FastQCFastQC Report
Wed 25 May 2016
SRR1780628_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780628_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90025
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT3350.3721188558733685No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT2400.26659261316301025RNA PCR Primer, Index 30 (95% over 22bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1140.1266314912524299No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAG150.002218229270.034
ACCCGAC150.002218229270.035
TCTGGTA150.002218229270.012
GGTCACC252.3791735E-456.00000431
TTCGGTC252.3791735E-456.00000428
TCGGTCA252.3791735E-456.00000429
AATTTAG200.00693134152.542
ACGTTTT200.00693134152.553
AAGAGCC200.00693134152.563
TTATAGA200.00693134152.535
GTTGTCT200.00693134152.58
CCATAGG200.00693134152.51
TCCAATA200.00693134152.550
CCGACCA200.00693134152.537
TAAGGTG200.00693134152.520
TACAGAC200.00693134152.555
TATACCG200.00693134152.566
ATACAGA200.00693134152.554
TAAACGC305.850407E-446.66666415
CAATTCT350.001249612940.022
Produced by FastQC (version 0.11.5)