FastQCFastQC Report
Wed 25 May 2016
SRR1780629_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780629_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAGCGGGCCGGCGTGCTGGCTGCCGATCTCGGGCCTGTCTCTTATAC12.0408163265306123No Hit
CGCCGCGTCCCTCCGTGCACCCCTTCTAACCGGAAGGGGGTTGGTAATCC12.0408163265306123No Hit
CTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGTCAGGGAG12.0408163265306123No Hit
CCGCCCGCGAGCCGCTCGCGGGCGGCCACCCGGTTGCGCCGCTGGGCCCG12.0408163265306123No Hit
GGATATCACCTATCCACTTCCGCCTCCTGAGGGACCCCTGCTGGATATCT12.0408163265306123No Hit
TCATCCGCCTGTCCACGTGGGGGATCCTGGCCTGGCTGGTGGGTCAGGCG12.0408163265306123No Hit
CTTATACACATCTGACGCTGCCGACGATTGGTACGGTGTAGATCTCGGTG12.0408163265306123No Hit
CCAGCACGCCGGCCCGCTGCGCGACGGCCTGCGCTCGCTGCAGCTGGCGT12.0408163265306123No Hit
TCCGCGACGTCGGGGCCCGCCACGACCGTGAGCGTCACCTCTGTCTCTTA12.0408163265306123No Hit
CCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGC12.0408163265306123No Hit
CCGATGTTCGTGGCGGCGGGCTGCTGCAGGGTGGGGACTGTCTCTTATAC12.0408163265306123No Hit
AGCTCGTAGTTACCATCGCATCGTCTGTTCGTTTCGTTTTACCATTGGAG12.0408163265306123No Hit
GGGTTGCGGTGGGTCATCGGGGGTCCTTCTGGGACTGTCTCTTATACACA12.0408163265306123No Hit
ATCCCCACCGGCGTCAAAGTCTTTAGCTGACTCGCCACACTCCACGGAAG12.0408163265306123No Hit
CAGTGAGAGTGATTGTAAACATTCAAAGGACACGAGAAAAGCAAAAACAA12.0408163265306123No Hit
CCTGTAATCCCAGCACTTTGGGAGGCTGAGGCAGGGGGATTACATGAGGT12.0408163265306123No Hit
TAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATTTCGGT12.0408163265306123No Hit
CGTGAAGACCGTGACCCGGCACCGCAGCTCCGTCCCCGCGATGCACACGG12.0408163265306123No Hit
CTCTACCTCGACGCCGGCCCCCGCGAGAGCCGGGATCTGTCTCTTATACA12.0408163265306123No Hit
GTCTGGCCCAGGCGCAGCCAGGCCCAGAACATCACCTGTCTCTTATACAC12.0408163265306123No Hit
GCCCACGATAGCGCCTACGGTTCCGAGGCCGCGAGCTGTCTCTTATCCAC12.0408163265306123No Hit
TTGCCCTCCGGTCCCTGCGCTTAGGTTGCATACGGGACGAGGTCACCGTC12.0408163265306123No Hit
GGTGTTGGAGGGCCGGGTGATGTTGACGCACGGCAGGCCTTGGCTCTTAC12.0408163265306123No Hit
GCACCCGTGAGCGCACATTACGTGCCGGTGGACTGGGAGCCTCACTCCTG12.0408163265306123No Hit
ACCAGGCATCGTACAGACGGGATTGCCGAAAAGGGCGGGAGATATGCCGG12.0408163265306123No Hit
ATCCCCCCCGGTGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAG12.0408163265306123No Hit
GACGCCTCGTCCGCAAACCCAGAGGGCGGGTCGCCAACAGGGACAGGGGA12.0408163265306123No Hit
AATAATGATGTCTGCTTTGTTTCTGTTGCGAGTGGGTTTAGTAATGGGGT12.0408163265306123No Hit
CCTATACACCCTCGACACTCTCGCCGGAGTCCCCAGCCGCCCCCCCCGCC12.0408163265306123No Hit
GGCTAGGCCGGAGGTCATTAGGAGGGCTGAGAGGGCCCCTGTTAGGGGTC12.0408163265306123No Hit
AGCTATCACCAGGCTCGGTAGGTTTTTCGCCTCTACCTATAAATCTTCCC12.0408163265306123No Hit
ATTTTATCTTTTGGCGGTATGCACTTTTAACAGTCACCCCCCAACTAACA12.0408163265306123No Hit
CTTGACCACCATCCTCCGTGAAATCAATATCCCGCACCAGAGGGCTACTA12.0408163265306123No Hit
CTGTCGGCGCAGGCCGCGCAGCTCTCCCGCGCGGAGCTCTCCCGCCTCGC12.0408163265306123No Hit
CACCAGTCTCACCCCGTGAAGGCCGCGGGCGACCAGATCAGCGAAGAACC12.0408163265306123No Hit
ATCTAACACATGTCTGTGTTGCCTCCAGAAACTAAGTGTGATGGAGAGCT12.0408163265306123No Hit
ACTTTTACGCGTCATTGGCGGGCGAGGAGCGGATCGGTCAGCCATAATTT12.0408163265306123No Hit
CGCTGTGCCTGGACATCTCCCACTCCAAGCTCTCGGCCACGTTCCTGGGG12.0408163265306123No Hit
GGATCCTCCCGCATCAACCCTCACCCCTCTCCTTCATAAATTCTTCAGCT12.0408163265306123No Hit
GATTTTGCGTAGCTGGGTTTGGTTTAATCCACCTCAACTGCCTGCTATGA12.0408163265306123No Hit
CTCCCATATTGTAACTTACTACTCCGGAAAAAAAGAACCATTTGGATACA12.0408163265306123No Hit
CCTATTGCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGC12.0408163265306123No Hit
CTTATACCCATCTGACGCTGCCGACGCCAATGATCCCCCCCACCTCGCAG12.0408163265306123No Hit
GCCTCGTCCGATCCCTATGAGGCATACTTCTAACACGCCCCATCTGCCTC12.0408163265306123No Hit
CGGCGTCCACCCCTCGGACTTCACGGAGCTCGGGGACCTCGCCAAGTTCC12.0408163265306123No Hit
CCCCATTCCTGATGTTTCATTCCATGTGAAGGGTATTCCATTATGGGATT12.0408163265306123No Hit
CCTCAAGGACCTGGCCGGCCGCCGCTGGTCCGCGGAGCTGTCTCTTATAC12.0408163265306123No Hit
CCCTAGAGAAACAGTGCTAGAAGTCAGTGGCAAGAGCAGCAGCTGTCTCT12.0408163265306123No Hit
CCACAACCCTCCTCCACCCCACCCCCACCCCTCCCCCCCAGCCCGCACAC12.0408163265306123No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers