FastQCFastQC Report
Wed 25 May 2016
SRR1780631_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780631_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCCACCGGCGCGAGGTCGACGACGGCGAGGGCCGGCGTCGGGGCTGCG11.282051282051282No Hit
AGCAATACACTAACACTAGTGATATATGGGCAAAACAAGATTTGAATCAC11.282051282051282No Hit
GCCGCACCATGGTGGACACCAACGTGGGCATGGCCCTGCTGAAGGACTGT11.282051282051282No Hit
GGGGTCCTCCTCGCGGACGACGACGGCGCCGCGGCCCGCGCCGAGGCGGC11.282051282051282No Hit
TTTGTAATTACTGTGGCCCCTCAGAATGATATTTGGCCTCACGGGAGGAC11.282051282051282No Hit
TAGTAGGGAGGATATGAGGTGTGAGCGATATACTAGTATTCCTAGAAGTG11.282051282051282No Hit
GTCCGGGTGCCACGGAGCCCAGCTCAGGCGGCGTTCCGGCTCGATCACGG11.282051282051282No Hit
AGCGGCGGGTGGAAAGGAGTCGGAGACAGGGAGGAAAGGAATGAAATAAA11.282051282051282No Hit
AGCCCGGCAGGTCCACGTAGATCCGCTCGACCTCCCCGGCCTCGGCGCTG11.282051282051282No Hit
CTCCAGGTGATCCCAGAGCACCCGTCACCTGGGAGCTGTCTCTTATACAC11.282051282051282No Hit
CGCTGCAGCAGCGGGCCGCCCGCTCACGCTCACGCTGCCCGACGCCGACC11.282051282051282No Hit
CTTATACACATCGGACGCTGCCGACGAATCTGGCGGTGTAGATCTCGGTG11.282051282051282No Hit
CCCCCGTGCCGCCGATCGCGGCGGCGGCCGTCAGGGGCTGTCTCTTATAC11.282051282051282No Hit
CACACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGAT11.282051282051282No Hit
GCATTTGGGAGTATGCACCCTGGTCAGACTGGAGCTGAGCAGCCTGGACC11.282051282051282No Hit
GTCCAGGCTGGTCTCAAACTCGTAACCTCAAGCCATCCTCTCACCTTGGC11.282051282051282No Hit
CTGCTCGCGGACCCCGAGGCGGACCTCGAGGCGATCATGGCCCACATCCC11.282051282051282No Hit
GCCTTCTAGCTAGTCTTCCCGCCCCAGTGTGGATCACCTCCTGTCTCTTA11.282051282051282No Hit
GCACAAACACTGTCTCTTATACACATCTGACGCTGCCGACGATTGGTACG11.282051282051282No Hit
GCCTCACGGGAGGACATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCA11.282051282051282No Hit
CACACATCTGACGCTGGCGACGCCTGACCATGTGTGGATCTCGGGGGGGG11.282051282051282No Hit
CCGCAGGGGCCGCCGGCGACGAGCTACATTGCCCTCGCAGAGGTCATTGG11.282051282051282No Hit
GGATCTTGGTGGGGAAGACATAGGGTCCAGGCGCTGGTTGATGAAATGTT11.282051282051282No Hit
GGAGCTCACTGTCTGATGGGGAAGGCAGATAGATACGGATCAATCACGCT11.282051282051282No Hit
CTGCAGGTTCGGGAACCGCTCGCGCAGCACGGAGACGATCTCGAGGGTCT11.282051282051282No Hit
GCGCTCACCCGGGCGTCGGCCAGCGCGTCGAGCGCGGCCTGCACCTGTCT11.282051282051282No Hit
GTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCAT11.282051282051282No Hit
GAGGGAGTGGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATA11.282051282051282No Hit
GTGAACCACTATCACGAAAAAAACTCTACCTCTCTATACTAATCTCCCTA11.282051282051282No Hit
GGTTATATGGGGGTAAGAGGGTTTTAAATGTATTTAGGGGGACTGCTGGT11.282051282051282No Hit
ACTTATACTAACATTAGTTCTTCTATAGGGTGATAGATTGGTCCAATTGG11.282051282051282No Hit
TAGCCCTCGTAGTAACAGCCATTCTCATCCAAACCCCCTGACGCTTCACC11.282051282051282No Hit
GGAGGTAGGTGGTAGTTTGTGTTTAATATTTTTAGTTGGGTGATGAGGAA11.282051282051282No Hit
ATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAAT11.282051282051282No Hit
CTTATACGCGTCTGACGCTGCCTTGTAATCAAGAGGTTTCGAACTCGCCA11.282051282051282No Hit
GGCTAGGGGCCGCGTGCCGCGCCCCGGTCTGGGCGTCCCCGGATGACGAC11.282051282051282No Hit
GGCCTCGGCCTCCCGCTCCTGGAAGAGCCGGTACCGTTCCGAGGCCAGGG11.282051282051282No Hit
AGTGTGAGGCGTATTATACCATAGCCGCCTAGTTTTAAGAGTACTGCGGC11.282051282051282No Hit
ATGAAGAATAGGGCGAAGGGGCCTGCGGCGTATTCGATGTTGAAGCCTGA11.282051282051282No Hit
GTCTGGGGCATGCGCCCATGGTAGGACCGACCCCGGACCTGTCTCTTATA11.282051282051282No Hit
AGTGACACGGGGATTGCAGGGGTTCTGTGGGCAAAACTAAAGGTGAATTA11.282051282051282No Hit
ATGTTGGCCTAGCATCGGGGCGACTAGCACCCCCGTGAAGCTCTACGTGT11.282051282051282No Hit
AACTTTAATAGTGTAGGAAGCTGAATAATTTATGAAGGAGAGGGGTCAGG11.282051282051282No Hit
TATACACATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGGTACG11.282051282051282No Hit
GTGGTCGCCGTCGTCGACCTGCCCCGGTTCGACGACCGGCGCGGCGTGGT11.282051282051282No Hit
GCCTCGGACGAGCCGGGCATCTCCCCCTCGGTGTGGGGCCTGTCTCTTAT11.282051282051282No Hit
ATACACATCTGACGCTGCCGACGAATGACCATGTGGCGCTATCCCTGGTC11.282051282051282No Hit
CTTATACACATCTGACGCTGCCTGTCTCTTATACACCTGTCTCTTATACA11.282051282051282No Hit
GTCTACGGAGGCTCCAGGGTGGGAGTAGTTCCCTGCTAAGGGAGGGTAGA11.282051282051282No Hit
GTATATATTGTAATTGAGATTGCTCGGGGGAATAGGTTATGTGATTAGGA11.282051282051282No Hit
GGTGGGGATAGCGATGATTATGGTAGCGGAGGTGAAATATGCTCGTGTGT11.282051282051282No Hit
GCGCCATGGCGGTGCCGGGGGCGTCCTCCTGCGTCTCGAGCACGAGCCTG11.282051282051282No Hit
CTAGAAAGAAGCGTTCTCCGAAACTCCTTTGTGATATGTGTGTTCAATGC11.282051282051282No Hit
TACCAACTATCTCCCTAATTGAAAACAAAATACTCAAATGGGCCTGTCCT11.282051282051282No Hit
CGTGTACGTCGTCGGCCGCAAGGCGCAGACGTACTTCTGTCTCTTATACA11.282051282051282No Hit
GGGTATGCACGTCTGATGCATGCAGCGAACCGGGCGGTGTTCATTTTGGT11.282051282051282No Hit
GGCCTGCAGTCCATGGTCATCGAGTACGCCCGCAACGTCCTCGGCCTGGA11.282051282051282No Hit
CCGCCAGGCCGTCCGGGTGGTCCACGCGCAGCCCGTCCACGAGCCCTTCG11.282051282051282No Hit
GCGTCATCCTCGGCGACGCCACCCTGCTGCACCGCACCCCCGTCCAGCCC11.282051282051282No Hit
CGTGAACAGCGCGTCCAGGCCCTGGGCGGCGCCCGGCAGGGTGAGGGCGA11.282051282051282No Hit
GGCCGGATGTCAGAGGGGTGCCTTGGGTAACCTCTGGGACTCAGAAGTGA11.282051282051282No Hit
GTCCGGTTTTACCTGTTGGTGGTTGGGGCGCGGAGGTATGGGCTGAGGGA11.282051282051282No Hit
ATTGGGGGGGACGGGTGGGCGGGAATTGGGGCGGAACTGGGGATTCTTCA11.282051282051282No Hit
GCTCGGATTCGTGCTGTGGTGGTTTGGAGGGTCAGGCAGGCCCCAATGGC11.282051282051282No Hit
TCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGC11.282051282051282No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG11.282051282051282No Hit
CATCTGGGTCTCGCGTGACTACGCCCGCGCCGGCAAGGGCCTGTCTCTTA11.282051282051282No Hit
GGGTGACAGTCGTCGTGGGAGCGAGAGCAGGCGGGCTTGGCGCTGAACTC11.282051282051282No Hit
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGGTGAGAATGGATGTT11.282051282051282No Hit
CCCCGGCGCGGGACAGGCTGACCTGGGCGTCCCTGGCCCCTGTCTCTTAT11.282051282051282No Hit
GCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCTCTGGTTGGGGTT11.282051282051282No Hit
GGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATTATTCCTTCTAGG11.282051282051282No Hit
ACCCCATCTCTACTAAAAATACAAAAATTAGCCAGGCATGGTGGCACATG11.282051282051282No Hit
CCCTTCCCCTACTCATCGCACTGATTTACACTCACAACACCCTCTGTCTC11.282051282051282No Hit
CCCATGCGCTCGAGCGAGGAGCTCATCCGCAGCGTGGCGACGACGGTGCG11.282051282051282No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT11.282051282051282No Hit
CCCGTGAGGTCCGCGTCGAGGTCCTTGAGGATGCGCACCACGCCGGAGGC11.282051282051282No Hit
GGGCGGTGACGGGGGTGCGCAGGCGCGCCGGGCCGCCGCGCACGATGCGC11.282051282051282No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCAAC50.044.024
CGGAGGC50.044.015
ACTGACT50.044.029
TGACTAG50.044.031
CCTAATA50.044.042
AACTGAC50.044.028
CAACTGA50.044.027
GGCAACT50.044.025
GTTCCCC50.044.037
CTGACTA50.044.030
CATCATA50.044.06
TCGGAGG50.044.014
GCAACTG50.044.026
CCCATCA50.044.04
TAGTTCC50.044.035
ATAATCG50.044.010
TTCCCCT50.044.038
ATCGGAG50.044.013
AGGCTTT50.044.018
GGCTTTG50.044.019