FastQCFastQC Report
Wed 25 May 2016
SRR1780632_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780632_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44688
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT9972.2310239885427854No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT3760.8413891872538489RNA PCR Primer, Index 15 (95% over 21bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1200.26852846401718583No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC1070.23943788041532402No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG850.1902076620121733No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT490.10964912280701754No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGATTC150.002210887470.040
TATCGTA150.002210887470.038
TGATTCA150.002210887470.041
GCGAAAT150.002210887470.024
GGCGAAA150.002210887470.023
GATTGGC150.002210887470.010
GAACCGA150.002210887470.020
CGAATTG150.002210887470.024
GAATTGG150.002210887470.025
GACTGAA150.002210887470.016
AGACTGA150.002210887470.015
ATCGTAT150.002210887470.039
TGAACCG150.002210887470.019
GTGGAGC150.002210887470.011
ATATCGT150.002210887470.037
AATTGGT150.002210887470.026
GCTTAGT150.002210887470.015
CCGAATT150.002210887470.023
TGATGAA200.006908574652.530
GAGGCGC200.006908574652.513
Produced by FastQC (version 0.11.5)