FastQCFastQC Report
Wed 25 May 2016
SRR1780630_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780630_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44744
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCAGT252.3674847E-455.99373612
TACGGAT252.3674847E-455.9937367
GTATTGC200.00691168152.49412523
GCGGTCA200.00691168152.49412545
GAGTACT200.00691168152.49412529
CTGACTT200.00691168152.49412521
TATTGCA200.00691168152.49412524
CGGATCA200.00691168152.4941259
TTGACTC200.00691168152.49412520
TGCTCGG200.00691168152.49412510
ATTGACT200.00691168152.49412519
AAAGATG200.00691168152.49412518
ACGGATC305.8218476E-446.6614468
TTTTCTG305.8218476E-446.66144651
ATGCCAG350.00124355239.99552511
CAGATAA400.002396041534.99608614
GTGGGTT450.004267067631.1076317
TAATTCC450.004267067631.1076331
CCTGACT604.912187E-429.16340320
TGATTAA500.007141528727.99686844
Produced by FastQC (version 0.11.5)