FastQCFastQC Report
Wed 25 May 2016
SRR1780630_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780630_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44744
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT6831.526461648489183No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT3370.7531736098694797Illumina PCR Primer Index 9 (95% over 21bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1120.2503128911138924No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC820.18326479527981404No Hit
TTATAGTAGTGTGCATGGTTATTACTTTTATTTGGAGTTGCACCAAAATT480.1072769533345253No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCCGT450.10057214375111746No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACC207.845681E-570.0000160
AGGCGCC150.00221090470.014
GGGGTCG150.00221090470.09
GGCGCCA150.00221090470.015
CGCCATT150.00221090470.017
CTAAGAC252.3661769E-455.99999659
TACTTAT200.006908623552.50000447
TTACTTA200.006908623552.50000446
ATACTGG200.006908623552.5000046
TATGACA200.006908623552.50000453
ATTTTTG200.006908623552.50000448
CTTATAT200.006908623552.50000449
AGTTGAC200.006908623552.50000423
GCCTAAA200.006908623552.5000042
GCGTGAA200.006908623552.50000425
GATTCAG200.006908623552.50000442
AAGTGTT200.006908623552.50000465
GAGGCGC200.006908623552.50000413
TATAAAT404.5239765E-543.7500042
CGTCTTC1600.039.37500448
Produced by FastQC (version 0.11.5)