FastQCFastQC Report
Wed 25 May 2016
SRR1780627_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780627_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGCT11.0869565217391304No Hit
GGGTGGGGATAGTGGGTGGGGGTGGTCATTTAACTTACAAGACCTGTCTC11.0869565217391304No Hit
GCTATCCATTGGTCTTAGGCCCCAAAAATTTTGGTGCAACTCCAAATAAA11.0869565217391304No Hit
ACGTAGGGCAGACCCAGCGACTCGAACATGCCCTGGAGGGTGCCGTCCTC11.0869565217391304No Hit
ACGGGAGCCTGGGTTAGAGGGCGCGGCTGGGGCCTGGGGGGAGACGGGCC11.0869565217391304No Hit
TCTTATACACATCTGACGCTGCCGACGAACTACGAGGTGTAGATAGGGGT11.0869565217391304No Hit
GGCAGGCTCCGTGCCGGCCGAACGGGCCCTGACCGCGCCGTGGGTGCGGT11.0869565217391304No Hit
ATTTACACATCTGGCGCTCCCCACCCAAATCCGCGTGGAGAGCGCGGGGG11.0869565217391304No Hit
GGCGTACGGCCAGGGCTATTGGTTGAATGAGTAGGCTGATGGTTTCGATA11.0869565217391304No Hit
ACGGAGGATTGGATTAGGGCGCCAGCGCATTGTGGGATAATCAGTAGCCT11.0869565217391304No Hit
GCATTGTTCGTTACATGGTCCATCATAGAATTCTCACTGTGATATATCTG11.0869565217391304No Hit
CTATAGCAGCGGTCCCCAACCTTTTGGGCATCAGGGACCAGTCTGTCTCT11.0869565217391304No Hit
GGCTAGGAGGGTGTTGATTATTAAAATTAAGGGGACGTTTATTACTCTTT11.0869565217391304No Hit
GGCACGCTCAGGCGCGGTTACCGCCGCCGGCCACGGCCCGGCCGCGCCCT11.0869565217391304No Hit
TACTAACATTAACGAAAATAACCCCACCCTACTAAACCCCATTAAACGCC11.0869565217391304No Hit
ACATTTTTCACCCCAGACCAACCTATTAGCCCTACTGAAAGTTATTGGTC11.0869565217391304No Hit
CCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACCTCCGGCCT11.0869565217391304No Hit
GACCACGTCCGGGACGAGCACGAGCGCCGGCTCGACGAGGAGTGGGCCGC11.0869565217391304No Hit
TCTCTGATTTGTCCTTGGAAAAAGGTTTTCATCTTCGGTTTACAAGACTG11.0869565217391304No Hit
GATTATGAGGGCGTGATCATGAAAGGTGATAAGCTCTTCTATGATAGGGG11.0869565217391304No Hit
ATTATACAGAAAATGTAGCTATTAATAAGAAAGTACTCGACAGTGGGACT11.0869565217391304No Hit
ACGCTGGACAGGCCCGGCCCGCGCTCGCCGGAGAAGGTGAAGAACACGGA11.0869565217391304No Hit
CACGGGAGGACATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAG11.0869565217391304No Hit
ATGATAATCATATCTACCAAATGCCCCTCATTTACATAAATATTATACTA11.0869565217391304No Hit
GCGCTCGCCCACGCGGTGGCGGGCCGGCTCGAGGGGCTGTCTCTTATACA11.0869565217391304No Hit
CTTATACACATCTGANGNNNNCGNCGAACTTCTAGGTGTNGATCTCGGTG11.0869565217391304No Hit
GCCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTT11.0869565217391304No Hit
CACCAATAGGATCCTCCCGAATCAACCCTGACCCCTCTCCTTCATAAATT11.0869565217391304No Hit
CCTCTCAACGTACGTGCGCTCCTCTCAACGTACGTGCGCTCCTCTCAACG11.0869565217391304No Hit
GAGGAGGGCATCGTCGCCGGCGGCGGCGTGGCCCTGATCCTGTCTCTTAT11.0869565217391304No Hit
GAGTTCGACCGCGAGGACGGCTCGGACGGCCCCGAGGTCGAGATCGACGC11.0869565217391304No Hit
TGCCGGTAGGCGACCTGCAAAGCGGTCGCCGCGGTGTACGGCTGCTGTCT11.0869565217391304No Hit
GTCGAGGCGTCGTCCGTGGCGGGGGGGGTCGCCCCCGCCGCGGGGGGGGC11.0869565217391304No Hit
GTGTAACCGATGCCGTCCGGCGTCATGGCCTTGCGCACGGTGGGGTCCAG11.0869565217391304No Hit
CCCTTGAGGAGGCCGCCGGCCTCCTCAAGGGCGAGCTGCGGGACCGGGGG11.0869565217391304No Hit
AGATAACGTGCTGAGACAGTCTGGGGATGAGAGATGAGAGAGAAAGCTGT11.0869565217391304No Hit
GTGCTGTGAGATACGTTAACGCTTTCTCGACACCAGGGGTCTGGACCTGT11.0869565217391304No Hit
GCCTGGTGCTGCCCGGCAGCTCGGGCCGTCCCGTGGCCTGTCCCTTATAC11.0869565217391304No Hit
CCTACGAGCAGCCCTACCTGCTGGCGCGCACGCTGACCTCGCTGGACCTG11.0869565217391304No Hit
CCCCGAGGGAGATCACCACGGCGGCGAGCAGGATCTGTCTCTTATACACA11.0869565217391304No Hit
CTTCTACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG11.0869565217391304No Hit
CACGACCACCAAGTGGATCCTGCGCGGCGACGGGCAGATCCGCCGCTGAG11.0869565217391304No Hit
TACTTCGGTTGGGCGGGGTTGGGGTTTCTTTCAGGTGTCGTTCATTTGGG11.0869565217391304No Hit
TCTCTTATACACATCTGACGCTGCCGACGATTGGTACGGTGTAGATCTCG11.0869565217391304No Hit
ACATGGTCCGTCCGGCCGTCGAGGATGCGAGTGCGATCCACGATGACGGC11.0869565217391304No Hit
AGCTGGGAGTTTTTGAGGGCCACTGAGACTATTTTGGGTTCTTCTTAGGT11.0869565217391304No Hit
CCTCAGGATACTCCTCAATAGCCATCGCTGTAGTATATCCAAAGACAACC11.0869565217391304No Hit
CCGTGTAGGTGAGCTGCGGGTCGGAGGCGATGCTGTCTCTTATACACATC11.0869565217391304No Hit
CCCCTAATGACGTCCGGCCTAGACATGTGATTTCACTTCCACTCAATACC11.0869565217391304No Hit
ATTTGTAATAATCTTCTTCATAGTAATACCCATCATAATCGGAGGCTTTG11.0869565217391304No Hit
AGGGTGTTGTGAGTGTAAATCAGTGCGATGAGTAGGGGAAGGGAGCCTAC11.0869565217391304No Hit
GAGGAGAGCAAGGTGGGGCGGGAGGGGGGGCTGAGGACGTTTGTTCAAAC11.0869565217391304No Hit
GTGCCGTCCCGCGCGGTGAGGAACACGACGGGGAAGTGACGGCCGGCGGA11.0869565217391304No Hit
AGTGAGGTCAGCCTGGCAGAGCCTGGCCTGGACACACCTTGGAGGCTGTC11.0869565217391304No Hit
GCCGGGTACGCGGCGCGCACCACGGCCTCGCCGCAGGCGAGGTGGTCCGG11.0869565217391304No Hit
CAGACGCAGATCGGAGGCTTCTAGGGAGAACCAGGGAGTAGTGCCGGGGG11.0869565217391304No Hit
GTGATATAGGCCTCGCCCGATGTGTAGGAAGAGGCAGATAAAGAATATTG11.0869565217391304No Hit
CTTCTCCTTACACCTAGCAGGTGTCTCCTCTATCTTAGGGGCCATCAATT11.0869565217391304No Hit
GTCGTGAACTCCTCGATGGCCCACTCGCCGTTGAAGCTGTCTCTTATACA11.0869565217391304No Hit
GGCGTGTCCTTGTCGCCGTTGGCCGCGAGCTTCCGCCCCAGCGTCGTTGC11.0869565217391304No Hit
AGTGTAGTGGCTTTTGCCCTTGGTCCCCAATACTTGGGGGGCTGAGGCAG11.0869565217391304No Hit
ACGTCCACGGGCACGATTGGGCGCTCCACCACGCCGTGCGCGGTCTGTCT11.0869565217391304No Hit
CCTACTACTATCTCGCACCTGAAACAAGCTAACATGACTAACACCCTTAA11.0869565217391304No Hit
ACGGGGGTGGTGGCGGGGCGGGTGGTTCAGTGGGGTTCTGTTGTCTGAGC11.0869565217391304No Hit
GATTATCTACTCTGCCAGGCACCCCCGCGTGGGGAGTGCAGGTCGTTGCG11.0869565217391304No Hit
GCACGGTCCGGAGCCGGGAGCGCTCAGAGGATGTTGTCCTTGGACTCCTC11.0869565217391304No Hit
CATCTGACGCTGCCGACGAAGCCGGCGGGGACCACTTGGGTGTTCTCCGT11.0869565217391304No Hit
GGCTAGAGGTGGCTAGAATAAATAGGAGGCCTAGGTTGAGGTCTGTCTCT11.0869565217391304No Hit
ATACCTGGCCCTCCCCCGAGTTCTGTCGACTACCTGCCTCAGGGCCTGGT11.0869565217391304No Hit
GCTTATGAGCATGCCTGTGTTGGGTTGTGACTGAGGGTAATCTCTGGCTC11.0869565217391304No Hit
ACATATGACGCACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAA11.0869565217391304No Hit
ACCCGCGCTGGCGGCTGCGCACCGAGCTCGTGGAGGACGACTTCCGGCCT11.0869565217391304No Hit
GGATTACCCTTAAACCAACTGACCAGCTCAGGTCAATTCCCCAAACCACA11.0869565217391304No Hit
GGGTTCGATTCTCATAGTCCTAGAAATAAGGGGGTTTAAGCTCCTATTAT11.0869565217391304No Hit
CCCCAGCCCGATGCCGAGGATCAGCGCCACCGCGGGCACGAGGCTGTCTC11.0869565217391304No Hit
AGCCAGCCGCCCTTGCGGTGGCCCGCCTCGGAGAAGGAGTACGGCAGCGG11.0869565217391304No Hit
GAGCTACTGGTGGTTGACCTTTTTGGAATTTGTCTTTTAGTGAAATGGTA11.0869565217391304No Hit
CCCCCGAGCAATCTCAATTACAATATATACACCAACAAACAATGTTCAAC11.0869565217391304No Hit
GCCTCCGGTGACGCGCGTGGCCTCTTTGAGAAGGTCCGTGCGGAGGCCCT11.0869565217391304No Hit
GCCAGGCGATCTACCTGCTGGGCGAGACCGCGGACGAGCTGGACCTGTCT11.0869565217391304No Hit
ATATGTGGCACCCCCTGGGACCTGAGTCAGGCCTGCTTCCCAGGCTGGAA11.0869565217391304No Hit
GTCTAAAACGGCGGCCACTCCCCTCGAAAAAAACAAGAAAATTAGGTATT11.0869565217391304No Hit
GTTGGGGTCCTTCCTTTTTGGTCTGGCTTTTAGGTATACGGGGTCTGCGC11.0869565217391304No Hit
GTCCCCAACCATGCGGGGCATTAGTCAGGCCCCTCTTAAGAAATCAGGAA11.0869565217391304No Hit
GATTTCTATGGCCCCTTTAGAATTTTGTGTCTCAGTGAGTGTGTTTACAG11.0869565217391304No Hit
TGCCAACACAGCAGCCATTCAAGCAATCCTATACAACCGCTGTCTCTTAT11.0869565217391304No Hit
GTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGTTCTCCTT11.0869565217391304No Hit
CTCCAGCATGGGGCGGTGCTCGTAGAAGGTCTGCCTGTCTCTTATACACA11.0869565217391304No Hit
GCTAAATCCCCTAGGATTACCACCCCTAACCACATCCGGATTCCTCTCAT11.0869565217391304No Hit
GTGTTTAAGGGGTTGGCGAGGGTATAATTGTCTGGGCCGCCTAGGAGGCC11.0869565217391304No Hit
CTACTGCTCGCAGTGCGCCGATCAGGGCGTAGCTGTCTCTTATACACATC11.0869565217391304No Hit
CCACGGTTCCGGCGAGCATCGGGTCGGTATCGATGCGGCGCAGCTGCGCC11.0869565217391304No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCACTA50.044.013
TTCATCG50.044.025
AATCCAT50.044.06
AATCTAA50.044.037
ATCCATT50.044.07
TAAATCT50.044.035
AAATCTA50.044.036
AAATCCA50.044.05
CCAAAAT50.044.02
GGCGTAA50.044.031
TCGGCGT50.044.029
ATCTAAC50.044.038
TTTCACT50.044.012
CATTTCA50.044.010
AACTTTC50.044.042
ACTTTCT50.044.043
CGGCGTA50.044.030
TAACTTT50.044.041
TCTAACT50.044.039
CCATTTC50.044.09
Produced by FastQC (version 0.11.5)