FastQCFastQC Report
Wed 25 May 2016
SRR1780628_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780628_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90025
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCAA150.002156200870.501961
AGCGGGC207.893072E-569.9927822
GAGCGGG207.893072E-569.9927821
GACCATA150.002219137369.9927756
GGGAATA150.002219137369.99277521
GAACCTC150.002219137369.99277532
CTCGTTA252.3803872E-455.9942254
GTTAACC252.3803872E-455.9942257
GCCCCAC200.00693416852.49458711
GGGACTT200.00693416852.49458711
AGACCAT200.00693416852.4945875
GTTCCTA200.00693416852.49458725
GAATTGA200.00693416852.49458727
AAGATAC200.00693416852.49458726
TATGAGC305.853384E-446.6618518
TGAGCGG305.853384E-446.6618520
CTAGGGA305.853384E-446.661854
ACAATTC350.001250247139.99587221
TACTGAC350.001250247139.99587234
CACAATT350.001250247139.99587220
Produced by FastQC (version 0.11.5)