1106 46-47 0.8142853197556619 1106 48-49 0.6088036612084551 1106 50-51 0.7712999356639614 1106 52-53 -0.8898074413818193 1106 54-55 -0.29028314519116805 1106 56-57 1.3085953259549967 1106 58-59 0.4057106883830315 1106 60-61 1.5111296348324572 1106 62-63 1.0156261952936276 1106 64-65 1.125520513552118 1106 66-67 0.8715310641894831 1106 68-69 0.7349973511836936 1106 70-71 0.4450010135625604 1106 72-73 0.45014267437734645 1106 74-75 -0.7149697576646297 1106 76 -1.1743106375657106 1107 1 -0.9927712263830912 1107 2 -0.6976424264050642 1107 3 -0.6976698058739323 1107 4 -0.9578373058799663 1107 5 -1.2649070195384304 1107 6 -0.6391689443879542 1107 7 -0.3343514648166064 1107 8 0.5377727980854701 1107 9 -1.6315811981396173 1107 10-11 -0.6421705576326673 1107 12-13 -0.7388206789199714 1107 14-15 -1.645791977530969 1107 16-17 -0.4519224535689119 1107 18-19 -0.0549295986899665 1107 20-21 0.038746141923397204 1107 22-23 -0.9328038875879159 1107 24-25 -1.4920594907436566 1107 26-27 -0.15373076934893248 1107 28-29 -1.8221876970170072 1107 30-31 -1.3368442800474512 1107 32-33 0.3701081976826188 1107 34-35 -0.055097484047244905 1107 36-37 -0.7789787904558878 1107 38-39 -0.12930246852325666 1107 40-41 0.356497695428736 1107 42-43 0.3966192818511942 1107 44-45 0.2627854320875329 1107 46-47 -0.4200505198433362 1107 48-49 -0.5284143839043267 1107 50-51 0.7610660175353061 1107 52-53 0.11269882428234013 1107 54-55 -0.1401160608135541 1107 56-57 -0.24863107337666435 1107 58-59 0.2908401787756816 1107 60-61 -1.0692797218926913 1107 62-63 -1.400622760429009 1107 64-65 -0.8682138222874727 1107 66-67 0.8489746732120409 1107 68-69 0.5785646026456845 1107 70-71 -0.2826514509320077 1107 72-73 0.17988369359204626 1107 74-75 -0.8597065997698934 1107 76 -0.9758979391530112 1108 1 0.216010135624078 1108 2 0.6981589048801347 1108 3 -0.02997907366706798 1108 4 0.13855286309085102 1108 5 0.18196958056397605 1108 6 0.5148249112188026 1108 7 0.37276579068774396 1108 8 1.4330620966005796 1108 9 -0.32295344596347775 1108 10-11 1.3971919615685664 1108 12-13 1.5465095822167427 1108 14-15 1.266010377819768 1108 16-17 -0.1610238360573888 1108 18-19 0.6538517633172063 1108 20-21 0.21091716086860401 1108 22-23 -0.31426830131039196 1108 24-25 -0.8709637406156503 1108 26-27 -0.4741352752373089 1108 28-29 0.5541563890044969 1108 30-31 0.725751726096945 1108 32-33 0.92361562215779 1108 34-35 -0.15590393566014882 1108 36-37 1.0837324230617682 1108 38-39 1.1628762309135041 1108 40-41 0.7656118787364647 1108 42-43 1.5024180529725442 1108 44-45 0.0936226056666456 1108 46-47 1.3551030899876864 1108 48-49 0.9516163380618821 1108 50-51 1.0751341178937253 1108 52-53 0.03704598250046587 1108 54-55 0.2407723160425661 1108 56-57 1.342126735123081 1108 58-59 -0.2923651463651282 1108 60-61 1.2730141849173435 1108 62-63 0.1557648483779488 1108 64-65 0.9722239657309615 1108 66-67 0.6871083137650373 1108 68-69 1.3585825237926343 1108 70-71 2.4269619643265656 1108 72-73 0.8503266019381783 1108 74-75 1.4212073787247377 1108 76 1.9305280721117057 1109 1 0.33837488035356955 1109 2 -0.16645882435532044 1109 3 0.6456622776156316 1109 4 0.022665131315385167 1109 5 0.8358303882855935 1109 6 0.8604561024811872 1109 7 -0.5181409911258186 1109 8 0.5219622744009342 1109 9 0.9146005291444723 1109 10-11 1.003967425119484 1109 12-13 0.5844826581628908 1109 14-15 0.3972651403292957 1109 16-17 -0.30653437707847075 1109 18-19 0.284090523794724 1109 20-21 1.0255602901548642 1109 22-23 1.090037007300225 1109 24-25 0.8346496706111779 1109 26-27 0.34323835950996084 1109 28-29 0.6358175523268201 1109 30-31 0.7369277484489842 1109 32-33 0.5388683526632505 1109 34-35 -0.3858061454645636 1109 36-37 0.7545795147559602 1109 38-39 0.6749095049800573 1109 40-41 0.31425297601865765 1109 42-43 0.823315944768332 1109 44-45 0.04025074892293645 1109 46-47 -0.4933538569261984 1109 48-49 0.8415706179704401 1109 50-51 1.003410724446944 1109 52-53 1.1909702903490782 1109 54-55 0.41409591268975987 1109 56-57 1.1893454295604684 1109 58-59 0.5755545894743435 1109 60-61 1.115978370845717 1109 62-63 0.05806990298805914 1109 64-65 0.7431472575775402 1109 66-67 1.168153525855459 1109 68-69 1.4654849875975628 1109 70-71 1.1957262518551417 1109 72-73 -0.5667104821359885 1109 74-75 1.8929180721461165 1109 76 -0.532578354101144 1110 1 0.30854704103687425 1110 2 0.35589537289544637 1110 3 -0.755512151851871 1110 4 0.5235993365736284 1110 5 0.8338285198750768 1110 6 0.7030425164675691 1110 7 1.188785473574569 1110 8 2.606107476589642 1110 9 0.6402778279566945 1110 10-11 0.6885396925691083 1110 12-13 0.6046014356885863 1110 14-15 0.6529704652992692 1110 16-17 0.5420242776659521 1110 18-19 1.3057107026283035 1110 20-21 0.1692277130829325 1110 22-23 0.08663383100234512 1110 24-25 0.7245802725062802 1110 26-27 -0.21238021782888694 1110 28-29 0.28091964761576094 1110 30-31 0.10847452544084746 1110 32-33 1.243325846323998 1110 34-35 0.5635465837493641 1110 36-37 0.9066547850081932 1110 38-39 0.8615503697872029 1110 40-41 0.3600623981459776 1110 42-43 0.9642141163951763 1110 44-45 0.8610501617081994 1110 46-47 0.13906973842946257 1110 48-49 1.0486639050383744 1110 50-51 0.9583353207368148 1110 52-53 1.30317501475853 1110 54-55 0.1346350825816529 1110 56-57 0.33624920641887 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2116 54-55 -1.0784712879618894 2116 56-57 -1.129925545188378 2116 58-59 -0.7831882831838186 2116 60-61 -2.226846771701119 2116 62-63 -0.3794131271504071 2116 64-65 -0.5055290932234371 2116 66-67 -1.4946213859012616 2116 68-69 -1.213580991224049 2116 70-71 -4.916614231446516 2116 72-73 -0.2194731755924053 2116 74-75 -1.8755111974710417 2116 76 -1.6139658099795042 2117 1 0.6119646570413195 2117 2 0.5823471650599359 2117 3 0.28255397762854173 2117 4 -0.39304417551306514 2117 5 -0.8974857340843556 2117 6 0.06731036864873374 2117 7 -1.56906392903727 2117 8 -2.2570489557526763 2117 9 0.22530144562826848 2117 10-11 0.10587784205507944 2117 12-13 1.5297195240464632 2117 14-15 0.09705215465742612 2117 16-17 0.3443304738315547 2117 18-19 0.46783907161968585 2117 20-21 -0.2014308031715828 2117 22-23 0.5089733697631118 2117 24-25 1.2663974439427932 2117 26-27 1.2933158088451862 2117 28-29 0.5698888057152303 2117 30-31 1.064990224246067 2117 32-33 1.6387134539927999 2117 34-35 0.19900087660848698 2117 36-37 -0.9637953400265431 2117 38-39 0.8744706254137711 2117 40-41 -0.8651390466998166 2117 42-43 -1.0891340358693427 2117 44-45 1.0049790308385127 2117 46-47 0.31140927719022926 2117 48-49 -0.1263847195795762 2117 50-51 1.4855651067884992 2117 52-53 1.8564537032831225 2117 54-55 0.03578818066445422 2117 56-57 0.15307332422937492 2117 58-59 -0.27174113790399446 2117 60-61 1.9543996952293554 2117 62-63 -0.22042499826403628 2117 64-65 0.25824987794671017 2117 66-67 -0.8448192377949866 2117 68-69 0.1518797210427607 2117 70-71 0.8811528686100161 2117 72-73 0.8378332121973031 2117 74-75 -0.14522572545295276 2117 76 0.27650903456543574 2118 1 0.9890138373691855 2118 2 0.38562585358452495 2118 3 0.8481277481203442 2118 4 0.7872836933393934 2118 5 0.2090716429648225 2118 6 0.8378021719274216 2118 7 0.9719196775201127 2118 8 -3.298032562310052 2118 9 -0.29109199699468036 2118 10-11 0.13456636664524524 2118 12-13 1.4026703437185901 2118 14-15 0.6831177284279164 2118 16-17 0.8238386705528669 2118 18-19 0.07029808801313209 2118 20-21 -1.0784799834994487 2118 22-23 0.35733402550081905 2118 24-25 0.49180728000835927 2118 26-27 0.8752830219599446 2118 28-29 0.229724871289001 2118 30-31 0.47892465047558375 2118 32-33 1.1551068966157416 2118 34-35 1.6744107126740673 2118 36-37 0.21243416817018357 2118 38-39 0.9892247237744272 2118 40-41 -0.1356308499785044 2118 42-43 0.47234137396672793 2118 44-45 0.5787495226417931 2118 46-47 0.3073109165344974 2118 48-49 -0.16326996548121286 2118 50-51 0.4281880576081676 2118 52-53 -1.0656774442578651 2118 54-55 0.6382471970578933 2118 56-57 1.4399585701310187 2118 58-59 2.1339965670140373 2118 60-61 0.2904652689998386 2118 62-63 0.6730176246867856 2118 64-65 0.12300397630737336 2118 66-67 -1.3202290738605598 2118 68-69 1.8199125079280094 2118 70-71 0.02049713090509897 2118 72-73 -0.8261012140322066 2118 74-75 1.1867414876617985 2118 76 1.3092959198113405 2119 1 -0.15460697517535849 2119 2 0.31919677304282956 2119 3 0.13159175382241273 2119 4 0.12249395705999433 2119 5 0.21570029585149442 2119 6 0.09147287755821054 2119 7 -2.6843169582603608 2119 8 0.10374933362722771 2119 9 -0.7186757461037345 2119 10-11 -0.7130815307175524 2119 12-13 0.13809443495166107 2119 14-15 -0.4905814875378667 2119 16-17 -0.5050280436310217 2119 18-19 -1.0472001300909284 2119 20-21 -1.7575961631145631 2119 22-23 0.8419028066839971 2119 24-25 -0.09130034650624452 2119 26-27 -0.09952096948695299 2119 28-29 0.2010007087800929 2119 30-31 -0.8112749218693907 2119 32-33 -0.27561298934291756 2119 34-35 0.3709894724744913 2119 36-37 -0.8779435937685207 2119 38-39 -0.046555746646099294 2119 40-41 -0.5564077566071504 2119 42-43 -0.8520349624552281 2119 44-45 -1.6382497645998342 2119 46-47 -0.7208786771932338 2119 48-49 -0.5185800153743045 2119 50-51 0.04753944748699723 2119 52-53 -1.5337815069806027 2119 54-55 -1.3508475998059666 2119 56-57 0.7439500170305138 2119 58-59 0.6147877288101924 2119 60-61 0.1802371863198715 2119 62-63 -0.33795885428684613 2119 64-65 -0.18187841856077114 2119 66-67 0.14121069805676 2119 68-69 -0.19558998672630423 2119 70-71 -1.23737884913767 2119 72-73 -1.3810548847378357 2119 74-75 -0.07637326643655484 2119 76 -0.03952802887005902 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 11.0 13 87.0 14 170.0 15 309.0 16 373.0 17 497.0 18 541.0 19 515.0 20 509.0 21 465.0 22 408.0 23 401.0 24 434.0 25 512.0 26 573.0 27 677.0 28 828.0 29 900.0 30 1180.0 31 1428.0 32 1867.0 33 2507.0 34 3465.0 35 5156.0 36 8942.0 37 11930.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.91469653602578 23.69565707331365 12.575781478893871 28.813864911766697 2 16.841210168277836 22.231919083422845 36.960705334765485 23.966165413533837 3 16.22359470103831 27.544307196562833 29.99015395631937 26.241944146079483 4 12.206856426781238 19.833064804869316 39.075366988900825 28.884711779448622 5 12.050214822771215 37.531328320802004 36.1797350519155 14.238721804511279 6 24.95972073039742 39.21858216970999 19.66299677765843 16.158700322234157 7 24.33762978875761 32.27935911206588 23.549946294307198 19.833064804869316 8 21.553884711779446 35.488274257071254 20.61627282491944 22.341568206229862 9 22.433315431435734 16.284013605442176 23.062119584675976 38.22055137844612 10-11 24.201127819548873 26.067400644468314 27.276897601145723 22.454573934837093 12-13 22.984917651271033 23.739035087719298 26.696204797708557 26.579842463301112 14-15 20.65655209452202 25.881668456856428 24.27385427855353 29.18792517006803 16-17 21.914160401002505 28.283879341210167 25.741809881847477 24.06015037593985 18-19 21.42297708557107 27.35298066595059 26.269915861081273 24.954126387397064 20-21 21.304377013963478 27.712137486573578 25.984604368063014 24.998881131399926 22-23 21.98576799140709 27.285848549946294 26.45564804869316 24.272735409953455 24-25 21.55276584317938 28.109335839598998 26.053974221267456 24.28392409595417 26-27 20.863542785535266 28.300662370211242 26.747672753311853 24.088122090941642 28-29 21.83360186179735 27.300393841747223 27.883324382384533 22.982679914070893 30-31 21.377103472968134 27.640529896168992 27.176199427139274 23.806167203723593 32-33 20.283968850698177 28.68219656283566 26.993823845327604 24.04001074113856 34-35 22.03499820981024 27.321652345148586 27.187388113139992 23.455961331901182 36-37 22.275554958825637 27.168367346938776 26.36949516648765 24.18658252774794 38-39 21.302139276763338 27.47158073755818 27.948218761188688 23.278061224489797 40-41 20.936269244539922 26.486976369495167 28.03996598639456 24.536788399570355 42-43 20.825501253132835 26.508234872896523 28.176467955603297 24.489795918367346 44-45 20.150823487289653 26.692848191908343 28.506534192624418 24.64979412817759 46-47 20.62746151092016 26.194951664876477 28.920515574650913 24.257071249552453 48-49 19.791666666666664 27.040816326530614 28.75044754744003 24.417069459362693 50-51 20.06355173648407 26.962495524525597 27.938148943788043 25.035803795202295 52-53 19.25684747583244 26.950187969924812 28.829887218045116 24.963077336197635 54-55 18.509443250984607 27.369763694951665 28.552407805227354 25.56838524883638 56-57 19.156149301825995 26.99941818832796 28.601638023630503 25.242794486215537 58-59 19.596983530254207 26.6827783745077 28.171992481203006 25.548245614035086 60-61 19.499641962047978 26.48250089509488 28.250313283208023 25.767543859649123 62-63 19.39782491944146 26.42543859649123 27.913533834586467 26.263202649480842 64-65 20.32984246330111 27.160535266738272 26.540682062298604 25.968940207662012 66-67 20.06467060508414 26.651450053705695 26.779001074113857 26.50487826709631 68-69 19.893483709273184 26.903195488721803 26.583199069101326 26.62012173290369 70-71 20.92172395273899 26.573129251700678 25.9734156820623 26.53173111349803 72-73 21.0828410311493 25.85705334765485 26.122225205871825 26.937880415324024 74-75 21.11752595775152 25.999149659863946 26.26655925528106 26.616765127103477 76 22.220730397422127 24.371195846759758 26.664876476906553 26.743197278911563 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 3.0 16 3.0 17 1.0 18 2.0 19 4.0 20 10.0 21 15.0 22 16.0 23 25.5 24 30.0 25 26.0 26 66.0 27 115.0 28 157.0 29 190.0 30 242.0 31 353.0 32 495.5 33 579.0 34 690.5 35 949.0 36 1211.5 37 1327.0 38 1544.0 39 1983.5 40 2480.0 41 2840.5 42 2927.0 43 3050.5 44 3265.5 45 3374.5 46 3392.0 47 3455.5 48 3337.5 49 2997.5 50 2839.0 51 2638.5 52 2225.0 53 1802.5 54 1593.0 55 1505.5 56 1202.5 57 834.5 58 682.0 59 582.0 60 404.5 61 270.5 62 214.0 63 174.0 64 108.0 65 73.5 66 49.0 67 33.0 68 29.5 69 21.0 70 13.5 71 11.0 72 10.0 73 8.0 74 7.0 75 7.0 76 5.5 77 4.5 78 4.0 79 3.0 80 2.0 81 1.0 82 1.0 83 1.0 84 1.5 85 1.5 86 0.5 87 0.0 88 0.5 89 1.0 90 0.5 91 0.5 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7093626924453992 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 44688.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.073487289652704 #Duplication Level Percentage of deduplicated Percentage of total 1 73.73456185061752 42.0828857858933 2 10.805724367771024 12.334407447189403 3 5.740050970397961 9.828141783029002 4 3.2817094687316217 7.491944146079485 5 1.9407959223681632 5.538399570354457 6 1.2115271515389139 4.1487647690655205 7 0.9801999607920016 3.916040100250626 8 0.5920407763183689 2.703186537773004 9 0.3881591844736326 1.9938238453276045 >10 1.325230346990786 9.962406015037594 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.00447547440028643 0.0 2 0.0 0.0 0.0 0.00895094880057286 0.0 3 0.0 0.0 0.0 0.00895094880057286 0.0 4 0.0 0.0 0.0 0.011188686000716076 0.0 5 0.0 0.0 0.0 0.015664160401002505 0.0 6 0.0 0.0 0.0 0.015664160401002505 0.0 7 0.0 0.0 0.0 0.01790189760114572 0.0 8 0.0 0.0 0.0 0.01790189760114572 0.0 9 0.0 0.0 0.0 0.040279269602577876 0.0 10 0.0 0.0 0.0 0.049230218403150734 0.0 11 0.0 0.0 0.0 0.05594343000358038 0.0 12 0.0 0.0 0.0 0.05594343000358038 0.0 13 0.0 0.0 0.0 0.06041890440386681 0.0 14 0.0 0.0 0.0 0.06713211600429646 0.0 15 0.0 0.0 0.0 0.07832080200501253 0.0 16 0.0 0.0 0.0 0.07832080200501253 0.0 17 0.0 0.0 0.0 0.08503401360544217 0.0 18 0.0 0.0 0.0 0.10069817400644468 0.0 19 0.0 0.0 0.0 0.10964912280701754 0.0 20 0.0 0.0 0.0 0.11412459720730397 0.0 21 0.0 0.0 0.0 0.11860007160759041 0.0 22 0.0 0.0 0.0 0.13426423200859292 0.0 23 0.0 0.0 0.0 0.13426423200859292 0.0 24 0.0 0.0 0.0 0.14097744360902256 0.0 25 0.0 0.0 0.0 0.1476906552094522 0.0 26 0.0 0.0 0.0 0.15664160401002505 0.0 27 0.0 0.0 0.0 0.19244539921231651 0.0 28 0.0 0.0 0.0 0.23272466881489437 0.0 29 0.0 0.0 0.0 0.26629072681704263 0.0 30 0.0 0.0 0.0 0.30209452201933407 0.0 31 0.0 0.0 0.0 0.33342284282133905 0.0 32 0.0 0.0 0.0 0.37146437522377374 0.0 33 0.0 0.0 0.0 0.4453097028284998 0.0 34 0.0 0.0 0.0 0.5079663444325099 0.0 35 0.0 0.0 0.0 0.6220909416398138 0.0 36 0.0 0.0 0.0 0.8167740780522735 0.0 37 0.0 0.0 0.0 1.1479591836734695 0.0 38 0.0 0.0 0.0 1.5059971356963837 0.0 39 0.0 0.0 0.0 1.9423558897243107 0.0 40 0.0 0.0 0.0 2.46374865735768 0.0 41 0.0 0.0 0.0 3.0298961689939135 0.0 42 0.0 0.0 0.0 3.8242928750447547 0.0 43 0.0 0.0 0.0 4.526942355889724 0.0 44 0.0 0.0 0.0 5.301199427139276 0.0 45 0.0 0.0 0.0 5.950143215180809 0.0 46 0.0 0.0 0.0 6.690834228428213 0.0 47 0.0 0.0 0.0 7.198800572860724 0.0 48 0.0 0.0 0.0 7.852219835302542 0.0 49 0.0 0.0 0.0 8.510114572144648 0.0 50 0.0 0.0 0.0 9.29108485499463 0.0 51 0.0 0.0 0.0 10.002685284640172 0.0 52 0.0 0.0 0.0 10.709810239885428 0.0 53 0.0 0.0 0.0 11.347565341926245 0.0 54 0.0 0.0 0.0 12.034550662370211 0.0 55 0.0 0.0 0.0 12.524615109201575 0.0 56 0.0 0.0 0.0 13.00796634443251 0.0 57 0.0 0.0 0.0 13.556211958467598 0.0 58 0.0 0.0 0.0 14.005997135696385 0.0 59 0.0 0.0 0.0 14.520676691729323 0.0 60 0.0 0.0 0.0 15.004027926960259 0.0 61 0.0 0.0 0.0 15.556749015395631 0.0 62 0.0 0.0 0.0 15.995345506623702 0.0 63 0.0 0.0 0.0 16.521213748657356 0.0 64 0.0 0.0 0.0 16.986663086287148 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGGTTA 20 7.848694E-5 69.99441 61 CTTGCCA 15 0.0022115887 69.9944 4 ACGTGTG 15 0.0022115887 69.9944 43 TACTCGC 15 0.0022115887 69.9944 5 AGCTCAT 15 0.0022115887 69.9944 10 GCATCAG 15 0.0022115887 69.9944 10 TAGTAAA 15 0.0022115887 69.9944 21 ATTACTC 35 2.9128205E-7 59.9952 3 TCGCATC 30 8.253392E-6 58.328667 8 GTATTAC 25 2.3021738E-4 56.31081 1 CACGGGC 25 2.3670832E-4 55.995518 42 TTACTCG 25 2.3670832E-4 55.995518 4 TAAAAGT 25 2.3670832E-4 55.995518 66 CACTGGT 25 2.3670832E-4 55.995518 59 ACGGGCA 25 2.3670832E-4 55.995518 43 TTAAAAG 25 2.3670832E-4 55.995518 65 AGCTTAC 20 0.006758748 52.791386 1 TAATACG 20 0.006910759 52.495804 4 GGGGACA 20 0.006910759 52.495804 4 TCACCTG 20 0.006910759 52.495804 26 >>END_MODULE