FastQCFastQC Report
Wed 25 May 2016
SRR1780631_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780631_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT22.564102564102564No Hit
CTCCGTGGAGGCAGCATGCTCACTCCCCATTACACACGCACAGCATCACC11.282051282051282No Hit
TCTCCCTACAAATCTCCTTAATTATAACATTCACAGCCACAGAACTAATC11.282051282051282No Hit
GCCTTGGCGTCCACGACGTCGTCGACGCCGCCACGGGCCCCGTCGCCGGC11.282051282051282No Hit
AGGGTGTTGTGAGTGTAAATCAGTGCGATGAGTAGGGGAAGGGCTGTCTC11.282051282051282No Hit
GTCCGCTGTCACACCGCCGCGGCCCTGGGCACCGCGCCCGCAGCCCCGAC11.282051282051282No Hit
CTTATACACATCTCCGAGCCCACGAACGGTAAACCATCTCGTATGCCGTC11.282051282051282No Hit
GGGCCAGGGACGCCCAGGTCAGCCTGTCCCGCGCCGGGGCTGTCTCTTAT11.282051282051282No Hit
GCTCGTGCTCGAGACGCAGGAGGACGACCCCGGCACCGCCATGGCGCCTG11.282051282051282No Hit
AATATACTCTCCGGACAATGAACCATAACCAATACTACCAATCAATACTC11.282051282051282No Hit
CCTCCGGCGTGGTGCGCATCCTCAAGGACCTCGACGCGGACCTCACGGGC11.282051282051282No Hit
GGCGTGCGCCGGGGCGTGGCCCTGGCCTCGATGGTCGGCATCCCGCTGCT11.282051282051282No Hit
TGTTTGTGCCTGTCTCTTATACACATCTCCGAGCCCAGAGACGATCCAAA11.282051282051282No Hit
CGCGTAGGCGTCGCGGACGCCGTCGAGCCCCACGATCCGGCCGCCCGAGG11.282051282051282No Hit
ATGTGTATACTGTCTCTTATACACATCTCCGAGCCCACGAGACGACGCTC11.282051282051282No Hit
CCTCAACCCCACGAGACGATCCAAAATCTCGTATGCCGTCTTCTGCTTGA11.282051282051282TruSeq Adapter, Index 6 (96% over 29bp)
CCCACGAGACCCAACACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA11.282051282051282Illumina PCR Primer Index 1 (96% over 31bp)
AAGTACGTCTGCGCCTTGCGGCCGACGACGTACACGCTGTCTCTTATACA11.282051282051282No Hit
GAGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA11.282051282051282No Hit
GGTCTCGATCAGGTCTACCGGCTCCCCGGTGACCAGGCGGACCCTGTCTC11.282051282051282No Hit
GAGGTGATCCACACTGGGGCGGGAAGACTAGCTAGAAGGCCTGTCTCTTA11.282051282051282No Hit
TCCTTCAGCAGGGCCATGCCCACGTTGGTGTCCACCATGGTGCGGCCTGT11.282051282051282No Hit
CTCCCCGAGCCTGCGATCCCACAGTGCATCTCTCCGAGCCCACGAGACAT11.282051282051282No Hit
TCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTTCTGCTTG11.282051282051282Illumina PCR Primer Index 3 (96% over 28bp)
CGCCGAGGCCGGGGAGGTCGAGCGGATCTACGTGGACCTGCCGGGCTCTG11.282051282051282No Hit
AGCTGCAGGAGTCCCTCGACGAGCAGGCCGTGCGCATCCTGGCGCTGCAG11.282051282051282No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC11.282051282051282No Hit
CTTATACACATCTGCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT11.282051282051282No Hit
ATGCACTCCAGCGTGATTGATCCGTATCTATCTGCCTTCCCCATCAGACA11.282051282051282No Hit
GTCTACCCTCCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGA11.282051282051282No Hit
GACACGGGTGAGCCTGGCTGTGCTGGGACCAGACCTGGCAGCAGGGTCCA11.282051282051282No Hit
CCGTCACCCTCCTCAAGTATACTTCAAAGGACATTTAACTCTGTCTCTTA11.282051282051282No Hit
GGATCAGACGAAGAGGGGCGTTTGGTATTGGGTTATGGCAGGGGGGTTTT11.282051282051282No Hit
CCTCTGTGCATTGAACACACATATCACAAAGGAGTTTCGGAGAACGCTTC11.282051282051282No Hit
CTTATACACATCTCCGAGACCACGAGACTTTAATGCATCTCGTATGCCGT11.282051282051282No Hit
GTCTAGACCCCGACCTCACTATCTCATCAGCTCATGGCCCACAAAGGTGT11.282051282051282No Hit
CCGCGGTCGGCGTCGGGCAGCGTGAGCGTGAGCGGGCGGCCCGCTGCTGC11.282051282051282No Hit
GTCCGGGGTCGGTCCTACCATGGGCGCATGCCCCAGACCTGTCTCTTATA11.282051282051282No Hit
TATGACATCTCCGAGCCCACGAGACTTCGCGGCATATCCTATTCCCTCTT11.282051282051282No Hit
CTCCTGGACCCCGGCCACATGCTGCACCGCCACTGACGTCTCGTTATCCG11.282051282051282No Hit
GAGGTCCCCCTCGCCGGCCACGATCTGCTCCTGCTCGGCCATGGTGGCGA11.282051282051282No Hit
GCCGAGAAAGTGTTGTGGGAAGAAAGTTAGATTTACGCCGATGAATATGA11.282051282051282No Hit
CTCCCAGGTGACGGGTGCTCTGGGATCACCTGGAGCTGTCTCTTATACAC11.282051282051282No Hit
CTGCATGCGCACGCCGAGGATGTCGAGCTTGAGGAAGCCCATGGGGTCCA11.282051282051282No Hit
GAGCAGAACCCAACCTCCGAGCCCACGAGACTAACAACAATCTCGTATGC11.282051282051282No Hit
CCCCTTAAACACCCCTCCCCACATCAAGCCCGAATGATATTTCCTATTCG11.282051282051282No Hit
AGCGGGGCCCGCGCGTCGTCATCCGGGGACGCCCAGACCGGGGCGCGGCA11.282051282051282No Hit
GCCCAGCGCATCGTGCGCGGCGGCCCGGCGCGCCTGCGCACCCCCGTCAC11.282051282051282No Hit
ATCTCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTCTTCTGCTTG11.282051282051282Illumina PCR Primer Index 7 (96% over 28bp)
CCCACGAACGATCCAAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA11.282051282051282RNA PCR Primer, Index 19 (96% over 28bp)
GGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATGGA11.282051282051282No Hit
CTTCCCACTCATCCTAACCCTACTCCTAATCACATAACCTATTCCCCCGA11.282051282051282No Hit
ATACACATCTCCGAGCCCACAGACCGGTTCCCATCTCGTATGCCGTCTTC11.282051282051282RNA PCR Primer, Index 14 (95% over 22bp)
CGCCCGAGCACACCACGGTGATCAACGACGTCGCCGAGGCCCGCACCGTC11.282051282051282No Hit
CCATAATTACCCCCATACTCCTTACACTATTCCTCATCACCCAACTAAAA11.282051282051282No Hit
CCTCATATCCTCCCTACTATGCCTAGAAGGAATAATACTATCGCTGTTCA11.282051282051282No Hit
CCCCTGACGGCCGCCGCCGCGATCGGCGGCACGGGGGCTGTCTCTTATAC11.282051282051282No Hit
GAATGAAACAACTCATTAAAAAATAATAGGCTGGGCATAGTGGCTCATGT11.282051282051282No Hit
GCCCTTGCCGGCGCGGGCGTAGTCACGCGAGACCCAGATGCTGTCTCTTA11.282051282051282No Hit
GCCCCACACCGAGGGGGAGATGCCCGGCTCGTCCGAGGCCTGTCTCTTAT11.282051282051282No Hit
ATTTATCTCCACACTAGCAGAGACCAACCGAACCCCCTTCGACCTTGCCG11.282051282051282No Hit
TCCGCGAAGGGCTCGTGGACGGGCTGCGCGTGGACCACCCGGACGGCCTG11.282051282051282No Hit
GGCCCGAGGCCCTGGCCTCGGAACGGTACCGGCTCTTCCAGGAGCGGGAG11.282051282051282No Hit
CTCTTATACACATCTCGGAGCCCACGAGACACCGGCTAATCTCGTATGCC11.282051282051282No Hit
GTGCTTCCACTCGCCCCGGGGGGTCGTGCCCAGCTCGGCCGCCTCGGCGC11.282051282051282No Hit
TAGTTATGTCATCCCTCTTATAAACATCTTCGAGCCCACGAGACCGGTTC11.282051282051282No Hit
AAAAAACCCTTCAAAAAAATCAATGAATCCAGGAGCTGGTTTTTTGAAAA11.282051282051282No Hit
GGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATATGT11.282051282051282No Hit
GTGCAGGCCGCGCTCGACGCGCTGGCCGACGCCCGGGTGAGCGCCTGTCT11.282051282051282No Hit
GTGTATAAGAGACAGGCAGCGTCAGATGTGTATAAGCTGTCTCTTATACA11.282051282051282No Hit
CGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGACATTTAA11.282051282051282No Hit
TGCCAACACAGCAGCCATTCAAGCAATCCTATACAACCGTATCGGCGATA11.282051282051282No Hit
GCTGGGATTACAGGCATGTGCCACCATGCCTGGCTAATTTTTGTATTTTT11.282051282051282No Hit
GCATAGTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA11.282051282051282No Hit
ACGCCGACCCGCAGCTGCGGCACGACGTCCGCCACCACGCCGCGCCGGTC11.282051282051282No Hit
TTCCTACACATCGGGCGAGGCCTATATTACGGATCATTTCTCTACTCAGA11.282051282051282No Hit
GTTATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAGGAG11.282051282051282No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGGC50.044.033
CGTTTGG50.044.019
AGGGGGG50.044.040
AGGGGCG50.044.014
GGGTTTT50.044.044
GTTTGGT50.044.020
TGGCAGG50.044.036
GGGTTAT50.044.030
TGGTATT50.044.023
GGCGTTT50.044.017
GCAGGGG50.044.038
AGAGGGG50.044.012
AAGAGGG50.044.011
ATTGGGT50.044.027
ATGGCAG50.044.035
GTATTGG50.044.025
GGATCAG50.044.01
CGAAGAG50.044.09
TATTGGG50.044.026
GAAGAGG50.044.010