##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780628_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 90025 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.761832824215496 34.0 31.0 34.0 12.0 34.0 2 31.24951957789503 34.0 32.0 34.0 23.0 34.0 3 31.709680644265482 34.0 33.0 34.0 23.0 34.0 4 32.02292696473202 34.0 34.0 34.0 27.0 34.0 5 31.539505692863095 34.0 33.0 34.0 23.0 34.0 6 34.80393224104415 38.0 36.0 38.0 27.0 38.0 7 34.064670924743126 37.0 34.0 38.0 24.0 38.0 8 33.10522632602055 37.0 34.0 38.0 21.0 38.0 9 33.95039155790058 37.0 34.0 38.0 23.0 38.0 10-11 34.360755345737296 37.0 34.5 38.0 25.0 38.0 12-13 34.611130241599554 38.0 35.0 38.0 25.5 38.0 14-15 34.34059427936684 38.0 34.0 38.0 24.5 38.0 16-17 34.20350458206053 38.0 34.0 38.0 24.0 38.0 18-19 34.17229103026937 37.0 34.0 38.0 24.0 38.0 20-21 34.24493751735629 37.5 34.0 38.0 24.0 38.0 22-23 34.19023049153013 37.0 34.5 38.0 24.0 38.0 24-25 34.713474034990284 38.0 35.5 38.0 26.5 38.0 26-27 34.745337406276036 38.0 36.0 38.0 26.0 38.0 28-29 34.96454873646209 38.0 36.0 38.0 27.0 38.0 30-31 34.86646487086921 38.0 36.0 38.0 26.5 38.0 32-33 34.44959733407387 38.0 35.0 38.0 25.0 38.0 34-35 34.44141627325743 37.5 35.0 38.0 25.0 38.0 36-37 34.461427381282974 37.5 34.5 38.0 25.0 38.0 38-39 34.970841432935295 38.0 36.0 38.0 27.0 38.0 40-41 34.39582893640656 37.5 34.5 38.0 24.5 38.0 42-43 34.72314357123021 38.0 36.0 38.0 26.5 38.0 44-45 33.68931407942239 37.0 34.0 38.0 22.5 38.0 46-47 34.49926131630103 37.0 34.5 38.0 26.0 38.0 48-49 34.249358511524576 37.0 34.0 38.0 24.0 38.0 50-51 34.05321855040266 37.0 34.0 38.0 24.0 38.0 52-53 34.05175229103027 37.0 34.0 38.0 24.0 38.0 54-55 33.80750902527076 37.0 34.0 38.0 23.5 38.0 56-57 33.91858928075535 37.0 34.0 38.0 23.5 38.0 58-59 33.81330186059428 37.0 34.0 38.0 23.0 38.0 60-61 33.85443487920023 37.0 34.0 38.0 23.5 38.0 62-63 33.89416828658706 37.0 34.0 38.0 24.0 38.0 64-65 33.96691474590392 37.0 34.0 38.0 24.0 38.0 66-67 33.86012774229381 37.0 34.0 38.0 23.5 38.0 68-69 33.3023715634546 37.0 32.5 38.0 22.0 38.0 70-71 33.34891419050264 37.0 33.0 38.0 22.0 38.0 72-73 33.37710080533185 37.0 33.0 38.0 22.5 38.0 74-75 32.86680366564843 37.0 31.5 38.0 20.5 38.0 76 31.985981671757845 36.0 30.0 37.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.852112250901744 1101 2 -1.006741472564073 1101 3 -0.7880185084228799 1101 4 -3.1013361755383713 1101 5 -0.9078798810444333 1101 6 -3.4745279047469033 1101 7 -1.3423066010850846 1101 8 -0.6714140755916063 1101 9 -0.8258221608846128 1101 10-11 -1.6006174883110447 1101 12-13 -1.4939734880259223 1101 14-15 -0.7157807877458637 1101 16-17 -2.3700933462996865 1101 18-19 -0.55051792272927 1101 20-21 -1.7299209117879641 1101 22-23 -0.8002342994070375 1101 24-25 -2.4379750152427135 1101 26-27 -1.9751493949683905 1101 28-29 -0.5979277364648965 1101 30-31 -1.6916829114543788 1101 32-33 -0.6209612156392694 1101 34-35 -1.5477033520688295 1101 36-37 -0.49852813040293853 1101 38-39 -1.2743847523728675 1101 40-41 -0.23662505163565584 1101 42-43 -1.4046013954005971 1101 44-45 -0.9209800553263676 1101 46-47 -0.12132792357116529 1101 48-49 -2.2467663209404947 1101 50-51 -1.4501031372427136 1101 52-53 -0.626316763666658 1101 54-55 -2.606811928738999 1101 56-57 -1.2442991336874627 1101 58-59 -1.263128539300375 1101 60-61 -0.14094821967783133 1101 62-63 -2.5419548952623607 1101 64-65 -0.16609770395307777 1101 66-67 -1.0976935536333485 1101 68-69 0.11247520150590873 1101 70-71 -0.06337018770341274 1101 72-73 -0.9221913903476349 1101 74-75 -2.242222377170222 1101 76 -3.3501550645288134 1102 1 0.8390555771563548 1102 2 -0.4194971885688865 1102 3 0.04447526483992803 1102 4 0.30086906891353493 1102 5 0.8345011113155714 1102 6 0.6610750393104112 1102 7 1.4117022982516616 1102 8 1.732484281669187 1102 9 1.2072844912296858 1102 10-11 0.1883380989612249 1102 12-13 -4.053568260365211 1102 14-15 -1.691657428386172 1102 16-17 -2.7367502180988623 1102 18-19 -1.2542931445130492 1102 20-21 -0.5033166524198691 1102 22-23 -1.3357826720597714 1102 24-25 0.21083001092357279 1102 26-27 -6.386610789197817 1102 28-29 -0.39610449184507956 1102 30-31 0.06542536380938202 1102 32-33 -7.345697453850448 1102 34-35 -1.0674819880635695 1102 36-37 9.611372208269131E-4 1102 38-39 0.27894130477319123 1102 40-41 0.5483356289677062 1102 42-43 0.08553377377075577 1102 44-45 -6.201316159207931 1102 46-47 -0.5424372622776801 1102 48-49 0.1375423013414263 1102 50-51 -6.7944976745051875 1102 52-53 -1.5843037784708969 1102 54-55 0.9343754890540765 1102 56-57 -1.337294404165732 1102 58-59 0.7744977445269043 1102 60-61 -0.2915932816653566 1102 62-63 -5.447483398326753 1102 64-65 -1.1968815732055944 1102 66-67 0.056701543335840654 1102 68-69 -7.124301727383354 1102 70-71 -0.6260223579661925 1102 72-73 0.3280884630092089 1102 74-75 -7.366967010982105 1102 76 -2.434488873612853 1103 1 0.10446708380534986 1103 2 -0.6533659451379101 1103 3 -0.10849736231356744 1103 4 0.17461809784010285 1103 5 0.6685846464798537 1103 6 -0.9862539375209209 1103 7 0.853380770124808 1103 8 1.6357196698700704 1103 9 0.9409818818982387 1103 10-11 0.9381667330607542 1103 12-13 -0.922096341524103 1103 14-15 -0.46818486977903007 1103 16-17 -0.6299549889255331 1103 18-19 -0.4729617457103217 1103 20-21 -0.46815808183997376 1103 22-23 -0.544175031425489 1103 24-25 0.665989266967074 1103 26-27 -0.20558556413726592 1103 28-29 0.5072508524861377 1103 30-31 0.42810415556554204 1103 32-33 -5.609321042937864 1103 34-35 -1.0871376568475597 1103 36-37 0.1075335907235413 1103 38-39 0.8285860061395454 1103 40-41 0.7366724657816661 1103 42-43 0.6773459110462667 1103 44-45 -4.859001348624375 1103 46-47 -0.02148492484679565 1103 48-49 0.14177503908305056 1103 50-51 -0.07177912586011814 1103 52-53 -0.6426424431878033 1103 54-55 0.6301496059484748 1103 56-57 0.7078890715917083 1103 58-59 0.9303264700215763 1103 60-61 1.0506301337712927 1103 62-63 0.3519956493358123 1103 64-65 0.769016612600744 1103 66-67 -0.09802472819914243 1103 68-69 -3.8747929762980355 1103 70-71 0.4180472394381809 1103 72-73 0.8780244864063604 1103 74-75 -1.6647152630499207 1103 76 0.07757528578028428 1104 1 -3.201646401000783 1104 2 -0.16341865447784798 1104 3 0.33707625107141226 1104 4 0.0916308359305873 1104 5 -0.9412963581921083 1104 6 -0.6514897319544914 1104 7 1.285747100037355 1104 8 0.6108224930142683 1104 9 -0.5321044096802439 1104 10-11 -1.1735211637567247 1104 12-13 -1.0020188747669252 1104 14-15 -0.3973766599000257 1104 16-17 -1.3591807206669415 1104 18-19 0.2518565667921706 1104 20-21 0.5370379809320553 1104 22-23 -0.1981202456570088 1104 24-25 0.25460684473331696 1104 26-27 0.3349097838641697 1104 28-29 -1.1696698439160542 1104 30-31 -1.603201997850583 1104 32-33 -1.2704489030185258 1104 34-35 -0.2669034596667075 1104 36-37 -0.7084479210761572 1104 38-39 -0.4644050492451939 1104 40-41 0.5893109276272952 1104 42-43 0.5496205985837008 1104 44-45 -4.265996317278287 1104 46-47 -0.01236061865345306 1104 48-49 0.5803556803800234 1104 50-51 0.9467190572649073 1104 52-53 -0.3541736095816006 1104 54-55 -0.6524493238922062 1104 56-57 0.3584201581228754 1104 58-59 0.14695387089208367 1104 60-61 -0.19058996765426173 1104 62-63 0.259215231654224 1104 64-65 -0.5302187026570166 1104 66-67 -0.11350310539037167 1104 68-69 -3.850275146964492 1104 70-71 -1.2658314891486526 1104 72-73 -0.17060187624986867 1104 74-75 -1.4546915039156332 1104 76 -0.4561267467238075 1105 1 -1.1006808520185878 1105 2 3.5072601748709076E-4 1105 3 -0.5303549283941358 1105 4 -0.24176641987757108 1105 5 -0.41141419927444645 1105 6 -0.027094997887971317 1105 7 1.0837201183607306 1105 8 0.3937705336306081 1105 9 0.7841776034941716 1105 10-11 0.715474262590206 1105 12-13 -1.903436036033746 1105 14-15 -0.2104321861577816 1105 16-17 0.9000169244290959 1105 18-19 0.6424676336902309 1105 20-21 -0.5216140858413567 1105 22-23 -0.02565900664767895 1105 24-25 0.2851170419661173 1105 26-27 0.1299199954795256 1105 28-29 -1.187552156247662 1105 30-31 -0.7558248967987424 1105 32-33 0.11861843611256973 1105 34-35 -0.2932618921597765 1105 36-37 0.21647033805916038 1105 38-39 0.04312661635076864 1105 40-41 -0.07116005316719765 1105 42-43 0.302403623331287 1105 44-45 1.4229856853489267 1105 46-47 0.3921967341890209 1105 48-49 0.07305413569341823 1105 50-51 -0.6105906375372356 1105 52-53 -1.1691468100840368 1105 54-55 -1.044896563879547 1105 56-57 -0.07983552538416916 1105 58-59 -0.21033760600473528 1105 60-61 0.2586300183467216 1105 62-63 0.6138555239077803 1105 64-65 0.3308745634298731 1105 66-67 -0.12017181757622808 1105 68-69 0.7621005637735294 1105 70-71 1.067700003856629 1105 72-73 -0.2629762446159134 1105 74-75 -1.6221380247751327 1105 76 -1.5497380149776703 1106 1 -0.2645420824975737 1106 2 0.02199415961983675 1106 3 -0.8582136434135244 1106 4 -0.056481858510835536 1106 5 0.17423719994995324 1106 6 0.168481985656733 1106 7 0.8341970075757033 1106 8 0.919176675544449 1106 9 1.4381446928978008 1106 10-11 0.3641379246801293 1106 12-13 0.17866751705293638 1106 14-15 0.16422455841826178 1106 16-17 0.6330742559457008 1106 18-19 -0.1641773720743629 1106 20-21 -0.5296268727609714 1106 22-23 0.05519205718210429 1106 24-25 0.6594855567968452 1106 26-27 0.3676466488701493 1106 28-29 -0.8852305966627085 1106 30-31 -0.3942223394148172 1106 32-33 0.7752162770097542 1106 34-35 0.6495166425850059 1106 36-37 0.5413943502172174 1106 38-39 0.6176628284878589 1106 40-41 0.17134073291393292 1106 42-43 0.7815253086892184 1106 44-45 0.7563522087741461 1106 46-47 0.9726442763753695 1106 48-49 0.7873555716016014 1106 50-51 0.8249035454624476 1106 52-53 0.196805396183656 1106 54-55 0.7755991912823816 1106 56-57 0.6469331571438701 1106 58-59 -0.37464952299457366 1106 60-61 0.9338579818725563 1106 62-63 1.16818945116907 1106 64-65 1.6321113310642943 1106 66-67 1.32636418493923 1106 68-69 1.5128761638154486 1106 70-71 0.7802144153438988 1106 72-73 0.43070890369138937 1106 74-75 -1.157873272652715 1106 76 -0.15424488008615 1107 1 -0.4956194009811874 1107 2 0.11644693323302846 1107 3 0.24703373289831987 1107 4 0.09436414705780294 1107 5 0.15618194186471257 1107 6 0.5843097860422546 1107 7 -0.13618208859050185 1107 8 0.9793394512994311 1107 9 -1.518055778295171 1107 10-11 -0.2945363096312832 1107 12-13 0.1570568937931327 1107 14-15 -0.5406158785061379 1107 16-17 0.10300357699946261 1107 18-19 1.0335737523634236 1107 20-21 0.7380000280743317 1107 22-23 -0.45816198008498077 1107 24-25 -0.6009342333081023 1107 26-27 0.06777515604513695 1107 28-29 -0.9974173604236825 1107 30-31 -0.4353874711346748 1107 32-33 0.6578678083869249 1107 34-35 0.48921465072379533 1107 36-37 -0.4700963330125987 1107 38-39 0.4612910143949094 1107 40-41 0.9625058646337834 1107 42-43 0.4896640964382044 1107 44-45 0.1220407930534293 1107 46-47 0.5727834924816051 1107 48-49 -0.17598775673422296 1107 50-51 1.3061200800522954 1107 52-53 1.050425014945695 1107 54-55 0.09597828744858816 1107 56-57 0.3889764212261184 1107 58-59 0.19438024783429597 1107 60-61 -0.5807382914193653 1107 62-63 -0.8000799855411458 1107 64-65 0.3896825454570987 1107 66-67 0.813652683547069 1107 68-69 1.3709614069081937 1107 70-71 -0.185174318860426 1107 72-73 0.2833433007785615 1107 74-75 0.29946234525261417 1107 76 -0.8885777136693598 1108 1 -1.366199907431259 1108 2 -0.9330235238461242 1108 3 -0.2719183295320029 1108 4 -0.22208751721994702 1108 5 -0.01946306530203401 1108 6 0.3968197398565536 1108 7 1.0929945318904686 1108 8 0.5734428117071388 1108 9 0.014294118185155469 1108 10-11 -0.11593501961694841 1108 12-13 0.14149908203967243 1108 14-15 0.5709663798152533 1108 16-17 -0.4114991304380311 1108 18-19 -0.005236169598674678 1108 20-21 0.2927758681762569 1108 22-23 -0.5515387120486679 1108 24-25 -0.9906360170316191 1108 26-27 -0.8621499912713091 1108 28-29 0.3673003494027185 1108 30-31 0.2460407021413289 1108 32-33 0.484621670466872 1108 34-35 -0.20590997103126085 1108 36-37 -0.6739902651673972 1108 38-39 0.4279855517416209 1108 40-41 -0.00578128300119829 1108 42-43 0.5121398091313054 1108 44-45 -0.23057307858218223 1108 46-47 0.5007278307520338 1108 48-49 0.2840178174017822 1108 50-51 4.78258106120677E-4 1108 52-53 -0.23968885668991646 1108 54-55 0.37000679517274904 1108 56-57 0.6730383737663317 1108 58-59 -1.4596848899264927 1108 60-61 0.6111015716161461 1108 62-63 -0.1251018839326008 1108 64-65 0.38257552204572676 1108 66-67 -0.4277472208963147 1108 68-69 1.1345384096156295 1108 70-71 1.4906697645923543 1108 72-73 0.264749575663096 1108 74-75 0.7445471517504672 1108 76 0.22095405890589603 1109 1 -0.8613764521979697 1109 2 -1.2908944780285516 1109 3 -0.40264552583296975 1109 4 -0.5192291733779939 1109 5 0.06089632940162204 1109 6 0.19629430900325673 1109 7 -1.4602631393999914 1109 8 -0.11898047538827683 1109 9 0.28410286135455465 1109 10-11 0.7183493435608597 1109 12-13 -0.2559000352364933 1109 14-15 -0.39575282993673966 1109 16-17 -1.2584463771803556 1109 18-19 -0.6596550430749204 1109 20-21 0.4576823414843716 1109 22-23 0.41748713914931557 1109 24-25 0.4561220746035133 1109 26-27 0.10193155559711897 1109 28-29 -0.13562630045015567 1109 30-31 -0.07791369046060481 1109 32-33 0.6360981311646441 1109 34-35 -0.21388075707582033 1109 36-37 0.056148448577872045 1109 38-39 -0.1823654360683875 1109 40-41 -0.23267283407307104 1109 42-43 -0.13452895925877328 1109 44-45 -0.3731277233909225 1109 46-47 -0.9962929763097534 1109 48-49 -0.3084895386301625 1109 50-51 0.3741121378875434 1109 52-53 0.12492862964888474 1109 54-55 -0.13239967813536424 1109 56-57 0.1344402232829367 1109 58-59 -0.1397710605864333 1109 60-61 0.4805792933479651 1109 62-63 -0.5872656081864918 1109 64-65 -0.9016930782064776 1109 66-67 -0.10139160929480084 1109 68-69 0.6006376109607316 1109 70-71 -0.1157720176691015 1109 72-73 -0.5709587051271541 1109 74-75 1.4128163825197007 1109 76 -0.7289198376893147 1110 1 0.19239168083658598 1110 2 0.3181985753975596 1110 3 -0.18180580416768066 1110 4 0.19139707971788766 1110 5 0.1816299064743916 1110 6 0.5836395148058244 1110 7 0.8235792948493312 1110 8 2.02029302384512 1110 9 -1.4014732679059705 1110 10-11 -0.9147382846971936 1110 12-13 -0.32948182554195427 1110 14-15 -0.3259391726658336 1110 16-17 0.1443369699124588 1110 18-19 0.6865296485160641 1110 20-21 0.3066916586208208 1110 22-23 0.8908035679734709 1110 24-25 0.5419340807363113 1110 26-27 -0.751653772861431 1110 28-29 0.09785052464714283 1110 30-31 0.03283050107141605 1110 32-33 1.1980393978234503 1110 34-35 0.7994050077549417 1110 36-37 0.043912335828729 1110 38-39 0.6878315211199677 1110 40-41 0.42594256868669333 1110 42-43 0.684824738111196 1110 44-45 1.6397806384442077 1110 46-47 0.6443014334083657 1110 48-49 0.866082934538845 1110 50-51 0.5601305362835873 1110 52-53 0.032193637314883006 1110 54-55 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0.8774990375376035 2116 56-57 0.8402562408249565 2116 58-59 0.10439725934674726 2116 60-61 -0.677288514942731 2116 62-63 0.8482465563744341 2116 64-65 1.0133325039173613 2116 66-67 0.45711314167105144 2116 68-69 1.1425818523724445 2116 70-71 -1.715458767173125 2116 72-73 1.189582174062572 2116 74-75 -0.32603712066501345 2116 76 0.04689722402103058 2117 1 0.8853329799711922 2117 2 1.507751281059118 2117 3 0.8050047473910702 2117 4 0.01258360875552711 2117 5 0.32905978865560215 2117 6 -0.9094790338335201 2117 7 -1.6662027925242526 2117 8 -1.5320477122616438 2117 9 0.4653810747068974 2117 10-11 0.48600199264615185 2117 12-13 1.5757939538345411 2117 14-15 0.050481429982475845 2117 16-17 0.6185315273100258 2117 18-19 0.5770520132329864 2117 20-21 0.19348657051739337 2117 22-23 0.6008442310951523 2117 24-25 0.7758773608947962 2117 26-27 1.1857875784053817 2117 28-29 0.9378828466156506 2117 30-31 0.6163723632338574 2117 32-33 1.5993791955504832 2117 34-35 0.952465168322135 2117 36-37 0.11893058210127805 2117 38-39 0.6062185506267923 2117 40-41 -0.792152520459382 2117 42-43 -0.1376650961083783 2117 44-45 0.9170718489540519 2117 46-47 0.28758680511101176 2117 48-49 -0.08178485818350367 2117 50-51 0.7377970986858386 2117 52-53 1.1063256360637084 2117 54-55 -0.23269666079157503 2117 56-57 0.5168232121163783 2117 58-59 0.7062850097390552 2117 60-61 1.0403797210029921 2117 62-63 0.5058206355768675 2117 64-65 1.1143202266165204 2117 66-67 -0.27278623984837935 2117 68-69 1.1546052992477343 2117 70-71 1.7879105672679358 2117 72-73 1.7315834664100294 2117 74-75 1.1954250781718727 2117 76 1.2635961993741205 2118 1 -0.04628955462617057 2118 2 -0.16714247507033875 2118 3 -0.33534612980190914 2118 4 -0.12482513048387744 2118 5 -0.5659494736305497 2118 6 0.44226990372776953 2118 7 0.6277930666890015 2118 8 -3.7002944159774565 2118 9 -1.2980492402108297 2118 10-11 -0.3954679866499191 2118 12-13 0.1785508460651215 2118 14-15 -0.8039372253515111 2118 16-17 -0.42233149982303075 2118 18-19 -0.9724739756522709 2118 20-21 -2.6480738522787277 2118 22-23 -0.668116213494585 2118 24-25 -0.527952866848473 2118 26-27 0.45098862449887633 2118 28-29 0.13391640878193556 2118 30-31 -0.5748938457669084 2118 32-33 0.13645558216920506 2118 34-35 0.2880051051206607 2118 36-37 -0.26537256707602097 2118 38-39 -0.6398041147322502 2118 40-41 -1.2212960314106738 2118 42-43 -0.6536834027445337 2118 44-45 0.06344321214464088 2118 46-47 -0.36592196681881717 2118 48-49 -0.9898158051160593 2118 50-51 -0.5349227444001414 2118 52-53 -1.558924445662342 2118 54-55 -0.07401190542489644 2118 56-57 0.7758675621218316 2118 58-59 0.029783974542375802 2118 60-61 -1.1830593113605303 2118 62-63 0.7037611390093588 2118 64-65 -0.3366986248599986 2118 66-67 -0.9745951098494672 2118 68-69 0.6830732081504252 2118 70-71 -0.5256450609330017 2118 72-73 -0.6831490169723367 2118 74-75 0.6195832998129376 2118 76 -0.038299845084850404 2119 1 0.6128841528383688 2119 2 0.3712654002881628 2119 3 -0.0059918161828100835 2119 4 -0.4210037212907878 2119 5 0.5012546190754428 2119 6 1.0515250002319192 2119 7 -0.8906163589369172 2119 8 1.1247579055480088 2119 9 0.39530786388962724 2119 10-11 0.2083611738802773 2119 12-13 -0.5461991733132194 2119 14-15 0.5089454969180807 2119 16-17 -0.4233779303322933 2119 18-19 0.2635154825292716 2119 20-21 -0.9189443274356961 2119 22-23 0.7428209231614886 2119 24-25 0.07734904773178641 2119 26-27 0.06958408221533574 2119 28-29 0.35637978371411094 2119 30-31 0.4525071309015658 2119 32-33 0.6997452496368481 2119 34-35 0.6178244989660939 2119 36-37 0.03417785537185836 2119 38-39 -0.2815373137090731 2119 40-41 0.0746112625975428 2119 42-43 -0.7291039127825201 2119 44-45 -0.6547569273794238 2119 46-47 -0.39712109866906076 2119 48-49 -1.008574040911725 2119 50-51 0.20451605879340917 2119 52-53 -1.1928675508479856 2119 54-55 -1.0825771329266445 2119 56-57 0.6633569040333143 2119 58-59 0.8950269789606367 2119 60-61 0.33643530146018463 2119 62-63 -0.34805654954190857 2119 64-65 0.3959577583661016 2119 66-67 0.4025461970259627 2119 68-69 0.2375651080031531 2119 70-71 -0.7220251865046592 2119 72-73 -1.546841753969474 2119 74-75 -0.705740318511225 2119 76 -0.07504118296563433 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 10.0 13 53.0 14 193.0 15 365.0 16 505.0 17 556.0 18 546.0 19 504.0 20 492.0 21 554.0 22 552.0 23 559.0 24 726.0 25 918.0 26 1085.0 27 1259.0 28 1705.0 29 2037.0 30 2530.0 31 3260.0 32 4476.0 33 5914.0 34 8301.0 35 12709.0 36 20796.0 37 19419.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.285461885112994 21.521464434678563 11.338857392062799 30.854216288145636 2 17.975006942515968 19.433490697028603 36.07442377117467 26.517078589280757 3 18.050541516245488 24.06553735073591 28.84532074423771 29.038600388780893 4 14.26492640933074 17.097472924187727 38.05276312135518 30.584837545126355 5 12.595390169397389 36.06331574562622 35.65342960288809 15.68786448208831 6 28.41210774784782 38.43710080533185 17.377395168008885 15.773396278811441 7 27.586781449597336 30.377117467370173 22.719244654262706 19.316856428769785 8 23.89225215217995 34.162732574284924 20.281033046376006 21.663982227159124 9 23.83893362954735 16.14551513468481 22.24826437100805 37.76728686475979 10-11 24.629825048597613 25.74562621494029 27.465148569841713 22.159400166620383 12-13 23.86503748958623 23.92391002499306 26.59594557067481 25.615106914745905 14-15 21.709525131907803 25.4407109136351 24.118300472091086 28.73146348236601 16-17 22.290474868092197 27.57067481255207 26.548736462093864 23.590113857261873 18-19 21.59178006109414 27.131352402110526 27.12191058039434 24.154956956401 20-21 21.18300472091086 27.75395723410164 26.875867814495972 24.18717023049153 22-23 22.455984448764234 27.12690919189114 27.078033879477925 23.339072479866704 24-25 21.775617883921132 28.053318522632605 26.8208830880311 23.350180505415164 26-27 20.635379061371843 28.454318244931965 27.67453485143016 23.235767842266036 28-29 21.806720355456818 27.223549014162735 28.923076923076923 22.046653707303527 30-31 21.83393501805054 27.42016106637045 27.928353235212438 22.817550680366566 32-33 20.71480144404332 28.315467925576225 27.571785615106915 23.397945015273535 34-35 21.916689808386558 27.741738405998333 27.779505692863093 22.562066092752016 36-37 22.384893085254095 27.445154123854486 27.250763676756456 22.919189114134962 38-39 21.58733685087476 27.861705081921688 28.279922243821158 22.271035823382395 40-41 21.61621771730075 26.819772285476258 28.307692307692307 23.256317689530686 42-43 21.32740905304082 26.618717023049154 28.490419327964457 23.563454595945572 44-45 20.770341571785615 26.55817828381005 28.41599555678978 24.25548458761455 46-47 21.254096084420997 26.086087198000556 28.865315190224937 23.79450152735351 48-49 20.429880588725354 26.317134129408498 29.790613718411553 23.462371563454596 50-51 20.126631491252432 26.55595667870036 29.250208275479032 24.067203554568177 52-53 19.580672035545682 26.765342960288805 29.382393779505694 24.271591224659815 54-55 19.357400722021662 26.881977228547626 29.488475423493476 24.27214662593724 56-57 19.21743960011108 27.190780338794777 29.049708414329352 24.54207164676479 58-59 19.552901971674537 26.535406831435715 28.72257706192724 25.18911413496251 60-61 19.74895862260483 26.231602332685366 29.237434046098304 24.782004998611498 62-63 19.244098861427382 26.500971952235492 28.915301305193005 25.33962788114413 64-65 20.464870869203 26.71535684532074 27.492363232435434 25.32740905304082 66-67 20.305470702582614 26.37434046098306 27.385726187170228 25.934462649264095 68-69 19.828936406553733 25.607331296861986 27.89391835601222 26.669813940572062 70-71 20.66537073035268 26.004443210219385 27.419605665093027 25.910580394334907 72-73 21.03193557345182 25.63287975562344 26.63537906137184 26.6998056095529 74-75 21.142460427658982 25.663426825881704 26.661482921410716 26.5326298250486 76 21.41960566509303 24.446542627048043 28.013329630658152 26.120522077200775 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.0 14 1.5 15 1.5 16 2.0 17 2.0 18 3.5 19 11.5 20 19.0 21 20.0 22 25.0 23 40.5 24 78.0 25 105.0 26 164.5 27 277.5 28 391.0 29 451.0 30 539.5 31 738.5 32 1024.0 33 1199.0 34 1431.0 35 1982.0 36 2495.0 37 2689.0 38 3136.0 39 4130.0 40 5092.0 41 5722.0 42 5937.0 43 6106.5 44 6493.0 45 6712.5 46 6715.0 47 6732.0 48 6553.5 49 6061.5 50 5765.0 51 5317.5 52 4415.0 53 3603.0 54 3246.0 55 2992.0 56 2359.0 57 1721.0 58 1462.0 59 1192.0 60 795.0 61 565.0 62 462.0 63 373.0 64 232.0 65 149.0 66 98.5 67 79.0 68 65.5 69 43.5 70 29.0 71 23.0 72 24.5 73 19.0 74 10.5 75 9.0 76 9.0 77 8.5 78 4.5 79 1.0 80 3.5 81 5.5 82 5.0 83 5.0 84 4.5 85 2.5 86 0.5 87 0.0 88 0.5 89 1.5 90 2.0 91 1.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 1.0 98 2.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6642599277978339 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 90025.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.25826159400167 #Duplication Level Percentage of deduplicated Percentage of total 1 75.92842812103345 41.197445154123855 2 9.953732137739015 10.8014440433213 3 4.542848953854973 7.394612607608996 4 2.505834664046186 5.43848930852541 5 1.678745444867543 4.554290474868092 6 1.1874053146624084 3.865592890863649 7 0.7636244523604798 2.9003054707025826 8 0.6674036768619743 2.896973063038045 9 0.5302378905130409 2.5892807553457375 >10 2.239692093518405 18.293807275756734 >50 0.0020472505425213937 0.06775895584559843 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.004443210219383505 0.0 2 0.0 0.0 0.0 0.004443210219383505 0.0 3 0.0 0.0 0.0 0.004443210219383505 0.0 4 0.0 0.0 0.0 0.007775617883921133 0.0 5 0.0 0.0 0.0 0.007775617883921133 0.0 6 0.0 0.0 0.0 0.007775617883921133 0.0 7 0.0 0.0 0.0 0.009997222993612885 0.0 8 0.0 0.0 0.0 0.009997222993612885 0.0 9 0.0 0.0 0.0 0.015551235767842266 0.0 10 0.0 0.0 0.0 0.015551235767842266 0.0 11 0.0 0.0 0.0 0.01777284087753402 0.0 12 0.0 0.0 0.0 0.018883643432379894 0.0 13 0.0 0.0 0.0 0.018883643432379894 0.0 14 0.0 0.0 0.0 0.018883643432379894 0.0 15 0.0 0.0 0.0 0.022216051096917523 0.0 16 0.0 0.0 0.0 0.024437656206609277 0.0 17 0.0 0.0 0.0 0.025548458761455152 0.0 18 0.0 0.0 0.0 0.026659261316301027 0.0 19 0.0 0.0 0.0 0.02888086642599278 0.0 20 0.0 0.0 0.0 0.03221327409053041 0.0 21 0.0 0.0 0.0 0.03443487920022216 0.0 22 0.0 0.0 0.0 0.042210497084143296 0.0 23 0.0 0.0 0.0 0.047764509858372675 0.0 24 0.0 0.0 0.0 0.051096917522910304 0.0 25 0.0 0.0 0.0 0.053318522632602054 0.0 26 0.0 0.0 0.0 0.055540127742293804 0.0 27 0.0 0.0 0.0 0.06553735073590669 0.0 28 0.0 0.0 0.0 0.08442099416828659 0.0 29 0.0 0.0 0.0 0.10219383504582061 0.0 30 0.0 0.0 0.0 0.11552346570397112 0.0 31 0.0 0.0 0.0 0.1444043321299639 0.0 32 0.0 0.0 0.0 0.17550680366564844 0.0 33 0.0 0.0 0.0 0.20549847264648707 0.0 34 0.0 0.0 0.0 0.24881977228547625 0.0 35 0.0 0.0 0.0 0.3043599000277701 0.0 36 0.0 0.0 0.0 0.40655373507359066 0.0 37 0.0 0.0 0.0 0.6209386281588448 0.0 38 0.0 0.0 0.0 0.9297417384059984 0.0 39 0.0 0.0 0.0 1.3118578172729798 0.0 40 0.0 0.0 0.0 1.8372674257150792 0.0 41 0.0 0.0 0.0 2.539294640377673 0.0 42 0.0 0.0 0.0 3.3857261871702304 0.0 43 0.0 0.0 0.0 4.252152179950014 0.0 44 0.0 0.0 0.0 5.0108303249097474 0.0 45 0.0 0.0 0.0 5.7983893362954735 0.0 46 0.0 0.0 0.0 6.581505137461816 0.0 47 0.0 0.0 0.0 7.355734518189392 0.0 48 0.0 0.0 0.0 8.027770063871147 0.0 49 0.0 0.0 0.0 8.657595112468758 0.0 50 0.0 0.0 0.0 9.256317689530686 0.0 51 0.0 0.0 0.0 9.968342127186892 0.0 52 0.0 0.0 0.0 10.819216884198834 0.0 53 0.0 0.0 0.0 11.7845043043599 0.0 54 0.0 0.0 0.0 12.602054984726465 0.0 55 0.0 0.0 0.0 13.3873923910025 0.0 56 0.0 0.0 0.0 14.003887808941961 0.0 57 0.0 0.0 0.0 14.66370452652041 0.0 58 0.0 0.0 0.0 15.3535129130797 0.0 59 0.0 0.0 0.0 15.97000833101916 0.0 60 0.0 0.0 0.0 16.53318522632602 0.0 61 0.0 0.0 0.0 17.1452374340461 0.0 62 0.0 0.0 0.0 17.75173562899195 0.0 63 0.0 0.0 0.0 18.482643710080534 0.0 64 0.0 0.0 0.0 19.227992224382117 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGGCAA 15 0.0021562008 70.50196 1 AGCGGGC 20 7.893072E-5 69.99278 22 GAGCGGG 20 7.893072E-5 69.99278 21 GACCATA 15 0.0022191373 69.992775 6 GGGAATA 15 0.0022191373 69.992775 21 GAACCTC 15 0.0022191373 69.992775 32 CTCGTTA 25 2.3803872E-4 55.99422 54 GTTAACC 25 2.3803872E-4 55.99422 57 GCCCCAC 20 0.006934168 52.494587 11 GGGACTT 20 0.006934168 52.494587 11 AGACCAT 20 0.006934168 52.494587 5 GTTCCTA 20 0.006934168 52.494587 25 GAATTGA 20 0.006934168 52.494587 27 AAGATAC 20 0.006934168 52.494587 26 TATGAGC 30 5.853384E-4 46.66185 18 TGAGCGG 30 5.853384E-4 46.66185 20 CTAGGGA 30 5.853384E-4 46.66185 4 ACAATTC 35 0.0012502471 39.995872 21 TACTGAC 35 0.0012502471 39.995872 34 CACAATT 35 0.0012502471 39.995872 20 >>END_MODULE