##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780632_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 44688 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.92154493376298 34.0 34.0 34.0 31.0 34.0 2 33.3546142141067 34.0 34.0 34.0 33.0 34.0 3 33.53390171858217 34.0 34.0 34.0 34.0 34.0 4 33.49364482635159 34.0 34.0 34.0 34.0 34.0 5 33.54330021482277 34.0 34.0 34.0 34.0 34.0 6 37.27877730039384 38.0 38.0 38.0 36.0 38.0 7 36.887128535624775 38.0 37.0 38.0 35.0 38.0 8 36.95141872538489 38.0 37.0 38.0 35.0 38.0 9 36.78063462226996 38.0 37.0 38.0 34.0 38.0 10-11 36.533263963480124 38.0 37.0 38.0 34.0 38.0 12-13 36.39545291800931 38.0 37.0 38.0 34.0 38.0 14-15 36.648977354099536 38.0 37.0 38.0 34.0 38.0 16-17 36.77469343000358 38.0 37.0 38.0 34.5 38.0 18-19 36.7594320622986 38.0 37.0 38.0 34.0 38.0 20-21 36.50946562835661 38.0 37.0 38.0 34.0 38.0 22-23 36.601268796992485 38.0 37.0 38.0 34.0 38.0 24-25 35.96787728249194 38.0 36.5 38.0 32.5 38.0 26-27 36.16159819190834 38.0 37.0 38.0 34.0 38.0 28-29 36.06461466165413 38.0 37.0 38.0 33.5 38.0 30-31 35.79420649838883 38.0 36.5 38.0 32.0 38.0 32-33 36.248109112065876 38.0 37.0 38.0 34.0 38.0 34-35 36.29359112065879 38.0 37.0 38.0 34.0 38.0 36-37 36.42556167203723 38.0 37.0 38.0 34.0 38.0 38-39 36.025051467955606 38.0 37.0 38.0 32.5 38.0 40-41 35.6281440207662 38.0 36.0 38.0 31.5 38.0 42-43 36.03481919083423 38.0 36.5 38.0 32.5 38.0 44-45 36.22745479770856 38.0 37.0 38.0 34.0 38.0 46-47 36.47899883637666 38.0 37.0 38.0 34.0 38.0 48-49 36.323621553884706 38.0 37.0 38.0 34.0 38.0 50-51 36.41224713569638 38.0 37.0 38.0 34.0 38.0 52-53 36.321350250626566 38.0 37.0 38.0 34.0 38.0 54-55 36.49211197636949 38.0 37.0 38.0 34.0 38.0 56-57 36.545761725742935 38.0 37.0 38.0 34.0 38.0 58-59 36.60729949874687 38.0 37.0 38.0 34.0 38.0 60-61 36.29586242391694 38.0 37.0 38.0 34.0 38.0 62-63 36.55015887934121 38.0 37.0 38.0 34.0 38.0 64-65 36.57453902613677 38.0 37.0 38.0 34.0 38.0 66-67 35.969600340136054 38.0 37.0 38.0 33.0 38.0 68-69 35.35860857500895 38.0 36.0 38.0 29.0 38.0 70-71 35.303325277479416 38.0 36.0 38.0 29.0 38.0 72-73 34.90821920873613 38.0 36.0 38.0 26.0 38.0 74-75 35.46265216612961 38.0 36.5 38.0 31.0 38.0 76 34.95913891872539 38.0 36.0 38.0 27.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.34398848624456235 1101 2 0.43943719653433533 1101 3 0.08601896653138397 1101 4 0.42149631775490093 1101 5 0.27705182624484337 1101 6 -0.9366025642205642 1101 7 0.19458678505941407 1101 8 0.7258813893438685 1101 9 0.46837004403070637 1101 10-11 0.2764494461162883 1101 12-13 0.4902078685493194 1101 14-15 0.8133621446024506 1101 16-17 -0.1301356821267987 1101 18-19 0.13386568828227752 1101 20-21 0.43117739353959905 1101 22-23 1.0453832271024268 1101 24-25 -0.9647225622448303 1101 26-27 0.20382734912016076 1101 28-29 0.5768877576698159 1101 30-31 0.8681016645229818 1101 32-33 0.4688797230803985 1101 34-35 0.6636346048440274 1101 36-37 -0.14925388067782563 1101 38-39 -0.028068219699342478 1101 40-41 0.9915541008242883 1101 42-43 0.8589374160775023 1101 44-45 0.5406509938702015 1101 46-47 0.7414162778488063 1101 48-49 -1.550290395728922 1101 50-51 -1.4605437527774967 1101 52-53 -1.2731638698622447 1101 54-55 -0.48011716491982526 1101 56-57 -1.2218834454543241 1101 58-59 -1.3047592174785123 1101 60-61 -1.6575375780795483 1101 62-63 -0.6777006460008295 1101 64-65 -1.1187056020557336 1101 66-67 -1.5988718727183766 1101 68-69 -0.8339880889035314 1101 70-71 -1.6139075181520184 1101 72-73 -2.680900548192433 1101 74-75 -2.2490380926739704 1101 76 -2.7544307873246083 1102 1 -0.4271894586175904 1102 2 0.16424922660952745 1102 3 0.16947761314792587 1102 4 0.43778704457194095 1102 5 0.3118889189766776 1102 6 0.5145252553283086 1102 7 -0.008671360303992515 1102 8 0.053450311649633875 1102 9 0.5809013723515051 1102 10-11 0.4778278922315806 1102 12-13 0.8186539838375353 1102 14-15 -0.05129950953288187 1102 16-17 0.2696136913067875 1102 18-19 -0.309868647557316 1102 20-21 0.5019793985521375 1102 22-23 0.6497691920147091 1102 24-25 -4.205950632420269 1102 26-27 -0.9967992172958873 1102 28-29 0.5134792363665568 1102 30-31 1.3170991582573066 1102 32-33 0.5819123045340291 1102 34-35 0.8949629256460341 1102 36-37 -2.009278943334465 1102 38-39 0.10112977528812905 1102 40-41 0.7567170080924512 1102 42-43 0.8618196215912874 1102 44-45 0.7065657808376145 1102 46-47 1.04505036306184 1102 48-49 -2.7524207215434586 1102 50-51 -0.2535262089178545 1102 52-53 0.5052822454259811 1102 54-55 0.5713865944786676 1102 56-57 0.4660864793577062 1102 58-59 0.17468940407536593 1102 60-61 -5.872951111914137 1102 62-63 -2.3273247061512095 1102 64-65 0.15109389669112971 1102 66-67 0.9298248691362545 1102 68-69 -0.22621866534462498 1102 70-71 -1.3568899742923648 1102 72-73 -5.7641085682425555 1102 74-75 -2.6812436064584375 1102 76 -1.0551826670238569 1103 1 0.629935059996015 1103 2 0.4090074636655814 1103 3 -0.4096837047810382 1103 4 0.2972222178710453 1103 5 0.0408148924469387 1103 6 0.37930916632531364 1103 7 0.6226936375565089 1103 8 0.5607245949187813 1103 9 0.14037933555218984 1103 10-11 -0.049450670027724186 1103 12-13 -0.9863310396969069 1103 14-15 -0.7162545801362228 1103 16-17 -0.027348225680810856 1103 18-19 0.612146756807256 1103 20-21 -0.9741652313152187 1103 22-23 0.23887916206178517 1103 24-25 -2.9606575215944275 1103 26-27 -0.7087162048868123 1103 28-29 -0.3658532411687432 1103 30-31 0.6963246610386591 1103 32-33 0.34507019927087157 1103 34-35 -0.28967549968558615 1103 36-37 -1.1377556228380996 1103 38-39 -0.9389857574461473 1103 40-41 -0.4396886401745519 1103 42-43 -0.9405980078481733 1103 44-45 0.6652735258098019 1103 46-47 0.47091685856889853 1103 48-49 -2.4615563655314787 1103 50-51 -0.7963161105010812 1103 52-53 -0.4562068431491184 1103 54-55 -0.8887118222949724 1103 56-57 0.28508519566066326 1103 58-59 -0.4038300653298492 1103 60-61 -4.7397675432363435 1103 62-63 -2.0160281721332325 1103 64-65 -0.43972766941926267 1103 66-67 -0.5009624650528792 1103 68-69 -0.7073911086480109 1103 70-71 -0.07650914416827703 1103 72-73 -5.4367658211308765 1103 74-75 -3.4770288011524855 1103 76 -3.1737106944093973 1104 1 -0.004211281467398464 1104 2 0.051283886429807524 1104 3 0.24885288058481336 1104 4 -0.04424119676310312 1104 5 -0.30877860348802244 1104 6 0.09475632079686136 1104 7 0.5088724993451308 1104 8 0.43064329410577784 1104 9 0.7176151079099142 1104 10-11 -0.11042627978382313 1104 12-13 0.6965957895713828 1104 14-15 0.8325259076686535 1104 16-17 0.21655421334358493 1104 18-19 0.3519841551812348 1104 20-21 -0.466035150014406 1104 22-23 0.16164338970406078 1104 24-25 0.5149522345031414 1104 26-27 0.9132350146253856 1104 28-29 1.0853662710263805 1104 30-31 0.6231539293313375 1104 32-33 0.49547670333591753 1104 34-35 -0.021382816758759304 1104 36-37 0.8866346210643314 1104 38-39 1.1789004214156407 1104 40-41 1.3245390021018721 1104 42-43 0.6528979271111766 1104 44-45 0.7343792168667207 1104 46-47 0.4505916553168703 1104 48-49 0.03844363446852128 1104 50-51 0.08986275128754073 1104 52-53 0.47875250644437983 1104 54-55 -0.026923204408795698 1104 56-57 0.36232096801838054 1104 58-59 -0.7656186832160259 1104 60-61 0.19112676570674125 1104 62-63 -0.17456475749909117 1104 64-65 0.23506907854821435 1104 66-67 -0.08226327806100642 1104 68-69 1.3129340946040244 1104 70-71 1.2690193111162742 1104 72-73 1.315266699194332 1104 74-75 -0.3672727035915102 1104 76 -0.44200337733622774 1105 1 0.6420277019308358 1105 2 0.410305543873271 1105 3 0.3336380141504307 1105 4 0.1553898751778675 1105 5 -0.2686064370604768 1105 6 -0.020636177665942057 1105 7 0.9570517710538127 1105 8 -0.37327827452167384 1105 9 0.830274805935467 1105 10-11 0.5055810987773555 1105 12-13 -0.3882235039997042 1105 14-15 0.3357711081878847 1105 16-17 0.47910801535219605 1105 18-19 0.7489917387024505 1105 20-21 0.5292166092258768 1105 22-23 0.2602291654777815 1105 24-25 0.4932606310324843 1105 26-27 0.6158721670473284 1105 28-29 1.126187477557771 1105 30-31 1.092751524466955 1105 32-33 0.8240617959095218 1105 34-35 -0.13216371448370268 1105 36-37 0.7591494806667072 1105 38-39 0.19994298478245298 1105 40-41 0.6831507394797498 1105 42-43 0.5821867157973841 1105 44-45 1.0157210218814043 1105 46-47 1.1453378464762594 1105 48-49 0.09340708326267588 1105 50-51 0.6313329979227831 1105 52-53 0.6436428528268365 1105 54-55 1.0476139275171406 1105 56-57 0.5428224368986179 1105 58-59 0.44846207103689295 1105 60-61 0.8519862314442577 1105 62-63 -0.9889051277935508 1105 64-65 0.28801708701989526 1105 66-67 0.5316603401667663 1105 68-69 0.6295973452701418 1105 70-71 1.2443557871701145 1105 72-73 1.6591554742165329 1105 74-75 0.47617199135963517 1105 76 1.0943087084737044 1106 1 -0.462277177915837 1106 2 0.11161764766215754 1106 3 0.18702147279704917 1106 4 0.13954143053685897 1106 5 0.12767839266088288 1106 6 0.3478585886616443 1106 7 -0.649022237496979 1106 8 0.26397662743910644 1106 9 -0.6559407329116524 1106 10-11 0.5874770150386013 1106 12-13 0.875671527697186 1106 14-15 0.005718034326761767 1106 16-17 0.4099645684997668 1106 18-19 0.25153486121460844 1106 20-21 0.44934781960476755 1106 22-23 0.8646814727164625 1106 24-25 0.05282129740429298 1106 26-27 0.775130607265524 1106 28-29 0.8117248504016459 1106 30-31 0.7863974038713479 1106 32-33 -0.17247366037825174 1106 34-35 0.20636643441796565 1106 36-37 0.8635280742093911 1106 38-39 0.4607788980951426 1106 40-41 0.9102257800222802 1106 42-43 0.9407669900123352 1106 44-45 1.0004254299604227 1106 46-47 0.5099626437635933 1106 48-49 0.6467020854740824 1106 50-51 0.42191238757337146 1106 52-53 0.4921243506891386 1106 54-55 -0.36282393183712003 1106 56-57 -0.10408895923878703 1106 58-59 0.7580227374087016 1106 60-61 0.8902067828227018 1106 62-63 0.4972366973575646 1106 64-65 -0.7787306647123771 1106 66-67 0.14912311475028872 1106 68-69 0.7693953697430871 1106 70-71 0.3404784467602653 1106 72-73 0.25782125631884867 1106 74-75 -0.0628225538268552 1106 76 1.2869225961340334 1107 1 0.7662107084667866 1107 2 -0.054213597116458345 1107 3 0.4050665855790072 1107 4 -0.0864401901816052 1107 5 -0.3769164277234083 1107 6 0.14276251514451843 1107 7 0.7072851977578267 1107 8 0.3988972623597462 1107 9 -0.06655059088993198 1107 10-11 0.43041770008454705 1107 12-13 -0.09153816319671648 1107 14-15 0.13082246206277404 1107 16-17 0.5008166988255809 1107 18-19 0.6560879105045032 1107 20-21 0.6653043776665868 1107 22-23 0.31204989376909964 1107 24-25 0.14242029489803087 1107 26-27 -0.28585519056238695 1107 28-29 0.6110147417968008 1107 30-31 0.6387365851579005 1107 32-33 1.0434828976835675 1107 34-35 0.14141238262180877 1107 36-37 1.2269365955126545 1107 38-39 0.1917730501419257 1107 40-41 0.08044298971317687 1107 42-43 1.02639773353782 1107 44-45 0.7811271843463956 1107 46-47 0.8136385000710291 1107 48-49 0.8100270645885388 1107 50-51 0.5783451361113876 1107 52-53 0.3371535904552161 1107 54-55 0.4698828350801705 1107 56-57 0.6035133799425054 1107 58-59 0.5658757031564008 1107 60-61 0.9710338504918781 1107 62-63 0.7127755444753561 1107 64-65 0.6479610646109322 1107 66-67 -0.1520464758929876 1107 68-69 0.6699801650647288 1107 70-71 -0.22343132767583285 1107 72-73 0.5865597692678861 1107 74-75 -1.7077682514041328 1107 76 -0.46871650161032363 1108 1 -0.5524016827262486 1108 2 0.27361538393833484 1108 3 -0.007375810734359334 1108 4 0.3715731226704122 1108 5 0.4073897678731129 1108 6 -0.5207039467090411 1108 7 -0.1309124468914291 1108 8 -1.1204319747114297 1108 9 -0.28723671480186397 1108 10-11 0.4827090467307329 1108 12-13 1.2199697733112203 1108 14-15 0.6553360309311813 1108 16-17 -0.3686149447996101 1108 18-19 -0.047701131994223545 1108 20-21 0.8405475932323867 1108 22-23 0.03920043140350771 1108 24-25 0.99092259904549 1108 26-27 -0.3087686570242383 1108 28-29 -0.9798710748954704 1108 30-31 0.22117386114356208 1108 32-33 0.974102457335384 1108 34-35 0.5122521163647633 1108 36-37 1.042220773700052 1108 38-39 1.1375615806078798 1108 40-41 0.6114849764003196 1108 42-43 -0.08215321372281181 1108 44-45 0.674944388647468 1108 46-47 0.4472150489701576 1108 48-49 0.6169907102618666 1108 50-51 0.6343487203407818 1108 52-53 -0.11625142746592587 1108 54-55 -0.8532738469474808 1108 56-57 -0.15679127955570493 1108 58-59 0.22434984550151427 1108 60-61 1.3047512084027844 1108 62-63 1.0157709361804308 1108 64-65 0.5832529232898835 1108 66-67 1.125283273210954 1108 68-69 1.6623636776095267 1108 70-71 0.006791406579161219 1108 72-73 0.48780144873537523 1108 74-75 -0.28636528161406716 1108 76 1.143034650463406 1109 1 -0.4050178879586639 1109 2 0.21881477433711183 1109 3 0.30326681055088045 1109 4 0.32775803504024026 1109 5 -0.06295567281777181 1109 6 0.2265020477029438 1109 7 -0.17091752690159012 1109 8 0.4752627166779533 1109 9 0.6435481467678628 1109 10-11 0.49347231825727533 1109 12-13 0.32331623000413856 1109 14-15 0.6090126882844942 1109 16-17 0.77368883980796 1109 18-19 0.582347128102306 1109 20-21 1.0580990283352492 1109 22-23 0.3527323084672602 1109 24-25 -0.23637610555191202 1109 26-27 0.18792933637175224 1109 28-29 -0.5276866705266556 1109 30-31 0.5689640567239493 1109 32-33 0.7387709866724421 1109 34-35 0.4879315677236633 1109 36-37 1.1596325085235293 1109 38-39 1.111939279363277 1109 40-41 0.7208004450312586 1109 42-43 0.6837218180272586 1109 44-45 0.7817833523253981 1109 46-47 0.6814987675375903 1109 48-49 1.045997568275574 1109 50-51 0.22029614223562533 1109 52-53 -0.32535734567776586 1109 54-55 -0.23062335164649284 1109 56-57 0.555394394815643 1109 58-59 -0.07251324822467353 1109 60-61 1.0404001797007254 1109 62-63 0.4861163768713084 1109 64-65 0.7012044091928544 1109 66-67 0.6810681347806806 1109 68-69 0.05558821405860215 1109 70-71 0.6503969437902342 1109 72-73 0.44973312259872245 1109 74-75 0.6691846294857555 1109 76 0.30743647926706785 1110 1 -1.1916854443446212 1110 2 -0.26944093098785515 1110 3 0.044291767177071506 1110 4 -0.2968249090409003 1110 5 0.4113536825806037 1110 6 -0.5189270194263713 1110 7 0.3958781490626393 1110 8 0.4447675878910857 1110 9 0.7515823119887344 1110 10-11 -0.18452714791114744 1110 12-13 0.6542918072095247 1110 14-15 0.5601337345943875 1110 16-17 -0.06476037946336533 1110 18-19 -0.6115338274562205 1110 20-21 0.5502250125872195 1110 22-23 -1.3475546260049143 1110 24-25 -1.613027646990588 1110 26-27 0.8296949843098247 1110 28-29 0.8179692750225129 1110 30-31 -0.16305249205490924 1110 32-33 0.4594769789318889 1110 34-35 0.6747726193718719 1110 36-37 0.988639581791908 1110 38-39 0.5147485680327009 1110 40-41 -1.0729737442120353 1110 42-43 -0.1544604854559921 1110 44-45 0.6144605176797242 1110 46-47 0.7607060276470321 1110 48-49 1.2909928972492892 1110 50-51 -0.6481143742464255 1110 52-53 0.32538334563412263 1110 54-55 -0.35880965886656213 1110 56-57 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2116 42-43 -1.0279262949241428 2116 44-45 -0.441573965078895 2116 46-47 -0.6620730489163833 2116 48-49 -0.09671896715749284 2116 50-51 -1.721463293007389 2116 52-53 -0.26294219498539206 2116 54-55 -0.4038856378262352 2116 56-57 -0.5938046277203384 2116 58-59 -3.1733142256886993 2116 60-61 -2.7366837192946534 2116 62-63 -0.331559430894103 2116 64-65 -1.926341070036031 2116 66-67 -1.212870230682256 2116 68-69 -2.1250389920233985 2116 70-71 -0.11362016183017687 2116 72-73 -0.50163125426797 2116 74-75 -0.08475237838825933 2116 76 -2.190391378457619 2117 1 0.7414904378448739 2117 2 0.3744879381424582 2117 3 0.27157712422069125 2117 4 0.21345572446840322 2117 5 0.25839452725967504 2117 6 0.5973552812126925 2117 7 0.6345555594544194 2117 8 0.36780176405112996 2117 9 -0.12171582638303846 2117 10-11 0.028518142447282457 2117 12-13 -1.1919154725904235 2117 14-15 0.06999758566412595 2117 16-17 -0.7419623800190749 2117 18-19 -0.8278439135912592 2117 20-21 0.15268402898065858 2117 22-23 0.10576574076363698 2117 24-25 -0.8499684638841742 2117 26-27 -1.9963678109110674 2117 28-29 -1.0409498691842103 2117 30-31 -0.7182761652974747 2117 32-33 -2.482798653188148 2117 34-35 0.51820134957471 2117 36-37 -0.509207042270333 2117 38-39 1.0119005546956643 2117 40-41 0.0820693233067189 2117 42-43 0.3754089227129427 2117 44-45 -0.28308046592683667 2117 46-47 -0.6935173677405757 2117 48-49 -0.43483372125584907 2117 50-51 -0.08240167627477035 2117 52-53 0.5753138818942034 2117 54-55 -0.37145205953341787 2117 56-57 0.6522383344051619 2117 58-59 -1.0218162042076315 2117 60-61 -0.32599971703349695 2117 62-63 0.4147661767938615 2117 64-65 -0.6598063046318501 2117 66-67 -0.0555073137800548 2117 68-69 0.3471204200738356 2117 70-71 0.6093165255638269 2117 72-73 1.7054772816280206 2117 74-75 1.5914771298084673 2117 76 1.0453350206379213 2118 1 -0.0945751359256164 2118 2 -0.01895468480836371 2118 3 -0.7612097610252135 2118 4 0.008537691681517856 2118 5 0.34036174037443345 2118 6 0.408830691048756 2118 7 0.9296375266675341 2118 8 0.5973099607724421 2118 9 -0.679092875563363 2118 10-11 0.08589519162761405 2118 12-13 -0.3886367840658238 2118 14-15 1.0290139791067503 2118 16-17 -1.5493394291993923 2118 18-19 -0.20079473326338615 2118 20-21 -0.052234003806219675 2118 22-23 0.6098641014193689 2118 24-25 0.7524905525092649 2118 26-27 -1.5988268273045065 2118 28-29 -0.03275314787273231 2118 30-31 -0.2264728866089527 2118 32-33 0.32048003533645186 2118 34-35 0.16574233318126375 2118 36-37 -1.4559283537457404 2118 38-39 0.38894973502353025 2118 40-41 0.40583981510999934 2118 42-43 0.8098351522211331 2118 44-45 -1.360949318385849 2118 46-47 0.5646793535708952 2118 48-49 0.7741826721867682 2118 50-51 -0.44305741397968745 2118 52-53 -0.29353857712219167 2118 54-55 -0.3673536988776718 2118 56-57 0.64813997374943 2118 58-59 -0.2595211222404217 2118 60-61 -0.4161636514597262 2118 62-63 -0.40080759369794805 2118 64-65 0.5082264822533986 2118 66-67 0.7477713747445378 2118 68-69 -0.7594369569753425 2118 70-71 0.33472636162940006 2118 72-73 1.7423625275296644 2118 74-75 0.9439361462019065 2118 76 1.201072725555953 2119 1 0.7071354841741666 2119 2 -0.10134955294806502 2119 3 0.18084298309453573 2119 4 0.021082239079952103 2119 5 -0.3995527286205771 2119 6 -1.1701429368913736 2119 7 -0.0331564861621203 2119 8 -0.3635596044449514 2119 9 -0.023141343132856207 2119 10-11 -0.13990666154416687 2119 12-13 -0.057204139732256465 2119 14-15 0.6218507574388923 2119 16-17 0.14688295141357344 2119 18-19 0.2736172410773108 2119 20-21 -0.7236880308910401 2119 22-23 0.013499169131414135 2119 24-25 0.9570165681899567 2119 26-27 0.563040599637759 2119 28-29 -0.030151579804304163 2119 30-31 1.1602341696989598 2119 32-33 0.5494180253578307 2119 34-35 0.4232619340365744 2119 36-37 -0.5728563651498746 2119 38-39 -1.6471158387469629 2119 40-41 -0.5898123588030444 2119 42-43 0.27612168108785085 2119 44-45 0.6209822568101586 2119 46-47 -0.10723796645761041 2119 48-49 -0.4217189671574957 2119 50-51 0.8104707399761253 2119 52-53 0.22000383200111173 2119 54-55 0.15342320775098273 2119 56-57 -0.8641194702990447 2119 58-59 0.26004409515088156 2119 60-61 0.40664461653742734 2119 62-63 0.17488734571759323 2119 64-65 0.1794310439069946 2119 66-67 0.644813427488657 2119 68-69 0.27698499598260184 2119 70-71 0.022324365905951993 2119 72-73 1.0356625988054944 2119 74-75 -0.030404552301305898 2119 76 0.7374946785138974 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 3.0 23 5.0 24 20.0 25 24.0 26 53.0 27 104.0 28 205.0 29 354.0 30 540.0 31 944.0 32 1456.0 33 2206.0 34 3630.0 35 5817.0 36 10239.0 37 19087.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.450232724668815 23.681972789115648 11.103651987110634 29.764142499104906 2 16.149749373433583 20.4327783745077 40.66863587540279 22.748836376655927 3 16.212406015037594 26.022645900465452 32.131668456856424 25.633279627640533 4 12.144199785177229 21.036967418546364 39.395363408521305 27.4234693877551 5 11.918188327962763 38.283208020050125 37.44853204439671 12.350071607590404 6 24.843358395989977 42.1343537414966 18.447905477980665 14.574382384532761 7 24.729233798782673 30.301199427139274 24.189939133548155 20.779627640529895 8 21.858216970998924 38.08181167203723 20.945220193340493 19.114751163623346 9 22.560866451843893 14.757876834944502 23.328410311493016 39.35284640171858 10-11 23.94938238453276 26.791308628714646 28.094790547798066 21.16451843895453 12-13 23.228831006086644 22.690655209452203 27.525286430361618 26.555227354099536 14-15 20.508861439312568 24.710213032581454 24.39581095596133 30.385114572144644 16-17 22.993868600071607 28.188775510204085 25.58404940923738 23.23330648048693 18-19 22.83610812746151 26.935642678123884 26.11886860007161 24.109380594343 20-21 20.770676691729324 27.022914428929468 25.871598639455783 26.334810239885424 22-23 21.563954529180094 28.380102040816325 26.798021840315073 23.257921589688507 24-25 23.014008234872897 28.81646079484425 25.29873791621912 22.870793054063732 26-27 21.720596133190117 29.17114214106695 26.72529538131042 22.38296634443251 28-29 20.81655030433226 28.715762620837808 27.56892230576441 22.898764769065522 30-31 21.326754385964914 28.18206229860365 28.467373791621913 22.023809523809522 32-33 22.004788757608306 29.826799140708914 26.73200859291085 21.436403508771928 34-35 21.61766022198353 26.752148227712137 30.655880773361975 20.974310776942357 36-37 21.70716970998926 28.79408342284282 27.349624060150372 22.149122807017545 38-39 21.587450769781597 27.0374597207304 27.941505549588257 23.43358395989975 40-41 21.0750089509488 25.520273899033295 29.52470461868958 23.88001253132832 42-43 22.741004296455426 25.468805943430006 29.11855531686359 22.671634443250984 44-45 19.789428929466524 27.201933404940924 29.474355531686356 23.534282133906196 46-47 22.003669889008233 25.35580021482277 28.707930540637307 23.932599355531686 48-49 21.362558181167206 25.315520945220193 28.743734335839598 24.578186537773004 50-51 19.7312477622628 25.861528822055135 29.60190655209452 24.80531686358754 52-53 19.656283566058004 25.538175796634444 31.781462585034014 23.02407805227354 54-55 18.46468850698174 26.014813820264948 30.4500089509488 25.070488721804512 56-57 20.437253848907986 25.540413533834588 28.544575725026856 25.477756892230573 58-59 18.49713569638382 27.11578052273541 30.763292158968852 23.62379162191192 60-61 20.63529359112066 28.148496240601506 27.481650554958826 23.73455961331901 62-63 18.208467597565342 30.11322950232725 26.977040816326532 24.701262083780883 64-65 19.282581453634084 30.875179018976013 25.19580200501253 24.646437522377372 66-67 18.914473684210524 30.455603293949157 25.693698532044394 24.93622448979592 68-69 18.771258503401363 30.89419978517723 25.007832080200505 25.32670963122091 70-71 19.011815252416756 30.144557823129254 25.011188686000718 25.832438238453275 72-73 19.561179735051915 29.535893304690298 24.998881131399926 25.904045828857857 74-75 19.41908342284282 29.64554242749731 24.834407447189403 26.100966702470462 76 18.886501969208737 30.272108843537417 25.154403866809883 25.686985320443966 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 1.0 16 1.5 17 1.0 18 0.5 19 0.5 20 4.5 21 8.0 22 8.0 23 16.0 24 28.5 25 33.0 26 53.5 27 99.0 28 155.0 29 186.0 30 233.0 31 310.0 32 496.5 33 653.0 34 748.5 35 1024.5 36 1476.5 37 1748.0 38 2056.5 39 2578.0 40 2956.0 41 3147.0 42 3173.0 43 3201.5 44 3414.5 45 3614.5 46 3630.0 47 3519.0 48 3210.0 49 2822.0 50 2632.0 51 2398.0 52 1909.0 53 1491.5 54 1329.0 55 1235.0 56 926.0 57 604.5 58 498.0 59 409.5 60 253.0 61 142.0 62 99.0 63 74.5 64 34.5 65 15.5 66 9.5 67 7.0 68 5.0 69 2.0 70 0.5 71 0.0 72 0.5 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 44688.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.34863945578231 #Duplication Level Percentage of deduplicated Percentage of total 1 56.413953746325774 22.762262799856785 2 16.17769397149354 13.054958825635518 3 9.378292939936776 11.35204081632653 4 5.801120292829016 9.362692445399212 5 3.4107925239864683 6.881041890440387 6 2.434695801674893 5.8941997851772285 7 1.6194331983805668 4.5739348370927315 8 1.070378792080306 3.455066237021124 9 0.8208086073983694 2.9806659505907627 >10 2.845100105374078 15.912549230218403 >50 0.005546004104043037 0.1902076620121733 >100 0.01663801231212911 1.3493555316863588 >500 0.005546004104043037 2.2310239885427854 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT 997 2.2310239885427854 No Hit ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT 376 0.8413891872538489 RNA PCR Primer, Index 15 (95% over 21bp) ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 120 0.26852846401718583 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC 107 0.23943788041532402 No Hit TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG 85 0.1902076620121733 No Hit CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT 49 0.10964912280701754 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.00447547440028643 0.0 2 0.0 0.0 0.0 0.00895094880057286 0.0 3 0.0 0.0 0.0 0.00895094880057286 0.0 4 0.0 0.0 0.0 0.011188686000716076 0.0 5 0.0 0.0 0.0 0.015664160401002505 0.0 6 0.0 0.0 0.0 0.015664160401002505 0.0 7 0.0 0.0 0.0 0.01790189760114572 0.0 8 0.0 0.0 0.0 0.01790189760114572 0.0 9 0.0 0.0 0.0 0.040279269602577876 0.0 10 0.0 0.0 0.0 0.049230218403150734 0.0 11 0.0 0.0 0.0 0.05594343000358038 0.0 12 0.0 0.0 0.0 0.05594343000358038 0.0 13 0.0 0.0 0.0 0.06041890440386681 0.0 14 0.0 0.0 0.0 0.06265664160401002 0.0 15 0.0 0.0 0.0 0.07160759040458288 0.0 16 0.0 0.0 0.0 0.07160759040458288 0.0 17 0.0 0.0 0.0 0.07160759040458288 0.0 18 0.0 0.0 0.0 0.09174722520587182 0.0 19 0.0 0.0 0.0 0.09846043680630147 0.0 20 0.0 0.0 0.0 0.1029359112065879 0.0 21 0.0 0.0 0.0 0.10741138560687433 0.0 22 0.0 0.0 0.0 0.12083780880773362 0.0 23 0.0 0.0 0.0 0.12083780880773362 0.0 24 0.0 0.0 0.0 0.12755102040816327 0.0 25 0.0 0.0 0.0 0.13873970640887934 0.0 26 0.0 0.0 0.0 0.14321518080916576 0.0 27 0.0 0.0 0.0 0.16559255281059793 0.0 28 0.0 0.0 0.0 0.20363408521303258 0.0 29 0.0 0.0 0.0 0.2349624060150376 0.0 30 0.0 0.0 0.0 0.26852846401718583 0.0 31 0.0 0.0 0.0 0.2976190476190476 0.0 32 0.0 0.0 0.0 0.33118510562119585 0.0 33 0.0 0.0 0.0 0.40726817042606517 0.0 34 0.0 0.0 0.0 0.4721625492302184 0.0 35 0.0 0.0 0.0 0.5885248836376656 0.0 36 0.0 0.0 0.0 0.7966344432509846 0.0 37 0.0 0.0 0.0 1.1479591836734695 0.0 38 0.0 0.0 0.0 1.5104726100966703 0.0 39 0.0 0.0 0.0 1.9602577873254565 0.0 40 0.0 0.0 0.0 2.5040279269602577 0.0 41 0.0 0.0 0.0 3.0925528105979234 0.0 42 0.0 0.0 0.0 3.907089151450054 0.0 43 0.0 0.0 0.0 4.6544933762978875 0.0 44 0.0 0.0 0.0 5.457841031149302 0.0 45 0.0 0.0 0.0 6.149301825993556 0.0 46 0.0 0.0 0.0 6.907894736842105 0.0 47 0.0 0.0 0.0 7.436000716075904 0.0 48 0.0 0.0 0.0 8.102846401718582 0.0 49 0.0 0.0 0.0 8.794307196562835 0.0 50 0.0 0.0 0.0 9.604368063014679 0.0 51 0.0 0.0 0.0 10.358485499462944 0.0 52 0.0 0.0 0.0 11.117078410311493 0.0 53 0.0 0.0 0.0 11.795112781954888 0.0 54 0.0 0.0 0.0 12.495524525599713 0.0 55 0.0 0.0 0.0 13.016917293233083 0.0 56 0.0 0.0 0.0 13.50921947726459 0.0 57 0.0 0.0 0.0 14.097744360902256 0.0 58 0.0 0.0 0.0 14.583333333333334 0.0 59 0.0 0.0 0.0 15.11591478696742 0.0 60 0.0 0.0 0.0 15.626118868600072 0.0 61 0.0 0.0 0.0 16.205692803437163 0.0 62 0.0 0.0 0.0 16.67561761546724 0.0 63 0.0 0.0 0.0 17.241765127103474 0.0 64 0.0 0.0 0.0 17.74749373433584 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGATTC 15 0.0022108874 70.0 40 TATCGTA 15 0.0022108874 70.0 38 TGATTCA 15 0.0022108874 70.0 41 GCGAAAT 15 0.0022108874 70.0 24 GGCGAAA 15 0.0022108874 70.0 23 GATTGGC 15 0.0022108874 70.0 10 GAACCGA 15 0.0022108874 70.0 20 CGAATTG 15 0.0022108874 70.0 24 GAATTGG 15 0.0022108874 70.0 25 GACTGAA 15 0.0022108874 70.0 16 AGACTGA 15 0.0022108874 70.0 15 ATCGTAT 15 0.0022108874 70.0 39 TGAACCG 15 0.0022108874 70.0 19 GTGGAGC 15 0.0022108874 70.0 11 ATATCGT 15 0.0022108874 70.0 37 AATTGGT 15 0.0022108874 70.0 26 GCTTAGT 15 0.0022108874 70.0 15 CCGAATT 15 0.0022108874 70.0 23 TGATGAA 20 0.0069085746 52.5 30 GAGGCGC 20 0.0069085746 52.5 13 >>END_MODULE