FastQCFastQC Report
Wed 25 May 2016
SRR1780629_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780629_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT24.081632653061225No Hit
AATCAATATCCCGCACAAGAGTGCTACTCTCCTCGCTCCGAGCCCACGAG24.081632653061225No Hit
ATGCACGTGCGTGCGCGCGCGCGCACACACACACGCTTTGCTCTTCCACA12.0408163265306123No Hit
CTCAAGGACAGGGTGAGCGCGGAACCACCATAGGAGATGAAGGGCAGGGG12.0408163265306123No Hit
GAGTGAGGCTCCCAGTCCACCGGCACGTAATGTGCGCTCACGGGTGCCTG12.0408163265306123No Hit
ATCTCCGAGCCAACGAGACATGATGATATCTCGTATGCCGTCTTCTGCTT12.0408163265306123RNA PCR Primer, Index 25 (96% over 28bp)
GATCTGACTGGCTCTGCGGCGTGTGATTTTGAGCTCTCCATCACACTTAG12.0408163265306123No Hit
CTTATACACATCTCCGAGCCCACGAACCGGTTCCCATCTCGTATGCCGTC12.0408163265306123No Hit
GAGGAAGAAGGGAGAGGGGACATGAGGCATTTTTTTTGGTTTGTTTTTGC12.0408163265306123No Hit
ACCGTGAGCTTCTACTGCCGGGCGCGGGCGTTCTTCGCCGCCCACGGCAT12.0408163265306123No Hit
ATCCCGGCTCTCGCGGGGGCCGGCGTCGAGGTAGAGCTGTCTCTTATACA12.0408163265306123No Hit
CGTCCAGCGCGCCCGCGTCCTCGCACGGCTGGCACCCCGGCTGGCCGGCA12.0408163265306123No Hit
CACGCGGCCTGCGGACCGTAGACGCTGTCGTGGGCCTGTCTCTTATACAC12.0408163265306123No Hit
CATCCTCGCCTTAGCATGATTTATCCTACACTCCGAGCCCACGAGACTTC12.0408163265306123No Hit
GACCATACCTATGTATCCAAATGGTTCTTTTTTTCCGGAGTAGTAAGTTA12.0408163265306123No Hit
AGGTGACGCTCACGGTCGTGGCGGGGCCCCCCCGCGCGGGCTGTCTCTTT12.0408163265306123No Hit
GCGCAGCAAGGCATACAAGGCGGCCAAGGCCGCGATCGGCGATGATCTGT12.0408163265306123No Hit
GTGATGTTCTGGGCCTGGCTGCGCCTGGGCCAGACCTGTCTCTTATACAC12.0408163265306123No Hit
GCATACATCATTATTCTCGCACGGGCTACAACCACGACCAATGATATGAA12.0408163265306123No Hit
GGCCTGATCGGCCTGCTGCACCGCCGGTGGGTGATCCCCCGCCTCGACGC12.0408163265306123No Hit
GGCGTGGAGAGGATGGCGCGCGGCTGGAAGTCGGTGATCAGCTGCACCTG12.0408163265306123No Hit
TCCTTATACACATCTCCGAGCCACGAGACATGATGATATCTCGTATGCCG12.0408163265306123No Hit
ATCTCCGCCCACGAGACATGATGATATCTCGTATGCCGTCTTCTGCTTGA12.0408163265306123TruSeq Adapter, Index 25 (96% over 29bp)
TCCGGGAAGGGGGAGGCCTCGCGGAACGCCAGCTGCAGCGAGCGCAGGCC12.0408163265306123No Hit
TCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTCTTCTGCTTG12.0408163265306123RNA PCR Primer, Index 36 (96% over 28bp)
GCCCGAGATCGGCAGCCAGCACGCCGGCCCGCTGCGCCTGTCTCTTATAC12.0408163265306123No Hit
CTTATACACATCTCCGAGCCCACGAGAATGATGATATCTCGTATGCCGTC12.0408163265306123No Hit
CGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTG12.0408163265306123No Hit
AAATTATAACAAACGGTCTCTCAGACCACAGTGCAATCAAACTAGAACTC12.0408163265306123No Hit
CTGCTGCTCTTGCCACTGACTTCTAGCACTGTTTCTCTAGGGCTGTCTCT12.0408163265306123No Hit
TTTGGTTTCCCAGTCCACATCTCCGAGCCACGAGACAAACGGTCATCTCG12.0408163265306123No Hit
CCATGTCCGTCAGCGCGAAGTTGGTGACGTCCGCGAGGCGGGAGAGCTCC12.0408163265306123No Hit
ATATCCAGCAGGGGTCCCTCAGGAGGCGGAAGTGGATAGGTGATATCCCT12.0408163265306123No Hit
GCGATGGCCTGCGGGGGCCGTGCGCGGTGCCGTCCGCGGCGGGCGGGGCG12.0408163265306123No Hit
GATTAGTAGTATGGGAGTGGGAGGGGAAAATAATGTGTTAGTTGGGGGGT12.0408163265306123No Hit
CCCCAGGAACGTGGCCGAGAGCTTGGAGTGGGAGATGTCCAGGCACAGCG12.0408163265306123No Hit
ACGCCGTGGTCCTCCGACTCCCCCGGCTCCGTGATCTCCAGCCGCCCTCC12.0408163265306123No Hit
TCCCAGAAGGACCCCCGATGACCCACCGCAACCCCTGTCTCTTATACACA12.0408163265306123No Hit
CTCCGCGGACCAGCGGCGGCCGGCCAGGTCCTTGAGGCTGTCTCTTATAC12.0408163265306123No Hit
TCTCCACCCTGCAGCAGACCGCCGCCACGAACATCGGCTGTCTCTTATAC12.0408163265306123No Hit
ATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAA12.0408163265306123No Hit
CTTATACACATCTCCGAGCCCACGGGACTTTAATGCATCTCGTATGCCGT12.0408163265306123No Hit
GTCCCTTGACCACCATCCTCCGTGAAATCAATATCCCGCACAAGAGTGCT12.0408163265306123No Hit
TCCGTGTGCATCGCGGGGACGGAGCTGCGGTGCCGGGTCACGGTCTTCAC12.0408163265306123No Hit
AAATAAACCCTCGTTCCACAGAAGATGCCATCAAGTATTTCCTCACGCAA12.0408163265306123No Hit
GGATTTTACCCTGTTGGCCTTGAACTGCTGACCTCATGTAATCCACCTGC12.0408163265306123No Hit
GTTCTTCGCTGATCTGGTCGCCCGCGGCCTTCACGGGGTGAGACTGGTGC12.0408163265306123No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers