FastQCFastQC Report
Wed 25 May 2016
SRR1780627_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780627_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTTCTGCTTG22.1739130434782608Illumina PCR Primer Index 3 (96% over 28bp)
AAGTACAGCAATCAACCCTCAACTATCACACATCAACTGCAACTCCAAAG11.0869565217391304No Hit
CTTCAACGGCGAGTGGGCCATCGAGGAGTTCACGACCTGTCTCTTATACA11.0869565217391304No Hit
GATTGAGGGGGAACCTCTCTATCTCTGCCTCTCTGACAGCTTTCTCTCTC11.0869565217391304No Hit
GGACACAGCAAACACATGCAGGCCAGAGGCACTCTTCTTAATTCCAGGTT11.0869565217391304No Hit
GGCCGCGGCCCTGCTCGAGAACCGCACCCACGGCGCGGTCAGGGCCCGTT11.0869565217391304No Hit
CTACGCCCTGATCGGCGCACTGCGAGCAGTAGCTGTCTCTTATACACATC11.0869565217391304No Hit
ACTTTAATAGTGTAGGAAGCTGAATAATTTATGAAGGAGAGGGGTCAGGG11.0869565217391304No Hit
CAATTAGATGGGCATGGTGACATGCACTCGTAGTCCCAGCTACTCAGGAG11.0869565217391304No Hit
GGCTTCCAGCCTGGGAAGCAGGCCTGACTCAGGTCCCAGGGGGAGCCACA11.0869565217391304No Hit
AGTATAAATAGTACCGTTAACTTCCAATTAACTAGTTTTGACAACATTCA11.0869565217391304No Hit
GGTGCAGCTGGTGGAGTCCCACTGTCGAGTACTTTCTTATTAATAGCTAC11.0869565217391304No Hit
TCCCCATGGATGACCCCCCTCAGATAGGGGTCCCTTGACCACCATCCTCC11.0869565217391304No Hit
CCTCCAAGGTGTGTCCAGGCCAGGCTCTGCCAGGCTGACCTCACTCTGTC11.0869565217391304No Hit
GTGTGGTTTGGGGAATTGACCTGAGCTGGTCAGTTGGTTTAAGGGTAATC11.0869565217391304No Hit
CCCAACAATGACTAATCAAACTAACCTCAAAACAAATAATAACCATACAC11.0869565217391304No Hit
CCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTCT11.0869565217391304No Hit
CCCTACAAACAACTAACCTGCCACTAATAGTTATGTCATCCCTCTTATAC11.0869565217391304No Hit
GCACGGGCGTGCTGGCCGCGCTCGCCCTCGCGGCTTGCGGCACCACGGCC11.0869565217391304No Hit
ATCTCCGACCCACGAGACAAACGGTCATCTCGTATGCCGTCTTCTGCTTG11.0869565217391304TruSeq Adapter, Index 7 (96% over 28bp)
ATATATCACAGTGAGAATTCTATGATGGACCATGTAACGAACAATGCCTG11.0869565217391304No Hit
CTTATACACATCTCCGAGCCCACGAGAGGATCCAAAATCTCGTATGCCGT11.0869565217391304No Hit
ATCCTCATCCTAGCAATAATCCCCATCCTCCATATATCCAAACAACAAAG11.0869565217391304No Hit
CCTCGTGCCCGCGGTGGCGCTGATCCTCGGCATCGGGCTGGGGCTGTCTC11.0869565217391304No Hit
TCTTATACACATCTCCGAGCCCACAGACATGATGATATCTCGTATGCCGT11.0869565217391304No Hit
GGCCGGGCCGGGGCCGGGCCGAAGGGGTCCTGGTGTCCATCGTAGCCCGG11.0869565217391304No Hit
GGTTTAATAGTTTTTTTAATTTATTTAGGGGGAATGATGGTTGTCTTTGG11.0869565217391304No Hit
TCTTATACACATCTCCGACCCACGAGACCCAACACGATCTCGTATGCCGT11.0869565217391304TruSeq Adapter, Index 1 (95% over 21bp)
ATACACATCTACGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTT11.0869565217391304Illumina PCR Primer Index 3 (95% over 21bp)
TCCAAATAAACACAATTAGAAATGACAAAAGGGATATTACCACCGAGCCC11.0869565217391304No Hit
TCTCTTATACACATCTCCGAGCCCACGAGCTTGACCCTATCTCGTATGCC11.0869565217391304No Hit
AGGCGCCGCACCCCCCGGTCCCGCAGCTCGCCCTTGAGGAGGCCGGCGGC11.0869565217391304No Hit
TCTTATACACATCTCCGAGCCCACAGACCGGTTCCCATCTCGTATGCCGT11.0869565217391304No Hit
CCCCTCGAGCCGGCCCGCCACCGCGTGGGCGAGCGCCTGTCTCTTATACA11.0869565217391304No Hit
GATCAGGGCCACGCCGCCGCCGGCGACGATGCCCTCCTCCTGTCTCTTTA11.0869565217391304No Hit
AAGTAATTCTACTGCCTCAGCCTCCCAAGTAGTTGGGACCACAGGCACAA11.0869565217391304No Hit
GCGCGCCACTGTGCGGCCCACTCCTCGTCGAGCCGGCGCTCGTGCTCGTC11.0869565217391304No Hit
GCCGCACCCCGGCCGTCGACGGCGGCGCGCACCGCGCCTGAGCGCGCCCT11.0869565217391304No Hit
CTGCAGCCCACCATGCCTGAGCCTTCCCCTGCCTCCGTGGGTTCCTGTGC11.0869565217391304No Hit
GAGCAGAACCCAACCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGC11.0869565217391304No Hit
GCCACGGGACGGTCCGAGCTGCCGGGCAGCACCAGGCCTGTCTCTTATAC11.0869565217391304No Hit
CCGCGCGGCCGCGCAGGTGGAGGAGTCCAAGGACAACATCCTCTGAGCGC11.0869565217391304No Hit
CGCTGGACCCCACCGTGCGCAAGGCCATGACGCCGGACGGCATCGGTTAC11.0869565217391304No Hit
GAGTAGATTAGGCGTAGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGC11.0869565217391304No Hit
CAATGATACCATTTCACTAAAAGACAAATTCCAAAAATGTCAACCACCAG11.0869565217391304No Hit
CCTCACCGGACCTGGCGTCGATCTCGACCTCGGGGCCGTCCGAGCCGTCC11.0869565217391304No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC11.0869565217391304No Hit
GGACATCCCCGTGCTCGGGCCCATCCTGTTCGACCAGACGATCCTGGGCC11.0869565217391304No Hit
CACCCGGACCACCTCGCCTGCGGCGAGGCCGTGGTGCGCGCCGCGTACCC11.0869565217391304No Hit
TCCAGGCGTGGTCCGTGTTCTTCACCTTCTCCGGCGAGCGCGGGCCGGGC11.0869565217391304No Hit
GCTATAATCAACCGCGATAGTATTATTCCTTCTAGGCATAGTAGGGAGGC11.0869565217391304No Hit
GCCGGAAGTCGTCCTCCACGAGCTCGGTGCGCAGCCGCCAGCGCGGGTCT11.0869565217391304No Hit
ACCTCAACCTAGGCCTCCTATTTATTCTAGCCACCTCTAGCCCTGTCTCT11.0869565217391304No Hit
CTGTAAACACACTCACTGAGACACAAAATTCTAAAGGGGCCATAGAAATC11.0869565217391304No Hit
GCCGAGACGGACCCGGGCACCCACGGCCTGCCCGCGTCCGAGCCGATGGC11.0869565217391304No Hit
CCCACGAGACGGTAAACCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA11.0869565217391304Illumina PCR Primer Index 1 (96% over 27bp)
ACCGCGCACGGCGTGGTGGAGCGCCCGATCGTGCCCGTGGACGTCTGTCT11.0869565217391304No Hit
TCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTCTTCTGCTTG11.0869565217391304Illumina PCR Primer Index 6 (96% over 29bp)
ATCCTGCTCGCCGCCGTGGTGATCTCCCTCGGGGCTGTCTCTTATACACA11.0869565217391304No Hit
CTCCACTATAGTAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTAAGAGTC11.0869565217391304No Hit
GAGCACGTGCGGTACTCCGGCTCACCGCTGCCGTACTCCTTCTCCGAGGC11.0869565217391304No Hit
TTCTACACCCTAGTAGGCTCCCTTCCCCTACTCATCGCACTGATTTACAC11.0869565217391304No Hit
GTCCCTAACAAGCTAGGAGGCGTCCTTGCCCTATTACTATCCATCCTCAT11.0869565217391304No Hit
ATCTCCAGCATTCCCCCTCAAACCTAAGAAATATGTCTGATAAAAGAGTT11.0869565217391304No Hit
GCAGACCTTCTACGAGCACCGCCCCATGCTGGAGCTGTCTCTTATACACA11.0869565217391304No Hit
CTTATCACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTC11.0869565217391304No Hit
GAACTGGCCATCGAGGGCCTCCGCACGGACCTTCTCAAAGAGGCCACGCG11.0869565217391304No Hit
CTAATACACCAGTCTTGTAAACCGAAGATGAAAACCTTTTTCCAAGGACA11.0869565217391304No Hit
GAGCGCACGTACGTTGAGAGGAGCGCACGTACGTTGAGAGGAGCGCACGT11.0869565217391304No Hit
CATTTCTCTACTCAGAAACCTGAAACATCGGCATTATCCTCCTGCTTGCA11.0869565217391304No Hit
GCCTAACTGGGAGGCACCCCCCAGTAGGGGCAGACTGACACCTGTCTCTT11.0869565217391304No Hit
CGCCCAGGTCATGCGCGTCATCACGCGGTCCTGGCGCTGTCTCTTATACA11.0869565217391304No Hit
CTCTTATACACATCTCCGAGCCCACGGAGACTAACAACAATCTCGTATGC11.0869565217391304No Hit
GACCCGGACGCCGCCGTCATCGTGGATCGCACTCGCATCCTCGACGGCCG11.0869565217391304No Hit
CATCGCCTCCGACCCGCAGCTCACCTACACGGCTGTCTCTTATACACATC11.0869565217391304No Hit
TCACATGGCTAGGCCGGAGGTCATTAGGAGGGCTGAGAGGGCCCCTGTTA11.0869565217391304No Hit
CCCCTGGGGCGAGGACGGCACCCTCCAGGGCATGTTCGAGTCGCTGGGTC11.0869565217391304No Hit
GTTAGTAGTATGGTGATGCCAGCAGCTAGGACTGGGAGAGATAGGAGAAG11.0869565217391304No Hit
GGCTGTTAGAAGTCCTAGGAAAGTGACAGCGAGGGCTGTGAGTTTTAGGT11.0869565217391304No Hit
CAGCCGTACACCGCGGCGACCGCGTTGCAGGTCGCCTACCGGCACTGTCT11.0869565217391304No Hit
ATACACATCTCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTTC11.0869565217391304RNA PCR Primer, Index 10 (95% over 22bp)
GCGCAGCTGCGCCGCATCGATACCGACCCGAAGCTCGCCGGAACCGTGGC11.0869565217391304No Hit
TCTTTATACACATCTCCGACCCACGAGACAAACGGTCATCTCGTATGCCG11.0869565217391304No Hit
CGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCACTGTCTCTTAT11.0869565217391304No Hit
GGAAAAGTATTAAAACAGATGTCCAAATCCGAGCCCACGAGACGATCCAA11.0869565217391304No Hit
GCTCTGAGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA11.0869565217391304No Hit
GTCCAGCGAGGTCAGCGTGCGCGCCAGCAGGTAGGGCTGCTCGTAGGCTG11.0869565217391304No Hit
CTCCCGGACATGGACGGCTTCACCGTGACCCGCCGCCTGCGCTCCGCCGG11.0869565217391304No Hit
CTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTC11.0869565217391304No Hit
ACCTTAACAATGAACAAGATATTCGAAAAATAGGAGGACTACTCAAAACC11.0869565217391304No Hit
GTCCAGCTCGTCCGCGGTCTCGCCCAGCAGGTAGATCGCCTGGCCTGTCT11.0869565217391304No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGATA50.044.040
GTATGCA50.044.03
AACGTCG50.044.026
TGCATCG50.044.06
GGCATTC50.044.034
GGGCATT50.044.033
CGGGGTA50.044.011
GTGTATG50.044.01
ACGTCGG50.044.027
TTCCGGA50.044.038
CGGGGCA50.044.031
GGGTAGT50.044.013
TAACGTC50.044.025
TATGCAT50.044.04
GTCCGAG50.044.018
ATAGGCC50.044.044
CATTCCG50.044.036
CGGATAG50.044.041
TAGTCCG50.044.016
GGATAGG50.044.042